49 results about "Reciprocal cross" patented technology

The invention relates to a set of chloroplast SNP and INDEL molecular marker combination for maternal traceability of maize. On the basis of high quality resequencing data, the invention develops a set of maize chloroplast genome polymorphic locus combination, the polymorphic loci are CPM001-CPM100, which include 96 SNP loci and 4 IDNEL loci. The 100 chloroplast loci can be applied to construction of a maize chloroplast DNA fingerprint database, parental traceability, and reciprocal cross identification. Through application of the chloroplast polymorphic loci, the range of available marker loci for maize on the genomic level is expanded; and a new idea and method are provided for maize varieties, germplasm resource identification, genetic relationship evaluation, cytoplasmic genetic characteristics and other studies.

The invention belongs to the field of establishment of a population genetic resource bank, and provides a scientific establishment method of a goat resource population bank, and provides a goat reference family system establishment case, and relates to two national-level genetic resource varieties, namely, a Dazu black goat and an Inner Mongolia Cashmere goat (Alxa type). According to the resource population establishment method, forward cross and reciprocal cross of Dazu black goat and Inner Mongolia Cashmere goat (Alxa type) are utilized, random mating of genetic relationship is avoided for F1, so that Mendel genetic characters are separated, a goat economic character QTL or a functional gene is located by virtue of a genome means, and a relatively-precise genetic mark is provided for the goat breeding. The genetic resource bank comprises goat population, a genealogy inforamtion database, a phenotypic character database, a DNA sample library, an RNA sample library and a genetic variation information database.

The invention provides molecular markerd of a maize chloroplast genome and its application in cultivar identification. The molecular markerd comprise 57 SNP markers and 6 InDel markers. The 63 molecular markers and primers developed according to the markers can be used in any one of the following: (1) constructing a corn variety chloroplast DNA-SNP and InDel fingerprint database, (2) carrying outcorn maternal line traceability analysis, and (3) performing reciprocal cross identification on corn samples. Through the application of the chloroplast polymorphic primer composition, the range of available marker sites of the corn is expanded at the genome level and the new ideas and technical means are provided for the study of maize variety and germplasm resource identification, genetic relationship evaluation and cytoplasmic genetic characteristics.

The invention discloses an undersized white feather broiler breeding method. An undersized yellow chicken and a white feather broiler are used for being in reciprocal cross and after that the yellow chicken and the white feather broiler are in horizontal cross fixing. Then, shutting breeding can be proceeded to breed a breed of undersized white feather broilers. The undersized white feather broiler breeding method is used for breaking through a traditional thought that a large-sized cock is mated with a large-sized hen to give birth to a parents-generation white chicken in a normal bodily form in the prior art. An undersized cock is chosen to mate the large-sized hen to give birth to the parents-generation white chicken in a grain-saving type. A method that an undersized yellow chicken (contains a dw gene) is half-bred with a white fast-growing chicken is used for choosing and breeding a breed of dominance undersized white chickens. High isozygoty and high stability of the dw gene can be guaranteed, and absolute security of resource can be guaranteed. An adult chicken is shapely in body and gesture, rounded in bodily form, huge in head, thick in neck, compact and shining in feathers and strong and muscular in chest-leg muscles. Chicken herd is good in uniformity and strong in disease resistance. Hens which enter into pheasantries for 66 weeks can lay 170-175 eggs. The weight of each egg is 50-60 grams. Passing percent of hatching egg is more than 95%. Emergence rate of hatching is 90%.

The invention relates to a molecule marking method for a red peel gene of weed rice and belongs to the field of molecular genetics. A rice type of 02428(white peel) is utilized to be hybridized with Yangzhong weed rice (red peel) to confirm that the red peel gene of the Yangzhong weed rice is a dominance single gene which is decided by a maternal gene; a reciprocal cross result shows to have no cytoplasm effects. An InDel marker RID14 is designed according to the gene structure of the red peel gene Rc of the cloned common wild rice; the InDel marker is co-separated with the red peel gene of the weed rice. The marker can be utilized to accurately and effectively identify the red weed rice and the derived line thereof from the cultivated rice and can provide a molecule basis for the identifying of the weed rice.

