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Preparation of Lactobacillus plantarum/Lactobacillus casei/Bacillus licheniformis composite microbial inoculant

A technology of Bacillus licheniformis and Lactobacillus plantarum, which is applied in the field of probiotic fermentation, can solve the problems of large environmental pollution, cumbersome operation of machinery and equipment, and difficult post-processing, and achieve low capital investment, maintenance of intestinal microecological balance, and low production costs. low effect

Active Publication Date: 2014-07-30
内蒙古科拓微生态科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment

Method used

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  • Preparation of Lactobacillus plantarum/Lactobacillus casei/Bacillus licheniformis composite microbial inoculant
  • Preparation of Lactobacillus plantarum/Lactobacillus casei/Bacillus licheniformis composite microbial inoculant
  • Preparation of Lactobacillus plantarum/Lactobacillus casei/Bacillus licheniformis composite microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Mixed solid-state fermentation of probiotic preparations

[0029] (1) Preparation of bacterial seed solution

[0030] Bacillus licheniformis BL-09 was inoculated into the liquid seed medium from a frozen glycerol tube, placed on a shaker with a rotation speed of 150-180r / min and a temperature of 30-37°C, and cultivated for 20-24h. Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 are respectively inoculated into liquid seed medium, placed in a constant temperature incubator for static cultivation, the cultivation temperature is 30-37° C., and the cultivation time is 20-24 hours. The composition of the liquid seed culture medium is (g / L): peptone 10-20, glucose 10-20, the rest water, pH 7.0-7.2.

[0031] (2) Mixed solid-state fermentation

[0032] First inoculate Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 in the solid-state fermentation medium with 1%-5% inoculation amount (v / w), control the temperature at 30-37°C, and relative hum...

Embodiment 2

[0038] Embodiment 2: the impact of Lactobacillus plantarum HM-10 inoculum size on the number of viable bacteria

[0039] Lactobacillus plantarum HM-10 was inoculated into the liquid seed medium, placed in a constant temperature incubator for static cultivation, the cultivation temperature was 37°C, and the cultivation time was 20h. The composition of the liquid seed culture medium is (g / L): 10 peptone, 20 glucose, the rest is water, and the pH is 7.0-7.2.

[0040] The solid-state fermentation medium is composed of soybean meal, bran and corn flour, and the mass ratio of the soybean meal, bran and corn flour is: soybean meal, bran and corn flour=10:10:0.5. The moisture content is controlled at 60%, and it is sterilized at 115°C for 20 minutes.

[0041] The solid medium was inoculated with 1% (v / w), 3% (v / w), and 5% (v / w) Lactobacillus plantarum HM-10, and the Lactobacillus plantarum in the fermentation product was measured after 48 hours of single-bacteria fermentation. The n...

Embodiment 3

[0042] Embodiment 3: the impact of Lactobacillus casei HM-09 inoculum size on the number of viable bacteria

[0043] Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 were respectively inoculated into the liquid seed medium, placed in a constant temperature incubator for static cultivation, the cultivation temperature was 37°C, and the cultivation time was 20 hours. The composition of the liquid seed culture medium is (g / L): 10 peptone, 20 glucose, the rest is water, and the pH is 7.0-7.2.

[0044] The solid-state fermentation medium is composed of soybean meal, bran and corn flour, and the mass ratio of the soybean meal, bran and corn flour is: soybean meal, bran and corn flour=10:10:1. The moisture content is controlled at 60%, and it is sterilized at 115°C for 20 minutes.

[0045] In solid state fermentation, inoculate 1% (v / w) Lactobacillus plantarum HM-10, and at the same time inoculate 1% (v / w), 3% (v / w), 5% (v / w) Lactobacillus casei HM -09, other conditions ...

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Abstract

The invention belongs to the technical field of fermentation of probiotics, and relates to a mixed solid state fermentation using bean pulp, bran and corn flour as matrixes and probiotics Lactobacillus plantarum HM-10, Lactobacillus casei HM-09 and Bacillus licheniformis BL-09 as strains, particularly a probiotic solid fermentation technique which can be widely used in the field of microecology. The method can be used for producing a fermented feed product with special sourness, and the fermented product has the advantages of high viable count and high degradation ratio of bean pulp proteins. The preparation method is low in production cost, can enhance the economic benefit and is simple to operate.

Description

technical field [0001] The invention belongs to the technical field of probiotic fermentation, and in particular relates to a kind of probiotics Lactobacillus plantarum HM-10 (Lactobacillus plantarum HM-10), Lactobacillus casei HM-09 (Lactobacillus casei HM-09) and Bacillus licheniformis BL-09 (Bacillus licheniformis BL-09) are mixed solid-state fermentation technology, which can be widely used in animal microecology and other aspects. Background technique [0002] At present, probiotic products mostly use probiotics to directly ferment with liquid milk and soy milk to obtain functional yogurt or soy milk, and the rest are fermented in liquid state to directly collect probiotic cells and make probiotic powder, tablets or capsules. The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment. Therefore this application adopts Lactobacill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/25C12R1/245C12R1/10A23K10/18
Inventor 高鹏飞贺润晶姚国强
Owner 内蒙古科拓微生态科技发展有限公司
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