81 results about "Antioxidative stress" patented technology

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the aspects of enhancing cell defense and repair and regulating metabolic homeostasis of a host. The strain is isolated from infant feces. Animal experiments prove that living cell thalli of the bifidobacterium longum subsp. Infantis Y46, which are obtained by culturing an MRS liquid culture medium added with L-cysteine hydrochloride (0.05%, v / v), can significantly enhance the oxidative stress resistance of an organism and reduce the level of active oxygen in the organism compared with a control bacterium bifidobacterium longum subsp. Infantis 15697. Transcriptomics analysis shows that the bifidobacterium longum subsp. Infantis Y46 can enhance self-protection of cells and improve physiological imbalance caused by external pressure by regulating related signal channels of an organism. The action mechanism is closely related to signaling pathways related to cell defense, metabolic homeostasis and barrier functions. The invention provides a new strain resource and an application scheme for developing functional food or medicinal preparations for enhancing the health defense function of a human body.

The invention discloses application of copper-amino acid nano enzyme in preparation of anti-inflammatory drugs, and relates to the technical field of biomedical new materials, amino acids comprise glycine, arginine, histidine, threonine, phenylalanine and cysteine; the ratio of the amino acid to the copper ions is 1: (0.5-5), and the synthesis temperature of the copper-cysteine nano enzyme is 25-125 DEG C. The copper-amino acid nano-enzyme library established by the invention has efficient hydroxyl free radical, superoxide free radical and hydrogen peroxide scavenging activity; wherein the copper-cysteine nano-enzyme shows the highest enzymatic activity, and shows low toxicity and good biocompatibility in both the cell level and the animal level; meanwhile, the copper-cysteine nano-enzyme also shows anti-inflammatory activity and anti-oxidative stress activity, can remarkably improve cell inflammation and body inflammation, relieves and treats dextran sodium sulfate induced mouse ulcerative colitis, and can be further applied to preparation of drugs for treating inflammatory bowel diseases.

The invention provides a preparation method of human collagen and an application of the human collagen in anti-aging. The amino acid sequence of the human collagen is as shown in SEQ ID NO: 1; based on a natural collagen structure, a fusion sequence structure is provided, and the proportion of individual amino acids is adjusted; the collagen can promote skin cell growth and hyaluronic acid secretion; skin aging caused by ultraviolet radiation is resisted, inflammatory factor expression is inhibited, and oxidative stress is resisted.

The application belongs to the field of microbial metabolic engineering, and discloses a Saccharomyces cerevisiae engineering strain with enhanced isopentenol tolerance and conversion capacity, and a construction method and application thereof. The Saccharomyces cerevisiae engineering strain is obtained by modifying an isopentenol utilization pathway-dependent Saccharomyces cerevisiae strain, and the energy metabolism of the Saccharomyces cerevisiae strain is enhanced to synthesize a large amount of ATP for the isopentenol utilization pathway; and the antioxidant stress resistance of the Saccharomyces cerevisiae strain is enhanced to relieve the oxidative damage caused by the isopentenol substrate. The Saccharomyces cerevisiae engineering strain is the strain with the highest efficiency in converting isopentenol into terpenes.

The invention belongs to the technical field of compound application, and particularly relates to coriaria lactone compounds, a preparation method thereof and application of the coriaria lactone compounds in oxidative stress resistance. The coriaria lactone compound can significantly inhibit oxidative stress damage of SH-SY5Y cells, and the cell survival rate of the coriaria lactone compound is increased to 79.6%. Meanwhile, the nematode anti-aging activity is achieved, generation of nematode lipofuscin is inhibited, and the highest inhibition rate is 45%. In an extreme oxidative stress experiment, the service life of the nematodes is prolonged, so that the coriaria lactone compound has an application prospect of treating and resisting aging diseases.

