The invention relates to a preparation method of a Hypsizigus marmoreus cultivation material. The Hypsizigus marmoreus cultivation material is prepared by the following components by the weight percent: 32-45 of wood flour, 25-40 of mill offals, 12-19 of corn cob, 3.6-6.7 of corn meal, 3.5-4 of rice bran meal, 0.5-1 of wheat straw meal, 1.7-2 of white sugar, 0.5-1 of lime, 0.1-0.3 of gypsum, 0.1-0.3 of superphosphoric calcium carbonate, 0.1-0.3 of brown sugar, and 0.5-0.8 of magnesium sulfate. The wood flour, the mill offals, the corn cob, the corn meal, the rice bran meal, the wheat straw meal and the white sugar are combined together according to the weight percent to be put into a crushing machine to be crushed into mixture with 120 meshes of particles, the mixture is taken out, the lime, the gypsum, the superphosphoric calcium carbonate, the brown sugar and the magnesium sulfate are added to the mixture in proportion, all the new mixture is poured into a stirring machine to be stirred for 65 minutes and then taken out, water is added to the mixture according to the proportion of 1:1.3-1:1.5, and the mixture is blended and then put in a fermenting tank to be piled in stacks, covered with plastic cloth, compacted and fermented for 68 hours to obtain the qualified cultivation material.