Common intertidal shellfish polymerization hybridization breeding method

A technology for shellfish and tidal flats, which is applied in the breeding field of aggregation and hybridization of common tidal flat shellfish, which can solve the problems of poor stress resistance and slow growth speed, and achieve the effects of strong stress resistance, increased growth speed, and enriched gene composition

Active Publication Date: 2009-02-11
DALIAN FISHERIES UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Finally, it can solve the problems of poor stress resistance and slow growth rate of the current tidal flat shellfish germplasm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The first step is to collect parents of Philippine clams from different geographical groups: In 2005, 2-year-old healthy individuals were selected as parents. The parents were from Putian in southern China, Dalian in northern China, Busan in South Korea, and Tokyo in Japan.

[0022] The second step, the ecological maturation promotion of clam parents of different geographical groups: the collected parents were placed in the shrimp ponds of Zhuanghe Ocean Village for ecological maturation promotion. Mature in sync.

[0023] The third step, the binary positive and negative hybridization of the populations of North and South China, Korea and Japan: Use an electric drill with a diameter of 0.6mm to identify the gender of the parents. After identification; after being stimulated by drying in the shade for 8 hours and running water for 1 hour, the parents can simultaneously lay eggs and ejaculate; Then follow South China♀×North China , Northern China♀×Southern China ;Kore...

Embodiment 2

[0028] The first step is to collect parents of Philippine clams from different geographical groups: In 2005, 2-year-old healthy individuals were selected as parents. The parents were from Putian in southern China, Dalian in northern China, Busan in South Korea, and Tokyo in Japan.

[0029] The second step, ecological maturation promotion of clam parents of different geographical groups: the collected parents were placed in the tidal flats of Zhuanghe Ocean Village for ecological maturation promotion. After 6-12 months of bottom sowing breeding, the gonads were synchronized in the autumn of 2006 Mature.

[0030] The third step, the binary positive and negative hybridization of the populations of North and South China, Korea and Japan: Use an electric drill with a diameter of 0.6mm to identify the gender of the parents. After identification; after being stimulated by drying in the shade for 8 hours and running water for 1 hour, the parents can simultaneously lay eggs and ejaculat...

Embodiment 3

[0035] The first step is to collect parents of Philippine clams from different geographical groups: In 2005, 2-year-old healthy individuals were selected as parents. The parents were from Putian in southern China, Dalian in northern China, Busan in South Korea, and Tokyo in Japan.

[0036] The second step, the ecological maturation promotion of clam parents of different geographical groups: the collected parents were placed in the shrimp ponds of Zhuanghe Ocean Village for ecological maturation promotion. Mature in sync.

[0037] The third step is the binary positive and negative hybridization of the populations of North and South China, Korea and Japan: through the stimulation of 8 hours of shade drying and 1 hour of running water, the parents can simultaneously lay eggs and ovulate; during the process of ovulation and ovulation, male and female can be distinguished, and the The male and female individuals were captured separately; and then according to South China ♀×North Ch...

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Abstract

The invention discloses a breeding method of common mudflat shellfish by convergent cross. The method comprises the steps: mudflat shellfish parents from more than four different geographic populations are collected; the shellfish parents of different geographic populations are carried out eco-maturing; then dual reciprocal cross is carried between two geographic populations; a first filial generation of dual reciprocal cross combination is bred up by a three-stage method; then the convergent cross of the first filial generation of dual reciprocal cross is carried out so as to obtain a first filial generation of convergent cross; and the above steps are repeated on the basis thereof till the filial generation of convergent cross accumulates the good genes of all the geographic populations. The method overcomes the geographic isolation and the biological differences of sexual maturity and breeding among different geographic populations. Compared with the existing mudflat shellfish varieties, the shellfish integrates the good genes from more than four different geographic populations; the heterozygosity is improved greatly, thus causing the converged half-bred individual to present characteristics of fast growing and high stress resistance.

Description

technical field [0001] The invention relates to a genetic breeding technology of shellfish, in particular to a breeding method for aggregation and hybridization of common beach shellfish. Background technique [0002] Most of the common tidal flat shellfish are widely distributed cultured species, which have the characteristics of short breeding cycle, low investment and high income. At present, in tidal flat shellfish breeding, there are some unfavorable phenomena such as unclear basic status of germplasm resources, quality decline, poor stress resistance, individual miniaturization, and unit yield reduction. Moreover, the breeding objects are all wild types, and there are almost no artificially bred high-yielding, stress-resistant varieties. It is an important way to solve these problems by genetically improving tidal flat shellfish and cultivating new species with fast growth and strong stress resistance (Yan Xiwu, 2005). [0003] Selective breeding is an effective meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 闫喜武张跃环霍忠明杨凤张国范王琦金晶宇
Owner DALIAN FISHERIES UNIVERSITY
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