31 results about "Bacillus altitudinis" patented technology

The invention relates to a composite flora for treating benzotriazole wastewater. According to the present invention, a strain composition is provided, and the strain composition comprises the following strains: (a) Bacillus altitudinis SD1 having a preservation number of CGMCC 37186, (b) Aquammicrobe defluvii SD4 having a preservation number of CGMCC 37187, and (c) Pseudomonas aeruginosa SD32 having a preservation number of CGMCC 37189, and the strain composition comprises the following strains: (a) Bacillus altitudinis SD1 having a preservation number of CGMCC 37186, (b) Aquammicrobe defluvii SD4 having a preservation number of CGMCC 37187, and (c) Pseudomonas aeruginosa SD32 having a preservation number of CGMCC 37189.

The invention provides application of bacillus altitudinis H18 and a glucoside hydrolase gene thereof in improving rumen degradation of rice straws, and belongs to the technical field of straw reutilization. The bacillus altitudinis H18 is separated from goat rumen fluid, the bacillus altitudinis H18 can promote degradation of rice straw cellulose, and reference is provided for feed application of rice straw. Sequencing analysis finds that a whole genome of the strain H18 contains a large number of genes related to glucoside hydrolase GH, carbohydrate hydrolase CE and the like, and the content of neutral detergent fibers and lignin in the rice straw can be remarkably reduced by performing fermentation treatment on the rice straw by using the strain H18 through an in-vitro fermentation method. The strain can be used as a bacterial strain for feed application of the rice straws so as to improve the conversion rate and the utilization rate of the rice straws.

The invention provides bacillus altitudinis CL6 with a morchella esculenta continuous cropping resisting effect and application of the bacillus altitudinis CL6, and belongs to the technical field of microorganisms. The bacillus altitudinis CL6 with a continuous cropping resisting effect on morchella is separated from morchella rhizosphere soil and is preserved in the China Center for Type Culture Collection on March 18, 2025, and the preservation number is CCTCC NO: M 2025516. The bacillus altitudinis CL6 disclosed by the invention can be used as an auxiliary added beneficial microbial agent in the cultivation production of the morchella to resist the continuous cropping harm of the morchella, and finally, the cultivation stability of the morchella is improved. The strain provided by the invention can promote the continuous cropping resistance effect of morchella esculenta and increase the yield of morchella esculenta. And a green and efficient anti-continuous cropping biological solution is provided for the morchella esculenta cultivation industry.

The invention provides bacillus altitudinis JZBQ5 and application of the bacillus altitudinis JZBQ5 in plant disease prevention and growth promotion. The preservation number of the bacillus altitudinis JZBQ5 is CGMCC (China General Microbiological Culture Collection Center) The bacillus altitudinis JZBQ5 can improve the disease resistance of strawberries, and has a good control effect on powdery mildew and anthracnose of strawberries. Meanwhile, growth of cucumbers and wheat is promoted, and plant diseases such as cucumber root rot and wheat stem rot are effectively prevented and controlled. The strain provides a new strain resource for biological control of crop diseases such as strawberry powdery mildew, and a theoretical basis is provided for a safe and efficient green biocontrol preparation.

The invention relates to the technical field of biopesticides, in particular to a mixed biocontrol microbial inoculum for preventing and treating blueberry gray mold and application of the mixed biocontrol microbial inoculum. The invention relates to a mixed biocontrol microbial agent for preventing and treating blueberry gray mold. The mixed biocontrol microbial agent is prepared from the following components: pseudomonas chlororaphis A4, bacillus altitudinis LY-3, bacillus altitudinis Y-14 and bacillus altitudinis An-2. The four bacteria are mixed and prepared, the four bacteria have an inhibiting effect on the blueberry botrytis cinerea when being independently used, and the four bacteria are mixed and prepared to cooperate with one another to inhibit growth of botrytis cinerea hyphae; the four components are used together, so that gray mold of blueberry fruits in the field can be effectively prevented and treated, and the yield of blueberries is increased.

