25results about How to "Strong growth" patented technology

The invention discloses a trichoderma virens strain KN-T01, a trichoderma preparation and application of the trichoderma preparation, and belongs to the technical field of biocontrol microbial agents. The invention provides a trichoderma virens strain KN-T01, a trichoderma preparation and application of the trichoderma virens strain KN-T01 and aims to solve the technical problem that a biocontrol microbial agent in the prior art is poor in microbial function effect in a low-temperature environment, the preservation number of the trichoderma virens strain KN-T01 is CCTCC M 20242443, and the trichoderma preparation contains the trichoderma virens strain KN-T01. The trichoderma virens strain KN-T01 and the trichoderma preparation provided by the invention have relatively good low temperature resistance, have relatively good antagonism and hyperparasitism effects on pathogenic bacteria of pepper gray mold and watermelon fusarium wilt, have a good decomposition effect on straws under a low-temperature condition, and have a relatively good promotion effect on growth of tomatoes and corns under a low-temperature condition, so that the trichoderma virens strain KN-T01 and the trichoderma preparation have relatively good application prospects. The trichoderma virens strain KN-T01 and the trichoderma preparation are non-toxic and harmless, and can be applied to agricultural green prevention and control.

The invention discloses three novel corynebacterium glutamicum heat-resistant elements and application thereof, and belongs to the technical field of bioengineering. Three heat-resistant genes are obtained from corynebacterium glutamicum research. The heat-resistant gene and a promoter Ptuf are connected and are combined and transformed in corynebacterium glutamicum. The heat-resistant element is introduced into a plasmid and is expressed in corynebacterium glutamicum. After 24 hours of high-temperature culture at 42 DEG C, the OD600 of the corynebacterium glutamicum containing the heat-resistant element and the plasmid is improved, and the growth condition of the corynebacterium glutamicum at 42 DEG C is obviously superior to that of the starting corynebacterium glutamicum. Therefore, the heat-resistant elements can obviously improve the heat resistance of corynebacterium glutamicum under a high-temperature culture condition. The method has important application value in improvement of the thermal stability of corynebacterium glutamicum and industrial production prospect.

The invention discloses a Chinese wildrye hydroponic nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of plant cultivation. Each liter of the nutrient solution comprises the following components: 80 to 120 mg of ammonium nitrate, 45 to 55 mg of sodium dihydrogen phosphate dihydrate, 80 to 90 mg of potassium sulfate, 110 to 115 mg of anhydrous calcium chloride, 400 to 410 mg of magnesium sulfate heptahydrate, 1.5 to 2.0 mg of manganese chloride tetrahydrate, 0.08 to 0.10 mg of ammonium molybdate tetrahydrate, 1.1 to 1.2 mg of boric acid, 0.02 to 0.05 mg of zinc sulfate heptahydrate, 0.02 to 0.05 mg of copper sulfate pentahydrate, 15 to 35 mg of ferrous sulfate heptahydrate and 20 to 50 mg of disodium ethylene diamine tetraacetate. The hydroponic nutrient solution provided by the invention meets the nutritional requirements of leymus chinensis, seedlings grow quickly and vigorously by utilizing the nutrient solution, the biomass and nutrient content of the overground part are remarkably increased, and the high-quality pasture characteristics of the leymus chinensis are ensured.

The invention relates to the field of plant tissue culture, and discloses a method for culturing tissue culture seedlings of small angel viridae, which comprises the following steps: S1, explant selection and primary culture; s2, carrying out subculture multiplication culture; s3, rooting culture; s4, domesticating and transplanting; in the primary culture stage, MS is used as a basic culture medium, a proper amount of 6-BA, NAA, sucrose and agar are added, and induction of cluster buds is promoted; in the subculture multiplication culture stage, an optimal multiplication culture medium is screened through an orthogonal test, so that the multiplication coefficient is improved; in the rooting stage, key factors such as inorganic salt concentration, NAA and banana puree in MS are optimized, and the number and quality of root systems are improved; in the domestication transplanting stage, the optimal proportion of river sand, coco coir and peat soil is researched, and the transplanting survival rate and the tissue culture seedling quality are effectively improved. By means of the method, efficient propagation of the small angel vitis vinifera and large-scale production of high-quality seedlings are achieved, and important technical support is provided for industrial popularization of the plant.

