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Method for degrading phenylamine in waste water

A waste water and aniline technology, applied in chemical instruments and methods, water pollutants, biological water/sewage treatment, etc., can solve the problems of aniline degradation and inability to achieve the effect of low cost and guaranteed degradation rate

Inactive Publication Date: 2012-10-17
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing biological methods are only suitable for degrading aniline under low-salt conditions, but cannot degrade aniline under high-salt conditions.

Method used

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  • Method for degrading phenylamine in waste water
  • Method for degrading phenylamine in waste water
  • Method for degrading phenylamine in waste water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 The acquisition of aniline salt-tolerant bacteria

[0018] Methods as below:

[0019] (1) The activated sludge polluted by aniline was enriched and cultured at a temperature of 30°C and 150r / min for 48 hours to obtain an enriched liquid, and the supernatant was diluted in gradient and spread on the beef extract peptone solid medium;

[0020] (2) Acclimatization of aniline resistance ability: inoculate the enriched bacterial liquid on the inorganic salt medium containing saturated aniline solution (ammonium sulfate 0.5g, potassium dihydrogen phosphate 0.5g, sodium chloride 0.5g, ammonium chloride 1g, magnesium sulfate 0.632g, made into 1000ml with distilled water) to domesticate the aniline-resistant ability of the strain, after 48 hours of cultivation, transfer the bacterial liquid to a fresh liquid inorganic salt medium, and gradually increase the aniline concentration during the domestication process, gradually increasing from 50mg / L to 500mg / L;

[0021]...

Embodiment 2

[0026] Example 2 The influence of pH value on the degradation performance of aniline

[0027] Method: The suspensions of four aniline salt-tolerant strains in the logarithmic growth phase were inoculated at 10% (v / v) to pH values ​​of 4.0, 5.0, 6.0, 7.0, 8.0, and 9.0, respectively. In the aniline inorganic salt medium of L (ammonium sulfate 0.5g, potassium dihydrogen phosphate 0.5g, sodium chloride 0.5g, ammonium chloride 1g, magnesium sulfate 0.632g, made into 1000ml with distilled water), the salinity is 10%, Shake culture at 30°C and 150 r / min for 48 hours, measure the degradation rate of aniline after 48 hours, and use photoelectric turbidimetry to measure the growth of the strain. The degradation results are as follows: figure 1 .

[0028] like figure 1 As shown, the four strains grow best when the pH value is 7.0, and when the pH value is 7.0, the degradation rate of aniline reaches the highest, and the preferred pH value of the method of the present invention is 7.0...

Embodiment 3

[0029] Example 3 Effect of initial concentration of aniline on the degradation performance of aniline

[0030] Method: The suspensions of four aniline-salt-tolerant strains in the logarithmic growth phase were inoculated at 10% (v / v) in the aniline inorganic Salt medium (ammonium sulfate 0.5g, potassium dihydrogen phosphate 0.5g, sodium chloride 0.5g, ammonium chloride 1g, magnesium sulfate 0.632g, made into 1000ml with distilled water), the salinity is 10%, at 30℃, Under the conditions of pH=7 and 150r / min, shake culture for 48h. Measure the aniline degradation rate of 48h, utilize photoelectric turbidimetry to measure the growth situation of bacterial strain simultaneously, degradation result is as follows figure 2 .

[0031] like figure 2 As shown, the degradation rate of aniline by the strains decreased with the increase of the initial concentration of aniline, and the growth values ​​of the four strains all began to decline when the aniline concentration exceeded 5...

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Abstract

The invention relates to a method for degrading phenylamine in waste water. The method adopts the technical scheme as follows: inoculating phenylamine halotolerant bacteria in logarithm growing period into the waste water containing the phenylamine; adjusting pH value the mixing to be 4-9 to be cultivated for 48 hours under an oscillation condition. With the adoption of the method, the phenylamine can be effectively degraded under a condition of high-salt, the defects that the high-salt can restrain growth and metabolism of bacterial strains, and the degrading capacity is short can be made up, the degrading capacity of the bacterial strains on the phenylamine under the condition of the high-salt is improved, and the technology support can be provided for the phenylamine treatment under the condition of high-salt.

Description

technical field [0001] The invention relates to a water treatment method, in particular to a treatment method for aniline in waste water under high-salt conditions. Background technique [0002] Aniline, also known as anilin oil, has a strong pungent smell. It is a transparent oily liquid that is colorless or light yellow. Aniline is slightly soluble in water, soluble in solvent gasoline, and easily soluble in ethanol and acetone. , ether, benzene and carbon tetrachloride, etc. Aniline turns brown when exposed to air or sunlight. Aniline is a chemically active substance that can undergo halogenation, diazotization, acetylation, and redox reactions. After reacting with sulfuric acid or hydrochloric acid, it can generate sulfate or hydrochloride. Aniline is a very typical substance among aromatic amines. As an important chemical raw material, it is mainly used in industries such as national defense, dyestuff, rubber, pesticide and medicine. With the rapid development of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/38
Inventor 徐成斌马溪平包坤孟雪莲李瑶瑶牟安逸
Owner LIAONING UNIVERSITY
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