53 results about "Bacillus altitudinis" patented technology

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis JSF-9 and a complex microbial inoculant and application thereof.The strain JSF-9 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high. The antibacterial effect on staphylococcus aureus, salmonella typhimurium and shigella flexneri is achieved; the strain has a good bagasse fermentation capability, for example, after the strain, bacillus altitudinis MLF-1 and bacillus safensis MLL-5 are prepared into a mixed microbial inoculum, the bagasse fermentation capability is better, and verification shows that the complex microbial inoculum can effectively inhibit the reproduction of harmful bacteria, so that the preservation period of fermented feed is prolonged, and the yield of the fermented feed is improved. Animal feeding experiments find that the nutritional value and the disease resistance of the complex microbial inoculant fermented bagasse are remarkably improved, 10-15% of the complex microbial inoculant fermented bagasse is added into basic ration, and the cost-reducing and effect-increasing effects are achieved for feeding Jersey cattle.

The invention relates to a microbial strain and application thereof, in particular to bacillus altitudinis, a microbial agent prepared from the bacillus altitudinis and application of the microbial agent. The invention provides bacillus altitudinis CGMCC (China General Microbiological Culture Collection Center) No.33692, which has the functions of improving the fertility of soil, promoting the growth of crops and enhancing the stress resistance of the crops. And the iron carrier can be synthesized and has the capabilities of secreting auxin, fixing nitrogen and dissolving phosphorus. When applied to corn, the corn germination rate can be remarkably increased, the corn plant height, the leaf area, the dry weight and the wet weight are increased, and corn growth is promoted. When applied to tomatoes, the compound can promote tomato growth, effectively improve the activity of a tomato antioxidant system, enhance photosynthesis and significantly improve the low-temperature resistance of tomatoes. When being used as a soil conditioner, the soil conditioner can effectively improve the soil fertility, reduce the pH value, improve the conductivity and effectively improve the physicochemical properties, and provides a good soil environment condition for the growth and development of corn and tomatoes.

The invention discloses bacillus altitudinis and an application of the bacillus altitudinis in prevention and treatment of plant diseases. The bacillus altitudinis CDJ1 is preserved in Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65289. The bacillus altitudinis CDJ1 disclosed by the invention can be used for effectively resisting pectobacterium taro, and in the formula, the bacillus altitudinis CDJ1 can be used for effectively resisting Desia Dabesii, Xanthomonas citrus pathogenic variants, Xanthomonas citrus mango pathogenic variants, Xanthomonas campestris, Xanthomonas sp., Xanthomonas sp., Xanthomonas sp. And Xanthomonas arborescens plum pathogenic variants, so that the bacillus altitudinis CDJ1 can be used for effectively resisting pectobacterium taro and the bacillus altitudinis CDJ1 can be used for effectively resisting pectobacterium taro. The composition has a good prevention and treatment effect on various plant diseases. When the bacillus altitudinis CDJ1 is prepared into a microbial agent, the use of chemical pesticides can be reduced, and the bacillus altitudinis CDJ1 has a wide application prospect in the field of agricultural biocontrol.

The invention relates to a composite flora for treating benzotriazole wastewater. According to the present invention, a strain composition is provided, and the strain composition comprises the following strains: (a) Bacillus altitudinis SD1 having a preservation number of CGMCC 37186, (b) Aquammicrobe defluvii SD4 having a preservation number of CGMCC 37187, and (c) Pseudomonas aeruginosa SD32 having a preservation number of CGMCC 37189, and the strain composition comprises the following strains: (a) Bacillus altitudinis SD1 having a preservation number of CGMCC 37186, (b) Aquammicrobe defluvii SD4 having a preservation number of CGMCC 37187, and (c) Pseudomonas aeruginosa SD32 having a preservation number of CGMCC 37189.

The invention provides application of bacillus altitudinis H18 and a glucoside hydrolase gene thereof in improving rumen degradation of rice straws, and belongs to the technical field of straw reutilization. The bacillus altitudinis H18 is separated from goat rumen fluid, the bacillus altitudinis H18 can promote degradation of rice straw cellulose, and reference is provided for feed application of rice straw. Sequencing analysis finds that a whole genome of the strain H18 contains a large number of genes related to glucoside hydrolase GH, carbohydrate hydrolase CE and the like, and the content of neutral detergent fibers and lignin in the rice straw can be remarkably reduced by performing fermentation treatment on the rice straw by using the strain H18 through an in-vitro fermentation method. The strain can be used as a bacterial strain for feed application of the rice straws so as to improve the conversion rate and the utilization rate of the rice straws.