The invention discloses a method for shortening a grape breeding fruiting period, which comprises the following steps: S1, sowing: accelerating germination and sowing hybrid seeds subjected to low-temperature stratification treatment in the first ten days of February to obtain seedlings; s2, transplanting: in the middle ten days of March, when the seedlings obtained in the S1 grow 5-6 true leaves, transplanting the seedlings into a ridged greenhouse nursery garden; s3, primary grafting: in the last ten days of April, selecting semi-lignified branches with the thickness of more than 0.4 cm and full bud eyes of hybrid seedlings, and grafting the semi-lignified branches to current-year semi-lignified new tips of the cutting seedlings of the rootstock of the Zhichang anti-rootstock No.1; s4, secondary grafting: in the last ten days of May, when the young sprouts of the hybrid seedlings after primary grafting grow to 30-50cm and the thickness is 0.4 cm or more, buds on the semi-lignified young sprouts are adopted for secondary grafting. The method is carried out in a greenhouse, current-year grafting and current-year forming can be achieved by adopting a high-position grafting flat shed erecting potential, the child process is manually intervened, the trunk growth time is skipped, and flowering and fruiting as soon as possible are promoted by utilizing the powerful root system of the Zhichang anti-anvil No.1.

The invention provides a breeding method of a new variety of hemerocallis fulva, and relates to the technical field of plant breeding, and the breeding method comprises the following steps: collecting pollen of three hemerocallis fulva stock seeds of hemerocallis fulva, hemerocallis fulva and hemerocallis fulva, and freezing and storing the pollen; after the hemerocallis fulva germplasm resource garden enters a flowering period, collecting pollen of a variety resource with high ornamental value and good resistance, and freezing and storing; the method provided by the invention can overcome the problem of flowering asynchronism of three stock seeds of the early flower type day lily and other horticultural day lily varieties, fully utilizes the early flowering genes of the stock seeds of the early flower type day lily, transforms the stock seeds into the filial generation through reciprocal cross, and hybridizes the three stock seeds and a plurality of horticultural varieties to obtain the hybrid variety of the early flower type day lily. A large number of novel varieties of hemerocallis fulva with various flower colors, high ornamental value and early flowering period can be obtained, and the urgent demand of the market on the novel varieties of the hemerocallis fulva can be met.

The invention discloses a rhizoma curcumae longae detoxification method by combining indole-3-methanol with stem tip culture, and belongs to the technical field of plant tissue culture detoxification seedling culture. The turmeric detoxification method comprises the following steps: (1) taking turmeric rhizomes as explants, and carrying out subculture to obtain turmeric tissue culture seedlings; (2) culturing the turmeric tissue culture seedling on an indole-3-methanol exogenous treatment culture medium to obtain a mature plant; and (3) cutting a stem tip from the mature plant, and culturing on a stem tip induced differentiation culture medium to obtain the turmeric virus-free tissue culture seedling. The turmeric detoxification tissue culture seedlings are cultured by applying the turmeric detoxification method combining indole-3-methanol exogenous treatment with one-time stem tip culture, the technical difficulty is low, the cost is low, the culture period is short, the regenerated plants are high in detoxification rate and strong in growth vigor, and a technical support is provided for large-scale production of the turmeric detoxification tissue culture seedlings.

The invention belongs to a fruit tree cultivation technology, in particular to a fruit tree pruning method based on branch-leaf-root synchronous growth, which is suitable for a fruit tree with at least three main branches. Different from a traditional strong-removing and strong-remaining method, the method adopts a weak-removing and strong-remaining strategy, and synchronous growth of branches, leaves and roots is realized through the following steps: firstly, performing head removing treatment on a main branch I, reserving 75-85% of length, selectively reserving vigorous branches of more than 1m, and forming inverted branches; in the second year, girdling the inverted branches to promote flower bud formation; after the fruits are harvested in the third year, the fruiting branches are cut off, and the second main branch is subjected to the same treatment; in the fourth year, the first main branches grow new branches again, and the process is repeated. According to the method, the main branches are trimmed and updated in turn, so that continuous growth and fruiting of the trees are kept, the yield and the fruit quality are improved, meanwhile, the influence on the environment is reduced, and the method has important economic and ecological values.