The application belongs to the technical field of microorganisms, and particularly relates to a bacillus altitudinis BW65 strain against pear white line feather disease and application of the bacillus altitudinis BW65 strain in disease resistance, yield increase and quality improvement. Bacillus altitudinis The strain is isolated from the rhizosphere soil of a peach tree and is classified and named as the BW65 strain. The strain has the abilities of IAA production, nitrogen fixation, organic phosphorus and inorganic phosphorus decomposition, can significantly promote the growth of pear, tomato and other plants, improve the root morphology, increase the biomass and fruit quality, and has antagonistic effects on multiple pathogenic bacteria such as rice blast, alternaria alternata, fusarium graminearum and botryosphaeria dothidea. Application of the bacterial agent can improve the yield and quality of crops, reduce the use of chemical fertilizers and pesticides, and is suitable for the preparation of biological organic fertilizer and green agricultural production.

The invention provides a bacillus altitudinis Sys-Hup-006 strain for producing huperzine A. The strain is preserved in the China Center for Type Culture Collection, the preservation date is October 31, 2025, and the preservation number is CCTCC NO: M20252401; the 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The bacillus altitudinis Sys-Hup-006 strain provided by the invention can produce huperzine A through metabolism, can be subjected to large-scale fermentation culture in a short time, is relatively low in fermentation cost, is not limited by conditions such as time domain, season and the like, and has a good application prospect. Therefore, the problems that the chemical synthesis cost of huperzine A is relatively high, the steps are complicated and more intermediate pollutants exist can be solved through a microbial fermentation way. Meanwhile, the invention also provides optimized microbial fermentation conditions and a formula of the culture medium, and the yield of huperzine A is relatively high.

The invention discloses a bacterium-enzyme synergistic microbial preparation and a preparation method and application thereof, and the bacterium-enzyme synergistic microbial preparation comprises a mixed bacterium suspension containing bacillus velezensis and bacillus altitudinis; the thallus concentration ratio of the bacillus velezensis to the bacillus altitudinis is (3: 1)-(1: 3); the mixed bacterial suspension also contains cellulase with the mass fraction of 0.5%-3%. After bacteria-enzyme synergistic fermentation treatment, the coordination of conventional chemical components of the tobacco leaves is improved. Moreover, after the bacteria-enzyme synergistic fermentation treatment, the volatile aroma components of the tobacco leaves are remarkably increased, and the content of the volatile aroma components such as megastigmatrienone, benzyl alcohol, phenethyl alcohol and damascenone is remarkably increased.

This invention discloses a microbial agent for regulating the microecology of aquatic bodies and its preparation method. The method includes: inoculating Rhodococcus erythropolis into a liquid culture medium, activating and expanding the culture at 28°C to obtain a Rhodococcus erythropolis fermentation broth with an effective viable count of 2.0×10⁹–2.8×10⁹ CFU / mL; then subjecting the broth to stepwise low-temperature adaptation at 15°C, 10°C, and 5°C to obtain a low-temperature adapted Rhodococcus erythropolis bacterial solution; culturing Bacillus glacialis and Bacillus belesii at 37°C and mixing them at a viable count ratio of 1:1 to obtain a Bacillus bacterial solution; subsequently, compounding the low-temperature adapted Rhodococcus erythropolis bacterial solution and the Bacillus bacterial solution at a viable count ratio of 1:(0.5–4), adding survival protection components and dispersion stabilizing components, and mixing thoroughly to obtain the microbial agent.

The invention discloses a facultative anaerobic bacillus altitudinis strain and an application thereof in tobacco, the facultative anaerobic bacillus altitudinis strain ZY02-1 is classified and named as Bacillus altitudinis, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33916. The invention further discloses a preparation method of the facultative anaerobic bacillus altitudinis strain. The strain is a facultative anaerobic bacterium, can be fermented under two conditions of an aerobic environment and an anaerobic environment, can promote combined nicotine to be converted into free nicotine in a low-oxygen environment, increases the nicotine content of tobacco stems, effectively accelerates decomposition of aroma precursors such as polyphenols and terpenes in the tobacco stems, effectively increases aroma substances of the tobacco stems, and has the advantages of high yield, high yield and the like before re-drying. The bacillus altitudinis ZY02-1 is applied to moisture regaining tobacco stems for stacking fermentation, offensive odor of the fermented tobacco stems is reduced, aroma quality, aroma quantity and concentration are improved, nicotine, megastigmatrienone, geraniol and alpha-pinene are remarkably increased, and aroma components such as alpha-cedrene and ocimene are added.

