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Pediococcus pentosaceus 368 microbial agent as well as preparation method and application thereof

A technology for the preparation of Pediococcus pentosaceae and strains, applied in the biological field, can solve the problems of lack, reduce the diarrhea rate and constipation rate of pigs, and achieve the effects of reducing the ratio of material to weight, improving disease resistance, and reducing the content of ammonium nitrogen

Active Publication Date: 2021-10-01
YUNNAN ANIMAL SCI & VETERINARY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is a lack of Pediococcus pentosaceae 368 and its application that can reduce the diarrhea rate and constipation rate of pigs

Method used

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  • Pediococcus pentosaceus 368 microbial agent as well as preparation method and application thereof
  • Pediococcus pentosaceus 368 microbial agent as well as preparation method and application thereof
  • Pediococcus pentosaceus 368 microbial agent as well as preparation method and application thereof

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preparation example Construction

[0030] The preparation method of Pediococcus pentosaceae 368 bacterial agent of the present invention, comprises the steps:

[0031] (1) Strain preparation: inoculate in MRS liquid medium, culture at 32-38° C. for 18-20 hours, and centrifuge at 8000-10000 rpm to obtain bacteria.

[0032] (2) Freeze-dry the cells.

[0033] In step (1), the MRS culture medium is: peptone 10.0g; beef extract 5.0g; yeast powder 4.0g; glucose 20.0g; dipotassium hydrogen phosphate 2.0g; triammonium citrate 2.0g; sodium acetate 5.0g; magnesium sulfate 0.2g; manganese sulfate 0.05g; Tween 801.0mL; water 1L.

[0034] Application of the Pediococcus pentosaceae 368 bacterial agent in feed and water according to the invention.

[0035] The effective number of viable bacteria of the thalline in the microbial preparation is 10 10 ~10 11 CFU / g.

[0036] The additive dose in the feed is 10 6 ~10 8 CFU / g, 10 in drinking water 9 ~10 11 CFU / L.

Embodiment 1

[0038] (1) Strain preparation: MRS liquid medium was inoculated with 0.1% inoculum, cultured at 38° C. for 18 hours, and centrifuged at 5000 rpm to obtain bacterial cells. The MRS medium culture liquid is: peptone 10.0g; beef extract 5.0g; yeast powder 4.0g; glucose 20.0g; dipotassium hydrogen phosphate 2.0g; triammonium citrate 2.0g; sodium acetate 5.0g; ; Manganese sulfate 0.05g; Tween 801.0mL; water 1L.

[0039] (2) Freeze-dry the cells.

[0040] The effective number of viable bacteria of the thalline in the microbial preparation is 10 10 ~10 11 CFU / g.

Embodiment 2

[0042] (1) Strain preparation: MRS liquid medium was inoculated with 0.2% inoculum, cultured at 35° C. for 20 h, and centrifuged at 6000 rpm to obtain bacterial cells.

[0043] (2) Freeze-dry the cells.

[0044] The effective number of viable bacteria of the thalline in the microbial preparation is 10 10 ~10 11 CFU / g.

[0045] In step (1), the MRS culture medium is: peptone 10.0g; beef extract 5.0g; yeast powder 4.0g; glucose 20.0g; dipotassium hydrogen phosphate 2.0g; triammonium citrate 2.0g; sodium acetate 5.0g; magnesium sulfate 0.2g; manganese sulfate 0.05g; Tween 801.0mL; water 1L.

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Abstract

The invention discloses a pediococcus pentosaceus 368 microbial agent and an application of the pediococcus pentosaceus 368 microbial agent. The pediococcus pentosaceus 368 microbial agent is characterized by being obtained by a preparation method comprising the following steps: (1) strain preparation: inoculating pediococcus pentosaceus 368 into an MRS liquid culture medium, conducting culturing at 32-38 DEG C for 18-20 hours, and conducting centrifuging at 8000-10000 rpm to obtain thalli; and (2) freeze drying on the thalli. The pediococcus pentosaceus 368 microbial agent can significantly inhibit the growth of streptococcus in the intestinal tract of a pig, has disease resistance, and also can improve the average daily gain and the average daily feed intake of the pig, reduce the feed-to-gain ratio, the diarrhea rate and the constipation rate, reduce the content of ammonium nitrogen and crude fibers in excrement, reduce ammonium nitrogen pollution and improve the utilization of the crude fibers.

Description

[0001] This application is a divisional application with an application date of July 21, 2020, an application number of 202010714586.3, and an invention title of "A Pediococcus Pentosaceae 368 and Its Application". technical field [0002] The invention relates to the field of biotechnology, in particular to a Pediococcus pentosaceae 368 bacterial agent and its preparation method and application. Background technique [0003] Lactic acid bacteria regulate intestinal pH through metabolites, thereby promoting animal growth, adjusting the structure and relative abundance of intestinal flora, thereby improving gastrointestinal function, improving growth performance and reducing morbidity. Pediococcus pentosaceus (Pediococcuspentosaceus) belongs to the genus Pediococcus of the family Streptococcus, can ferment glucose to produce lactic acid, and can inhibit pathogenic bacteria such as Aeromonas hydrophila, Listeria, and Bacillus cereus. However, there is no report on the inhibito...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K50/30A23K10/18A61K35/744A61P31/04A61P1/12A61P1/10C12R1/01
CPCC12N1/20A23K50/30A23K10/18A61K35/744A61P31/04A61P1/12A61P1/10
Inventor 赵彦光刘韶娜赵智勇张斌相德才
Owner YUNNAN ANIMAL SCI & VETERINARY INST
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