The invention relates to the technical field of planting, and discloses a muskmelon bag type cultivation substrate which comprises a straw mixture, sawdust, plant ash, poultry manure, a nutrient solution and supplementary elements. Wherein the straw mixture comprises one or more of corn straw, wheat straw, soybean straw, sorghum straw and the like; wherein the plant ash is ash generated after grass and tree are burnt; wherein the poultry manure is common poultry and livestock manure of chickens, ducks, pigs and the like. According to the muskmelon bag type cultivation substrate and the application thereof, the survival rate of transplanted muskmelons is high, meanwhile, the manufacturing cost is low, the straw mixture serves as the main raw material, the phenomenon that straw is stacked and placed disorderly can be reduced, sufficient nutrition is provided for transplanted muskmelon seedlings through plant ash, supplementary elements and nutrition of livestock manure, and the yield of the muskmelons is increased. Meanwhile, the roots grow more vigorously, so that the growth cycle is not affected.

The invention relates to the technical field of eucommia ulmoides breeding. The invention provides a breeding method and an identification method of triploid eucommia ulmoides, which comprises the following steps: taking eucommia ulmoides branches, cutting, treating with colchicine solution, and culturing to obtain adventitious buds; cultivating the adventitious buds to obtain seedlings, performing ploidy identification on the seedlings, and screening out tetraploid plants; taking the tetraploid plant as a female parent, hybridizing with the diploid male parent, and harvesting triploid eucommia ulmoides seeds. The invention provides the method for efficiently and stably obtaining the homozygous tetraploid eucommia ulmoides clone, the problems of common chimeras and unstable materials in chemical induction are solved, and reliable parents are provided for cross breeding. A set of complete technical system for directionally creating the triploid eucommia ulmoides by taking tetraploid as a female parent and diploid as a male parent (reverse crossing) is established, and the technical blank from creation of a polyploid parent to breeding of a new triploid variety is filled.

The invention discloses a cubilose fruit grafting seedling raising method, and belongs to the technical field of fruit tree cultivation. The method comprises six aspects of stock planting field establishment, stock seedling raising, stock water and fertilizer management, grafting, grafted seedling water and fertilizer management and grafted seedling field management. A special cutter tool for cubilose fruit grafting is adopted, an L-shaped section can be formed, the section is smooth and good in integrating degree, and the grafting survival rate and the grafting efficiency can be improved. According to the invention, the seedling raising grids are used for stock seedling raising, so that the seedling raising cost can be saved, and the space utilization rate can be improved. According to the method, the thorn-free huanglong and the argentodoxa cuneata are adopted as the stocks, the root systems are developed, the morbidity of root-knot nematodes can be reduced, the grafting survival rate and the growth vigor are improved, and meanwhile the canker resistance of plants is improved; the quality of the rootstock is guaranteed, the grafting growth vigor is improved, bud germination is promoted, and fruit bearing is carried out in advance by optimizing the length, the period and the number of bud points of the rootstock.

This invention relates to the field of daylily cultivation technology, specifically to a method for cultivating daylilies resistant to leaf rust. The invention provides a method for cultivating daylilies resistant to leaf rust, comprising the following steps: Seed treatment: After sterilization, daylily seeds are soaked in a seed treatment agent and then placed at a constant temperature of 35-37℃ for 6-8 days to obtain germinated daylily seeds; Seedling management: The germinated daylily seeds are sown in a seedling substrate and cultivated until germination, obtaining daylily seedlings; Transplanting: The daylily seedlings are transplanted into soil treated with a soil treatment agent. This invention's cultivation method can improve the resistance of daylilies to leaf rust.