The invention relates to a bacillus altitudinis ZT61 (bacillus altitudinis ZT61) with an aerobic denitrifying and phosphorus accumulating function and application of the bacillus altitudinis ZT61 in treatment of wastewater with high nitrogen content and phosphorus content. The strain has the capability of synchronously removing ammonia nitrogen and phosphorus, can finally convert nitrogen pollutants into gas products, is suitable for treating high-nitrogen-content organic wastewater, has obvious environmental adaptability and interspecific competitive advantages, can efficiently grow at 15-35 DEG C, and can remove nitrogen and phosphorus. Compared with a traditional denitrification process, the strain is high in denitrification efficiency, low in energy consumption, simple to operate and high in stability, and application of the strain provides a certain basis for sewage treatment.

The invention belongs to the field of microbial agents and plant growth promotion, and particularly relates to bacillus altitudinis YN18 and application thereof in corn growth promotion. The preservation number of the bacillus altitudinis YN18 is CCTCC (China Center for Type Culture Collection) NO: M 20251694, and the bacillus altitudinis YN18 is preserved in the China Center for Type Culture Collection on July 24, 2025; the invention relates to a bacillus altitudinis YN18, which is classified and named as bacillus altitudinis YN18. According to the invention, a strain of bacillus altitudinis YN18 is obtained through separation for the first time, and the strain YN18 has good siderophore production and IAA secretion capabilities, and can significantly promote corn plant height, root length, fresh root weight and dry root weight; therefore, the method has a good application prospect. The bacillus altitudinis YN18 provided by the invention has a remarkable emission reduction effect on NO emission of corn plants, the actually measured emission reduction rate is up to 106.52%, and the bacillus altitudinis YN18 shows excellent greenhouse gas regulation potential.

The application belongs to the technical field of soil conditioners, and particularly relates to a soil conditioner for improving fruit quality in orchards. The soil conditioner is prepared by mixing Bacillus thuringiensis TXB2-5 lyophilized powder, Bacillus pumilus TXB1-8 lyophilized powder and Bacillus altitudinis YLB2-1 lyophilized powder. The preservation number of the Bacillus thuringiensis TXB2-5 is CCTCC No: M 2020343, the preservation number of the Bacillus pumilus TXB1-8 is GDMCC No.62525, and the preservation number of the Bacillus altitudinis YLB2-1 is GDMCC No.62524. The soil conditioner is prepared by mixing Bacillus thuringiensis TXB2-5 lyophilized powder, Bacillus pumilus TXB1-8 lyophilized powder and Bacillus altitudinis YLB2-1 lyophilized powder, and can improve the control effect on diseases in orchards. In addition, the soil conditioner can improve the sugar content and vitamin C content of fruits in orchards, reduce the titratable acid content, and improve the fruit hardness, so that the fruit quality in orchards can be comprehensively improved, and the problem of single function of the conditioner can be effectively solved.

The application relates to the technical field of feed additives, in particular to a feed additive for degrading vomitoxin and application, wherein the feed additive for degrading vomitoxin is composed of, in percentage by weight, Bacillus altitudinis G-1 bacterial liquid: 10-30%; solid carrier: 70-90%. Efficient and stress-resistant Bacillus altitudinis G-1 is obtained through screening, the fermentation liquor is mixed with the treated solid carrier, and low-temperature or spray drying is adopted for shaping. The strain can secrete specific degradation enzyme system to efficiently convert vomitoxin, and can maintain activity in processing and digestive tract environment by virtue of wide temperature range and wide pH tolerance characteristics; in combination with the protection and slow-release effect of the carrier, efficient degradation of vomitoxin and synchronous inhibition of mold growth are realized, and the problems of limited efficiency of existing degradation strains, unstable activity in complex environment and lack of mold regeneration inhibition capacity are solved.

