93 results about "Bacillus sphaericus" patented technology

The invention relates to a bioflocculant fermentation method, in particular to a bioflocculant fermentation method with a mycelium pellet as a vector. The method solves the problems of high energy consumption, high production cost and unsuitability for large-scale industrialized fermentation production and the like existing in the existing bioflocculant fermentation method and comprises the following steps of: 1, mixing rhizobium radiobacter with bacillus sphaericus and then culturing so as to obtain flocculant-producing bacteria seed liquid; 2, culturing aspergillus niger to be a mature mycelium pellet; 3, mixing the mature mycelium pellet with the flocculant-producing bacteria seed liquid and then culturing so as to obtain a mixed mycelium pellet; 4, taking 24-hour as a fermentation period, and drawing out the mixed mycelium pellet after each fermentation period is finished and pouring the mixed mycelium pellet into fresh culture mediums for culturing; and 5, repetitively operating the step 4 for 30-35 times and namely completing. According to the bioflocculant fermentation method provided by the invention, on the premise of guaranteeing stable flocculating rate, seed liquid demand is reduced, production efficiency is increased and production energy consumption is reduced; moreover, the invention has great significance on large-scale industrialized production of bioflocculant.

The invention relates to a liquid composite biological multi-control fertilizer. The invention is characterized in that the liquid composite biological multi-control fertilizer is prepared from the following raw materials in parts by weight: 1-3 parts of Streptomyces hygroscopicus solution, 1-3 parts of Streptomyces cinnamofuscus solution, 2-4 parts of Bacillus amyloliquefaciens solution, 2-4 parts of bacillus sphaericus solution, 7-15 parts of Bacillus mucilaginosus solution, 1-3 parts of Azotobacter chroococcum solution, 1-3 parts of Streptomyces aureochrogmoenes, 8-10 parts of Trichoderma viride fermentative degradation solution, 8-10 parts of matrix and 45-65 parts of sterilized water. The invention prepares a crop-absorbing carbohydrate and organic matter Trichoderma viride fermentative degradation solution, and provides high-efficiency beneficial microbial florae with phosphate solubilizing, potassium solubilizing, nitrogen fixation and disease resistance effects. The fertilizer can simultaneously perform the three functions of biological pesticide, biofertilizer and biological nutrients, and has the advantages of no toxicity or harm and no environment pollution. Compared with other fertilizers with the same nutrients, the crop yield is increased by 20%.

The invention belongs to the field of biological prevention and control on crop insect damage and provides a microbial preparation for inhibiting root-knot nematode. The preparation is formed by mixing bacterial liquids of streptomyces avermitilis, paecilomyces lilacinus, verticillium chlamydosporium and Bacillus sphaericus and glycerol. The invention also provides a production method and an application effect of the microbial preparation for inhibiting the root-knot nematode. The microbial preparation comprises viable bacteria of which the number is greater than 2,500 millions per milliliter, wherein the viable bacteria comprise the streptomyces avermitilis, the paecilomyces lilacinus, the verticillium chlamydosporium and the Bacillus sphaericus. After the microbial preparation is prepared into soil, eggs of the root-knot nematode are killed by metabolites generated in the continuous growth and reproduction activity process of the viable bacteria, so that a granular structure of the soil is improved, the utilization rate of a fertilizer is improved and an effect of increasing both production and income is achieved.

The invention discloses an environment-friendly composite microorganism preparation and a preparation method thereof, and belongs to the technical field of environmental biology; the composite microorganism preparation of the invention comprises the following components: (1) a composite microbial agent accounting for 10% of the total weight and comprising sporobolomyces, bacillus sphaericus, lactobacillus plantarum and rhodobacter sphaeroides; (2) a microorganism carrier accounting for 90% of the total weight and comprising 20-50 parts of wheat bran or rice bran, 160-300 parts of organic bentonite, and 40-80 parts of water. The invention compounds bacteria and fungi, enlarges the application scope, and is applicable to not only deodorization treatment of municipal solid household garbage, production sewage, and the like, but also degradation of waste water polluted by heavy metal chromium.

