The invention discloses an application and method of Tween 80 combined immobilization technology enhanced microorganisms in degradation of
naphthalene pollution in water, the main body of the combined immobilization technology is SA / CS at Tween 80
hydrogel microspheres loaded with NM-1
flora, and the NM-1
flora is
halotolerant bacteria obtained by enrichment and
domestication from deep-sea sediments of a certain sea
ditch. The SA / CS at Tween 80 microspheres are formed by crosslinking
sodium alginate (SA),
chitosan (CS) and Tween 80, hydroxyl functional groups of the Tween 80 and SA / CS are crosslinked to enhance the
mechanical strength of the microspheres and regulate and control the pore structure to improve the
mass transfer efficiency, hydrophobic-CH functional groups at the end positions of the Tween 80 strengthen the adsorption capacity to
naphthalene, meanwhile, a co-crosslinking structure at the interface of the SA / CS at Tween 80
hydrogel microspheres forms a biochemical metabolic reaction buffer area, and the adsorption capacity to
naphthalene is improved. The direct
toxicity stress of the high-concentration naphthalene on the
flora is effectively avoided, and the effective biological removal of the high-concentration naphthalene is realized. The SA / CS-coated Tween 80
hydrogel microspheres prepared by the invention can effectively maintain the efficient
metabolic activity and degradation efficiency of the loaded NM-1 flora, and show excellent cycle stability and good
engineering application potential.