The invention provides bacillus altitudinis CL6 with a morchella esculenta continuous cropping resisting effect and application of the bacillus altitudinis CL6, and belongs to the technical field of microorganisms. The bacillus altitudinis CL6 with a continuous cropping resisting effect on morchella is separated from morchella rhizosphere soil and is preserved in the China Center for Type Culture Collection on March 18, 2025, and the preservation number is CCTCC NO: M 2025516. The bacillus altitudinis CL6 disclosed by the invention can be used as an auxiliary added beneficial microbial agent in the cultivation production of the morchella to resist the continuous cropping harm of the morchella, and finally, the cultivation stability of the morchella is improved. The strain provided by the invention can promote the continuous cropping resistance effect of morchella esculenta and increase the yield of morchella esculenta. And a green and efficient anti-continuous cropping biological solution is provided for the morchella esculenta cultivation industry.

The application discloses a high Bacillus altitudinis SW2, belongs to the field of degrading bacteria screening, and the high Bacillus altitudinis SW2 has been preserved in the China General Microbiological Culture Collection Center on May 8, 2024, has a preservation number of CGMCC No.30525, and has a preservation address of No.3, Xili, Beichen West Road, Chaoyang District, Beijing City; is classified and named as the high Bacillus altitudinis. The strain is obtained through screening, purification and identification technologies from grape branch compost, and through research on strain characteristics, fermentation conditions and cellulase activity of the strain, it is shown that the enzyme activity of the strain is 547.82 U / g under the condition of 30 DEG C and PH=7, and when 5% casein is added as a protective agent, the effective viable cell number of the high Bacillus altitudinis SW2 is the highest and can reach 1.73*10 14 cfu / g.

The invention provides bacillus altitudinis JZBQ5 and application of the bacillus altitudinis JZBQ5 in plant disease prevention and growth promotion. The preservation number of the bacillus altitudinis JZBQ5 is CGMCC (China General Microbiological Culture Collection Center) The bacillus altitudinis JZBQ5 can improve the disease resistance of strawberries, and has a good control effect on powdery mildew and anthracnose of strawberries. Meanwhile, growth of cucumbers and wheat is promoted, and plant diseases such as cucumber root rot and wheat stem rot are effectively prevented and controlled. The strain provides a new strain resource for biological control of crop diseases such as strawberry powdery mildew, and a theoretical basis is provided for a safe and efficient green biocontrol preparation.

The invention relates to a mild thermal enhanced photodynamic spore inactivation method, which combines a photodynamic technology with 65 DEG C mild thermal treatment, and is used for killing bacillus altitudinis spores and bacillus safensis spores in a germination stage. The method disclosed by the invention has an excellent killing effect on spores of bacillus altitudinis and spores of bacillus safensis, can realize efficient inactivation of the spores in meat products, solves the defects of the existing photodynamic technology for inactivating the spores, adopts a sterilization temperature far lower than a commercial sterilization temperature, can better maintain the flavor and quality of foods, and is suitable for industrial production. And the method has universality in food industry application.

The invention relates to the technical field of biopesticides, in particular to a mixed biocontrol microbial inoculum for preventing and treating blueberry gray mold and application of the mixed biocontrol microbial inoculum. The invention relates to a mixed biocontrol microbial agent for preventing and treating blueberry gray mold. The mixed biocontrol microbial agent is prepared from the following components: pseudomonas chlororaphis A4, bacillus altitudinis LY-3, bacillus altitudinis Y-14 and bacillus altitudinis An-2. The four bacteria are mixed and prepared, the four bacteria have an inhibiting effect on the blueberry botrytis cinerea when being independently used, and the four bacteria are mixed and prepared to cooperate with one another to inhibit growth of botrytis cinerea hyphae; the four components are used together, so that gray mold of blueberry fruits in the field can be effectively prevented and treated, and the yield of blueberries is increased.