Disclosed is an antibacterial treatment method which has no limitation in the scope of a material to be treated and which enables to reduce the time or cost required for the antibacterial testament. Specifically disclosed is an antibacterial treatment method using an antibacterial agent comprising a powder produced by mixing a microorganism selected from Bacillus sphaericus, Bacillus subtilis and Bacillus thuringiensis with a cattle feces which is previously treated at a high temperature of 60 to 150° C., wherein the material to be treated and the antibacterial agent are arranged under a non-contacting condition to inhibit the growth of a target bacterium in the material.

A cement paste containing microbial cells which has a sustained effect and an excellent function of purifying water. This cement paste contains a mixture of Bacillus subtilis, Bacillus thuringiensis and Bacillus sphaericus cells at a mixing ratio by mass of 0.1 to 50:0.1 to 50:0.1 to 50.

The invention relates to a biological organic fertilizer used for aquaculture paddy fields and the production method thereof. The fertilizer is prepared by the raw materials according to the parts by weight: 0.3 to 0.5 parts of taurine, 2 to 3 parts of treptomyces hygroscopicus, 2 to 3 parts of chromagenic streptomyces aureofaciens, 2 to 4 parts of bacillus cereus, 2 to 4 parts of bacillus sphaericus, 9 to 13 parts of bacillus mucilaginosus, 2 to 3 parts of azotobacter chroococcum, 2 to 3 parts of streptomyces microflavus, 10 parts of fruit waste degradation products, 10 parts of matrix and 46.5 to 57 parts of soil. In the method, as fruit waste degradation product polysaccharide carbohydrate is mixed with nitrogen-fixing, phosphate-dissolving and potassium-dissolving active compound microorganisms and a compound actinomycete agent which has antagonistic effect on rice blast and rice sheath blight, and matched with nutritional components which stimulate the growth of fisheries in rice fields, the biological organic fertilizer can not only be used for improving soil microbial community structure, activating soil nutrients, and improving rice rhizosphere nutrition, increasing the yield and quality of rice and improving water quality, but also promote the growth of cultured fisheries in rice fields.

The invention discloses an environment-friendly organic biological compound fertilizer comprising the following substances in parts by weight: 35-45 parts of chicken manure, 10-15 parts of potato powder, 20-35 parts of peat, 9-16 parts of calcium superphosphate, 7-10 parts of potassium sulphate, 1-6 parts of calcium nitrate, 2-6 parts of magnesium sulfate, 2-6 parts of sodium chloride, 0.1-0.2 part of bacillus sphaericus powder, 35-45 parts of rice straw, 30-40 parts of corn straw, 0.1-0.2 part of abamectin mixture, 1-6 parts of lotus root starch, 2-6 parts of monopotassium phosphate, 1-2 parts of compound sodium nitrophenolate, 1-2 parts of 1-naphthylacetic acid and 1-2 parts of humic acid. The environment-friendly organic biological compound fertilizer does not contain a great number of nutrient elements; and after being used, the environment-friendly organic biological compound fertilizer can provide various nutritional ingredients for crops and a great deal of biotin amino acids, vitamins, growth hormones, antibiotics and other various bioactive substances for the crops through the key roles of microorganisms contained in the environment-friendly organic biological compound fertilizer.

The invention relates to a microbial fertilizer. The microbial fertilizer comprises 84-90% by mass of organic matters and 5-11% by mass of water and also comprises a strain, wherein the strain comprises at least two kinds of aerobic bacteria and at least one anaerobic bacterium; each aerobic bacterium is any one of bacillus subtilis, bacillus megaterium, bacillus stearothermophilus, bacillus sphaericus, bacillus firmus and saccharomycetes; the anaerobic bacterium is any one of bacillus circulans, bacillus licheniformis and lactobacillus acidophilus. The microbial fertilizer has the beneficial effects that the microbial fertilizer has the effects of overcoming the defects of the existing chemical fertilizers, reducing environmental pollution and activating soil; the content of beneficial biological bacteria in the microbial fertilizer is more than 0.3billion / g and biological activating enzymes and various trace elements are supplied, so that the quality of crops has a qualitative leap; through simultaneously containing the aerobic bacteria and the anaerobic bacteria, the microbial fertilizer can work under different conditions, thus increasing the output rates of the crops.

