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A kind of human thymoma cell line and its application

A thymoma and cell line technology, applied in the field of human thymoma cell lines and their uses, can solve problems such as lack of functional detection of thymoma cell lines, and achieve the effect of promoting proliferation

Inactive Publication Date: 2016-06-08
张鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These preliminary studies have shown that some targeted drugs are effective in the treatment of thymoma, but due to the lack of functional assays in thymoma cell lines in vitro, many new targeted therapies can be used in other tumors, but have not yet been tested in thymoma.

Method used

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  • A kind of human thymoma cell line and its application
  • A kind of human thymoma cell line and its application
  • A kind of human thymoma cell line and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The establishment of embodiment 1 human thymoma cell lines

[0027] The human thymoma cell line Thy0517 of the present invention is a cell line of type AB thymoma from northern China, derived from the thymoma tissue of a 50-year-old male patient with thymoma combined with myasthenia gravis. The patients did not receive any chemotherapy, radiotherapy and other treatments before undergoing surgery. The human thymoma cell line Thy0517 is cultured from the thymoma tissue excision of a thymoma patient whose pathological type is type AB thymoma.

[0028] The specific steps are:

[0029] (1) Mechanical separation of tissue blocks: The surgically resected thymoma tissue was quickly separated to a size of 0.5 cubic centimeters with sterile scissors and a scalpel, and soaked in PBS buffer containing 100U / ml penicillin and 100ug / ml streptomycin Go to the biological safety cabinet, then repeatedly wash the tissue with PBS buffer containing 100U / ml penicillin and 100ug / ml streptom...

Embodiment 2

[0032] Example 2 Detection and Identification of Human Thymoma Cell Line Thy0517

[0033] 1. Morphological observation

[0034] Mainly observe the general morphology of cells, such as gross shape, nuclear-to-cytoplasmic ratio, adhesion characteristics, etc.

[0035] Observation of human thymoma cell line Thy0517 under an inverted optical phase-contrast microscope figure 1 As shown, it can be seen that the cells are polygonal, with large nuclei, abundant cytoplasm, tight connections between cells, and adherent growth.

[0036] 2. Immunocytochemical detection of cell marker proteins

[0037] Immunocytochemical staining was carried out by SP method. Immunocytochemical staining SP kit and antibodies were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. The antibodies used were: CK7, CK8 / 18, CK19, CK-pan, CD24, BCL-2 , P63, Vimentin, EMA, EGFR and CD99.

[0038] The specific steps were carried out according to the instructions of the kit: observe the staining re...

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Abstract

The invention discloses a human thymoma cell line and application thereof, belonging to the field of tumor biology. The human thymoma cell line is named Thy0517 and has a collection number of CGMCC (China General Microbiological Culture Collection Center) No.9328. The human thymoma cell line Thy0517 has main advantages of having protein expression similar to primary tumor tissues, abnormal karyotype and characteristics of tumor cells with in-vitro tumor forming capability. After in-vitro continuous passage culture, the characteristics of thymoma cells keep unchanged, and therefore the human thymoma cell line can be used as a cell material for researching and developing thymoma-related drugs. EGFR (Epidermal Growth Factor Receptor) expression of the human thymoma cell line is positive, so that proliferation, angiogenesis, adhesion, invasion and metastasis of the tumor cells can be promoted; the cell line can be used as a powerful cell tool for researching EGFR-targeted drugs.

Description

technical field [0001] The invention relates to a human thymoma cell line and its application, belonging to the field of tumor biology, in particular to a human thymoma cell line Thy0517 and its application. Background technique [0002] The thymus is the central organ of the immune system and the place where T cells develop, differentiate and mature. Its function is to differentiate some lymphocytes into T lymphocytes. The thymus, like other organs in the human body, can develop benign or malignant tumors. Thymomas are the most common tumors of the anterior mediastinum, accounting for 30% of anterior mediastinal tumors in adults, and are tumors that arise from the thymic epithelium or show differentiation toward thymic epithelial cells (regardless of the presence or absence of non-neoplastic lymphocytes). The incidence of thymoma in China is relatively high, and it is more likely to occur in the 55-56 age group. Although mortality in patients with thymoma is low, patients...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09C12Q1/02A01K67/027C12R1/91
Inventor 张鹏王元国
Owner 张鹏
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