The invention belongs to the technical field of microorganisms, and discloses a novel bacillus altitudinis strain ZL-ZLY-01 and application of the novel bacillus altitudinis strain ZL-ZLY-01 in preparation of collagenase through fermentation. The preservation number of the bacillus altitudinis ZL-ZLY-01 is CGMCC (China General Microbiological Culture Collection Center) NO. 27359. The collagenase produced by fermentation of the bacillus altitudinis ZL-ZLY-01 can be applied to preparation of products containing the collagenase.

The present invention relates to the field of microbial technology, and in particular to Bacillus grosvenoris MLF-1, a composite bacterial agent and an application thereof. The Bacillus grosvenoris strain MLF-1 of the present invention is isolated by a research group from the rectal contents of healthy cattle. Testing shows that the strain has good β-1,4-glucanase production ability and has antibacterial effects on Enterobacter aerogenes, Staphylococcus aureus, Salmonella typhimurium and Escherichia coli. The Bacillus grosvenoris MLF-1 has good fermentation ability for monk fruit residue. After the Bacillus grosvenoris MLF-1 is mixed with Bacillus licheniformis strain JSF-9 and Bacillus saffron strain MLL-5 to prepare a composite bacterial agent, it is found that the nutritional value and disease resistance of the monk fruit residue fermented with the composite bacterial agent are significantly improved. Adding 10%-15% of the composite bacterial agent to the basal diet can reduce the cost and increase the efficiency of feeding Jersey cattle.

The invention belongs to the technical field of microorganisms, and particularly relates to a bacillus altitudinis strain and application thereof, the bacillus altitudinis strain is a bacillus altitudinis strain H50 and has been preserved in China General Microbiological Culture Collection Center (CGMCC) on December 16, 2024, and the preservation number is CGMCC No.33070. The bacillus altitudinis strain is a bacillus altitudinis strain H50 which has been preserved in China General Microbiological Culture Collection Center (CGMCC). The bacillus altitudinis strain H50 disclosed by the invention is a strain with broad-spectrum antibacterial activity, and the strain particularly has an obvious antagonistic effect on tritirachium album, so that effective treatment on tritirachium album and escherichia coli infection is realized.

The application belongs to the technical field of microorganisms, and particularly relates to a bacillus altitudinis BW65 strain against pear white line feather disease and application of the bacillus altitudinis BW65 strain in disease resistance, yield increase and quality improvement. Bacillus altitudinis The strain is isolated from the rhizosphere soil of a peach tree and is classified and named as the BW65 strain. The strain has the abilities of IAA production, nitrogen fixation, organic phosphorus and inorganic phosphorus decomposition, can significantly promote the growth of pear, tomato and other plants, improve the root morphology, increase the biomass and fruit quality, and has antagonistic effects on multiple pathogenic bacteria such as rice blast, alternaria alternata, fusarium graminearum and botryosphaeria dothidea. Application of the bacterial agent can improve the yield and quality of crops, reduce the use of chemical fertilizers and pesticides, and is suitable for the preparation of biological organic fertilizer and green agricultural production.

The invention relates to a complex microbial inoculant BAA and application thereof, the active ingredient of the complex microbial inoculant BAA comprises at least one strain of bacillus amyloliquefaciens CJ-796 and bacillus altitudinis CJ-365, the viable bacterium concentration of the complex microbial inoculant BAA is 1 * 10 < 8 > CFU / mL, the active ingredient of the complex microbial inoculant BAA is at least one strain of bacillus amyloliquefaciens CJ-796 and bacillus altitudinis CJ-365, and the viable bacterium concentration of the complex microbial inoculant BAA is 1 * 10 < 8 > CFU / mL. By performing root irrigation treatment on plants, the tobacco black shank can be effectively prevented and treated, tobacco plant growth can be remarkably promoted, the tobacco black shank prevention effect is 75% or above, the plant growth promoting effect can reach 50% or above, and the composition has the advantages of being safe, free of residues and environmentally friendly, meets the requirement for pesticide safety and has a wide application prospect. The potential application value is realized in the aspect of biological prevention and control of the tobacco black shank.

