191 results about "Antioxidative stress" patented technology

The invention relates to a method for preparing a chitosan-silk fibroin composite nano-fiber multifunctional patch for promoting myocardial tissue regeneration and monitoring stem cells. The method comprises the following steps: preparing a cellulose nano-fiber basal plate by an electrostatic spinning technology; alternately assembling positively charged chitosan (CS) and negatively charged silk fibroin (SF) to the surface of nano-fibers layer by layer by adopting a layer-by-layer assembly technique, and assembling 5.5-10.5 layers to form a CS-SF composite nano-fiber membrane; and planting seed cells of adipose tissue-derived stromal cells or cardiac progenitor cells labeled by green fluorescent protein and firefly luciferase on the surface of the CS-SF composite nano-fiber membrane, preparing the patch by three-dimensional co-culture. The patch has excellent biocompatibility, can be used as a cell vector, has an effect of resisting oxidative stress to improve the survival rate and treatment efficiency of stem cells, and is capable of evaluating the number, distribution and function states of transplanted stem cells, effectively preventing occurrence of post-myocardial infarction heart failure and reducing the death rate of ischemic cardiomyopathy.

The invention discloses application of bioflavonoids or polyphenol compounds to preventing and treating Parkinson diseases. The bioflavonoids or polyphenol compounds can be selected from kaempferol-3-O-rutinoside, dehydrated carthamin yellow B, 6-hydroxyl kaempferol-3, 6-dioxygen glucoside, (2S)-4', 5-dihydroxyl-6, 7-dioxygen flavonone glucoside, rutin, quercetin, vanillina or isovanillin. Through large amount of experiments, the bioflavonoids or polyphenol compounds have activity of interacting with protein DJ-1 related to the Parkinson diseases. Through further experiments, the bioflavonoids or polyphenol compounds have pharmaceutical activities for resisting oxidative stress, preventing apoptosis of PC12 cells or primary nerve cells induced by the oxidative stress, inhibiting active oxygen generation in cells, improving activity of tyrosine hydroxylase, and the like, and can be used for preventing or treating the Parkinson diseases.

The invention is directed to methods for identifying test substances useful for the prevention or treatment of diseases involving an oxidative stress. The methods involve screening assays, including high throughput screening techniques, in which the test substances are tested for their ability to promote resistance to oxidative stress by activating one or more points of the integrated stress response pathway, while not causing stress.

The present invention discloses a fermented selenium-rich plant feed additive resistant to pig heat stress and a preparation method of the feed additive comprises the following three steps: (1) preparation of selenium-rich yeast culture medium; (2) preparation of traditional Chinese medicine fermentation culture medium; and (3) traditional Chinese medicine fermentation. The plant feed additive is characterized in that: the traditional Chinese medicine fermentation culture medium obtained in the step 2 includes (2.1) preparation of Chinese herbal medicine probiotics and (2.2) inoculation steps. The designed fermented selenium-rich plant feed additive is a pig heat stress resistant novel feed additive which is green and safe, contains traditional Chinese medicines, and combines organic selenium. The feed additive fully integrates the physiological characteristics of heat-stressed pigs, uses anti-oxidative stress as main features, can significantly improve the pig weight gain and feed-weight ratio in a high-temperature environment, and enhance pig anti-oxidation resistance and immune function after being fed, effectively plays the roles of pig stress resistance, and has non-toxic or side effects and no harmful substance residues after a long-term use.

