The invention relates to a fertilization method for preventing and controlling premature senescence of honey peach trees, the fertilization method comprises the following steps: 1, before leaves of the honey peach trees are fallen, a biological bacterial fertilizer, a compound fertilizer, calcium superphosphate and No. 1 microbial bacterial liquid are applied to roots; 2, before germination stage,No. 2 microbial bacterial liquid is applied to the roots; 3, before flowering, urea and a boron fertilizer are applied to the roots; 4, during fruit setting stage, No. 1 water soluble fertilizer andNo. 3 microbial bacterial solution are applied to the roots, and foliar fertilizer 1 is sprayed simultaneously; 5, in fruit swelling stage, water-soluble fertilizer 2 is applied to the roots; and 6, in fruit color turning stage, microbial bacterial solution 4 is applied to the roots, and foliar fertilizer 2 is sprayed simultaneously. The fertilization method can comprehensively delay the prematuresenescence of the peach trees from the aspects of improving soil of peach orchards, preventing and controlling peach tree gummosis disease, supplementing nutrients, and improving tree vigor. At the same time, the biological control of the peach tree gummosis disease and other diseases can be realized by applying microbial agents, the number of medications used throughout a year can be reduced by1-2 times, environmental pollution is reduced, and the quality and flavor of honey peaches can be improved.