The invention discloses bacillus altitudinis ZXY01 and application of the bacillus altitudinis ZXY01 in improving the content of rapidly available potassium in soil and improving the fertility of the soil. The bacillus altitudinis ZXY01 is classified and named as bacillus altitudinis, and the preservation number of the bacillus altitudinis ZXY01 is CGMCC (China General Microbiological Culture Collection Center) No.36684. The bacillus altitudinis ZXY01 provided by the invention has a good potassium-dissolving function, the research of soil culture experiments proves that a bacterial liquid prepared from the bacillus altitudinis ZXY01 can be directly used as an agricultural microbial potassium-dissolving bacterial agent fertilizer, and due to the direct action of the bacillus altitudinis ZXY01, the potassium-dissolving effect of the bacterial liquid is greatly improved. In the plant planting process, the content of rapidly available potassium in the soil can be effectively increased, the soil fertility is enriched, the forms of a non-exchange state and a mineral state of potassium in the soil are further changed, a new way is provided for a novel agricultural microbial potassium-dissolving bacterial agent fertilizer, and the application prospect is wide.

The present invention addresses the problem of providing a microbial material that can also be applied to agricultural crop cultivation in a dry area.　The present invention provides the following endospore-forming bacteria (A) to (C) having plant-growth promoting activity in a dry environment. (A) Either Bacillus thuringiensis TTREp1 strain identified by the accession number NITE BP-04159 or Bacillus altitudinis TCo-SSREp1 strain identified by the accession number NITE BP-04160, or a mutant strain thereof (B) Paenibacillus polysaccharolyticus TP-WSREp1 strain identified by the accession number NITE BP-04163, or a mutant strain thereof (C) either Priestia aryabhattai TTREn1 strain identified by the accession number NITE BP-04165 or Priestia megaterium TC-WSREn1 strain identified by the accession number NITE BP-04166, or a mutant strain thereof

The invention relates to the technical field of microorganisms, in particular to a bacillus altitudinis BW65 capable of resisting pear white rot and application of the bacillus altitudinis BW65 in disease resistance, yield increase and quality improvement, and the strain is separated from rhizosphere soil of peach trees and is classified and named as Bacillus altitudinis BW65. The strain has the capabilities of producing IAA, fixing nitrogen and decomposing organic phosphorus and inorganic phosphorus, can remarkably promote the growth of plants such as pears and tomatoes, improve the root morphology and improve the biomass and the fruit quality, and has an antagonistic effect on various pathogenic bacteria such as rice blast, alternaria alternata, fusarium graminearum and hard funguses. By applying the microbial agent, the crop yield and quality can be improved, the use of chemical fertilizers and pesticides is reduced, and the microbial agent is suitable for bio-organic fertilizer preparation and green agricultural production.

The application relates to a bacillus subtilis mixing and crushing device for preparing bacillus subtilis suspension, which comprises a mixer, a support frame fixedly connected to the outside of the mixer, a crusher fixedly connected to the bottom of the support frame, and a middle pipe fixedly connected between the mixer and the crusher. The application has the beneficial effect that when the crushed bacillus subtilis does not meet the particle size requirement, the electromagnetic valve b at the fixed cylinder can be opened, and under the action of the driving motor b driving the spiral auger to rotate, the bacillus subtilis can rise to the middle pipe through the bottom of the crusher, be re-conveyed to the middle pipe through the conveying box, and be crushed again in the crusher. The above structure can complete multiple crushing without manual intervention, which not only reduces the labor intensity of workers, but also avoids production interruption caused by manual operation, and ensures the continuity of the crushing operation.

The invention discloses a bacillus altitudinis strain and application thereof. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.36336. The strain has efficient cellulose degradation capacity, fermentation crude enzyme liquid has degradation activity on filter paper, CMC-Na, Avicel PH101 and other substrates, the enzyme activity of the filter paper reaches 1.12 FU / mL, and the enzyme activity of sodium carboxymethyl cellulose reaches 10.68 CU / mL. An enzymatic property research shows that the optimum pH value of the enzyme is 5-7, the optimum reaction temperature is 55 DEG C, and the enzyme has good thermal stability at 40-60 DEG C. When the cellulase is applied to degradation of reed straws, the yield of reducing sugar reaches 2862-4590 mg / g, which is obviously superior to that of commercial cellulase (1341-3012 mg / g). Efficient strain resources and technical support are provided for high-value utilization of the white lake reed waste, the biomass conversion cost is reduced, and the method has important industrial application value and ecological benefits.

