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38results about How to "High heterozygosity" patented technology

Hi-C high-throughput sequencing and database building method for eukaryote DNA

ActiveCN105839196ACyclization efficiency is lowLow effective data rateMicrobiological testing/measurementLibrary creationAdditive ingredientPolyethylene glycol
The invention relates to a Hi-C high-throughput sequencing and database building method for eukaryote DNA. According to the DNA-cell-nucleuse intramolecular cyclization step, end-repaired DNA and a cyclizing system are mixed, incubated for 2 h-4 h at the temperature of 4 DEG C to 6 DEG C and then incubated for 4 h-6 h at the temperature of 16 DEG C to 22 DEG C, and materials obtained after intramolecular cyclization are obtained, wherein the ingredients of the cyclizing system comprise T4 ligase damping liquid, BSA, T4 DNA ligase and at least one polyethylene glycol selected from PEG 4000 and PEG 6000. By means of the Hi-C high-throughput sequencing and database building method, the character cyclizing efficiency of DNA can be effectively improved, and the effective data rate obviously higher than that of a traditional method is obtained.
Owner:BIOMARKER TECH

Common intertidal shellfish polymerization hybridization breeding method

The invention discloses a breeding method of common mudflat shellfish by convergent cross. The method comprises the steps: mudflat shellfish parents from more than four different geographic populations are collected; the shellfish parents of different geographic populations are carried out eco-maturing; then dual reciprocal cross is carried between two geographic populations; a first filial generation of dual reciprocal cross combination is bred up by a three-stage method; then the convergent cross of the first filial generation of dual reciprocal cross is carried out so as to obtain a first filial generation of convergent cross; and the above steps are repeated on the basis thereof till the filial generation of convergent cross accumulates the good genes of all the geographic populations. The method overcomes the geographic isolation and the biological differences of sexual maturity and breeding among different geographic populations. Compared with the existing mudflat shellfish varieties, the shellfish integrates the good genes from more than four different geographic populations; the heterozygosity is improved greatly, thus causing the converged half-bred individual to present characteristics of fast growing and high stress resistance.
Owner:DALIAN FISHERIES UNIVERSITY

Reagent kit for fast detecting common chromosome trisome by quantitative fluorescence PCRs

The invention relates to a method for fast detecting common chromosome trisome by quantitative fluorescence PCR, which comprises the following steps: sample collection and processing, DNA extraction, primer design and synthesis, PCR amplified reaction of a first system and a second system, PCR product detection, PCR amplified reaction of a third system and PCR product detection. The invention also provides an accurate and reliable reagent kit for fast detecting the common chromosome trisome by the quantitative fluorescence PCR. The invention is integrated with the sensitivity and the accuracy of the quantitative fluorescence PCR and the extensive and uniform distribution, the polymorphism and the high heterozygosity of a tandem repeat sequence and can be used for detecting the number of common chromosomal disorders of prenatal samples, such as peripheral blood, amniotic fluids, cord blood, tomenta, and the like.
Owner:广州医学院

Method for identifying purity of Jingnongke 728 corn hybrids based on SNP markers

The present invention provides a method for identifying purity of Jingnongke 728 corn hybrids based on SNP markers. Based on 384 SNP sites, a high-throughput KASP technology platform is used, 500 or more samples are used for comprehensive evaluation of experimental effects, biological characteristics and polymorphic parameters, 4 of the SNP markers with high quality, high stability, high polymorphism and high heterozygosity are finally determined, 3 primers for each SNP marker and a total of 12 primers are designed and amplified. Nucleotide sequences are shown in SEQ ID NO.1-12, respectively.The 4 pairs of the primer combinations can be used to identify seed purity of Jingnongke 728 and can identify selfing, backcrossing, and heteromorphic plants. Types and methods of the purity identification markers for the corn hybrids are expanded to provide a strong technical guarantee for promoted planting of the Jingnongke 728.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Method for identifying purity of Jingke 968 corn hybrids based on SNP markers

The invention provides a method for identifying the purity of Jingke 968 corn hybrids based on SNP markers. The method is based on 384 SNP loci, a high-flux KASP technology platform is adopted, more than 500 samples are subjected to comprehensive evaluation of test effects, biological characteristics and polymorphic parameters, finally, four SNP markers with high quality, high stability, high polymorphism and high heterozygosis rate are determined, three primers of each SNP marker are designed and amplified, and the 12 primers have the nucleotide sequences shown as SEQ ID NO.1-12 respectively.By utilizing the four pairs of primer combination, the seed purity of Jingke 968 can be identified, selfing seedlings, backcross seedlings and special-shaped plants can be identified, the types and methods of corn hybrid purity identification markers are expanded, and a powerful technical guarantee is provided for popularization and planting of Jingke 968.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Gene variation site for regulating and controlling carcass indexes of Guizhou Xiang pigs and application of gene variation site

