38results about How to "High heterozygosity" patented technology

The invention relates to a Hi-C high-throughput sequencing and database building method for eukaryote DNA. According to the DNA-cell-nucleuse intramolecular cyclization step, end-repaired DNA and a cyclizing system are mixed, incubated for 2 h-4 h at the temperature of 4 DEG C to 6 DEG C and then incubated for 4 h-6 h at the temperature of 16 DEG C to 22 DEG C, and materials obtained after intramolecular cyclization are obtained, wherein the ingredients of the cyclizing system comprise T4 ligase damping liquid, BSA, T4 DNA ligase and at least one polyethylene glycol selected from PEG 4000 and PEG 6000. By means of the Hi-C high-throughput sequencing and database building method, the character cyclizing efficiency of DNA can be effectively improved, and the effective data rate obviously higher than that of a traditional method is obtained.

The present invention discloses a new short nucleotide tandem repeat locus and its application, the short tandem repeat locus G7S0005, the sequence is shown in SEQ ID NO.: 1, used to prepare (a) for genetic relationship Reagents or kits for analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity testing or blood relationship analysis; (d) for detecting whether there is maternal blood in the extracted amniotic fluid Contaminated reagents or kits; and / or (e) kits for detecting whether leukocytes in recipients have been replaced by donor cells after bone marrow transplantation. The short tandem repeat locus G7S0005 has a high degree of discrimination and can effectively analyze genetic relationships.

The invention discloses a method for identifying and dividing Hu sheep families by using microsatellites, and applications thereof. The method comprises the following steps: extracting Hu sheep genomeDNA; selecting a Hu sheep polymorphism microsatellite marker, and modifying with a fluorophore; carrying out landing type PCR amplification; and analyzing data such as genotypes and the like, identifying and dividing families, and carrying out other steps. According to the invention, a plurality of microsatellite sites are selected, are distributed in a plurality of autosomes, and are low in linkage, large in the number of alleles, and high in polymorphism and heterozygosity; the landing PCR amplification is utilized, so that the operation is convenient, the product specificity is strong, andthe Hu sheep family can be quickly and accurately identified and divided; and the accumulated single parent exclusion rate, the paternity exclusion rate and the amphiphilic exclusion rate reach 99.6072%, 99.8808% and 99.9999% respectively, the requirements of paternity test and pedigree analysis in genetic breeding can be completely met, and the method has great application value in Hu sheep family breeding and breeding.

The invention discloses a double umbilical snail SNP molecular marker and its application in traceability. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO.1, which is 358 bp in total, and there is a base mutation 220C→220A at the 220th base, which is specifically recognized by the Fnu4HI restriction endonuclease. In 93 experimental populations of umbilical snails from 3 different locations, it was found that the allele frequency of the SNP molecular marker in different locations was close, the polymorphism was rich, the difference in allele frequency distribution was small, and the heterozygosity was greater than 0.3, it is preliminarily determined that the SNP molecular marker can be used for DNA traceability of the double umbilical snail.

The invention discloses a new short nucleotide tandem repeat site and application thereof. A short tandem repeat locus G11S0001 has a sequence shown as SEQ ID NO.1, and can be used for preparation of (a) reagents or kits for genetic relationship analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity testing or blood relationship analysis; (d) reagents or kits for detecting whether maternal blood contamination exists in extracted amniotic fluid; and / or (e) kits for detecting whether leucocytes in a bone marrow transplantation receptor are substituted by donor cells. The short tandem repeat locus G11S0001 has high discrimination, and can effectively analyze the genetic relationship.

The invention discloses a breeding method of common mudflat shellfish by convergent cross. The method comprises the steps: mudflat shellfish parents from more than four different geographic populations are collected; the shellfish parents of different geographic populations are carried out eco-maturing; then dual reciprocal cross is carried between two geographic populations; a first filial generation of dual reciprocal cross combination is bred up by a three-stage method; then the convergent cross of the first filial generation of dual reciprocal cross is carried out so as to obtain a first filial generation of convergent cross; and the above steps are repeated on the basis thereof till the filial generation of convergent cross accumulates the good genes of all the geographic populations. The method overcomes the geographic isolation and the biological differences of sexual maturity and breeding among different geographic populations. Compared with the existing mudflat shellfish varieties, the shellfish integrates the good genes from more than four different geographic populations; the heterozygosity is improved greatly, thus causing the converged half-bred individual to presentcharacteristics of fast growing and high stress resistance.

The present invention discloses a new short nucleotide tandem repeat locus and its application, the short tandem repeat locus G4S0001, the sequence is shown in SEQ ID NO.1, used to prepare (a) for genetic relationship analysis (b) Reagents or kits for individual identification; (c) Reagents or kits for paternity testing or blood relationship analysis; (d) Used to detect whether there is maternal blood contamination in the extracted amniotic fluid and / or (e) a kit for detecting whether leukocytes in a recipient are replaced by donor cells after bone marrow transplantation. The short tandem repeat locus G4S0001 has a high degree of discrimination and can effectively analyze genetic relationships.

