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Lactobacillus paracasei, Lactobacillus paracasei preparation, and applications of Lactobacillus paracasei preparation in pig feed

A Lactobacillus paracasei technology, applied in preparations and its application in pig feed, in the field of Lactobacillus paracasei, can solve the problems of cumbersome production operations, long fermentation process, etc., to reduce harmful bacteria emissions, live The effect of reducing the number of bacteria and reducing the rate of diarrhea

Active Publication Date: 2017-09-15
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Lactobacillus paracasei involved in the above patents is mainly used for the preparation of fermented feed. The preparation process requires fermentation equipment, the fermentation process takes a long time, and the production operation is relatively cumbersome. At present, there is no freeze-dried preparation involving Lactobacillus paracasei The preparation method also does not involve the method that the freeze-dried preparation with stable quality is directly added to the feed in proportion and can improve the reproductive performance of sows

Method used

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  • Lactobacillus paracasei, Lactobacillus paracasei preparation, and applications of Lactobacillus paracasei preparation in pig feed
  • Lactobacillus paracasei, Lactobacillus paracasei preparation, and applications of Lactobacillus paracasei preparation in pig feed
  • Lactobacillus paracasei, Lactobacillus paracasei preparation, and applications of Lactobacillus paracasei preparation in pig feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Isolation, screening and identification of Lactobacillus paracasei

[0039] Choose healthy nursery pigs for slaughter, take out the intestines and scrape the intestinal mucosa aseptically, mix them with sterile normal saline, and dilute ten times to 10 -5 , 10 -6 , 10 -7 , Each 0.1mL was spread on the MRS plate medium, and the anaerobic tank was incubated at 37°C for 24 to 48 hours. Pick out the milky white colonies for preliminary screening. Anyone with a positive Gram stain, H 2 O 2 Strains with negative enzyme test and negative contact enzymes can be preliminarily identified as lactic acid bacteria. The strains obtained in the preliminary screening will be tested for acid and bile salt resistance, bacteriostasis and other characteristics. Finally, a lactic acid bacteria strain ZLP019 with excellent performance is screened out and sent to China The microbial species collection center (CGMCC) conducted identification, and comprehensive analysis of experimental da...

Embodiment 2

[0045] Example 2 Study on the characteristics of Lactobacillus paracasei

[0046] 1. The growth curve of Lactobacillus paracasei

[0047] The activated Lactobacillus paracasei obtained in Example 1 was connected to the MRS liquid medium at an inoculum of 1%, and the OD was detected by an automatic microbial growth curve analyzer 600 Value, draw a growth curve, and the result is as figure 2 As shown, the abscissa is the culture time, the unit is hour, and the ordinate is the absorbance OD 600 value. Experimental results showed that Lactobacillus paracasei grew rapidly in MRS medium, entering the logarithmic phase at about 4h, and entering the stable phase at about 11h.

[0048] 2. Determination of acid resistance

[0049] The activated Lactobacillus paracasei was inoculated into MRS liquid medium with pH 2, 3, and 4 at 1% inoculation amount, and incubated at 37°C for 2, 4, and 6 hours respectively. With 0h as the control, the number of viable bacteria was determined. Calculate the su...

Embodiment 3

[0070] Example 3 Preparation of Lactobacillus paracasei preparation

[0071] 1. Fermentation culture of Lactobacillus paracasei

[0072] The Lactobacillus paracasei ZLP019 (preservation number: CGMCC No. 11532) obtained in Example 1 was inoculated into MRS liquid medium at an inoculum of 1%, cultured at 37°C for 16-24 hours, and activated for 2 generations. Then the seed culture was obtained; the seed culture was inoculated into a 50L fermentor containing 30L liquid medium with 1% of the inoculum. The temperature was set at 37℃, the rotation speed was 100rpm, and the pH was maintained at 5.5. When the fermentation reached 14 hours Start feeding, add 200mL feeding liquid to the fermenter at a time, and then feed it every 2 hours for a total of 5 feedings; when the pH of the fermentation broth no longer decreases, stop the fermentation.

[0073] Among them, the composition of the liquid medium is: glucose 30g / L, peptone 10g / L, beef extract 10g / L, sodium acetate 5g / L, yeast extract pow...

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Abstract

The present invention discloses Lactobacillus paracasei ZLP019, which has a preservation number of CGMCC No.11532, and has characteristics of good acid resistance, good cholate resistance and good bacterial inhibition. According to the present invention, with the application of the Lactobacillus paracasei ZLP019 to prepare the freeze-drying preparation, the live bacteria survival rate is high, and the preparation is conveniently used in feed enterprises and farms; and after the preparation is directly added to sow feed according to the ratio requirement, the reproductive performance of sows can be improved, the daily weight gain and the survival rate of suckling piglets can be improved, the intestinal health of sows can be improved, the harmful bacteria emission in feces can be reduced, and the environmentally friendly breeding environment can be easily achieved.

Description

Technical field [0001] The invention relates to the technical field of biological feed, in particular to a Lactobacillus paracasei, a preparation and its application in swine feed. Background technique [0002] In pig production, sows are responsible for the important task of breeding piglets and suckling piglets. They have a long use life and play a great role. However, sows in large-scale pig farms often suffer from constipation, decreased appetite, poor farrowing performance, low lactation, and low piglet survival rates, which seriously affect the production performance and economic benefits of the farm. [0003] Lactic acid bacteria can regulate the microecological balance of animal intestines, promote the digestion and absorption of nutrients, and promote the health of the body. Reasonable feeding and use are expected to improve the comprehensive production capacity of sows. [0004] Lactobacillus paracasei (Lactobacillus paracasei) is one of the populations of Lactobacillus (L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/30C12R1/245
CPCC12N1/205C12R2001/245
Inventor 季海峰刘辉王四新张董燕王晶张伟王雅民
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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