The invention discloses a gene variation site for regulating and controlling carcass indexes of Guizhou Xiang pigs and application of the gene variation site, belongs to the field of molecular biology, provides 10 SNPs sites, and provides application of the SNPs sites in regulating and controlling animal carcass performance and pig fat deposition. Correlation analysis is carried out on the detected 10 SNPs sites and 5 carcass meat quality characters of Guizhou Congjiang Xiang pigs, and the correlation between the 10 SNPs sites and test group characters is defined; according to the invention, amolecular basis can be provided for breeding pig carcass traits in the future, and a foundation is laid for genetic improvement of pig fat traits by using the gene in the future.
Owner:GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY

Nucleic acid mass spectrometry paternity testing method based on information SNP (Single Nucleotide Polymorphism) set and primers of information SNP set

The invention discloses a nucleic acid mass spectrometry paternity testing method based on an information SNP (Single Nucleotide Polymorphism) set and primers of information SNP set. The information SNP set used in the method which is a data analysis based on forty groups of people has a higher heterozygous rate greater than 0.4, a population genetic index Fst less than 0.06, lower mutation frequency less than 0.01 percent and a random population matching rate less than 10<-15>, and is suitable for individual identification and paternity identification, wherein the nucleotide sequences of thirty information SNP-marked sense primers are sequentially shown as SEQ ID NO.1-30, the nucleotide sequences of antisense primers are sequentially shown as SEQ ID NO.31-60, and the nucleotide sequencesof single-base extension primers are sequentially shown as SEQ ID NO.61-90. Verified by a flight mass spectrometry information SNP polymorphism and paternity inference experiment, a testing result matches an actual genetic relationship. When the information SNP set provided by the invention is used for human parentage testing, results are accurate, the mass spectrometry method is preferred to be utilized, genetic typing can be quickly and conveniently carried out at high throughput, and thereby a parentage index is calculated and paternity judgment is carried out. The method has the advantagesof high accuracy, low cost and the like.
Owner:PRIMBIO GENES BIOTECH WUHAN CO LTD

Paddy rice seeds production method and application thereof

The invention relates to a paddy rice seed production method and an application thereof. The production method comprises the following steps: S1: rice variety A and rice variety B are subjected to hybridization or multi-variety composite hybridization, or biological, physical, and chemical methods to generate a property heterozygote, and F1-generation paddy rice hybrid seeds are collected; S2: theF1-generation paddy rice hybrid seeds are sown and cultured, and the F2-generation paddy rice seeds are collected; S3: the F2-generation paddy rice seeds are sown and cultured, and the F3-generationpaddy rice seeds are collected; S4: the F3-generation paddy rice seeds are sown and cultured, and then are subjected to self-hybridization, and the F4-generation paddy rice seeds are collected; and S5-1: the F4-generation paddy rice seeds are sown and cultured, colony stability identification is carried out, the F3-generation rice stubble corresponding to the stable and standard F4-generation colony is subjected to regeneration breeding; a material after regeneration breeding is continuously cultivated, and after self-hybridization, the seeds are collected to obtain the required seeds.
Owner:雅安雨禾农业科技有限责任公司

New short nucleotide tandem repeat sequence locus and application thereof

The invention discloses a new short nucleotide tandem repeat sequence locus and application thereof. The invention provides the short tandem repeat locus G2S0002, the sequence of which is shown as SEQ ID NO.:1, and the short tandem repeat locus G2S0002 can be used for preparation of: (a) reagents or kits for genetic relationship analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity test or blood relationship analysis; (d) reagents or kits for detecting whether maternal blood contamination exists in extracted amniotic fluid; and / or (e) kits for detecting whether white blood cells in a bone marrow transplantation receptor is replaced by donor cells. The short tandem repeat locus G2S0002 has a high degree of distinction, and can effectively analyze the genetic relationship.
Owner:GENESKY DIAGNOSTICS SUZHOU

InDel marker suitable for corn genotyping and application of InDel marker

The invention provides an InDel marker suitable for corn genotyping and an application of the InDel marker. The InDel marker has information as shown in a table 1. The 1011 InDel markers and the combination thereof can be applied to corn variety DNA fingerprint database construction, corn variety authenticity identification, corn variety purity detection, corn germplasm resource clustering analysis, corn new breeding material genetic background analysis and screening, corn molecular marker assisted breeding and corn agronomic trait related gene genetic analysis, and wide application prospectsare realized.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