The invention belongs to the technical field of soil microorganism regulators, and particularly relates to a facility cucumber soil microorganism regulator. The invention relates to a facility cucumber soil microorganism regulator, which is prepared by mixing a bacillus altitudinis bacterial solution and a bacillus zanthoxyli bacterial solution according to a volume ratio of 2: (9-3). According to the invention, the bacillus altitudinis liquid and the bacillus zanthoxyli liquid are combined for use to form the soil microorganism conditioning agent, and the soil microorganism conditioning agent has a synergistic effect on inhibiting growth of pathogenic bacteria hyphae of the cucumber fusarium wilt, is beneficial to improving the prevention and control effect on the cucumber fusarium wilt, and can provide support for developing a biocontrol agent for preventing and controlling the cucumber fusarium wilt.

This invention discloses a strain of *Bacillus glabra*, a microbial inoculant, and its applications. *Bacillus glabra* (… Bacillus altitudinis Named Bacillus altitudinis The strain number is M1118, the accession number is GDMCC NO:67333, and the accession date is November 21, 2025. Using the *Bacillus hygroscopicus* strain of this invention to extract flavonoids from bamboo leaves significantly improves extraction efficiency: through microbial fermentation, the extraction rate of bamboo leaf flavonoids is increased by more than 50% compared to traditional methods. It is environmentally friendly: the fermentation conditions are mild, requiring no heating or electrical equipment. It is simple to operate and low in cost: the preparation of the inoculum and the fermentation process are easily scalable, reducing the cost of bamboo leaf processing. It allows for comprehensive resource utilization: the fermentation residue can be converted into organic fertilizer, achieving full resource utilization.

This invention provides a *Bacillus hygroscopicus* strain and its application, relating to the field of microbial fermentation technology. The strain is named *Bacillus hygroscopicus* (…). Bacillus altitudinis Bacillus subtilis (W09), deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, China, Institute of Microbiology, Chinese Academy of Sciences, on December 26, 2025, with accession number CGMCC No. 37218. This invention provides Bacillus subtilis (W09). Bacillus altitudinis W09 can be used to increase the content of schisandrol A in the fermentation products of Schisandra chinensis vine stems.

The application discloses a Bacillus altitudinis JYD51 and application thereof in aquaculture, which has been preserved in the China General Microbiological Culture Collection Center on October 10, 2024, and the preservation number is CGMCC No.32164. The JYD51 strain can be fermented by using a culture medium rich in organic matter, and the fermentation product has no toxic effect on Penaeus vannamei and crabs, can inhibit the infection of hepatopancreas ciliates on the Penaeus vannamei and the harm of Paramoeba on the crabs. Meanwhile, the microbial agent prepared by the JYD51 strain and Bacillus sphaericus JYQ7 can effectively control the harm of the hepatopancreas ciliates on the Penaeus vannamei and the Paramoeba on the crabs, purify the water environment for the growth of the Penaeus vannamei and the crabs, and improve the survival rate and product quality of the Penaeus vannamei and the crabs in aquaculture.

The invention discloses a method for producing feruloyl esterase by utilizing bacillus altitudinis and application of the feruloyl esterase. The invention belongs to the technical field of microorganisms, and provides bacillus altitudinis ZH-1 capable of producing feruloyl esterase, the bacillus altitudinis ZH-1 can produce feruloyl esterase at high yield, and the bacillus altitudinis ZH-1 is specifically embodied in that the feruloyl esterase activity in fermentation liquor can reach 254U / L after the bacillus altitudinis ZH-1 is inoculated into a fermentation culture medium for fermentation for 72 hours, so that the feruloyl esterase activity can reach 254U / L, and the feruloyl esterase activity can reach 254U / L; the bacillus altitudinis ZH-1 has a huge application prospect in the production of feruloyl esterase and ferulic acid.