The invention relates to anti-oxidative stress normal temperature preservation diluent and liquid for porcine seminal fluid and application of the normal temperature preservation liquid. The preservation liquid contains a diluent and dilution liquid. Each 1L of the preservation liquid contains the following diluent components by weight: 1g-6g of bovine serum albumin, 5g-6g of trihydroxymethylaminomethane, 0.1g-0.15g of cysteine, 6g-10g of sodium citrate, 2.4g-3g of a chelating agent, 0.17L-0.22L of yolk, 0.08L-0.12L of mycose, 1g-2g of sodium hyaluronate, 0.5g-1.1g of potassium chloride, 0.5mmol-1mmol of an antioxidant, 0.1g-0.2g of a bactericide, 0.2g-1g of L-carnitine and 0.5g-2g of tea polyphenol. In an application process, 3mL-5mL of the preservation liquid is added into each 1mL of the porcine seminal fluid so as to realize the normal temperature preservation of the porcine seminal fluid. Compared with the prior art, the preservation diluent and the preservation liquid have the advantages that the components are mutually matched, so that porcine semen cells are protected, an appropriate living environment is provided for porcine semen, the normal physiological activity of the porcine semen cells is guaranteed, excess free radicals are effectively removed, the oxidative stress resistance and preservation effect of the porcine seminal fluid are improved, the survival rate of the porcine semen is guaranteed, and furthermore, the breeding rate and culture benefits of sows are increased.

A lycopene-containing feed additive relates to a feed additive, and solves the problems of chemical residue, drug resistance and toxic and side effects of the prior food additives. The lycopene-containing feed comprises lycopene, soybean oil and soybean meal, and has the advantages that the feed additive is safe without toxic and side effects, and can effectively improve antioxidant stress ability, immunologic function and muscle quality of livestock and poultry and remarkably reduce fat deposition in vivo.

The invention is applicable to the technical field of nutritional health-care food, and provides a skin beautifying composition based on a collagen structure, enzyme inhibition and oxidative stress as well as a preparation method. The skin beautifying composition comprises an anti-sugar and anti-aging component, an anti-enzyme activity component and an anti-oxidative stress component, wherein the anti-sugar and anti-aging component comprises 0.01-0.15 part of sodium hyaluronate and 1-5 parts of collagen peptide, the anti-enzyme activity component comprises 0.01-0.5 part of a pomegranate extract and 0.005-0.25 part of N-acetylneuraminic acid, and the anti-oxidative stress component is prepared from 0.06-0.3 part of a grape seed extract and 0.02-0.1 part of strawberry seed powder. The composition disclosed by the invention can improve skin problems such as external aging saccharification, wrinkles, dark skin color and melanin accumulation, continuously regulate biological metabolism of an internal body, enhance the activity of antioxidant enzyme, inhibit oxidative stress reaction, eliminate the level of active oxygen, prevent UV (ultraviolet) damage, increase cell viability and repair power, and protect the dermis layer to achieve the effects of skin whitening, oxidation resistance and aging resistance.

A method for identifying modulators of the Keap1-NrG-ARE pathway is described. In particular, an assay is described that identifies molecules that inhibit the binding of a labeled Nrf2 peptide with the kelch domain of the Keap1 protein. Molecules that inhibit the binding are activators of the Keap 1-Nrf2-ARE pathway. Activation of the Keap 1-Nrf2-ARE pathway may result in an increased accumulation of Nrf2 and the subsequent induction of protective enzymes, for example, the phase 2 detoxification enzymes. Activators of the Keap1-NrG-ARE pathway are useful for combating oxidative stress-related disorders, such as those associated with cancer, emphysema, Huntington's disease, light-induced retinal damage, and stroke.

The invention discloses a feed formula for resisting the heat stress of broilers. According to the feed formula, dry fly maggot powder in an amount which is 1 to 3 percent based on the weight of a basal feed and a ginkgo leaf extract Egb761 in an amount which is 0.1 to 0.3 percent based on the weight of the basal feed are added into the basal feed. According to the feed formula, the fly maggot powder is rich in protein and essential amino acid, and can enhance the disease-resistant and stress-resistant capacity of the broilers while improving the growth performance of the broilers; and ginkgo leaves and the ginkgo leaf extract thereof have rich ginkgo flavone and terpene lactones, and have an anti-oxidative stress effect. In addition, the ginkgo leaves and the ginkgo leaf extract thereof also improve the growth capacity and disease-resistant capacity of the broilers and improve the quality of chicken.