It is intended to provide an agent for treating fungi which is capable of controlling the production cost while using a microorganism with high safety, and also maintaining an adhesion state on a surface of a target for a predetermined period of time or longer during use, and a method for treating fungi. The agent for treating fungi is characterized by containing a microorganism-containing liquid obtained by adding water to powder prepared by mixing a Bacillus sphaericus microorganism, a Bacillus subtilis microorganism, or a Bacillus thuringiensis microorganism with livestock manure treated at a high temperature between 60° C. and 150° C., a surfactant, and a propellant. The agent for treating fungi is characterized in that, preferably, the surfactant is at least one type selected from the group consisting of sodium lauryl sulfate, polyoxyethylene cetyl ether, and potassium fatty acid, and the propellant is LP gas.

Slow-release fertilizer is produced by composting animal manure with a magnesium-rich compound The temperature of this mixture is maintained at 20-30° C. and the pH is maintained between 7-10. Urease is added to the mixture. Next, the mixture is inoculated with bacteria of the species Bacillus sphaericus, Bacillus globisporus, or Bacillus fusiformis. The mixture is then allowed to incubate for about 14 days to form magnesium ammonium phosphate. Optionally, phosphatase, an enzyme promoting the formation of phosphate from phosphorus-rich organic compounds, is added with the urease enzyme to increase the yield of magnesium-ammonium phosphate.

The invention relates to the field of biologically-derived insecticidal preparations, in particular to a complex microbial inoculant for controlling root-knot nematodes of crops and a preparation method of the complex microbial inoculant. The complex microbial inoculant for controlling the root-knot nematodes of the crops and the preparation method of the complex microbial inoculant are characterized in that microorganism is composed of bacillus subtilis, bacillus sphaericus, pseudomonas fluorescens, bacillus thuringiensis, brevibacillus laterosporus, paenibacillus polymyxa, pasteurella pneumoniae, paecilomyces lilacinus, verticillium chalamydosporium, beauveria bassiana and aspergillus fumigatus. The novel multi-bacteria same-pot fermentation technology is adopted, the formula is clever and reasonable, the disadvantages of mutual antagonism in the liquid deep fermentation culture of compound bacteria are eliminated, the compound bacteria has high activity, and the complex microbial inoculant for controlling the root-knot nematodes of the crops has a good control effect on tomatoes and cucumber root-knot nematodes.

The invention provides a mosquito killing Bacillus sphaericus leafing agent which is prepared by the following steps: firstly, concentrating mosquito killing Bacillus sphaericus ferment filtrate by a film concentration method; then, adsorbing the mosquito killing Bacillus sphaericus ferment filtrate by a porous carrier substance, mixing the mosquito killing Bacillus sphaericus ferment filtrate and sustained release agent and drying the mosquito killing Bacillus sphaericus ferment filtrate into a master batch at 40 DEG C-50DEG C; and finally, coating hydrophobing agent. The mosquito killing Bacillus sphaericus leafing agent is prepared by a low temperature production process, the loss rate (namely valence loss rate) of active components is only about 10 percent, and the solid recovery rate reaches about 95 percent. The technology ensures low valence loss rate, low energy consumption and low transportation cost in the production process. By measurement, the leafing agent has the effect on continuously killing Culex fatigans for more than 28 days (indoor measurement) by putting the 4mg / L leafing agent into water at one time.

A method for controlling Dipteran larvae or a method for inhibiting the development of larvicidal resistance, controlling resistant populations and reducing resistance levels in Diptera by introducing a larvicidally-effective amount of a combination of a strain of Bacillus thuringiensis subspecies israelensis and a strain of Bacillus sphaericus into an environment containing Dipteran larvae; and a composition of the combination are disclosed. Preferably both strains are non-genetically modified.

