The invention discloses an improved lung node puncture positioning needle assembly which comprises a puncture needle with an inner hole and a positioning guiding wire penetrating through the punctureneedle, a V-shaped barb is arranged at the positioning end of the positioning guiding wire, the end, opposite to the positioning end, of the positioning guiding wire is a push end, and a reinforcing part is arranged at the portion, close to the positioning end, of the positioning guiding wire. The assembly is characterized in that a bent part with the memorizing function is arranged at the portion, close to the positioning end, of the positioning guiding wire, the bent part deforms to be linear when the positioning guiding wire is inserted into the inner hole of the puncture needle, and the bent part recovers to be bent after the positioning guiding wire gets away from the inner hole of the puncture needle. When the V-shaped barb of the positioning end of the positioning guiding wire hooksthe focus of the disease, and after the puncture needle is pulled out, the V-shaped barb on the positioning guiding wire is matched with the bent part, the positioning guiding wire is stopped from moving forwards and backwards, and the probability that the positioning guiding wire is disengaged from the focus of the disease to be retracted into the chest wall or slide into the mediastinum is eradicated.