The invention relates to a seed production method by interspecific crossing of green abalone and Pacific abalone and relates to a hybrid seed production method for aquatic shellfishes. Pacific abalone and green abalone are used as parent abalones. Sea water temperature is controlled to 24 DEG C-26 DEG C through a temperature control facility to allow for synchronous accelerated ripening of gonads of the parent abalones. The male abalones and female abalones are placed in different culture ponds for breeding. Sex character of the parent abalones in each culture pond needs to be equal. The individuals with maturely developed gonads are induced for spawning by drying in the shape, ultraviolet irradiation and sea water stimulation until the female and male abalones lay eggs and discharge semen. Insemination is allowed at the water temperature of 20 DEG C to 22 DEG C. Inseminated hybrid abalone fry are obtained by direct cross combination, namely female Pacific abalone with green abalone, or by reciprocal cross combination, namely female abalone with Pacific abalone. The inseminated hybrid abalone fry are bred by conventional larva, offspring and intermediate breeding methods, and direct-cross or reciprocal-cross fry of green abalone and Pacific abalone are obtained finally.

The invention relates to five autosexing mating methods for laying quails, which respectively comprise: (1) F1 generation male quails produced by a yellow-feather male quail and a black-feather female quail have incomplete black feather, and female quails have light gray feather; (2) F1 generation male quails produced by a white-feather male quail and a black-feather female quail have incomplete black feather, and female quails have white feather; (3) F1 generation male quails produced by a gray-feather male quail and a black-feather female quail have black feather, and female quails have gray feather; (4) F1 generation male quails produced by a gray-feather male quail and a white-feather female quail have incomplete black feather, and female quails have light gray feather; (5) and F1 generation male quails produced by a white-feather male quail and a gray-feather female quail have incomplete black feather, and female quails have white feather. The autosexing precision of the method reaches 100%; the method can carry out the autosexing on the gray-feather quails and the Beijing white-feather quails in case of cross breeding and reciprocal cross; the method can automatically sex the quails immediately after birth, thereby simplifying the sexing means, reducing the cost and enhancing the efficiency; and the invention fully utilizes the genetic resources of quails in China and enhances the production efficiency of the quail hybrids.

The invention discloses a hybrid breeding and cultivation method for cabbage. The method comprises the following steps: 1) parental selection: selecting at least Liangqing cabbage and Xiaoza 56; 2) parental selfing and separating: separating the progeny from the selfing process of Liangqing cabbage and selecting a good individual A, and separating the progeny from the selfing process of Xiaoza 56and selecting a good individual B; 3) parental selfing and purifying: selfing and purifying the individual A for five generations, and selecting antiviral and downy mildew resistant and soft rot resistant self-incompatible lines with consistent traits as progeny C; selfing and purifying the individual B for five generations, and selecting antiviral and downy mildew resistant self-incompatible lines as progeny D; 4) hybridizing: performing a reciprocal cross compatibility test with progeny generations C and D, and performing hybridization with generation C as female parent and generation D as male parent, to obtain the cabbage seed. The technical solution provided by the invention solves the problem that the cabbage species currently introduced to Yunnan, Guizhou, Sichuan have poor qualityand low yield when being planted in spring and autumn.

The invention discloses a selection method of Taihu pigs and belongs to the field of animal breeding. Reciprocal cross is performed on measly pigs, Meishan pigs, Fengjing pigs and Erhualian pigs from the TaiHu lake area, thus heterosis is expanded, a breeding line of Meishan pig-measly pig-Erhualian pig-Fengjing pig is established, and a breed which is high in reproductive performance and stable in heritability is selected. Compared with the Taihu pigs without hybrid cross such as Meishan pigs, the Taihu pigs subjected to the reciprocal cross are high in total number born and number born alive, the defect brought by selfing of the Taihu pigs is overcome, the heritability of the reproductive performance of local pigs in China is maintained, and a special high-quality female parent specialized line is provided for pig production and breeding industry.