The application provides application of Kluyveromyces marxianus ALE-X20 in preparation of milk beer, the Kluyveromyces marxianus ALE-X20 is preserved in the China General Microbiological Culture Collection Center on October 15, 2024, and the preservation number is CGMCC NO.32221. The application domesticates a parent strain Km-P through adaptive laboratory evolution technology, and obtains an evolution strain ALE-X20 with significantly improved lactic acid tolerance. Experiments prove that the strain can grow normally in a 20g / L lactic acid environment, and the phenethyl alcohol yield is 28.2 times that of the parent strain. The strain is applied to milk beer fermentation, so that the number of volatile aroma compounds in the milk beer is increased to 52, the total concentration is increased by 28.18% compared with the parent strain fermentation product, the concentration of alcohol and ester compounds is increased by 33.87% and 32.43% respectively, and the odor activity value of a plurality of characteristic aroma compounds is significantly improved. The application of ALE-X20 in the preparation of milk beer can get rid of the dependence on exogenous essence, realize the production of milk beer with a clean label, and improve the flavor quality and market competitiveness of milk beer.

The invention relates to the technical field of soilless culture, and particularly discloses a plant hydroponic device which comprises a box body with an opening in the top and a hydroponic box with an opening in the top, the hydroponic box is fixedly connected with the inner wall of the box body, and an oxygen supply opening is formed in the side wall of the box body; the cylindrical pipe is arranged in the water culture box, the oxygen supply mechanism is arranged in the cylindrical pipe, the side holes are formed in the water culture box, and the driving assembly is used for driving the cylindrical pipe to rotate. The cylindrical pipe is rotationally connected with the side hole; the oxygen supply mechanism comprises an oxygen supply pipe, an annular hollow pipe, a guide pipe, a movable pipe, a plurality of oxygen supply holes formed in the circumferential direction of the oxygen supply pipe, an oxygen through hole formed in the inner wall of the annular hollow pipe, an auxiliary hole formed in the cylindrical pipe and a power assembly used for driving the movable pipe to reciprocate in the length direction of the guide pipe. The problem that oxygen supply to roots of hydroponic plants is insufficient due to the fact that an existing hydroponic device cannot provide oxygen for the hydroponic plants in time is solved.

The invention relates to the technical field of watermelon cultivation, and particularly discloses a Xinjiang field full-biodegradable mulching film watermelon planting method which comprises the steps and key points of soil land preparation, seed preparation, full-biodegradable mulching film selection, field management, harvesting and the like. In the growth process, vines of watermelons are pressed repeatedly, the number of roots of the watermelons is increased, nutrient absorption is improved, roots and vines are broken, a ventilated and light-transmitting space environment is created, watermelon diseases and insect pests are reduced, the growth period of the watermelons is prolonged by 60 days compared with a conventional method, the stubble number and yield of the watermelons are increased, no chemical fertilizer is applied in the whole process, and the original taste and flavor of the watermelon variety are guaranteed.

The invention relates to the technical field of industrial hemp molecular marker assisted breeding, in particular to a KASP marker associated with the number of cotyledons of industrial hemp and application of the KASP marker. The molecular marker is located on the ninth chromosome of the industrial hemp and is CanS900003 and / or CanS900004; the sequence of the CanS900003 is as shown in SEQ ID NO.1, and the CanS900003 shows remarkable G / A polymorphism at the 201bp position from the 5'end; the sequence of the CanS900004 is as shown in SEQ ID NO. 2, and the CanS900004 shows remarkable A / C polymorphism at the 201bp position from the 5'end of the CanS900004. The KASP molecular marker technology is successfully developed by excavating excellent allelic variation SNP molecular marker sites related to 3-cotyledon cannabis sativa. The application of the technology not only can effectively assist in character improvement selection of industrial cannabis sativa, but also can avoid complicated and time-consuming traditional breeding and screening processes and shorten the breeding cycle, thereby greatly improving the efficiency and accuracy of molecular breeding of industrial cannabis sativa.