The present invention provides methods for identifying agents that increase lifespan and increase resistance to oxidative and / or electrophilic stress. Also provided are methods for identifying biomarkers of longevity or identifying pathways governing longevity in response to phosphatidylinositol 3,4,5-triphosphate signaling.

The invention provides bacillus coagulans E21 capable of improving oxidative stress resistance of livestock and poultry animals after toxic attack, and the application thereof. The patent preservationnumber of the strain is CGMCC NO.13044. The strain is derived from the intestinal tract of a healthy animal; a series of experiments prove that the strain has high heat resistance, acid resistance, cholate tolerance, stability and capability of producing high-yield L-lactic acid; furthermore, the zymophyte quantity of the bacillus coagulans reaches to 10 billion CFU / mL, and the bacillus coagulanscan be applied to feed additives. The bacillus coagulans can improve the oxidation resistance of white-feather broiler chickens after toxic attack, the white-feather broiler chickens are subjected totoxic attack by aquacide, the organism is in an oxidative stress state, the morphological structure of jejunum is damaged, and the intestinal barrier function is influenced. Due to the addition of the bacillus coagulans, the oxidation resistance of the white-feather broiler chickens after toxic attack can be improved, the morphological structure of the jejunum is recovered, balance of inflammation-causing factors and inflammation-inhibiting factors is promoted, and the intestinal barrier function is protected.

The invention discloses a cerebroprotein hydrolysate and a preparation method thereof, and belongs to the technical field of medicines. The preparation method comprises the following step: carrying out enzymolysis on an animal brain tissue by adopting pepsase and compound flavourzyme in a mass ratio of (6:1)-(2:1) at 37 DEG C under a corresponding enzymolysis pH value controlled at 1-5 for 2-10 hours, thereby obtaining the cerebroprotein hydrolysate, wherein cerebroprotein hydrolysate is a composition of oligopeptide with molecular weight of 1000 and polypeptide. And moreover, macromolecule impurities are removed by centrifuging, and impurities such as pyrogen are filtered and removed by a nanofiltration membrane. The finally obtained cerebroprotein hydrolysate has apyrogeneity, high oxidative-stress-resisting activity and good repair capacity on nerve cells.

Provided is a biomarker that enables easy and rapid detection of oxidative stress on a living organism and enables prevention of tissue damage or cell necrosis by drug administration, and which is a powerful marker for the study of toxicity and pharmacokinetics of various agents. Oxidative stress is determined by measuring blood concentration of ophthalmic acid, which is a substance that varies in blood depending on the variation of reduced glutathione (GSH) concentration in a biological sample with the use of an analyzer such as a capillary electrophoresis-mass spectrometer. Further, an anti-oxidative stress agent is screened by administering an anti-oxidative stress candidate agent to a non-human animal under oxidative stress conditions, measuring blood concentration of ophthalmic acid, and evaluating the degree of decrease in the ophthalmic acid concentration.

The invention discloses application of clostridium butyricum in preparation of medicines for treating and / or preventing acute liver injury, belonging to the field of medicines. According to the application, the clostridium butyricum is capable of relieving oxidative stress response in the acute liver injury by improving the activity of superoxide dismutase and catalase as well as reducing the content of malonaldehyde in the liver tissue, and can reduce the accumulation of IL-1beta, IL-6 and TNF-alpha in the liver tissue at the same time; therefore, the clostridium butyricum has functions of resisting oxidative stress and resisting inflammatory response, and is capable of effectively treating liver injury so as to restore the normal liver functions. Furthermore, the main metabolite of the clostridium butyricum is butyric acid, so that the clostridium butyricum is beneficial to the protection of the liver.