The invention discloses a selfing line SSR (Simple Sequence Repeat) and phenotype information based corn hybrid combination method and system. The method comprises: performing standardization treatment on preset selfing line field identification data; for the selfing line field identification data subjected to the standardization treatment, determining a value of a preset characteristic in each selfing line, and according to the value of the preset characteristic, calculating a phenotype distance of a selected hybrid line; according to the phenotype distance, determining the defect degree and superiority degree of the preset selfing line field identification data; according to the preset selfing line SSR molecular marker data, determining a value of a preset index in each selfing line; according to the value of the preset index, determining the selfing line meeting a preset condition to be subjected to hybrid combination; calculating a genetic distance of the selected hybrid line; according to the phenotype distance and the genetic distance, establishing a heterotic rate index of selected parents; calculating a reciprocal cross value SV; and according to a biparental heterotic rate MD, a mean value, defined in the specification, of biparental comprehensive values and the reciprocal cross value SV, obtaining a hybrid combination value HV.

The invention provides a breeding method capable of improving a white feather rate and class of semi-muscovy ducks. Male and female parent samples of the semi-muscovy duck are systematically bred by mainly using reciprocal cross, self cross and subculture selection methods. The breeding method has the advantages that the white feather rate and class A of the semi-muscovy ducks can be efficiently and systematically improved, the white feather quality of the semi-muscovy duck is comprehensively improved, the high-quality and first-class feather and the white and attractive carcass are provided for market, the market competitiveness and farming benefit of the semi-muscovy duck are increased, and the method is favorable for promoting the white-feather semi-muscovy duck industry to be developed to a higher level.

The invention relates to a breeding method of a pinctada martensi black-shell strain for sea pearl cultivation and belongs to the technical field of pearl breeding. The breeding method includes taking two geographical populations, namely the wild population of Lingshui of Hainan and the cultured population of Leizhou of Beibuwan as the breeding base populations, selecting high-quality individualities as crossing parents from the two populations , breeding progeny by means of reciprocal cross and selfing of different geographical populations, and selecting the progeny of the combination of 'leizhou *lingshui' with remarkable growth superiority from the progeny to perform enclosed breeding of population so as to obtain a certain amount of the pinctada martensi black-shell strain with obvious feature of black shells, good economic character and stable heritability. The breeding method has the advantages of feasible principle, simple processes and low production cost, can improve the survival rate, growth rate, pearl quality and output of the pinctada martensi greatly, and accordingly can improve sustainable development of the national sea pearl cultivation field.

The invention discloses a method for breeding new varieties of black pigs in Southern Henan Province comprising following steps: Duroc pigs are hybridized with Huainan pigs to breed a F1 generation Du-Huai hybrid pig herd; Duroc pigs are hybridized with the F1 generation Du-Huai hybrid pig herd to obtain a F2 generation Du-Du-Huai hybrid pig herd; reciprocal cross is performed between the F1 generation Du-Huai hybrid pig herd and the F2 generation Du-Du-Huai hybrid pig herd to breed a base pig herd; ideal boar and sow individuals are selected from the base pig herd for cross hybridizing fixing to breed zero generation breeding core herds; herd continuous progeny selection is performed on zero generation breeding core herds and new varieties are obtained by expanded breeding. The method for breeding a new variety of high quality pigs which are improved Huainan pigs with 62.5% foreign blood is first brought forward; the high quality new variety pigs which keep good characteristics of local Huainan pigs and absorb production performance of foreign Duroc pigs are bred.