A biopesticide for herbaceous plants includes the following components, by weight: 0.8-1.2 parts of bacillus sphaericus, 0.6-0.9 parts of bacillus thuringiensis, 2-6 parts of metarhizium anisopliae, 4-7 parts of paecilomyces lilacinus, 3-7 parts of avermectin, 4-8 parts of ivermectin, 5-9 parts of amino oligosaccharin, 4-8 parts of sophocarpidine, 3-7 parts of veratrine, 6-11 parts of osthole, 0.9-1.6 parts of cis-9-tricosene, 57-65 parts of a synergist, and 12-19 parts of 8% ethanol, and preferably includes, by weight, 1 part of the bacillus sphaericus, 0.7 parts of the bacillus thuringiensis, 4 parts of the metarhizium anisopliae, 6 parts of the paecilomyces lilacinus, 5 parts of the avermectin, 6 parts of the ivermectin, 7 parts of the amino oligosaccharin, 6 parts of the sophocarpidine, 5 parts of the veratrine, 8 parts of the osthole, 1.2 parts of the cis-9-tricosene, 61 parts of the synergist, and 16 parts of the 8% ethanol. The biopesticide is excellent in effects, and is free of toxic and side effects, pesticide residue and ecologic and environmental pollution.

A biologically pure culture of Bacillus sphaericus strain 205y capable of producing an organic solvent-tolerant lipase was isolated from soil samples via direct plating method using 1% (v / v) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source, ethyl benzene and ρ-xylene. The lipase gene was isolated via genomic library and sequenced. The production of lipase gene from Bacillus sphaericus strain 205y is a novel gene. The lipase gene was cloned from Bacillus sphaericus strain 205y and the recombinant lipase was efficiently excreted into the culture medium using expression vectors bearing the lipase gene.

The invention discloses a bacillus sphaericus with high yield and high-temperature xylanase resistance and an application thereof. The bacillus sphaericus is classified and named as Lysinibacillus sphaericus Xyn-1, and is collected in China Center for Type Culture Collection (CCTCC) on May, 5th, 2014 with the collection number CCTCCM2014183. The optimal fermentation enzyme production condition of a strain screening shake flask is determined by adopting a Plackett-Burman design and a response surface analysis method, and the fermentation level under the condition can reach 5678.5IU / mL. When the enzyme produced by fermentation of the strain is applied to degradation of plant hemicellulose, the xylobiose yield of the hemicellulose can reach over 60% through enzymolysis.

The invention discloses a bacillus sphaericus DS 11. The strain is a short rod shape Gram-positive bacteria, the growth temperature range of the strain is 4 to 35 DEG C and the optimal growth temperature is 28 DEG C; the growth pH range is between 4 and 11, and the optimal growth pH is 7; the growth NaCl concentration is between 0.3 and 10 percent, and the optimal NaCl concentration is 3 percent. The bacillus sphaericus DS 11 can endure an organic solvent with Log P of less than or equal to 2.0. The invention further discloses a method for producing an inactive protease of the organic solvent by utilizing the bacillus sphaericus DS 11 as well as the inactive protease product of the organic solvent which is obtained by the method, has stronger organic solvent tolerance and can be used for the synthesis of organic-phase high-performance catalytic polypeptide.

A method for controlling Dipteran larvae or a method for inhibiting the development of larvicidal resistance, controlling resistant populations and reducing resistance levels in Diptera by introducing a larvicidally-effective amount of a combination of a strain of Bacillus thuringiensis subspecies israelensis and a strain of Bacillus sphaericus into an environment containing Dipteran larvae; and a composition of the combination are disclosed. Preferably both strains are non-genetically modified.

A cement paste containing microbial cells which has a sustained effect and an excellent function of purifying water. This cement paste contains a mixture of Bacillus subtilis, Bacillus thuringiensis and Bacillus sphaericus cells at a mixing ratio by mass of 0.1 to 50:0.1 to 50:0.1 to 50.