The invention discloses an improved crossing method of a common mudflat shellfish, comprising the steps: secondary instar parents of the mudflat shellfish from different geographic populations are collected; the sexual gland is matured simultaneously by eco-maturing; simple improved crossing between two geographic populations is carried out; a first filial generation of simple improved crossing is bred up by a three-stage method; the first filial generation of cross-breeding is carried out the improved crossing with an introduced population; the first filial generation of cross-breeding is carried out the progressive improved crossing with a native population so as to obtain a filial generation of improved crossing; on the basis of that, the steps are repeated, the filial generation of improved crossing is carried out back crossing with the parent population so as to lead the filial generation of cross-breeding to maintain the heterosis thereof. The operation of the method is simple and convenient, and has high practicability, effectively enhances the resistance capability of the mudflat shellfish against the drastic changes of environmental factors, namely, temperature, salinity and the like, causes the improvement of the growth and survivability thereof and leads the maintenance of heterosis, and lays the foundation for the development of the fine variety of mudflat shellfish with high yield and stress resistance.

The invention provides a method for identifying the purity of Jingnongke 728 corn hybrid based on SNP markers. Based on 384 SNP sites, the invention adopts a high-throughput KASP technology platform, and uses more than 500 samples to carry out test effects, biological The comprehensive evaluation of characteristics and polymorphism parameters finally determined 4 SNP markers with high quality, high stability, high polymorphism and high heterozygosity, and designed 3 primers to amplify each SNP marker, a total of 12 primers. The nucleotide sequences are shown in SEQ ID NO.1-12, respectively. The four pairs of primer combinations can be used to identify the seed purity of Jingnongke 728, and can identify self-crossed seedlings, backcrossed seedlings and heterotypic strains. The invention expands the type and method of identification markers for the purity of corn hybrids, which is the popularization of Jingnongke 728. Planting provides a strong technical guarantee.

The present invention discloses a new short nucleotide tandem repeat locus and its application, the short tandem repeat locus G5S0003, the sequence is shown in SEQ ID NO: 1, used to prepare (a) for genetic relationship analysis (b) Reagents or kits for individual identification; (c) Reagents or kits for paternity testing or blood relationship analysis; (d) Used to detect whether there is maternal blood contamination in the extracted amniotic fluid and / or (e) a kit for detecting whether leukocytes in a recipient are replaced by donor cells after bone marrow transplantation. The short tandem repeat locus G5S0003 has a high degree of discrimination and can effectively analyze genetic relationships.

The invention provides a set of primers for identifying the purity of corn varieties and its application. The primer set of the present invention consists of primer pair 1, primer pair 2, primer pair 3, primer pair 4, primer pair 5, primer pair 6, primer pair 7 and primer pair 8. It is proved by experiments that the complete set of primers of the present invention has the characteristics of high heterozygosity, high variety recognition rate, high detection efficiency, low detection cost and the like, and can quickly and accurately obtain the identification result of seed purity. In the purity identification of corn single-cross varieties, there is no need to do the screening of variety purity identification primers and small sample experiments, and the set of primers is no longer limited to the identification of the purity of individual varieties, and can be applied to most varieties in China Rapid identification of purity.

The invention provides a method for identifying the purity of Jingke 968 corn hybrid based on SNP markers. Based on 384 SNP sites, the invention adopts a high-throughput KASP technology platform, and uses more than 500 samples to conduct test effects and biological characteristics. As well as comprehensive evaluation of polymorphism parameters, 4 SNP markers with high quality, high stability, high polymorphism and high heterozygosity are finally determined, and 3 primers for each SNP marker are designed to amplify a total of 12 primers. The nucleotide sequences are respectively as SEQ ID NO.1-12. The four pairs of primer combinations can be used to identify the seed purity of Jingke 968, and can identify self-crossed seedlings, backcrossed seedlings and heterotypic strains. The invention expands the type and method of identification markers for the purity of corn hybrids, and provides the promotion and planting of Jingke 968. strong technical support.

The present invention discloses a new short nucleotide tandem repeat locus and its application, the short tandem repeat locus G5S0001, the sequence is shown in SEQ ID NO.: 1, used to prepare (a) for genetic relationship Reagents or kits for analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity testing or blood relationship analysis; (d) for detecting whether there is maternal blood in the extracted amniotic fluid Contaminated reagents or kits; and / or (e) kits for detecting whether leukocytes in recipients have been replaced by donor cells after bone marrow transplantation. The short tandem repeat locus G5S0001 has a high degree of discrimination and can effectively analyze genetic relationships.

The invention relates to a paddy rice seed production method and an application thereof. The production method comprises the following steps: S1: rice variety A and rice variety B are subjected to hybridization or multi-variety composite hybridization, or biological, physical, and chemical methods to generate a property heterozygote, and F1-generation paddy rice hybrid seeds are collected; S2: theF1-generation paddy rice hybrid seeds are sown and cultured, and the F2-generation paddy rice seeds are collected; S3: the F2-generation paddy rice seeds are sown and cultured, and the F3-generationpaddy rice seeds are collected; S4: the F3-generation paddy rice seeds are sown and cultured, and then are subjected to self-hybridization, and the F4-generation paddy rice seeds are collected; and S5-1: the F4-generation paddy rice seeds are sown and cultured, colony stability identification is carried out, the F3-generation rice stubble corresponding to the stable and standard F4-generation colony is subjected to regeneration breeding; a material after regeneration breeding is continuously cultivated, and after self-hybridization, the seeds are collected to obtain the required seeds.