Complex microbial inoculum applied in high-salinity organic wastewater and preparation method of complex microbial inoculum

A technology of organic wastewater and compound bacterial agents, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as toxicity, secondary pollution, and difficult application

Pending Publication Date: 2019-08-23
新疆水处理工程技术研究中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For these high-salinity refractory industrial wastewater, it is best to be treated separately in the sewage treatment station in industrial enterprises. If these wastewater is mixed or diluted with other industrial wastewater or domestic sewage containing easily biodegradable substrates, and discharged into municipal pipes, Microorganisms in urban sewage treatment plants preferentially metabolize the latter, and these toxic pollutants will be difficult to

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The preparation method of various strains of aseptic liquid medium is as follows:

[0028] Seacoccus: yeast extract 4.0g, malt extract 10.0g, glucose 4.0g, sodium chloride 15.0g, distilled water 1.0L, adjust pH to 7.3 with 1% hydrochloric acid and 10% sodium hydroxide, autoclave at 121°C for 15min .

[0029] Tetradococcus halophilus: 6.0g sodium chloride, 0.1g magnesium sulfate heptahydrate, 0.5g potassium dihydrogen phosphate, 0.1g calcium chloride, 2.0g dipotassium hydrogen phosphate, 2.0g ammonium sulfate, 1.0g yeast extract, , 1.0L of distilled water, adjust the pH to 7.0±0.2 with 1% hydrochloric acid and 10% sodium hydroxide, and autoclave at 121°C for 15 minutes.

[0030] Konjacella: 24.0g sodium chloride, 1.0g ammonium nitrate, 0.7g potassium chloride, 2.0g potassium dihydrogen phosphate, 3.0g sodium dihydrogen phosphate, 7.0g magnesium sulfate heptahydrate, 1.0L distilled water, trace element formula: , calcium chloride 0.02mg, ferric chloride 0.5mg, copper su...

Embodiment 1

[0034] Step 1: inoculate seaococcus, halophilic tetrad, konjac, sulfite bacillus, and sphaericus into respective Erlenmeyer flasks containing sterile liquid medium; adjust the pH value of the medium to 7.0 , and then cultured on a shaker at 32° C. and 120 r / min for 24 hours to obtain the activated culture solution of each bacteria.

[0035] Step 2: The activated culture solution is respectively connected to each fermenter according to the inoculation amount of 10% of the volume of each fermenter. The liquid medium used in each fermenter is the same as step 1. The state of the fermenter is 32°C, 180r / min, and sterile air is introduced. , the ventilation rate is 1.2 times / min of the volume of the fermenter, fermented to 1.0-3.0×10 sea cocci 8 cfu / g, tetradococcus halophilus 1.0-3.0×10 9 cfu / g, Konjacella 1.0-3.0×10 8 cfu / g, sulfite bacteria 1.0-3.0×10 9 cfu / g, Bacillus sphaericus 1.0-3.0×10 10 After cfu / g, the fermentation was stopped, and seawater coccus, halophilic tetrad,...

Embodiment 2

[0039] Step 1: inoculate seaococcus, halophilic tetrad, konjac, sulfite bacillus, and sphaericus into respective Erlenmeyer flasks containing sterile liquid medium; adjust the pH value of the medium to 7.0 ±0.2, and then cultured on a shaker at 32°C and 120r / min for 24 hours to obtain the activated culture solution of each bacteria.

[0040] Step 2: The activated culture solution is respectively connected to each fermenter according to the inoculation amount of 10% of the volume of each fermenter. The liquid medium used in each fermenter is the same as step 1. The state of the fermenter is 32°C, 180r / min, and sterile air is introduced. , the ventilation rate is 1.2 times / min of the volume of the fermenter, fermented to 3.0-5.0×10 8 cfu / g, tetradococcus halophilus 3.0-5.0×10 9 cfu / g, Konjacella 3.0-5.0×10 8 cfu / g, Bacillus sulfite 3.0-5.0×10 9 cfu / g, Bacillus sphaericus 3.0-5.0×10 10 After cfu / g, the fermentation was stopped, and seawater coccus, halophilic tetrad, Kangeria, ...

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Abstract

The invention discloses a complex microbial inoculum applied in high-salinity organic wastewater. The complex microbial inoculum is prepared from, by mass, 10-25% of salinicoccus sp, 10-25% of tetragenococcus halophilus, 10-25% of kangiella sp, 5-25% of sulfitobacter sp and 10-25% of bacillus sphaericus. The invention further discloses a preparation method of the complex microbial inoculum. The complex microbial inoculum can stably grow in the high-salinity environment, utilizes the metabolism of bacteria themselves for treatment, is green and environmentally friendly, and cannot cause secondary pollution to the environment, after the complex functional microbial inoculum is added, microorganisms can steadily increase, and after one week of stable operation, it is measured that the COD content is reduced from 869 mg/L to 38-52 mg/L; the ammonia nitrogen is reduced from original 68 mg/L to 1.08-1.65 mg/L, the COD content and ammonia nitrogen content in the high-salinity organic wastewater can be effectively reduced, the removal rate of COD in high-salinity printing and dyeing wastewater can reach 94% or above, and the removal rate of the ammonia nitrogen can reach 97% or above.

Description

technical field [0001] The invention relates to the technical field of wastewater treatment, in particular to a composite bacterial agent applied to high-salt organic wastewater and a preparation method thereof. Background technique [0002] The discharge of saline wastewater has caused very serious environmental pollution. Saline wastewater is widely sourced from industrial production. In addition to being limited by its own high concentration of salt, it also contains a large amount of toxic and refractory organic matter. For these high-salinity refractory industrial wastewater, it is best to be treated separately in the sewage treatment station in industrial enterprises. If these wastewater is mixed or diluted with other industrial wastewater or domestic sewage containing easily biodegradable substrates, and discharged into municipal pipes, Microorganisms in urban sewage treatment plants preferentially metabolize the latter, and these toxic pollutants will be difficult to...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F101/30C12R1/07C12R1/01
CPCC12N1/20C02F3/34C02F2101/30
Inventor 宋勇张如来周镇江许白羽
Owner 新疆水处理工程技术研究中心有限公司
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