The invention discloses a complex microbial inoculum applied in high-salinity organic wastewater. The complex microbial inoculum is prepared from, by mass, 10-25% of salinicoccus sp, 10-25% of tetragenococcus halophilus, 10-25% of kangiella sp, 5-25% of sulfitobacter sp and 10-25% of bacillus sphaericus. The invention further discloses a preparation method of the complex microbial inoculum. The complex microbial inoculum can stably grow in the high-salinity environment, utilizes the metabolism of bacteria themselves for treatment, is green and environmentally friendly, and cannot cause secondary pollution to the environment, after the complex functional microbial inoculum is added, microorganisms can steadily increase, and after one week of stable operation, it is measured that the COD content is reduced from 869 mg / L to 38-52 mg / L; the ammonia nitrogen is reduced from original 68 mg / L to 1.08-1.65 mg / L, the COD content and ammonia nitrogen content in the high-salinity organic wastewater can be effectively reduced, the removal rate of COD in high-salinity printing and dyeing wastewater can reach 94% or above, and the removal rate of the ammonia nitrogen can reach 97% or above.

The invention discloses a strain composition suitable for fermenting needle mushroom residues and brewer's grains, and application thereof. The fermentation strain composition comprises Bacillus bacillus (P.bovis CGMCC 8333) and Lactobacillus plantarum ST-III (CGMCC 0847), or Bacillus sphaericus, Lactobacillus plantarum ST-III and Bacillus polymyxa (P.polymyxa CGMCC 10062). The fermentation strain composition provided by the invention cleverly combines several bacteria together so that the fermentation strain composition can ferment the brewer's grains and needle mushroom residues. The product obtained after fermentation smells mellow, and has strong palatability, and the dry matter of the product obtained after fermentation has increased protein content and is easily absorbed by animals; in addition, the strain composition also has the advantages of low crude fiber content, reduction in degree of mildew and the like, and is suitable for being promoted and applied in the large and medium-sized pasture.

The invention discloses a method for preparing and regenerating a lysine bacillus protoplast capable of degrading MC-LR and relates to the technical field of methods for preparing and regenerating protoplasts in bioengineering. According to the method, the protoplast fusion technology for establishing engineering bacteria is taken as the basis, and the lysine bacillus protoplast is prepared from the separated MC-LR high-efficiency degrading bacteria T1(Bacillus sphaericus) protoplast. According to the research, an inactivated protoplast fusion method is selected, so that the workload is reduced, and the quality of a fusant in the subsequent research is guaranteed. The method for preparing and regenerating the protoplast is simple in process, convenient to operate and high in repeatability; and the obtained protoplast preparation rate can be up to 96.64 percent, the regeneration rate can be 83.95 percent, the protoplast is high in preparation rate and high in activity, and the preparation and regeneration of the subsequent fusant are facilitated.

The invention provides a compound continuous-cropping-resistant biological fungicide special for ginsengs / Chinese yams / peanuts and a preparation method thereof. The compound continuous-cropping-resistant biological fungicide is prepared from sheep manure, short and small bacillus pumilus, bacillus amyloliquefaciens liquid, bacillus sphaericus liquid, bacillus licheniformis liquid, paecilomyces fumosoroseus liquid, penicillium oxalicum liquid, streptomyces hygroscopicus liquid, streptomyces griseochromogenes liquid and an auxiliary matrix. The preparation method comprises the steps that 1, the bacterial liquids are prepared respectively and are mixed evenly; 2, main and auxiliary matrixes are evenly mixed, and then drying is performed under the pressure of 30-50 DEG C. The biological fungicide is resistant to continuous cropping, fixes nitrogen, dissolves phosphorus, releases potassium, absorbs moisture, keeps soil humidity, promotes decomposition of soil organic matter, is bacteriostatic and insect-resistant, improves micropopulation in soil, improves the functions of a soil structure, does not pollute the environment and is free of toxicity and residues and safe to human.

The Staphylococcus xylous 2J1 is prepared by separating out from active sludge in sewage disposal tank of petrochemical plant, purifying and culturing. The preservative number is CGMCC No. 1486. It has excellent efficient degradable performance for dimethyl benzene and can be used to dispose waste water and waste gas containing dimethyl benzene, polluted water and soil.