The invention belongs to the technical field of special medicinal food production, and specifically relates to prebiotics-containing formula food for special medicinal use in diabetes and a preparation method thereof. The prebiotics-containing formula food is prepared from the following components in percentage: 15 to 40 percent of yam flour, 11 to 15 percent of whey protein, 10 to 14 percent of resistant dextrin, 8 to 12 percent of walnut powder, 8 to 12 percent of xylitol, 12 to 20 percent of composite peptide powder, 5 to 9 percent of L-arabinose, 5 to 9 percent of inulin, 4 to 8 percent ofxylooliosaccaride powder 95, 0.080 to 0.1 percent of decavitamin, 0.4 to 0.45 percent of composite minerals, 2 to 6 percent of a momordica charantia extract and 1 to 5 percent of a herba gynostemmatis extract. The prebiotics-containing formula food disclosed by the invention not only is capable of effectively reducing blood glucose, but also has the functions of increasing the anti-oxidative stress ability of diabetic mice and reducing the lipotoxicity, and the function of protecting pancreatic tissues can be achieved.

A drug compound preparation for treating Alzheimer's disease includes the main components of epigallocatechin gallate and vitamin E. Oil-soluble vitamin E is mixed with 1 part by volume of edible oil solution, and water-soluble EGCG is mixed with 2 parts by volume of distilled water; the two liquids are mixed and then added with an emulsifier in the weigh 10% of the edible oil, so as to form an oil-water suspension with mass fraction of EGCG of 0.02-0.1% and mass fraction of vitamin E of 0.3%. The invention has the advantage that the drug compound preparation has significant improvement on learning and memory impairment in an Alzheimer's disease mouse model induced by D-galactose, and can reduce the deposition of A beat 1-40 in the brain; and the drug compound preparation reduces antioxidation stress effect, anti-apoptosis effect and anti-inflammatory effect by increasing antioxidant enzymes, so as to exert the effect in resisting Alzheimer's disease. The drug compound preparation has simple composition, clear efficacy and easy preparation, can effectively play the roles of improving the behavior and learning and memory functions of patients, and does not generate other adverse reactions in large dose application.

Provided herein are pharmaceutical compositions and methods for treating disorders characterized by reduced expression of anti-oxidative stress enzymes in a subject. In one aspect, the present invention provides a method for inducing expression of anti-oxidative stress enzymes in a subject in need thereof. The method includes administering to the subject a therapeutically effective amount of a demethylating agent. The demethylating agent includes a phytochemical that induces expression of Nrf2 and Nrf2-mediated genes expressing anti-oxidative stress. Such phytochemicals include indoles, such as 3,3′-diindolylmethane (DIM) and indole-3-carbinol (I3C), and isothiocyanates, such as phenethyl isothiocyanate (PEITC) and sulforaphane (SFN).

The invention discloses a preparation method of feed capable of protecting the liver of GIFT oreochromis niloticus and boosting the immunity of the GIFT oreochromis niloticus and a feeding method of the feed. The preparation method comprises the steps of feed preparation and chitosan addition. The feeding method of feed comprises the step of feed feeding. After the GIFT oreochromis niloticus is fed for 9 weeks by adopting the feeding method, the immunity index of the GIFT oreochromis niloticus is obviously increased, the liver stress is decreased, the oxidative stress resistance capacity of the liver is enhanced, the activity of superoxide dismutase, catalase, glutathione and glutathion peroxidase in the liver is obviously improved, and the level of the malonaldehyde is lowered; the level of glucose, cholesterol and low-density cholesterol in serum is obviously lowered; the level of alkaline phosphatase and high-density cholesterol is obviously increased. The chitosan added to the feed is metabolites generated by fermentation of microorganisms without antagonism and is small in molecular weight, easy to absorb, low in cost and relatively easily available. After the feed is taken by the GIFT oreochromis niloticus, the feed is easy to digest and absorb by the GIFT oreochromis niloticus, and residues are barely remained in bodies; the feed has a very good immunopotentiator effect and meets the requirements on novel green healthy additives.

The invention provides an application of enhancing antioxidation of an oocyte cultured in vitro by a Peroxiredoxin 4 protein. The maturing rate of the immature oocyte cultured in vitro can be improvedby improving the resistance of particle cells in oxidative stress and improving the antioxidation of the oocyte. Aging of the oocyte is also delayed through an antioxidant stress mechanism in the cell, and the fertility rate and the clinical pregnancy rate of the oocyte in an assisted breeding process are improved.

