Establishing method for rabbit sinus node chronic injury model and special electrode for implementing establishing method

Abstract

The invention provides an establishing method for a rabbit sinus node chronic injury model. The method comprises the following steps of: after anaesthetizing a rabbit, fixing the back of the rabbit on a rabbit table, and recording body surface electrocardiogram; shaving the skin at a colpus area, longitudinally opening the chest along a third rib at the right side of sternum, separating epidermis and muscular tissues layer by layer, cutting off the third rib of the right side, and opening pericardium through mediastinum to expose auricula dextra; placing an electrode connected with an electrocardiogram V1 lead to a near sinus node area of the rabbit and slowly moving, when the electrocardiogram displays electrograph characteristics of the sinus node area, applying 20% formaldehyde solution to the electrode area, when the heart rate of the rabbit is decreased to 50-70% of original heart rate, intravenously injecting 2mg of atropine sulphate until the heart rate stops rising, and stopping applying formaldehyde solution; and cleaning the chest, closing the chest layer-by-layer, and feeding for later use. The invention also provides a sinus node targeted injection penetration electrode for implementing the method. Compared with the prior art, the establishing method is high in modeling success rate, stable in model and good in feasibility and repeatability..

Description

technical field [0001] The invention belongs to the field of animal models in the medical field, and in particular relates to a method for establishing a rabbit sinoatrial node chronic injury model. Background technique [0002] Sick sinus syndrome (Sick Sinus Syndrome, SSS) is a common clinical refractory arrhythmia disease, its pathogenesis is not yet clear, studies have shown that sinus node cell damage leads to pacing and conduction dysfunction its immediate cause. Establishing a stable and reproducible sinus node chronic damage (Chronic Sinus Node Dysfunction, CSND) model is a prerequisite for experimental research on SSS. [0003] The key to the success of the CSND model is to cause chronic damage to the sinus node on the premise of reducing the damage to the tissues around the sinus node and other tissues and organs as much as possible, so that the electrophysiological indicators of the heart tend to be stable for a period of time after the model is established. Con...

AI Technical Summary

Problems solved by technology

These methods have the disadvantages of difficulty in accurately locating the sinoatrial node area, large damage range, and low survival rate.

For example, cryoablation of the sinus node area and radiofrequency ablation rely on large-scale equipment and are difficult to operate, so they are not suitable for establishing CSND models in smaller animals such as rabbits; ligation of sinus node blood supply arteries can successfully establish the sinus node In the acute injury model, the main blood supply of the rabbit sinus node comes from the right coronary artery, but the area of ​​the rabbit sinus node area is small, and the exposure of the right coronary artery is not good. Using this method can easily cause damage to other tissues of the heart, which is difficult for the establishment of CSND models Larger; although the formaldehyde wet compress method can establish a CSND model, it needs to make a 3-5cm incision on the body surface and clamp off two ribs, which causes relatively large damage. Damage to other tissues of the heart can easily cause right atrial appendage hemorrhage and pleural rupture leading to pneumothorax, which is not conducive to the survival of model animals after surgery

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