39results about How to "Characteristics unchanged" patented technology

The invention relates to an improved mesenchyme stem cell protection solution as well as application and a preparation method thereof. The protection solution can effectively prolong the activity remaining time of mesenchyme stem cells, reduces preparation cost, and has the advantages of wide raw material source, simple preparation, safe and reliable direct clinical application; and after the mesenchyme stem cells are preserved for 48 hours in the protection solution, the cell activity is still above 90 percent, the cell morphology is normal, and the multiplication capacity and mesenchyme stem cell phenotype characteristics are not influenced.

The invention discloses a tissue culture and rapid propagation method for sspindus mukurossi. The sspindus mukurossi is also named as soapbark, is a deciduous tree, is mainly propagated by seed sowing, and is hard in seed coat, high in moth rate, irregular in seedling emergence and long in growth period. Defects of seed propagation can be made up by tissue culture, the excellent characteristics of a mother plant are kept unchanged, the propagation speed is high, the propagation coefficient is high, the production cost can be reduced, and the needs of large-scale planting of the sspindus mukurossi are met. A stem with nodes of the sspindus mukurossi is taken as an explant, a sspindus mukurossi in-vitro replanting plant is successfully obtained by adventive bud induction culture, multiplication culture, rooting culture, acclimatization and transplantation, and the like, a sspindus mukurossi tissue culture and rapid propagation system is established, an effective way is provided for the large-scale seedling growing of the sspindus mukurossi, and foundation is laid for the factorial production and application of sspindus mukurossi nursery-grown plants.

The invention discloses a human thymoma cell line and application thereof, belonging to the field of tumor biology. The human thymoma cell line is named Thy0517 and has a collection number of CGMCC (China General Microbiological Culture Collection Center) No.9328. The human thymoma cell line Thy0517 has main advantages of having protein expression similar to primary tumor tissues, abnormal karyotype and characteristics of tumor cells with in-vitro tumor forming capability. After in-vitro continuous passage culture, the characteristics of thymoma cells keep unchanged, and therefore the human thymoma cell line can be used as a cell material for researching and developing thymoma-related drugs. EGFR (Epidermal Growth Factor Receptor) expression of the human thymoma cell line is positive, so that proliferation, angiogenesis, adhesion, invasion and metastasis of the tumor cells can be promoted; the cell line can be used as a powerful cell tool for researching EGFR-targeted drugs.

The present invention discloses coating apparatus and process for long fiber grating. The coating apparatus consists of high precision mobile platform, stress adjustable optical regulator, controllable coating material container, coating pliers, ultrasonic light source, reflector, etc. and has relatively low cost. The coating process includes shifting the high precision mobile platform, regulating the optical regulator, regulating the stress, controlled coating the fiber grating, and curing the coated layer with the precisely controlled ultrasonic light source. The present invention has adjustable coating length up to 200 mm, unchanged characteristic of the coated fiber grating and raised mechanical stress performance.

The invention relates to an array imaging system. The system comprises a base and a silicon lens array which is arranged on the base and formed by a plurality of silicon lenses, an anti-reflect layer with thickness of a quarter of a wavelength is plated on the surface of the frontal side of each of the silicon lens, a terahertz probe is installed in the center of the back of each of the silicon lens to form a terahertz probe array, and a terahertz wave-absorbing material layer is covered on a metal surface among the plurality of the silicon lenses on the frontal side of the base.

The invention discloses a method for quickly constructing a low-dimensional reduced-basis space in electromagnetic scattering modeling. The method comprises the steps of firstly, selecting out a domain or a boundary which determines a final output variable from geometric domains or geometric boundaries of a to-be-simulated structure, wherein the selected domain or boundary is called as a main domain or a main boundary; secondly, based on an original high-dimensional model, constructing a high-dimensional partial model about a variable in the main domain or on the main boundary, and constructing a reduced-basis space for the high-dimensional partial model in a parameterization environment, wherein the obtained reduced-basis space is called as a partial reduced-basis space; and finally, projecting the high-dimensional partial model to the partial reduced-basis space to obtain an approximate low-dimensional model, and replacing solving of the original high-dimensional model with solving of the low-dimensional model, thereby improving the solving efficiency of a simulation model is improved. The method is especially suitable for the situation that the final output variable is determined only by a partial solution in the high-dimensional model. Compared with a conventional reduced-basis method, the method has the advantages that the dimensions of the reduced-basis space are further reduced (correspondingly, the dimensions of the low-dimensional model are further reduced) and the construction speed of the reduced-basis space is greatly increased.

The invention discloses a method for culturing and cryopreservation of CIK cells by applying a serum-free lymphocyte culture medium and obtained CIK cells. The cell culture method can develop 3*10<9> lymphocytes in a short time without introducing animal derived serum; the cell cryopreservation method can maximally improve the ratio of cryopreserved target cells and the survival rate after cell recovery. The method provides convenience for culturing, storing and related clinical research of the CIK cells.

The invention relates to the field of a cell biotechnology, relates to a cell cryopreservation method and particularly relates to an improved rapid freezing method. The cell cryopreservation method can be used for improving the activity of cells under the condition of ensuring that the cell characteristics are unchanged. The cell cryopreservation method has the characteristics that a gauze bag with a hole is adopted, a freezing tube is suspended in a liquid nitrogen steam layer so as to ensure that the cells in the freezing tube are rapidly cooled, and the activity of the resuscitated cells reaches up to 95%-99%; 10% of DMSO (dimethylsulfoxide) and 90% of serum are used as cryopreservation liquid, so that the permeability of a cell membrane to water can be improved, crystals outside the cells are frozen within short time, and the permeation of moisture is prevented from causing the damage to the cells. The cell cryopreservation method is suitable for the long-term storage of all cell lines. Compared with the prior art, the cell cryopreservation method has the advantages that steps are omitted, the technology is simplified, the resuscitated cells have high activity and unchanged cell characteristics, and the long-term storage purpose can be realized.

The invention discloses an organic fertilizer produced by cornflower herbs and a production method of the organic fertilizer. The organic fertilizer comprises, by weight, 70-90 parts of cornflower herbs, 3-7 parts of pine needles, 3-7 parts of eucalyptus leaves, 1-3 parts of moxa sticks, 1-3 parts of industrial hydrochloric acid, 1-3 parts of absolute ethyl alcohol, 0.8-1.2 parts of oil cakes, 0.01-0.03 part of industrial paclitaxel and 1-3 parts of quicklime. The cornflower herbs can be produced into the organic fertilizer with good effects by the method, and the organic fertilizer can be popularized and used in vast rural areas and has good economic benefits.

The invention relates to the field of protein purification and particularly relates to a tissue treatment device for protein purification. The technical problem to be solved by the invention is to provide the tissue treatment device for protein purification. The tissue treatment device for protein purification comprises a machine body bottom plate, an isolation sampling mechanism, an internal tissue sampling mechanism, a tissue cell breaking mechanism, an external sealing communication mechanism, an operation control screen and the like, wherein the top left side of the body bottom plate is welded to the top deck. The tissue treatment device is advantaged in that the sterile sample input is realized, sealing, isolating and sampling after disinfection of the sample are completed, the deviceextends into original sample tissues to extract internal fresh tissues, cell characteristics are unchanged, protein is invariant, interference of original impurities and bacteria on an outer surfaceof a sample is prevented, fine grinding after mincing is achieved, the device is always in a sealed and isolated state before and after treatment, and sterile cell mixed liquid is obtained.