The invention relates to the technical field of food processing and discloses noodles capable of reducing blood sugar and regulating blood lipid and a preparation method. The noodles are prepared from raw materials in parts by weight as follows: 100 parts of high gluten wheat flour, 1-9 parts of tartary buckwheat flour, 1-9 parts of yam flour, 10-30 parts of mulberry leaves, 10-25 parts of radix puerariae and 10-30 parts of bitter gourds. The active ingredients of the mulberry leaves, the radix puerariae, the bitter gourds and the like are extracted and mixed with the high gluten wheat flour, the tartary buckwheat flour and the yam flour for preparation of the noodles. Animal experiments and feeding trials prove that the noodles have significant effects of reducing blood sugar and regulating blood lipid on a diabetes model mouse and can effectively control postprandial blood sugar rise of diabetics and obviously improve the oxidative stress resistant level. The noodles can be used as safe staple food of the diabetics to control postprandial blood sugar rise and improve the life quality of the diabetics and can also be used as healthcare food for preventing diabetes to be eaten for a long term.

Activation of HIV-1 replication causes oxidative stress, which in turn potentiates HIV-1 replication. The common basis for the compounds of the present invention is: A) the capacity of reactivating HIV-1 from latency, and B) the ability to counteract the cellular machinery which activates in order to limit the effects of oxidative stress. In this way, oxidative stress can be potentiated and a “chain reaction” is sparked. This “chain reaction” induces a more efficient reactivation of HIV-1 from latency and, in some cases, induces selective killing of the infected cells. Actions A) and B) can either be carried out by one drug exerting both effects, or obtained by the combined use of distinct drugs. There are two main cellular machineries counteracting oxidative stress, i.e. the thioredoxin (Trx) thioredoxin reductase (TrxR) system and glutathione. Herein, we present drug strategies capable of exerting action B) by blocking either of the two machineries.

The invention discloses a hydrogel microcapsule for encapsulating a nicotinic acid metal organic framework and a preparation method and application thereof. The preparation method of the hydrogel microcapsule for encapsulating the nicotinic acid metal organic framework comprises the steps that a nicotinic acid solution is used as an inner phase 1; a mixed solution of a zinc acetate solution and acopper acetate solution is used as an inner phase 2; a sodium alginate solution is used as an outer phase; and a microfluidic device is used for spraying mixed liquid drops of the inner phase 1 and the inner phase 2 wrapped by the outer phase into a calcium chloride solution to prepare the hydrogel microcapsule for encapsulating the nicotinic acid metal organic framework. The hydrogel microcapsulefor encapsulating the nicotinic acid metal organic framework, which is prepared by the invention, has ideal controlled release effect, antibacterial effect and anti-oxidative stress effect, and can be used for preparing wound healing materials or medical instruments.

The invention discloses an application of a safflower extract to prevention or treatment of a neurodegeneration disease. As proved by a large quantity of experiments, the safflower extract has a remarkable oxidative stress resisting function and can be used for effectively improving the behavioristic representation of a mouse suffering from chronic Parkinson's disease C57 induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and protecting the integrity of dopaminergic neuron form. As proved by an experimental result, the safflower extract has a definite pharmacological activity on the prevention or treatment of the neurodegeneration disease.

Antioxidative stress compositions, methods of using antioxidative stress composition and methods of preparing antioxidative stress composition compositions are described. The antioxidative stress compositions may be chemopreventive and / or immunomodulatory. Resveratrol, genistein, ellagic acid, curcumin and quercetin may be included in the antioxidative stress composition compositions individually or in any combination.

The invention belongs to the field of biological medicine and particularly relates to an application of a FoxO1 gene in drug preparation. According to the invention, the function of the FoxO1 gene in osteoblast proliferation differentiation under high sugar condition is researched so as to research the function of the FoxO1 gene in bone injury and osteoporosis induced by diabetes, in order to provide theoretical basis and experimental data for clinical treatment of diabetic bone injury and osteoporosis disease. The invention verifies that the FoxO1 gene can promote osteoblast proliferation, FoxO1 is used as a factor used for mainly regulating and controlling bone mass and has an antioxidant stress function on osteoblast, the FoxO1 gene generates a certain protection function on osteoporosis no matter in environment with increased oxidative stress level caused by ageing or diabetes or high blood sugar, and provides a new target for research and development of new anti-osteoporosis drugs.