The present invention provides a grafting seedling raising method for agarwood with high survival rate and easy to form fragrance, which belongs to the technical field of agarwood seedling raising. The grafting seedling raising method for agarwood with easy to form fragrance of the present invention comprises the following steps: (1) preparing agarwood rootstock; (2) 8 to 12 days before collecting scions, cutting off the 20 to 30 cm portion below the top of the agarwood branch to promote the germination of latent buds of the scion branch, and collecting the scion from the branch with bud eyes; (3) grafting the agarwood rootstock and the scion, and bagging the grafted scion and the grafting site to keep them moist; (4) covering the ground at the root of the grafted rootstock with formulated soil to harden the seedling. Compared with traditional methods, the grafting seedling raising method of the present invention can significantly improve the survival rate, number of buds and length of buds of grafted seedlings, and provide a new guiding idea for breeding excellent strains of agarwood with easy to form fragrance.

The invention discloses a lysimachia christinae Hance variation induction method which comprises the following steps: S1, soaking a lysimachia christinae Hance stem section in an oryzalin solution for mutagenesis treatment; and S2, inoculating the stem segment of the lysimachia christinae after mutagenesis treatment into a culture medium for culture, performing regeneration culture under an illumination condition, and domesticating and transplanting to obtain the triploid lysimachia christinae. The oryzalin is creatively used for inducing the lysimachia christinae hance variation, it is accidentally found that the lysimachia christinae hance variation generates a triploid plant, the breeding efficiency is far higher than that of tetraploid lysimachia christinae hance, the size is large, the ornamental value, stress resistance / adaptability and the like are superior to those of diploid lysimachia christinae hance, and therefore Besides, by further optimizing the concentration and treatment time of the oryzalin and selection of the induced explants, the mutation rate and the triploid rate are remarkably increased and reach nearly 100%, and a new breakthrough point is provided for variety improvement and resistance research of lysimachia christinae hance.

The invention relates to the technical field of gardening and forestry, in particular to an efficient transformation method for an aged peach orchard, which comprises the following steps: simple soil improvement and complementary planting in winter, crown shrinkage and abdication of aged peach trees, rapid trunk cultivation of stocks in spring and side grafting and shaping in summer. The method not only can solve the problems of too high transformation cost and long period of the existing aging peach orchard, but also can effectively solve the problem of continuous cropping obstacles of the peach trees, and realizes current-year field planting, current-year grafting, current-year forming and rapid production in the next year.

This invention discloses an engineered astaxanthin-producing probiotic yeast strain, its construction method, and its applications, belonging to the field of synthetic biology. The engineered strain uses β-carotene-producing *Saccharomyces cerevisiae* as the substrate cell. Its genome integrates a fusion gene selected through adaptability screening. This gene is composed of *BDC263crtW* from *Saccharomyces cerevisiae*, a flexible linker peptide, and *AspcrtZ* from *Alcaligenes aeruginosa*, linked sequentially. Simultaneously, based on systematic expression screening of multiple key genes, the endogenous acetyl-CoA synthesis key gene ACS1 was optimized and overexpressed, while the acetic acid metabolism negative regulatory gene YPL062W was knocked out. The construction method includes plasmid construction of the aforementioned fusion expression module and ACS1 expression module, and targeted integration into the genome. This invention significantly improves astaxanthin synthesis levels and enhances the engineered strain's tolerance to the simulated gastrointestinal environment and its antioxidant capacity through enzyme source optimization and substrate channel effects. This engineered strain can be directly used as a functional live bacteria preparation for food and feed additives, with broad prospects for industrial application.

The invention relates to the technical field of cultivation equipment, and discloses a bitter gourd rice planting rice seed auxiliary cultivation box which comprises a box body as a basic component of the whole device and used for assembling and bearing all processing mechanisms and subordinate lower structural parts of the processing mechanisms. And the natural simulation mechanism is arranged at the upper part of the box body and is used for simulating light, natural wind and natural rainfall environmental conditions in an external environment in a rice seed cultivation process. By adding and arranging the auxiliary stimulation mechanism, in the rice seed germination accelerating stage, the mechanism generates an alternating electromagnetic field through electrification of an electromagnetic coil, the activity of various enzymes in seeds can be effectively activated, sufficient energy and nutrition are provided for seed germination, the germination accelerating period is greatly shortened, and the germination efficiency and quality are improved; the electromagnetic field generated by the mechanism can accurately stimulate division and growth of root system cells, promote the root system to be more developed and robust, enhance the ability of the root system to absorb nutrients and moisture in soil, and enable rice seedlings to grow more robust.