The invention provides a bacillus altitudinis strain DAO2-11 and application thereof in tobacco disease resistance, and belongs to the technical field of biological control. The bacillus altitudinis strain DAO2-11 is named as Bacillus altitudinis in taxonomy, is preserved in Guangdong Microbial Culture Collection Center, and is located on the 5th floor, No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou City. The bacillus altitudinis strain DAO2-11 has the taxonomy name of Bacillus altitudinis and is preserved in the Guangdong Microbial Culture Collection Center; the preservation date is March 14, 2025; the preservation number is GDMCC No: 66017. The bacillus altitudinis DAO2-11 provided by the invention has a remarkable control effect in a pot experiment, can promote the growth of tobacco, and has a great practical value.

A composite bacterial flora for treating benzotriazole wastewater. A bacterial strain composition is provided, the composition comprising the following bacterial strains: (a) Bacillus altitudinis (Bacillus altitudinis) with a preservation number of CGMCC 37186 Bacillus altitudinis ) SD1, (b) Saprospira sp. (Saprospira sp.) with a preservation number of CGMCC 37187 Aquamicrobium defluvii ) SD4, and (c) Pseudomonas aeruginosa (Pseudomonas aeruginosa) with a preservation number of CGMCC 37189 Pseudomonas aeruginosa ) SD32.

The present invention concerns novel spermidine and agmatine producing bacterial strains belonging to the species Bacillus subtilis, Bacillus pumilus and Bacillus altitudinis and preparations thereof as well as food, feed, pharmaceutical, cosmetical, agricultural and laundry compositions containing such strains or preparations thereof and concerns further generally the use of strains of the species Bacillus pumilus and Bacillus altitudinis to produce spermidine or agmatine, in particular in food, feed, pharmaceutical, cosmetical, agricultural, cleaning and laundry compositions.

The application provides a compound microbial agent for promoting growth of tobacco plants and application thereof. The compound microbial agent comprises Bacillus altitudinis ZXY01, Bacillus subtilis LGT-2 and Rhodopseudomonas palustris. The compound microbial agent of the application comprises Bacillus altitudinis ZXY01 which can effectively promote release of potassium ions, help to improve the potassium nutrient level of tobacco plants, and provide support for stem robustness and material transportation; Rhodopseudomonas palustris has nitrogen fixation effect, can increase nitrogen source supply, and provide material basis for leaf development; Bacillus subtilis LGT-2 can promote root development, improve nutrient absorption efficiency, synergistically improve growth vigor of tobacco plants, increase environmental tolerance of the whole microbial group, and regulate the growth rate of the other two strains through metabolic products, thereby avoiding the problem of over-proliferation of a single strain due to the absence of Bacillus subtilis, and enabling the three strains to form a stable microbial group in the rhizosphere.

This invention discloses a strain of *Bacillus hygroscopicus* with functions of phosphorus solubilization, promoting plant growth, and enhancing plant drought resistance, and its applications. The *Bacillus hygroscopicus* is *Bacillus hygroscopicus*. Bacillus height Strain B27, with its registration number CGMCC No. 36877 at the China General Microbiological Culture Collection Center, has been shown to exhibit significant improvements in several experiments. In tobacco inoculation experiments, compared to the control group, the B27-inoculated tobacco seedlings showed a significant increase in lateral root number, root hair length, and aboveground and belowground fresh weight. Similarly, in apple rootstock inoculation experiments, compared to the control group, the B27-inoculated apple plants showed significant increases in plant height, aboveground fresh weight, root surface area, root tip number, root hair length, root hair quantity, and phosphorus content. Finally, in Arabidopsis thaliana inoculation experiments, compared to the control group, the B27-inoculated Arabidopsis seedlings showed a significant improvement in drought resistance.

The invention discloses serine type carboxypeptidase for inhibiting growth of blue-green algae and belongs to the technical field of biology and environmental protection, bacillus altitudinis FY1 is screened and separated from a rice field water sample, it is found that protein serine type carboxypeptidase exists in the FY1 in the peptidoglycan biosynthesis process, the nucleotide sequence of the protein serine type carboxypeptidase is shown as SEQ ID 0.1 in a sequence table, and the nucleotide sequence of the protein serine type carboxypeptidase is shown as SEQ ID NO. The serine type carboxypeptidase is targeted to cell walls of cyanobacteria, has an efficient splitting effect on the cyanobacteria, can kill harmful cyanobacteria and is used for treating cyanobacteria bloom.