Method for removing heavy metal cadmium from rice by utilizing complex lactobacillus fermentation

A lactic acid bacteria fermentation, heavy metal technology, applied in the application, food preparation, food science and other directions, can solve the problems of reducing the protein and ash content of rice flour, and achieve the effects of high removal rate, significant social and economic benefits, and simple and reliable process

Active Publication Date: 2015-05-06
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

Wang Feng (2003) studied the components of rice flour after soaking and fermentation, and found that the protein and ash content in rice flour were significantly r

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 10 g of rice samples with a cadmium content of 0.6479 mg / kg, crush them through a 40-mesh sieve, add 40 mL of deionized water, and mix to make a rice slurry; and 37°C for 24 hours to activate the strains; put the activated single colonies of slanted strains of Lactobacillus plantarum and Pediococcus pentosaceus into the sterilized MRS broth medium respectively, at 42°C and 37°C respectively Cultivate at constant temperature for 48 hours, respectively prepare Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution; mix Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution at a ratio of 2:1 (v / v) Mix, add 3% (v / v) inoculum into the rice slurry, and ferment at a constant temperature of 40°C for 24 hours; after the fermentation is completed, filter the fermentation liquid to obtain fermented rice flour, which is fermented 3-5 times Wash the fermented rice flour 3 times with deionized water ...

Embodiment 2

[0022] Take 10 g of rice samples with a cadmium content of 0.4783 mg / kg, crush them through a 50-mesh sieve, add 50 mL of deionized water, and mix to make a rice slurry; and 37°C for 24h to activate the strains; the activated single colonies of the slanted strains of Lactobacillus plantarum and Pediococcus pentosacea were respectively inserted into the sterilized MRS broth medium, and the bacteria were incubated at 37°C and 42°C respectively. Cultivate at constant temperature for 24 hours to prepare Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution respectively; the ratio of Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution is 2:1 (v / v) Mix, add 5% (v / v) inoculum into the rice slurry, and ferment at a constant temperature of 43°C for 18 hours; after the fermentation is complete, filter the fermentation liquid to obtain fermented rice flour, which is fermented 3-5 times Wash the fermented ...

Embodiment 3

[0024] Take 10 g of rice samples with a cadmium content of 0.8095 mg / kg, crush them through a 60-mesh sieve, add 60 mL of deionized water, and mix to make a rice slurry; and 37°C for 24h to activate the strains; put the activated single colony of Lactobacillus plantarum and Pediococcus pentosaceus into the sterilized MRS broth medium, respectively, at 40°C and 39°C Cultivate at constant temperature for 36 hours to prepare Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution respectively; mix Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution at a ratio of 2:1 (v / v) Mix, insert into rice slurry according to 4% (v / v) inoculum amount, and ferment at a constant temperature at 37°C for 30 hours; after the fermentation is completed, filter the fermentation liquid to obtain fermented rice flour, which is fermented with 3-5 times Wash the fermented rice flour 4 times with deionized water of the weigh...

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Abstract

The invention relates to a method for removing heavy metal cadmium from rice by utilizing complex lactobacillus fermentation. The method comprises the following steps: crushing a rice sample with the content of cadmium of more than 0.2mg/kg, sieving, and adding deionized water; inoculating a mixed zymocyte suspension containing a lactobacillus plantarum seed culture solution and a pediococcus pentosaceus seed culture solution, standing, fermenting at a constant temperature, washing fermented rice flour with water, and centrifugally dewatering to remove free heavy metal cadmium ions in the rice; and finally, drying centrifuged rice flour wet residues. By adopting the method provided by the invention, the removal rate of the cadmium in the rice is up to 70% above; the dewatered rice flour can be used as a feed or used for producing fermented rice flour after being dried by hot air. The content of the heavy metal cadmium in the rice can be effectively reduced, and the utilization problem of the rice with the excessive cadmium is effectively solved.

Description

technical field [0001] The invention belongs to the technical field of rice deep processing, and in particular relates to a method for removing heavy metal cadmium from rice by fermentation with compound lactic acid bacteria. Background technique [0002] Rice is one of the staple foods of most people in the world, and Chinese residents cannot do without rice for three meals a day. Therefore, the yield and quality of rice are highly valued by Chinese people. [0003] However, in recent years, heavy metal pollution in rice has become a serious problem in China. The Ministry of Agriculture once conducted safety sampling inspections on rice, and the results showed that the most serious heavy metal exceeding the standard was lead, followed by cadmium. Moreover, whether it is the sampling inspection of the Ministry of Agriculture or the investigation and research of scholars, it is shown that about 10% of the rice in the Chinese market has excessive cadmium. After the media exp...

Claims

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Application Information

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IPC IPC(8): A23L1/015A23L1/10A23L5/20A23L7/10
Inventor 吴卫国傅亚平廖卢艳王巨涛
Owner HUNAN AGRICULTURAL UNIV
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