The invention discloses a breeding method of a high-quality, super-high-yield and high-antioxidant functional purple potato new strain. Belongs to the technical field of heterosis utilization. The breeding method comprises the following steps: hybridizing oblong purple-peel and purple-pulp potatoes serving as female parents and oval red-peel and red-pulp potatoes serving as male parents, breaking dormancy of obtained G0 seeds, and performing generation-adding treatment in an artificial climate environment to obtain F1 seed potatoes; the method comprises the following steps: sending a single F1 seed potato block to regions with different ecological conditions, carrying out shuttle breeding planting, field planting and observation selection, carrying out stem tip detoxification on the obtained F2 seed potato, carrying out field planting and observation selection, carrying out field planting and screening on the obtained F3 seed potato, and thus obtaining the high-quality, super-high-yield and high-antioxidant functional purple potato new strain. According to the new purple potato strain, two excellent genes of the big potatoes and the multiple potatoes are well integrated in the same individual, and the multi-resistance genes are perfectly combined, so that high-yield and high-quality colored potatoes are obtained.

The invention discloses a patient-derived gastrointestinal stromal tumor organoid culture medium based on an SCF functional nucleic acid simulant and application of the patient-derived gastrointestinal stromal tumor organoid culture medium, and belongs to the technical field of tumor organoid culture. The patient-derived gastrointestinal stromal tumor organ culture medium based on the SCF functional nucleic acid simulant can effectively improve the nutrient composition proportion of the gastrointestinal stromal tumor organ culture medium, supports rapid growth and self-assembly of gastrointestinal stromal tumor organs, promotes tissue fibroblasts to be converted into tumor-related fibroblasts, and improves the survival rate of the tumor-related fibroblasts. The tumor microenvironment can be highly simulated, the gastrointestinal stromal tumor organ with the diameter of more than 300 microns and vigorous growth vigor can be obtained within 8-12 days, and long-term subculture can be carried out; cultured gastrointestinal stromal tumor organs are highly consistent with tumor tissue pathological phenotypes and histochemical characteristics of patients from the organs, and the method can be used for clinical drug sensitivity assessment and basic scientific research of gastrointestinal stromal tumors; and the culture medium is better in stability and lower in cost.

The invention discloses lactobacillus gasseri VB247 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on February 20, 2023, and the preservation number of the lactobacillus gasseri VB247 is CGMCC No. 26575. The lactobacillus gasseri VB247 can obviously improve the expression level of AQP2mRNA in female cervix and vagina epithelial cells, and can be used for preventing and / or relieving and / or treating vagina lubrication disorder caused by perimenopausal period, premature ovarian failure and the like; in addition, the lactobacillus gasseri VB247 disclosed by the invention has a huge application prospect in preparation of products such as foods, health-care foods, medicines, hygienic products and the like.

The invention discloses an isolation device for forest genetic breeding, which comprises a greenhouse main body, one side of the greenhouse main body is provided with a greenhouse outer wall, the top of the greenhouse main body is provided with a motor shell A, the outer wall of the greenhouse outer wall is connected with a water inlet, and the front end of the greenhouse main body is provided with a zipper door. A protective net and a fan are arranged below the water inlet, a supporting frame is fixedly installed in the greenhouse body, an internal water pipe is arranged below the supporting frame, a cultivation device and a protective fence are arranged below the internal water pipe, a variable frequency motor is fixedly installed in a motor shell A, and a motor is fixedly installed in a motor shell B; one end of the variable frequency motor is connected with a rotating shaft, and the outer side of the rotating shaft is connected with a sunscreen film.According to the device, the fan can be driven to rotate through rotation of the driving motor, so that air in the greenhouse can be driven to flow, and fresh air is brought to the interior of the greenhouse.