This invention relates to a vulnerary multifunctional composition, based on unripe coffee cherries (peel, mucilage, beans and silverskin), seed extract of Trigonella foenum-graecum, with one or more tissue-inducing molecules derived from the secondary metabolites of both plants. The unexpected and surprising ability of this new composition to solve the problems of chronic non-healing wounds addresses a long felt unmet need in the medical arts. Through a multifaceted approach, it combats microbial infections, reduces chronic inflammation, counters oxidative stress, enhances immune responses, promotes angiogenesis, regulates protease activity, stimulates collagen deposition, increases growth factor availability, facilitates cell migration, and prevents persistent infections. These novel integrated therapies represent a breakthrough in addressing macroscopic wound healing while targeting the underlying microscale mechanisms. This invention also relates to methods for preparing, using, and administering the multifunctional composition for timely healing in mammals with various skin lesions.

The application discloses an eggshell membrane peptide with antioxidant and improving exercise capacity of aging organism and application thereof, and belongs to the technical field of biology. The amino acid sequence of the eggshell membrane peptide is shown as SEQ ID NO. 1, and is specifically EQPWFPAG. A novel antioxidant peptide EQPWFPAG is identified from chicken eggshell membrane hydrolysate, and the eggshell membrane peptide can improve the exercise capacity and antioxidant stress capacity of an aging organism through verification. The eggshell membrane peptide provided by the application can be used for preparing an antioxidant, and can also be used for preparing functional food, health products and pharmaceutical compositions for improving the exercise function of an aging organism, and has great application value.

The invention discloses a natural functional component for preventing and improving cardiovascular diseases of pets. The natural functional component comprises an earthworm protein extract and a leech extract, the earthworm protein extract accounts for 20wt%-40wt% of the natural functional components; the ratio of the leech extract in the natural functional components is 10wt%-30wt%; the invention also comprises a feed containing the natural functional components for preventing and improving cardiovascular diseases of pets. The traditional Chinese medicine composition has the effects of resisting blood coagulation, thrombus, inflammation and oxidative stress, can prevent thrombosis, relieve inflammatory response, reduce free radical damage and protect the health of heart and cerebral vessels, and plays a role in preventing and improving cardiovascular diseases of pets; the pet feed additive can be added into feed to protect pet health.

The present application relates to the technical field of nanomaterial application, and provides a ruthenium-doped Prussian blue nanoscale enzyme and a preparation method thereof, wherein the ruthenium-doped Prussian blue nanoscale enzyme is obtained by reacting metal ruthenium with Prussian blue nanoparticles under normal temperature conditions. The present application also provides a nanoscale enzyme library containing the ruthenium-doped Prussian blue nanoscale enzyme and a construction method thereof. The present application also provides SNP-PB nanoscale enzyme obtained by loading sodium nitroprusside into the ruthenium-doped Prussian blue nanoscale enzyme. The ruthenium-doped Prussian blue nanoscale enzyme and the SNP-PB nanoscale enzyme both have good antibacterial and anti-inflammatory properties, and can better solve the problem of biofilm resistance after being endowed with the ability to release NO gas, thereby better exerting antioxidant stress and fighting periodontal bacterial infection, and providing a new treatment method for periodontal disease.

The present application relates to the technical field of molecular markers, and particularly relates to a SNP molecular marker related to the anti-oxidative stress capacity of Tibetan sheep and application thereof.A SNP molecular marker related to the anti-oxidative stress capacity of Tibetan sheep, the molecular marker is located at the 196714361th base on the 3rd chromosome of the international sheep genome Oar_v4.0 version, and the mutation base is A or C.Through detecting the base of the 196714361th nucleotide site on the 3rd chromosome of the Tibetan sheep, the anti-oxidative stress index T-AOC content of the Tibetan sheep individual can be judged, and the present application provides a new SNP molecular marker resource for the marker-assisted selection of the anti-oxidative stress trait of the Tibetan sheep for non-diagnostic purposes.