The invention provides a bacillus altitudinis Sys-Hup-006 strain for producing huperzine A. The strain is preserved in the China Center for Type Culture Collection, the preservation date is October 31, 2025, and the preservation number is CCTCC NO: M20252401; the 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The bacillus altitudinis Sys-Hup-006 strain provided by the invention can produce huperzine A through metabolism, can be subjected to large-scale fermentation culture in a short time, is relatively low in fermentation cost, is not limited by conditions such as time domain, season and the like, and has a good application prospect. Therefore, the problems that the chemical synthesis cost of huperzine A is relatively high, the steps are complicated and more intermediate pollutants exist can be solved through a microbial fermentation way. Meanwhile, the invention also provides optimized microbial fermentation conditions and a formula of the culture medium, and the yield of huperzine A is relatively high.

The invention discloses a bacterium-enzyme synergistic microbial preparation and a preparation method and application thereof, and the bacterium-enzyme synergistic microbial preparation comprises a mixed bacterium suspension containing bacillus velezensis and bacillus altitudinis; the thallus concentration ratio of the bacillus velezensis to the bacillus altitudinis is (3: 1)-(1: 3); the mixed bacterial suspension also contains cellulase with the mass fraction of 0.5%-3%. After bacteria-enzyme synergistic fermentation treatment, the coordination of conventional chemical components of the tobacco leaves is improved. Moreover, after the bacteria-enzyme synergistic fermentation treatment, the volatile aroma components of the tobacco leaves are remarkably increased, and the content of the volatile aroma components such as megastigmatrienone, benzyl alcohol, phenethyl alcohol and damascenone is remarkably increased.

This invention discloses a microbial agent for regulating the microecology of aquatic bodies and its preparation method. The method includes: inoculating Rhodococcus erythropolis into a liquid culture medium, activating and expanding the culture at 28°C to obtain a Rhodococcus erythropolis fermentation broth with an effective viable count of 2.0×10⁹–2.8×10⁹ CFU / mL; then subjecting the broth to stepwise low-temperature adaptation at 15°C, 10°C, and 5°C to obtain a low-temperature adapted Rhodococcus erythropolis bacterial solution; culturing Bacillus glacialis and Bacillus belesii at 37°C and mixing them at a viable count ratio of 1:1 to obtain a Bacillus bacterial solution; subsequently, compounding the low-temperature adapted Rhodococcus erythropolis bacterial solution and the Bacillus bacterial solution at a viable count ratio of 1:(0.5–4), adding survival protection components and dispersion stabilizing components, and mixing thoroughly to obtain the microbial agent.

The invention relates to the technical field of microorganisms, in particular to bacillus altitudinis MLF-1 and a complex microbial inoculant and application thereof.The bacillus altitudinis strain MLF-1 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high; the antibacterial effect is achieved on enterobacter aerogenes, staphylococcus aureus, salmonella typhimurium and escherichia coli; the bacillus altitudinis MLF-1 has good fermentation capacity on the siraitia grosvenorii residues, after the bacillus altitudinis MLF-1 is mixed with the bacillus licheniformis strain JSF-9 and the bacillus safensis strain MLL-5 to prepare the complex microbial inoculant, the nutritional value and the disease resistance of the siraitia grosvenorii residues fermented by the complex microbial inoculant are remarkably improved, 10%-15% of the complex microbial inoculant is added into a basic ration, and the content of the complex microbial inoculant in the siraitia grosvenorii residues fermented by the complex microbial inoculant is reduced. The Jersey cattle feed has the effects of reducing cost and improving efficiency for feeding Jersey cattle.

The invention discloses a facultative anaerobic bacillus altitudinis strain and an application thereof in tobacco, the facultative anaerobic bacillus altitudinis strain ZY02-1 is classified and named as Bacillus altitudinis, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33916. The invention further discloses a preparation method of the facultative anaerobic bacillus altitudinis strain. The strain is a facultative anaerobic bacterium, can be fermented under two conditions of an aerobic environment and an anaerobic environment, can promote combined nicotine to be converted into free nicotine in a low-oxygen environment, increases the nicotine content of tobacco stems, effectively accelerates decomposition of aroma precursors such as polyphenols and terpenes in the tobacco stems, effectively increases aroma substances of the tobacco stems, and has the advantages of high yield, high yield and the like before re-drying. The bacillus altitudinis ZY02-1 is applied to moisture regaining tobacco stems for stacking fermentation, offensive odor of the fermented tobacco stems is reduced, aroma quality, aroma quantity and concentration are improved, nicotine, megastigmatrienone, geraniol and alpha-pinene are remarkably increased, and aroma components such as alpha-cedrene and ocimene are added.

