7 results about "Tetragenococcus halophilus" patented technology

The application discloses a phosphoglucomutase, a mutant and application of the phosphoglucomutase and the mutant in improving yield of exocellular polysaccharide of lactic acid bacteria, and relates to the technical field of microbial engineering. The amino acid sequence of the phosphoglucomutase is shown as SEQ ID NO. 1. After the phosphoglucomutase is transferred into a host cell, the yield of exocellular polysaccharide of the lactic acid bacteria can be improved. Further, the amino acid at position 526 of the phosphoglucomutase from tetragenococcus halophilus is mutated, and the phosphoglucomutase mutant with T526I mutation can further improve the yield of exocellular polysaccharide of the lactic acid bacteria.

The invention discloses tetragenococcus halophilus and an application thereof. The preservation number of the tetragenococcus halophilus is GDMCC (China General Microbiological Culture Collection Center) 66036, and the preservation number of the tetragenococcus halophilus is CGMCC 66036. The tetracoccus halophilus is sensitive to eight antibiotics such as ampicillin, gentamicin and kanamycin, does not have drug-resistant genes and virulence genes, and can generate functional active substances with higher levels, such as protocatechuic acid, hydroxyphenyllactic acid, phenyllactic acid, 4-(methylthio)-2-butanol and 3-indoleacrylic acid. The tetracoccus halophilus has high capacity of generating volatile flavor substances and can be used for improving flavor characteristics of the fermented soybean paste, and the fermented soybean paste prepared by fermentation of the tetracoccus halophilus has rich ester fragrance, high sweet and delicious taste and low bitter aftertaste. The fermented soybean paste prepared by fermentation of the tetragenococcus halophilus has a relatively high level of free amino acid content, especially sweet and delicious free amino acids, and also has a relatively high level of succinic acid and malic acid content.

This invention relates to the field of bioengineering fermentation technology, and particularly to a composite anti-browning agent comprising probiotics and bioactive peptides and its application. The composite anti-browning agent comprises *Lactobacillus plantarum* (…). Lactiplantibacillus plantarum ), Halophilic tetrad cocci ( Tetragenococcus halophilus The addition of active peptides can give soy sauce products a rich umami and salty flavor, and can also enhance color, inhibit browning, and give it a bright reddish-brown color. At the same time, it can comprehensively improve the quality of fermented soy sauce and the utilization rate of raw materials, and promote the development of the soy sauce industry towards safety, quality and efficiency.

The application discloses a kind of functional composite microbial inoculant of reducing alkali and desalination and preparation method thereof, related to saline-alkali soil improvement and agricultural microbial inoculant field, including composite functional bacteria powder, composite modified carrier and composite protective agent, by weight fraction, the composite functional bacteria powder 5-15 parts, composite modified carrier 75-90 parts, composite protective agent 3-10 parts, the composite functional bacteria powder is by Bacillus subtilis powder, Bacillus mucilaginosus powder, Haloterrigena quadriceps powder, Pseudomonas stutzeri powder and Rhodospirillum rubrum powder compound, the composite modified carrier is by modified corn stalk biochar, humic acid potassium, modified diatomite compound, by screening suitable salt-tolerant functional strain combination, no antagonism between each strain, function complementary synergy, can realize the multiple effect of soil alkalinity neutralization, soluble salt degradation, soil nutrient activation, crop growth promotion simultaneously, compared with prior art, improve the comprehensive improvement efficiency of saline-alkali soil.

The invention relates to the technical field of microorganisms. Specifically, the invention relates to tetracoccus halophilus with the characteristic of low acetic acid yield, which can reduce the content of acetic acid, ensure the content of lactic acid in soy sauce mash, further promote the alcoholic fermentation of soy sauce yeast and contribute to greatly increasing the content of characteristic flavor substances in soy sauce. The invention also relates to a method for preparing soy sauce or sauce. The method comprises the step of adding the tetracoccus halophilus disclosed by the invention before soy sauce mash fermentation or in the soy sauce mash fermentation process.

Provided are a tetragenococcus halophilus with an inhibitory effect on bacillus cereus and a use thereof. The preservation number of the tetragenococcus halophilus is GDMCC 64945. The tetragenococcus halophilus is isolated from fermented bean curd, and colonies of the tetragenococcus halophilus are milky white, small and round on a solid medium; The tetragenococcus halophilus is Gram-positive, and when observed under a microscope, the morphology thereof is mostly diplococci or tetrads, with no chain-like formations. The strain is sensitive to eight antibiotics such as ampicillin, gentamicin and kanamycin, and has no drug resistance gene and virulence gene. A bacterial suspension of the strain has a good inhibitory effect on a bacillus cereus strain isolated from the fermented bean curd, and when the strain is applied to a fermented bean curd fermentation system, the growth of bacillus cereus in the system can be effectively inhibited, and the content of enterotoxin in the system is reduced.

A new strain of Tessaracoccus for promoting the growth of black soldier fly larvae and its application are provided, the strain comprises a 16S rDNA sequence shown in SEQ ID NO: 1 or its complement or a sequence having at least 85% nucleic acid identity compared with SEQ ID NO: 1. The Tessaracoccus sp. Tess.M30 disclosed in the application has the characteristics of decomposing starch and salt tolerance. Adding the functional bacteria in the process of black soldier fly breeding can promote the growth of larvae and promote the accumulation of protein and fat in larvae. Compared with adding sterile water, after adding Tess.M30, the body length and body weight of black soldier fly increase by 10.82% and 48.80% respectively, and the accumulation of protein and fat increases by 61.65% and 76.80% respectively. The application of the technology can improve the biological conversion efficiency of black soldier fly, and is conducive to promoting the industrialized production of black soldier fly larvae.