The present application relates to a kind of biomimetic reticular nanofiber composite coating and its preparation method and infectious bone defect repair application, belong to biomedical material field.The present application is aimed at solving the technical problem of the poor bone integration of implant in infectious bone defect.The present application provides a kind of biomimetic reticular nanofiber composite coating, which is formed by layer-by-layer self-assembly on the surface of implant by lysozyme amyloid fiber and polyphenols (such as tannin etc.) Form reticular structure, then prepared with bioactive metal ions coordination;The number of coating layers is 4-8 layers.Preparation method includes lysozyme fiberization, alternate deposition and ion loading steps.The composite coating of the present application has broad-spectrum antibacterial, antioxidant stress and promotes osteogenesis function;In animal model, bacterial load is reduced by 80%, bone volume fraction and trabecular bone parameters are significantly improved, and can realize anti-bacterial biofilm infection-antioxidant stress-angiogenesis-promote bone regeneration synergy.

This invention concerns a method of using mycotoxin binder in broiler chicken feed to reduce the immunotoxicity of aflatoxin B1 and ochratoxin A. This method is implemented by mixing mycotoxin binder into broiler chicken feed contaminated with aflatoxin B1 and ochratoxin A at a certain dose, then providing the feed to broiler chickens during the rearing period. The application of this method is able to reduce the toxic impact of mixed mycotoxins on the immune system of broiler chickens by improving the immune response and protection against oxidative stress. This method provides benefits in the form of improving the health and endurance of broiler chickens and reducing economic losses in the poultry industry due to decreased performance and health of livestock.

The invention belongs to the field of feed additives, and particularly relates to a nano feed additive for improving intestinal flora and a preparation method thereof.The preparation method comprises the following steps that (1) an electric arc furnace with pure copper as an anode and a graphite rod as a cathode is provided, absolute ethyl alcohol is injected into the furnace body in the vacuum state in the electric arc furnace, the arc starting current is controlled to be 40-60 A, and the temperature is controlled to be 20-30 DEG C; and purifying in an argon atmosphere for 12-48 hours to obtain the Cu-coated C nano-particles. And (2) carrying out oxidation reaction on the Cu-coated C nano-particles in an oxygen-containing atmosphere at the reaction temperature of 200-350 DEG C for 1-5 hours to obtain an oxidized product, namely the nano feed additive. The nano feed additive disclosed by the invention can be used for preventing and treating bacterial infection, enhancing the immune function and the anti-oxidative stress function of an organism, improving intestinal flora and prolonging the service life of animals, is an effective and safe functional feed additive, is simple in preparation method and low in production cost, can realize large-scale preparation, and has an important application prospect.

The present invention relates to an animal model for oxidative stress research and use thereof, and more specifically, the present invention can utilize a mutant of RCAT having a regulatory function for an antioxidant stress regulator in Caenorhabditis elegans and a human cell line expressing RCAT as animal and human cell line models for oxidative stress research, using the mutant and the human cell line.

The present application belongs to the technical field of tissue transportation, and particularly relates to a tissue preservation and transportation device and a tissue preservation reagent, which aims to solve the technical problem that the activity of living tissue is reduced or the living tissue is dead after transportation in the prior art, and it is difficult to retain the original tissue characteristics. The tissue preservation and transportation device comprises a tissue preservation reagent and a preservation tube. The tissue preservation reagent comprises a temperature-sensitive gel, an antioxidant stress component and a tissue preservation solution. The preservation tube is used for containing the tissue preservation reagent. The tissue preservation and transportation device and the tissue preservation reagent utilize the tissue tube and the tissue preservation reagent to effectively buffer the jolt suffered by the tissue during transportation. The characteristics of the temperature-sensitive gel facilitate the transfer of the tissue block and the removal of the tissue block. The antioxidant stress component can keep the tissue characteristics unchanged. The tissue preservation solution can keep the tissue cell activity, and plays a good protection role on the tissue during transportation. The tissue preservation and transportation device provides a simple and effective method for tissue preservation and transportation.