The invention belongs to the field of bio-genetic engineering and provides a shuttle plasmid and a method for efficiently expressing cecropin and lysozyme genes. The invention constructs an escherichia coli-bacillus subtilis shuttle plasmid suitable for being expressed in prokaryotes (escherichia coli, bacillus subtilis and bacillus sphaericus). Cecropins expressed in the prokaryotes does not have a toxic action on hosts. The shuttle plasmid can successfully express the fusion protein of various cecropins such as human lysozyme, bovine lactoferrin, and the like. 6His is introduced into the expression plasmid constructed by the invention, which is convenient to purify, and the purified fusion protein identifies and correctly cut enzyme cutting sites among all fusion peptide segments through lysostaphin so as to release all functional peptides and enable the functional peptides to recover the activity; and moreover, enzyme separation and cutting are also saved, thus the method can be effectively, simply and conveniently used. The shuttle plasmid also has a certain common availability, and all suitable genes can be cloned and expressed.

A water-free process for converting initially non-larvicidal evaporation suppressant powder into a larvicidal variant possessing utility both for abatement of use by mosquitoes of an open water catchment as a successful breeding site, and for conserving water by suppressing the evaporation of water from the same catchment. Lauryl alcohol is used as a blending agent to facilitate even distribution of mosquito-specific entomopathogens, eg., spores of Bacillus thuringiensis variety Israelensis or Bacillus sphaericus, into the initially non-larvicidal evaporation suppressant powder. Cetyl alcohol and / or stearyl alcohol are the preferred monolayer-formers upon which effective evaporation suppression relies. For a variant larvicidal powder that lacks an effective evaporation suppression property, the same preferred monolayer-formers may be omitted from a blend of mosquito-specific pathogens, lauryl alcohol, and hydrated lime or, alternatively, acidified gypsum.

The invention discloses a composite leavening agent for organic material composting. The composite leavening agent comprises the following raw materials in parts by weight: 1-1.5 parts of a mixed microbial agent and 0.5-1 part of a combined enzyme agent, wherein the mixed microbial agent is a mixture of bacillus cereus, bacillus subtilis, bacillus licheniformis, bacillus megatherium, bacillus circulans, bacillus sphaericus, trichoderma viride, trichoderma reesei and aspergillus niger, and the combined enzyme agent is a mixture of protease, cellulase, glucanase and pectinase. The composite leavening agent can quickly start fermentation, prolong the high-temperature period of compost and accelerate the decomposition speed of organic fertilizer, so the quality of the fertilizer is improved, production cost is reduced, and the problem of pollution of organic wastes is solved.

The invention discloses a low-cost biological pesticide. The low-cost biological pesticide comprises, by weight, 5-17 parts of azadirachtin emulsion, 3-9 parts of N-methylpyrrolidone, 11-13 parts of molecular sieve, 25-40 parts of ethanol, 9-13 parts of Fructus Trichosanthis, 25-31 parts of soybean lecithin, 44-54 parts of Bacillus sphaericus, 12-34 parts of Caulis Erycibes, 23-31 parts of a double-component, 11-18 parts of methyl tert-butyl ether, 85-96 parts of bottom oil obtained after production of biodiesel and 25-34 parts of polypropylene glycol. Wastes are processed, and are used as a cheap and effective biological pesticide.

The invention discloses preparation and an application method of a special microorganism composite bacterial agent for directly decomposing and fermenting crop straws to generate marsh gas. The special microorganism composite bacterial agent is liquid or solid, and is formed by fermenting, culturing, combining and compositing of one or more of bacillus cereus, bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus mucilaginosus, bacillus circulans, bacillus sphaericus, clostridium sporogenes, clostridium acetobutylicum, clostridium pasteurianum, clostridium beijerinckii, clostridium thermocellum, trichoderma viride, trichoderma pseudokoningii and aspergillus niger. Meanwhile, by taking the special microorganism composite bacterial agent as an inoculation bacterial agent,the invention provides the application method for decomposing the crop straws and accelerating the fermentation to generate marsh gas; the crop straws treated by the microorganism composite bacterialagent have fast fermentation speed, large marsh gas generation quantity, low treating cost and no secondary pollution, can be fermented to generate the marsh gas under the condition of no any faeces of poultry and livestock, and the application range is quite wide.