The invention relates to a bacillus altitudinis ZT61 (bacillus altitudinis ZT61) with an aerobic denitrifying and phosphorus accumulating function and application of the bacillus altitudinis ZT61 in treatment of wastewater with high nitrogen content and phosphorus content. The strain has the capability of synchronously removing ammonia nitrogen and phosphorus, can finally convert nitrogen pollutants into gas products, is suitable for treating high-nitrogen-content organic wastewater, has obvious environmental adaptability and interspecific competitive advantages, can efficiently grow at 15-35 DEG C, and can remove nitrogen and phosphorus. Compared with a traditional denitrification process, the strain is high in denitrification efficiency, low in energy consumption, simple to operate and high in stability, and application of the strain provides a certain basis for sewage treatment.

The invention belongs to the field of microbial agents and plant growth promotion, and particularly relates to bacillus altitudinis YN18 and application thereof in corn growth promotion. The preservation number of the bacillus altitudinis YN18 is CCTCC (China Center for Type Culture Collection) NO: M 20251694, and the bacillus altitudinis YN18 is preserved in the China Center for Type Culture Collection on July 24, 2025; the invention relates to a bacillus altitudinis YN18, which is classified and named as bacillus altitudinis YN18. According to the invention, a strain of bacillus altitudinis YN18 is obtained through separation for the first time, and the strain YN18 has good siderophore production and IAA secretion capabilities, and can significantly promote corn plant height, root length, fresh root weight and dry root weight; therefore, the method has a good application prospect. The bacillus altitudinis YN18 provided by the invention has a remarkable emission reduction effect on NO emission of corn plants, the actually measured emission reduction rate is up to 106.52%, and the bacillus altitudinis YN18 shows excellent greenhouse gas regulation potential.

The invention relates to the technical field of agricultural biology, in particular to bacillus altitudinis and application thereof, and is particularly suitable for improving the growth performance of tropical corn in a low absorbable iron stress environment. The bacillus altitudinis has been preserved in Institute of Microbiology, Academy of Sciences, Guangdong Province on June 3, 2025, and the address of the bacillus altitudinis is the fifth floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City; the preservation number of the strain is GDMCC NO: 66398. The secretion of the bacillus altitudinis can promote the absorption of insoluble iron ions and other important nutrient elements by the tropical corn, so that the biological generation of chlorophyll is promoted, and the growth of the tropical corn is promoted.

The present invention provides a strain of Bacillus altitudinis NM62 and its application in promoting growth and disease resistance, with the preservation number of CGMCC NO.31272. This strain of Bacillus altitudinis has a good disease prevention effect on tobacco black shank disease and can promote the growth of tobacco plants. This strain provides a new strain resource for the biological control of tobacco black shank disease and provides a theoretical basis for the development of compound biocontrol agents that are environmentally friendly, biosafe and have a long-lasting control effect.

The application belongs to the technical field of soil conditioners, and particularly relates to a soil conditioner for improving fruit quality in orchards. The soil conditioner is prepared by mixing Bacillus thuringiensis TXB2-5 lyophilized powder, Bacillus pumilus TXB1-8 lyophilized powder and Bacillus altitudinis YLB2-1 lyophilized powder. The preservation number of the Bacillus thuringiensis TXB2-5 is CCTCC No: M 2020343, the preservation number of the Bacillus pumilus TXB1-8 is GDMCC No.62525, and the preservation number of the Bacillus altitudinis YLB2-1 is GDMCC No.62524. The soil conditioner is prepared by mixing Bacillus thuringiensis TXB2-5 lyophilized powder, Bacillus pumilus TXB1-8 lyophilized powder and Bacillus altitudinis YLB2-1 lyophilized powder, and can improve the control effect on diseases in orchards. In addition, the soil conditioner can improve the sugar content and vitamin C content of fruits in orchards, reduce the titratable acid content, and improve the fruit hardness, so that the fruit quality in orchards can be comprehensively improved, and the problem of single function of the conditioner can be effectively solved.