The present application relates to the application of active ingredients in Lonicera japonica in antioxidant stress, the active ingredients in Lonicera japonica break the Keap1-Nrf2 protein-protein interaction in cells, activate Nrf2 pathway, and target binding Keap1 protein, release Nrf2 protein, and activate the mode of antioxidant enzyme to play the role of antioxidant.The present application first proves by experiment that the active ingredients in Lonicera japonica target binding Keap1 protein and break Keap1-Nrf2 PPI, release Nrf2 protein, and activate the expression of downstream antioxidant enzymes (such as NQO1), so as to realize the effect of antioxidant.

The invention discloses an application of a compound composition containing astragaloside IV in preparation of a medicine for resisting plateau fatigue, and the compound composition containing astragaloside IV is prepared from the following raw materials in parts by weight: 500 to 2000 parts of astragaloside IV, 5000 to 20000 parts of ginsenoside Rg1, 2 to 5 parts of vitamin D3 and 30000 to 150000 parts of puerarin. Animal experiments prove that the compound composition can relieve plateau exercise fatigue, improve the hypoxia tolerance, exercise fatigue resistance and oxidative stress resistance of a body and improve the comprehensive exercise performance of the body. According to the invention, the defect of single-component efficacy is overcome, a compound preparation is designed in a targeted manner, an innovative and feasible compound combination is provided, and a new scheme is provided for promoting athletic performance under plateau hypoxia.

The invention discloses a milk-derived active peptide DDLCDYWIR with strong antioxidation and immunoregulation as well as a preparation method and application thereof, and relates to the field of active peptides. According to the invention, lactoferrin is used as a raw material, and after protease enzymolysis and lactobacillus paracasei fermentation treatment are carried out, a novel milk-derived active peptide is screened from the product. Experiments prove that the active peptide shows excellent antioxidant activity and immune regulation effect, not only can enhance the immune function of the body, but also can effectively resist oxidative stress and play a role in delaying senescence. Meanwhile, the active peptide is derived from a natural milk source, is not added with any chemical substance, has good biocompatibility, and can be widely applied to preparation of antioxidant and immunomodulatory products or products with the antioxidant and immunomodulatory functions.

The invention relates to the technical field of traditional Chinese medicine preparations, and discloses a preparation method of a liver-protecting and hangover-alleviating formula containing radix puerariae, turmeric, hovenia dulcis thunb and penthorum chinense pursh, and the liver-protecting and hangover-alleviating formula is prepared from the following raw materials in parts by weight: 20-40 parts of radix puerariae, 15-30 parts of turmeric, 10-25 parts of hovenia dulcis thunb and 10-25 parts of penthorum chinense pursh. According to the preparation method of the liver-protecting and hangover-alleviating radix puerariae, turmeric, hovenia dulcis thunb and penthorum chinense pursh, based on the theory of traditional Chinese medicine and modern pharmacological research, radix puerariae, turmeric, hovenia dulcis thunb and penthorum chinense pursh are scientifically matched, and radix puerariae has the effects of resisting oxidation and promoting alcohol metabolism; turmeric is anti-inflammatory and anti-oxidative stress; hovenia dulcis thunb inhibits alcohol absorption and protects liver cell membranes; penthorum chinense pursh has the effects of detoxifying, resisting inflammation and protecting the liver, the four medicines are combined to achieve a multi-target and multi-path synergistic effect, the powerful effects of protecting the liver and relieving alcoholism are jointly achieved, and the effect is better than that of a single medicinal material or a simple combination.

The application provides a preparation method of human collagen and application of the human collagen in anti-aging, and the amino acid sequence of the human collagen is shown as SEQ ID NO:1; the application is based on a natural collagen structure, provides a fusion sequence structure, and adjusts the proportion of individual amino acids; the collagen can promote skin cell growth and hyaluronic acid secretion, counteracts skin aging caused by ultraviolet radiation, inhibits expression of inflammatory factors, and counteracts oxidative stress.