The invention discloses a crossbreeding method of hippocampus kelloggi and hippocampus trimaculatus. The crossbreeding method comprises the following steps that S1, parents of the hippocampus kelloggi and the hippocampus trimaculatus are selected; S2, strengthening breeding is carried out on the parents of the hippocampus kelloggi and the hippocampus trimaculatus in S1; S3, direct cross and reciprocal cross are carried out on the hippocampus parents in S2; S4, meticulous breeding and parent and baby hippocampus management are carried out on the pregnant parents in S3; S5, produced fry in S4 are bred in similar environment according the same breeding mode; S6, weight and survival rate comparison is carried out on the fry at the month ages of one, two and four, and a high-quality crossbreeding hippocampus germplasm strain with the high survival rate and genetic stability is obtained. The survival rate of the crossbred progeny obtained through the method is greatly improved relative to that of self-bred progeny, a foundation can be provided for breeding a hippocampus disease resisting strain, and development of the hippocampus culture industry is promoted.

The invention discloses a production method for gray feather meat ducks. The method comprises the steps that 1, Liancheng white ducks and Cherry Valley ducks (SM3) are selected for direct cross, the character is fixed through athwart cross, an F series is obtained, the Liancheng white ducks and the Cherry Valley ducks (SM3) are subjected to reciprocal cross, the character is fixed through athwart cross, and an M series is obtained; 2, an F series breeding family and an M series breeding family are established, and the F series and the M series are reasonably selected and matched through a family selection and individual selection combining method; 3, the F series serves as a male parent, the M series serves as a female parent, and an F1 generation is obtained; 4, the F1 generation serves as the female parent to be backcrossed with the Cherry Valley ducks (SM3), and obtained offsprings are the gray feather meat ducks. On the basis of guaranteeing the advantages of local ducks, the growth speed is increased, the feed conversion ratio is reduced, the carcass weight is increased, and the dressing percentage is increased.

The invention discloses a method for cultivating recessive white feather pigeons, and the method comprises the following steps: (1) performing selfing of colored feather pigeons A, judging the feathercolor of offsprings, and selecting and remaining pure white individuals B and individuals C which are consistent in the feather color with the parent; (2) performing selfing of the B colony, and after breeding for half a year, if the offsprings have non-pure white feather color, eliminating all the offsprings and parent pigeons; (3) performing reciprocal cross of the B colony and the C colony, and after breeding for half a year, if the offsprings have non-pure colored feather color, eliminating all the parent pigeons; and (4) performing selfing of the B colony of the parent pigeons selected and remained by the step (3), after breeding for 6-10 months, judging the feather color of the offsprings, and if the feather color of the offsprings is not consistent with that of the parent pigeons,eliminating all the offsprings and parent pigeons to obtain the B colony as the recessive white feather pigeons.

The invention provides a set of maize chloroplast InDel markers applicable to a capillary electrophoresis detection platform, totally including six InDel molecular markers, and the invention also provides a primer composition and a detection method of the six InDel molecular markers applicable to the capillary electrophoresis detection platform. With the application of the six InDel molecular markers, (1) a fingerprint database of the maize variety chloroplast InDel markers can be constructed, (2) maternal line tracing analysis of a maize sample can be implemented, and (3) reciprocal cross identification of the maize sample can be implemented. According to chloroplast InDel polymorphic sites and the application of the primers, the scope of available marker sites of maize can be expanded ina genome level; therefore, a new thinking and a new method are provided for researches on maize varieties, germplasm identification, genetic relationship evaluation, cytoplasmic inheritance characters and the like.