The application relates to the technical field of feed additives, in particular to a feed additive for degrading vomitoxin and application, wherein the feed additive for degrading vomitoxin is composed of, in percentage by weight, Bacillus altitudinis G-1 bacterial liquid: 10-30%; solid carrier: 70-90%. Efficient and stress-resistant Bacillus altitudinis G-1 is obtained through screening, the fermentation liquor is mixed with the treated solid carrier, and low-temperature or spray drying is adopted for shaping. The strain can secrete specific degradation enzyme system to efficiently convert vomitoxin, and can maintain activity in processing and digestive tract environment by virtue of wide temperature range and wide pH tolerance characteristics; in combination with the protection and slow-release effect of the carrier, efficient degradation of vomitoxin and synchronous inhibition of mold growth are realized, and the problems of limited efficiency of existing degradation strains, unstable activity in complex environment and lack of mold regeneration inhibition capacity are solved.

The invention relates to the technical field of microorganisms, in particular to bacillus altitudinis as well as a complex microbial inoculant and application thereof. The invention provides bacillus altitudinis, which is derived from intestinal tracts of earthworms, is named as DBN-EW28 and is preserved in China General Microbiological Culture Collection Center on February 14, 2025, the preservation number is CGMCC No.33530, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing. The bacillus altitudinis DBN-EW28 strain disclosed by the invention has relatively high cellulase activity and antibacterial property, and is used for degrading crop straws when being combined with other strains, so that the contents of cellulose and hemicellulose in the crop straws are remarkably reduced, and the degradation effect of straw lignocellulose and the conversion rate of reducing sugar are greatly improved.

The invention discloses bacillus altitudinis ZXY01 and application of the bacillus altitudinis ZXY01 in improving the content of rapidly available potassium in soil and improving the fertility of the soil. The bacillus altitudinis ZXY01 is classified and named as bacillus altitudinis, and the preservation number of the bacillus altitudinis ZXY01 is CGMCC (China General Microbiological Culture Collection Center) No.36684. The bacillus altitudinis ZXY01 provided by the invention has a good potassium-dissolving function, the research of soil culture experiments proves that a bacterial liquid prepared from the bacillus altitudinis ZXY01 can be directly used as an agricultural microbial potassium-dissolving bacterial agent fertilizer, and due to the direct action of the bacillus altitudinis ZXY01, the potassium-dissolving effect of the bacterial liquid is greatly improved. In the plant planting process, the content of rapidly available potassium in the soil can be effectively increased, the soil fertility is enriched, the forms of a non-exchange state and a mineral state of potassium in the soil are further changed, a new way is provided for a novel agricultural microbial potassium-dissolving bacterial agent fertilizer, and the application prospect is wide.

The present invention addresses the problem of providing a microbial material that can also be applied to agricultural crop cultivation in a dry area.　The present invention provides the following endospore-forming bacteria (A) to (C) having plant-growth promoting activity in a dry environment. (A) Either Bacillus thuringiensis TTREp1 strain identified by the accession number NITE BP-04159 or Bacillus altitudinis TCo-SSREp1 strain identified by the accession number NITE BP-04160, or a mutant strain thereof (B) Paenibacillus polysaccharolyticus TP-WSREp1 strain identified by the accession number NITE BP-04163, or a mutant strain thereof (C) either Priestia aryabhattai TTREn1 strain identified by the accession number NITE BP-04165 or Priestia megaterium TC-WSREn1 strain identified by the accession number NITE BP-04166, or a mutant strain thereof

The present invention relates to a fermentation process utilizing seaweed biomass co-inoculated with a yeast strain and a carbohydrate source, wherein the fermentation process is performed in the presence of one or more Bacillus strains to increase the production of vitamin K2. In one embodiment, the seaweed is Kappaphycus alvarezii, the yeast is Saccharomyces cerevisiae, Candida utilis, or the mixture thereof, and the one or more Bacillus strains is Bacillus altitudinis, Bacillus pumilus, and / or Bacillus subtilis.