The application provides anti-aging use of cromolyn and derivatives thereof. Specifically, the application provides use of cromolyn or a kind of cromolyn N-ethyl-N-phenyl acrylamide derivative in preparation of an anti-aging composition or preparation. The cromolyn and derivatives thereof of the application can prolong the lifespan of nematodes; the derivative JM03 can also significantly prolong the antioxidant stress resistance and osmotic stress resistance of nematodes. Therefore, the cromolyn and derivatives thereof of the application have application prospects in the aspect of anti-aging.

The invention belongs to the technical field of biological agents, and particularly relates to application of Roseburia inulinivorans in preparation of a medicine for treating and / or preventing enteritis. The invention finds that the intestinal bacteria Roseburia inulinivorans can improve ulcerative colitis by adjusting the steady state of microorganisms, protecting the integrity of intestinal barriers and maintaining the steady state of immunity. According to the application, the curative effect of Roseburia inulinivorans on colitis is comprehensively evaluated, and the Roseburia inulinivorans has the functions of resisting inflammation, recovering immune balance, resisting oxidative stress, resisting pyroptosis, regulating bile acid metabolism, relieving endothelial dysfunction and intestinal ecological dysfunction and the like, and particularly, the Roseburia inulinivorans can achieve the treatment effect the same as that of a clinical conventional medicine mesalazine. And meanwhile, the Roseburia inulinivorans has no side effects, so that the Roseburia inulinivorans have no side effects. Therefore, the Roseburia inulinivorans is very likely to become a good candidate drug for improving the ulcerative colitis.

The invention discloses metal polyphenol network hydrogel containing kaempferia galanga extracellular vesicles as well as a preparation method and application thereof, and aims to provide metal polyphenol network hydrogel which has good biocompatibility and can promote hair follicle microangiogenesis, remove excessive ROS (reactive oxygen species) of hair follicles, reduce apoptosis of hair follicle cells, promote hair growth and promote hair growth. The prepared hydrogel has good injectability and anti-inflammatory and anti-oxidative stress capabilities, can be used for preparing anti-inflammatory and anti-oxidative products, and can also be used for preparing metal polyphenol network hydrogel of products for treating alopecia. The preparation method comprises the following steps: uniformly mixing a kaempferia galanga extracellular vesicle solution and a sodium alginate solution, then adding a tannic acid solution, uniformly mixing, finally adding a zinc chloride solution, uniformly mixing, standing at normal temperature to form gel, freezing, and unfreezing at normal temperature after freezing is finished, thereby obtaining the kaempferia galanga extracellular vesicle gel. The invention belongs to the technical field of biological medicine.

The application discloses a selenium-rich compound bacteria fermentation product and application thereof in photodamage repair, inoculates animal bifidobacterium H15 and lactobacillus coryniformis ES23 after compounding into MRSc culture medium containing sodium selenite, carries out anaerobic fermentation culture, cell wall breaking, and filters and sterilizes to obtain the selenium-rich compound bacteria fermentation product. The selenium-rich compound bacteria fermentation product can efficiently remove free radicals, has good antioxidant stress resistance, the ability of repairing UVB photodamage cells and high biological safety.

The present application belongs to the field of microbial engineering, and discloses a broken wall red phaffia yeast feed additive, a preparation method and application thereof. The preparation method of the broken wall red phaffia yeast feed additive comprises the following steps: strain culture: inoculating red phaffia yeast into YM liquid culture medium to obtain seed liquid through culture; fermentation expansion culture: transferring the seed liquid into new YM liquid culture medium to carry out fermentation culture, and centrifuging to obtain bacterial body precipitate; wall breaking treatment: adding hydrochloric acid into the bacterial body precipitate, heating, cooling, centrifuging to obtain precipitate; freeze-drying to obtain the broken wall red phaffia yeast feed additive. The broken wall red phaffia yeast feed additive, the preparation method and application thereof can significantly improve the growth rate and survival rate of seedlings, improve the antioxidant stress resistance and immunity of seedlings, and enhance the stress resistance of seedlings.