The invention discloses a premature, disease-resistant and stress-resistant mini broccoli breeding method and belongs to the technical field of plant cultivation. Flower buds, suitable for microspore culture, of broccoli and cabbage mustard breeding materials from the mid-late uninucleate stage to the early dicaryon stage are used for microspore culture, a large number of regeneration plants are created, excellent DH plants are screened out for selfing, a DH strain stable in botanical character, good in combining ability and large in pollen quantity is obtained, and hybridization first generation seeds F1 are prepared in the flowering stage through artificial emasculationare carried out to obtain; generation-adding is conducted on the F1, then good single plants with botanical characters similar to those of broccoli are selected, selfing in the bud stage is carried out to obtain F2, then generation-adding is conducted on three generations to select self-incompatible strains 11-13-7-8-3 and 12-15-9-3-2 which are excellent and stable and cultivated through continuous selfing in the bud stage, and premature, disease-resistant and stress-resistant mini broccoli is obtained through hybridization. The self-incompatible strains are used for mating, reciprocal cross plants are consistent in character, and the yield of produced seeds is increased by over 50% compared with that of sterile line seed production.

The invention discloses a breeding method for loose-flower-ball cauliflower with green stalks. The method comprises the following steps: (1) collecting different types of loose-flower-ball cauliflowerresources, performing selfing in a blooming period, and selecting a self-incompatible single plant with a self-compatible index of less than 2.0, loose flower balls, and dark green flower stalks; (2)continuing to select a single plant which meets a target character in the self-incompatible single plant, performing selfing for 5-6 generations through stripping buds, and selecting a stable self-incompatible line with a self-compatible index of less than 0.5 in a blooming period, and a self-compatible index of more than 10.0 in a bud stage; and (3) selecting two parents with complementary genetic characters from the stable self-incompatible line, performing pollination on flowers through reciprocal cross, selecting positive and negative hybridization combinations with cross-compatible indexes of more than 8.0 in a blooming period, and reserving seeds. According to the invention, the two parents are both double-harvest seeds prepared from self-incompatible lines, thus genetic complementarity is high, hybridization advantages are obvious, adaptability is strong, seeds produced by the two parents can be harvested and utilized, and different market demands can be met at the same time.

The invention discloses an environment-friendly single liquid aqueous mixed coating which comprises an agent A: an aqueous resin dispersion liquid and a selected bridging agent as an agent B, whereinthe aqueous resin dispersion liquid comprises a group composed of tertiary amine (for example aqueous resin neutralized by triethylamine) and at least one aqueous resin which is selected from water-borne epoxy resin, water-borne polyurethane and water-borne acrylic-based resin, wherein the at least one aqueous solution contains carboxyl and other reactive functional groups; when the agents A and Bare mixed to form the single liquid aqueous mixed coating, sprayed and dried, the bridging agent is bridged with the at least one aqueous resin to form a reciprocal crossed netty structure. The single liquid aqueous mixed coating can be coated to various materials to form non-permanent material protecting films. After the materials are processed, the materials are only soaked in water, and the protecting films can be peeled, recovered and centrally processed from the surfaces of the materials easily without using any organic solvents in the full course. The invention also discloses a double liquid aqueous mixed coating.

The invention discloses a method for producing seeds of spotted goose seedlings. The method comprises the following steps: (1) performing self-group propagation on breeding geese with spotted feathercharacteristics; (2) establishing a white feather group for white feather mutation male and female geese appearing in self-group propagation offspring, and performing family breeding combined with individual breeding to breed a white feather high-yield line; (3) performing reciprocal cross of spotted feathers and the white feather mutation male and female geese, and screening out spotted feather homozygous individuals according to the separation of the feather color of the offspring; (4) establishing a spotted feather group for the spotted feather homozygous male and female geese, and performing family breeding combined with individual breeding to breed a spotted feather fast-growing line; and (5) performing cross utilization on the bred spotted feather fast-growing line male geese and thewhite feather high-yield line female geese to produce the spotted feather goose seedlings. Compared with the prior art, the method disclosed by the invention can realize the 100% of the spotted rateof the offspring, fully utilizes the advantage of high reproductive performance of the maternal line, significantly reduces the production cost of the goose seedlings, can meet the market demand for the spotted goose seedlings, and has good potential large-scale application prospects.