5results about How to "Strong growth" patented technology

The invention relates to the technical field of eucommia ulmoides breeding. The invention provides a breeding method and an identification method of triploid eucommia ulmoides, which comprises the following steps: taking eucommia ulmoides branches, cutting, treating with colchicine solution, and culturing to obtain adventitious buds; cultivating the adventitious buds to obtain seedlings, performing ploidy identification on the seedlings, and screening out tetraploid plants; taking the tetraploid plant as a female parent, hybridizing with the diploid male parent, and harvesting triploid eucommia ulmoides seeds. The invention provides the method for efficiently and stably obtaining the homozygous tetraploid eucommia ulmoides clone, the problems of common chimeras and unstable materials in chemical induction are solved, and reliable parents are provided for cross breeding. A set of complete technical system for directionally creating the triploid eucommia ulmoides by taking tetraploid as a female parent and diploid as a male parent (reverse crossing) is established, and the technical blank from creation of a polyploid parent to breeding of a new triploid variety is filled.

The invention discloses a cubilose fruit grafting seedling raising method, and belongs to the technical field of fruit tree cultivation. The method comprises six aspects of stock planting field establishment, stock seedling raising, stock water and fertilizer management, grafting, grafted seedling water and fertilizer management and grafted seedling field management. A special cutter tool for cubilose fruit grafting is adopted, an L-shaped section can be formed, the section is smooth and good in integrating degree, and the grafting survival rate and the grafting efficiency can be improved. According to the invention, the seedling raising grids are used for stock seedling raising, so that the seedling raising cost can be saved, and the space utilization rate can be improved. According to the method, the thorn-free huanglong and the argentodoxa cuneata are adopted as the stocks, the root systems are developed, the morbidity of root-knot nematodes can be reduced, the grafting survival rate and the growth vigor are improved, and meanwhile the canker resistance of plants is improved; the quality of the rootstock is guaranteed, the grafting growth vigor is improved, bud germination is promoted, and fruit bearing is carried out in advance by optimizing the length, the period and the number of bud points of the rootstock.

This invention relates to the field of daylily cultivation technology, specifically to a method for cultivating daylilies resistant to leaf rust. The invention provides a method for cultivating daylilies resistant to leaf rust, comprising the following steps: Seed treatment: After sterilization, daylily seeds are soaked in a seed treatment agent and then placed at a constant temperature of 35-37℃ for 6-8 days to obtain germinated daylily seeds; Seedling management: The germinated daylily seeds are sown in a seedling substrate and cultivated until germination, obtaining daylily seedlings; Transplanting: The daylily seedlings are transplanted into soil treated with a soil treatment agent. This invention's cultivation method can improve the resistance of daylilies to leaf rust.

The application provides application of Kluyveromyces marxianus ALE-X20 in preparation of milk beer, the Kluyveromyces marxianus ALE-X20 is preserved in the China General Microbiological Culture Collection Center on October 15, 2024, and the preservation number is CGMCC NO.32221. The application domesticates a parent strain Km-P through adaptive laboratory evolution technology, and obtains an evolution strain ALE-X20 with significantly improved lactic acid tolerance. Experiments prove that the strain can grow normally in a 20g / L lactic acid environment, and the phenethyl alcohol yield is 28.2 times that of the parent strain. The strain is applied to milk beer fermentation, so that the number of volatile aroma compounds in the milk beer is increased to 52, the total concentration is increased by 28.18% compared with the parent strain fermentation product, the concentration of alcohol and ester compounds is increased by 33.87% and 32.43% respectively, and the odor activity value of a plurality of characteristic aroma compounds is significantly improved. The application of ALE-X20 in the preparation of milk beer can get rid of the dependence on exogenous essence, realize the production of milk beer with a clean label, and improve the flavor quality and market competitiveness of milk beer.

This invention discloses an engineered astaxanthin-producing probiotic yeast strain, its construction method, and its applications, belonging to the field of synthetic biology. The engineered strain uses β-carotene-producing *Saccharomyces cerevisiae* as the substrate cell. Its genome integrates a fusion gene selected through adaptability screening. This gene is composed of *BDC263crtW* from *Saccharomyces cerevisiae*, a flexible linker peptide, and *AspcrtZ* from *Alcaligenes aeruginosa*, linked sequentially. Simultaneously, based on systematic expression screening of multiple key genes, the endogenous acetyl-CoA synthesis key gene ACS1 was optimized and overexpressed, while the acetic acid metabolism negative regulatory gene YPL062W was knocked out. The construction method includes plasmid construction of the aforementioned fusion expression module and ACS1 expression module, and targeted integration into the genome. This invention significantly improves astaxanthin synthesis levels and enhances the engineered strain's tolerance to the simulated gastrointestinal environment and its antioxidant capacity through enzyme source optimization and substrate channel effects. This engineered strain can be directly used as a functional live bacteria preparation for food and feed additives, with broad prospects for industrial application.