A hi-c high-throughput sequencing library construction method for eukaryotic DNA

ActiveCN105839196BImprove feature cyclization efficiencyIncrease effective data rateMicrobiological testing/measurementLibrary creationPolyethylene glycolOrganism
The invention relates to a Hi-C high-throughput sequencing and database building method for eukaryote DNA. According to the DNA-cell-nucleuse intramolecular cyclization step, end-repaired DNA and a cyclizing system are mixed, incubated for 2 h-4 h at the temperature of 4 DEG C to 6 DEG C and then incubated for 4 h-6 h at the temperature of 16 DEG C to 22 DEG C, and materials obtained after intramolecular cyclization are obtained, wherein the ingredients of the cyclizing system comprise T4 ligase damping liquid, BSA, T4 DNA ligase and at least one polyethylene glycol selected from PEG 4000 and PEG 6000. By means of the Hi-C high-throughput sequencing and database building method, the character cyclizing efficiency of DNA can be effectively improved, and the effective data rate obviously higher than that of a traditional method is obtained.
Owner:BIOMARKER TECH

Novel short nucleotide tandem repeat sequence sites and use thereof

The invention discloses a novel short nucleotide tandem repeat sequence sites and a use thereof. The sequence of the short tandem repeat sequence gene-locus G8S0001 is shown in the formula of SEQ ID NO: 1. The short tandem repeat sequence gene-locus G8S0001 is used for preparation of (a) a reagent or a kit for genetic relationship analysis, (b) a reagent or a kit for individual identification, (c) a reagent or a kit for paternity testing or blood relationship analysis, (d) a reagent or a kit for detecting if amniotic fluid extract is polluted by mother blood, and / or (e) a kit for detecting if leucocytes in an acceptor subjected to bone marrow transplantation are replaced by donor cells. The short tandem repeat sequence gene-locus G8S0001 has high discrimination and can realize effective analysis of a genetic relationship.
Owner:GENESKY DIAGNOSTICS SUZHOU

Common intertidal shellfish polymerization hybridization breeding method

The invention discloses a breeding method of common mudflat shellfish by convergent cross. The method comprises the steps: mudflat shellfish parents from more than four different geographic populations are collected; the shellfish parents of different geographic populations are carried out eco-maturing; then dual reciprocal cross is carried between two geographic populations; a first filial generation of dual reciprocal cross combination is bred up by a three-stage method; then the convergent cross of the first filial generation of dual reciprocal cross is carried out so as to obtain a first filial generation of convergent cross; and the above steps are repeated on the basis thereof till the filial generation of convergent cross accumulates the good genes of all the geographic populations. The method overcomes the geographic isolation and the biological differences of sexual maturity and breeding among different geographic populations. Compared with the existing mudflat shellfish varieties, the shellfish integrates the good genes from more than four different geographic populations; the heterozygosity is improved greatly, thus causing the converged half-bred individual to presentcharacteristics of fast growing and high stress resistance.
Owner:DALIAN FISHERIES UNIVERSITY

New short nucleotide tandem repeat site and application thereof

The invention discloses a new short nucleotide tandem repeat site and application thereof. A short tandem repeat locus G11S0001 has a sequence shown as SEQ ID NO.1, and can be used for preparation of (a) reagents or kits for genetic relationship analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity testing or blood relationship analysis; (d) reagents or kits for detecting whether maternal blood contamination exists in extracted amniotic fluid; and / or (e) kits for detecting whether leucocytes in a bone marrow transplantation receptor are substituted by donor cells. The short tandem repeat locus G11S0001 has high discrimination, and can effectively analyze the genetic relationship.
Owner:GENESKY DIAGNOSTICS SUZHOU

New Short Nucleotide Tandem Repeat Sequence Site and Its Application

The present invention discloses a new short nucleotide tandem repeat locus and its application, the short tandem repeat locus G4S0001, the sequence is shown in SEQ ID NO.1, used to prepare (a) for genetic relationship analysis (b) Reagents or kits for individual identification; (c) Reagents or kits for paternity testing or blood relationship analysis; (d) Used to detect whether there is maternal blood contamination in the extracted amniotic fluid and / or (e) a kit for detecting whether leukocytes in a recipient are replaced by donor cells after bone marrow transplantation. The short tandem repeat locus G4S0001 has a high degree of discrimination and can effectively analyze genetic relationships.
Owner:GENESKY DIAGNOSTICS SUZHOU

Common intertidal shellfish hybridization method

The invention discloses an improved crossing method of a common mudflat shellfish, comprising the steps: secondary instar parents of the mudflat shellfish from different geographic populations are collected; the sexual gland is matured simultaneously by eco-maturing; simple improved crossing between two geographic populations is carried out; a first filial generation of simple improved crossing is bred up by a three-stage method; the first filial generation of cross-breeding is carried out the improved crossing with an introduced population; the first filial generation of cross-breeding is carried out the progressive improved crossing with a native population so as to obtain a filial generation of improved crossing; on the basis of that, the steps are repeated, the filial generation of improved crossing is carried out back crossing with the parent population so as to lead the filial generation of cross-breeding to maintain the heterosis thereof. The operation of the method is simple and convenient, and has high practicability, effectively enhances the resistance capability of the mudflat shellfish against the drastic changes of environmental factors, namely, temperature, salinity and the like, causes the improvement of the growth and survivability thereof and leads the maintenance of heterosis, and lays the foundation for the development of the fine variety of mudflat shellfish with high yield and stress resistance.
Owner:DALIAN FISHERIES UNIVERSITY

