The invention discloses a
mulberry leaf fermentation extract as well as a preparation method and application thereof, and belongs to the technical field of
fermentation extract preparation. The preparation method of the
mulberry leaf fermentation extract comprises the following steps: S1,
mincing dry mulberry leaves, putting the minced mulberry leaves into an
ethanol water solution, carrying out ultrasonic extraction, and distilling; adding
distilled water to a constant volume, adjusting pH, and sterilizing at high temperature to obtain
mulberry leaf feed liquid; s2, firstly inoculating fungi into the mulberry leaf feed liquid, performing liquid fermentation treatment for 2-3 days, then inoculating
lactobacillus plantarum and
pediococcus pentosaceus, performing liquid fermentation treatment for 4-5 days to obtain a fermentation product, centrifuging the fermentation product, taking supernatant filtrate, and
drying to obtain the mulberry leaf fermentation extract. Comprising the following
active components: 1-deoxynojirimycin (DNJ) and flavone, the content of 1-deoxynojirimycin is greater than 1.2%, and the content of flavone is greater than 1%; the
bitter taste is greatly reduced, and the product can be applied to hypoglycemic food.