The invention relates to application of an epimedium total flavone in intervention of intestinal tract barrier dysfunction, in particular to application of the epimedium total flavone in preparation of medicine for restraining intestinal tract barrier dysfunction. The epimedium total flavone regulates the aging intestinal tract barrier dysfunction through promoting expression of anti-oxidative stress and longevity related signal channels. The promotion of cell tight junction protein expression includes promotion of intestinal duct claudin-1 and occluding expression.

The invention aims to provide a feed additive for improving the anti-stress capability of large yellow croakers. The feed additive comprises procyanidine and a vitamin C additive, wherein the mass ratio of procyanidine and the vitamin C additive is preferably (0.5-2):1. The additive amount of procyanidine in the anti-stress additive of large yellow croakers is 100-300mg / kg, and the additive amount of vitamin C phosphate is 500-600mg / kg. The procyanidine and the vitamin C additive combined in the proportion can be used for improving the anti-stress capability and anti-trawling stress capability of the large yellow croakers and does not generate obvious side effects on livers of large yellow croakers.

The invention features a method for inhibiting or reducing the deleterious effects of tobacco smoking including increased incidence of cancers, cardiovascular diseases, various lung disorders and the oxidative effects. The method includes the incorporation of an effective amount of a compound or a standardized plant extract that is that comprises an inhibitor of antioxidant stress due to the induction of phase 2 enzymes regulated by Nerf2 into tobacco or a tobacco product.

The invention discloses a fermented selenium-rich vegetable feed additive. A preparation method of the fermented selenium-rich vegetable feed additive comprises the following three steps: (1) preparation of a selenium-rich yeast culture solution, (2) preparation of a traditional Chinese medicine fermentation culture medium and (3) traditional Chinese medicine fermentation. The preparation method is characterized in that the step (2) comprises the following steps: (2.1) preparation of a Chinese herbal medicine probiotics culture medium and (2.2) inoculation. The fermented selenium-rich vegetable feed additive designed by the invention is a novel environmentally-friendly safe feed additive which contains fermented traditional Chinese medicines and organic selenium and is capable of resisting heat stress of pigs. By adequately combining with physiological characteristics of heat stress pigs and taking oxidative stress resistance as the main characteristic, after pigs are fed with the additive, the weight increment and feed to gain ratio of the pigs in a high-temperature environment can be remarkably improved, the oxidation resistance and immunologic functions of the pigs can be enhanced, and the stress resistance of the pigs is effectively exerted; and after the additive is used for a long time, no toxic and side effects are generated, and no harmful substance is remained.

The invention relates to a bismuth sulfide-zinc protoporphyrin composite material with a tumor photodynamic therapy (PDT) property under excitation of near-infrared light as well as a preparation method and application and belongs to the field of composite materials, aiming at solving the problem that the PDT efficiency is relatively low, caused by photoelectron-hole compounding in a photodynamictherapy process of bismuth sulfide and an anti-oxidization stress capability of heme oxygenase (HO-1) in cells. The composite material is the bismuth sulfide-zinc protoporphyrin composite material which is obtained by synthesizing a polyN-isopropylacrylamide-acrylamide copolymer modified bismuth sulfide nano-material and then carrying out condensation reaction of carboxyl and amino. The prepared composite material has the effect of enhancing the photodynamic therapy efficiency through two ways of inhibiting the activity of the heme oxygenase and promoting electron-hole separation; meanwhile, the material provided by the invention has good biocompatibility, light stability, CT (Computed Tomography) imaging capability and enhanced PDT efficiency; a thought is provided for designing a novel nano diagnosis and treatment integrated system and the material has important meaning on early-stage diagnosis and treatment of cancers.