Novel short nucleotide tandem repeat sequence sites and use thereof

The invention discloses novel short nucleotide tandem repeat sequence sites and a use thereof. The sequence of the short tandem repeat sequence gene-locus G5S0003 is shown in the formula of SEQ ID NO: 1. The short tandem repeated sequence gene-locus G5S0003 is used for preparation of (a) a reagent or a kit for genetic relationship analysis, (b) a reagent or a kit for individual identification, (c) a reagent or a kit for paternity testing or blood relationship analysis, (d) a reagent or a kit for detecting if amniotic fluid extract is polluted by mother blood, and / or (e) a kit for detecting if leucocytes in an acceptor subjected to bone marrow transplantation are replaced by donor cells. The short tandem repeat sequence gene-locus G5S0003 has high discrimination and can realize effective analysis of a genetic relationship.
Owner:GENESKY DIAGNOSTICS SUZHOU

New short nucleotide tandem repeat site and application thereof

The invention discloses a new short nucleotide tandem repeat site and application thereof. A short tandem repeat locus G7S0005 has a sequence shown as SEQ ID NO.1, and can be used for preparation of (a) reagents or kits for genetic relationship analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity testing or blood relationship analysis; (d) reagents or kits for detecting whether maternal blood contamination exists in extracted amniotic fluid; and / or (e) kits for detecting whether leucocytes in a bone marrow transplantation receptor are substituted by donor cells. The short tandem repeat locus G7S0005 has high discrimination, and can effectively analyze the genetic relationship.
Owner:GENESKY DIAGNOSTICS SUZHOU

New short nucleotide tandem repeat site and application thereof

The invention discloses a new short nucleotide tandem repeat site and application thereof. A short tandem repeat locus G5S0001 has a sequence shown as SEQ ID NO.1, and can be used for preparation of (a) reagents or kits for genetic relationship analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity testing or blood relationship analysis; (d) reagents or kits for detecting whether maternal blood contamination exists in extracted amniotic fluid; and / or (e) kits for detecting whether leucocytes in a bone marrow transplantation receptor are substituted by donor cells. The short tandem repeat locus G5S0001 has high discrimination, and can effectively analyze the genetic relationship.
Owner:GENESKY DIAGNOSTICS SUZHOU

A Nucleic Acid Mass Spectrometry Parentage Testing Method Based on Informational SNP Set and Its Primers

The invention discloses a nucleic acid mass spectrometry paternity identification method based on an information SNP set and its primers. The information SNP set used in the method is based on the data analysis of 40 populations, has a high heterozygosity rate>0.4, and has a population genetic Index Fst<0.06, and low mutation frequency <0.01%, random population matching rate <10 ‑15 , suitable for individual identification and paternity identification, wherein the nucleotide sequences of the upstream primers of the 30 informational SNP markers are shown in SEQ ID NO.1~30, and the nucleotide sequences of the downstream primers are shown in SEQ ID NO.31~ As shown in 60, the nucleotide sequence of the single base extension primer is shown in sequence as SEQ ID NO.61-90. After flight mass spectrometry detection information SNP polymorphism and paternity inference test verification, the identification result is consistent with the actual kinship relationship; the information SNP set provided by the invention is used for human paternity identification, and the result is accurate. The mass spectrometry method is preferably used, which can be fast and high-throughput , Simple genotyping, so as to calculate parentage index and carry out parentage judgment, which has the advantages of high accuracy and low cost.
Owner:PRIMBIO GENES BIOTECH WUHAN CO LTD

New Short Nucleotide Tandem Repeat Sequence Site and Its Application

The present invention discloses a new short nucleotide tandem repeat locus and its application, the short tandem repeat locus G5S0003, the sequence is shown in SEQ ID NO: 1, used to prepare (a) for genetic relationship analysis (b) Reagents or kits for individual identification; (c) Reagents or kits for paternity testing or blood relationship analysis; (d) Used to detect whether there is maternal blood contamination in the extracted amniotic fluid and / or (e) a kit for detecting whether leukocytes in a recipient are replaced by donor cells after bone marrow transplantation. The short tandem repeat locus G5S0003 has a high degree of discrimination and can effectively analyze genetic relationships.
Owner:GENESKY DIAGNOSTICS SUZHOU
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