The invention relates to the technical field of cosmetics, in particular to a composite peptide-containing composition for increasing skin elasticity and a preparation method thereof, and the composite peptide-containing composition comprises composite peptide and composite plant extract; the composite peptide is prepared from the following raw materials: oligopeptide-4, palmitoyl dipeptide-7, tripeptide-10 citrulline, and an antioxidant. The composite plant extract is prepared from the following raw materials: plumeria rubra flowers, chayote fruits and plantain fruits. Different peptides are selected to form the composite peptide raw material for improving the skin elasticity, so that multi-channel improvement on the skin elasticity can be adaptively realized, and the effect of improving the skin elasticity of a finished product is effectively improved. The white plumeria rubra flowers, the chayote fruits and the big banana fruits are blended and extracted, the active ingredients of the obtained compound extracting solution are diversified and complementary in effect, the compound extracting solution can have different effects of resisting oxidative stress reaction, removing various free radicals and reducing attack of reactive oxygen free radicals on skin collagen, and the remarkable anti-oxidation effect is achieved through the synergistic effect.

Provided are previously unknown novel uses of 5-deazaflavin derivatives and related compounds.　Provided are: an antioxidative stress agent containing, as an active ingredient, a 5-deazaflavin compound represented by general formula (I), a pyridodipyrimidine compound represented by general formula (II), a deazaflavinotestosterone compound represented by general formula (III), or a deazaflavinocholesterol compound represented by general formula (IV); and a cosmetic, a quasi-drug, and a pharmaceutical each containing any of the compounds.

The invention discloses a Chinese herbal medicine composition with an effect of relieving plateau exercise fatigue and application thereof, and the Chinese herbal medicine composition is prepared from the following raw materials in parts by weight: 3-27 parts of an acanthopanax senticosus extract, 3-15 parts of a radix salviae miltiorrhizae extract, 5-15 parts of a moringa oleifera leaf extract, 3-15 parts of a radix puerariae extract, 3-10 parts of a turmeric extract and 3-9 parts of an tangerine pith extract. All the extracts are water extracts. The composition can relieve plateau exercise fatigue, improve the hypoxia tolerance, exercise fatigue resistance and oxidative stress resistance of the body, and improve the comprehensive exercise performance of the body.

The application discloses a compound feed for high-salt-alkaline water aquaculture of large-mouth black bass and a preparation method thereof. The raw material composition comprises 42-46 parts of fish meal, 6-8 parts of pork meal, 5-7 parts of casein, 13-17 parts of soybean meal, 10-14 parts of wheat flour, 4.5-8.5 parts of fish oil, 0.5-2 parts of sodium carboxymethyl cellulose, 1-3 parts of Ca(H2PO4)2, 6-8 parts of microcrystalline cellulose, 0.5-2 parts of premix, and 0.1-1 part of choline chloride. When the fat content is 10wt%-12wt%, the compound feed can improve the lipid metabolism of serum and liver of the large-mouth black bass, protect the liver, improve the immune and antioxidant enzyme activity, reduce the tissue damage induced under high-salt-alkaline water aquaculture, improve the intestinal flora disorder of the large-mouth black bass by increasing the number of intestinal probiotics such as phocaebacterium, and further improve the growth performance, immunity and antioxidant stress capacity of the large-mouth black bass under high-salt-alkaline stress.

The invention relates to the field of infant food, in particular to an anti-oxidative stress composition and application thereof to improvement of neonatal necrotizing enterocolitis. The composition is prepared from the following components: neutral oligosaccharide and sialylated oligosaccharide. The neutral oligosaccharide is 3-fucosyllactose, and the sialylated oligosaccharide is 3 '-sialic acid lactose. The invention provides a composition capable of relieving neonatal necrotizing enterocolitis. Through combination of the 3-fucosyllactose and the 3 '-sialic acid lactose, related symptoms of the neonatal necrotizing enterocolitis can be improved by inhibiting a TLR4 / NF-kappa B inflammation pathway, improving cell oxidative stress, protecting cell barriers and reducing cell apoptosis, and the 3-fucosyllactose and the 3'-sialic acid lactose have important application value in the field of functional foods or formula foods.