153 results about "Coomassie Brilliant Blue" patented technology

The invention discloses a method for preparing water soluble graphene. The method for preparing the water soluble graphene comprises the following steps of: oxidizing graphite into graphite oxide; then adding coomassie brilliant blue; performing a reaction of the coomassie brilliant blue and the graphite oxide by ultrasound; adding a reducing agent; and performing a reaction to obtain the water soluble graphene. The solubility of the water soluble graphene can reach 1 to 1.5mg / mL, the electrical conductivity of the water soluble graphene can reach 1 to 2S m-1, and the water soluble graphene can be stably stored for 3 to 6 months without precipitates.

The invention discloses a detection method for trace protein of a Chinese medicinal injection, which is used for detecting the protein impurities of the Chinese medicinal injection so as to control the quality of the Chinese medicinal injection. The detection method is characterized by comprising the following steps of: 1) applying a Chinese medicinal injection sample on a protein specific adsorption film; 2) washing the sample application film with organic solvent-containing solution to wash away the natural color of dots of the Chinese medicinal injection; and 3) subsequently performing coomassie brilliant blue staining on the sample application film. If the protein contained in the Chinese medicinal injection is no less than 12mu g / ml, stable and differentiable blue can be displayed on the film. The detection sensitivity of the method is higher than that of a sulfosalicylic acid precipitation method, and the detection method has high anti-interference capacity and is suitable for the detection of the trace protein of all the Chinese medicinal injections.

The invention discloses a Coomassie brilliant blue dyeing method and its special gel stationary liquid and coloring agent. It includes the following steps: putting SDS-PAGE glue into the gel stationary liquid for 0.5-2.0h at normal temperature; adding the glue in acetate solution with 0.5-3.0% volume percent concentration to extend for 1.0-3.0h at normal temperature; adding it to the Coomassie brilliant blue coloring agent to dye for 8-24h at normal temperature; add it to decolorizing solution I to decolorize for 1-3h; then adding to decolorizing solution II to decolorize to clear. The invention can detect SDS-PAGE glue protein at nanogram.

The invention provides a fluorescence contrast agent. The fluorescence contrast agent comprises gold nanoclusters, antibodies of tumor specificity markers and coomassie brilliant blue, wherein the gold nanoclusters and the antibodies of the tumor specificity markers are connected in a click-chemistry mode; and the coomassie brilliant blue is combined with the gold nanoclusters connected with the antibodies of the tumor specificity markers. The fluorescence contrast agent provided by the invention has the characteristic of naked-eye visualization. In addition, the invention also provides a preparation method of the fluorescence contrast agent; and the method is simple in step and is suitable for industrial production.

The invention relates to a method for detecting trace protein of a Chinese medicine injection, which is used for detecting protein impurities in the Chinese medicine injection to control the quality of the Chinese medicine injection. The method is characterized by comprising the following steps of: 1) soaking a protein specific adsorption film in Chinese medicine injection solution to be detected; 2) washing the protein adsorption film by using solution containing an organic solvent to reduce color interference of the Chinese medicine injection; and 3) performing comassie brilliant blue colordevelopment on the protein adsorption film. If the protein contained in the Chinese medicine injection is not lower than 0.5 mu g / ml, the protein can display a stable and distinguishable blue color on the film. The method has high detection sensitivity, and simultaneously has strong interference resistance, so the method is suitable for detecting the trace protein of all Chinese medicine injections.

The invention discloses a coomassie brilliant blue staining solution and a staining method. The coomassie brilliant blue staining solution comprises 0.1-10 percent by volume of acid, 1-15 percent by volume of ethyl alcohol, 10-50g / L of soluble starch and 20-1000mg / L of coomassie brilliant blue aqueous solution. The staining solution does not contain reagents harmful to human bodies and is environmental friendly. The staining method using the staining solution has the beneficial effects that due to addition of the soluble starch, the staining background of gel can be reduced, a protein band can be specifically stained, the sensitivity of staining is effectively improved, and therefore, the whole staining process of the protein band can be completed within 20 minutes by using the staining method, the steps such as gel fixation, sensitization and destaining in a conventional dyeing process are omitted, operating steps are greatly simplified, and the dyeing time is saved; the staining method has the advantages of short cycle, high sensitivity and small background interference.

The invention relates to a method to test tumor relative marking material from esophageal patient blood serum that uses blood serum as molecule probe. The Ku70 albumen would used as the new esophageal patient marking material. It is relative to early stage esophageal.

A method for measuring protein in sludge according to the coomassie brilliant blue method belongs to the field of influencing factors in municipal sewage treatment. After being combined with protein, coomassie brilliant blue G-250 becomes cyan, the protein-pigment combined material is highest in light absorption value under a 595nm wavelength condition, and the light absorption value is in direct proportion to protein content, the method can be used for quantitative measurement of protein. The method particularly relates to the following steps: reagent preparation, standard curve measuring, sludge pretreatment, protein extraction and measuring, and the like. The method is a simple and efficient method for measuring the protein content in sewage.

The invention discloses a method for classifying artificial wetland obstructions and extracting components of the artificial wetland obstructions, which belongs to the technical field of sewage treatment and particularly relates to a method for classifying inter-matrix obstructions and extracting components of the inter-matrix obstructions in an artificial wetland sewage treatment technology. The artificial wetland obstructions are definitely and primarily classified into five substances, i.e., protein, polysaccharide, humic acid, nucleic acid and inorganic matter, and the like; various components of the wetland obstructions are extracted by using methods of adding strong alkali, heating and centrifuging in sequence; and the components of the obstructions are quantitatively measured in combination with a Coomassie brilliant blue method, a sulfuric acid-anthrone method, a diphenylamine method, an improved lowry method and a gravimetric method. According to the invention, the components of the obstructions are classified more comprehensively, good obstruction classification and quantitative description effect is realized, the component extracting method is simple and feasible, the components of the obstructions can be measured in most of routine test labs, and the cost required for measuring the components of the obstructions is low; the method is high in popularity, simple and easy to learn; and the control conditions can be easily guaranteed and the interference resistance is good.

The present invention provides a protein and gamma-polyglutamic acid simultaneous coloring counterstaining method, which is an electrophoresis staining method belonging to the field of biotechnology, and can allow the protein on gel and gamma-polyglutamic acid to be chromogenic simultaneously. The method is characterized by adopting the following steps: (1) preparing protein and gamma-polyglutamic acid electrophoresis samples and performing polyacrylamide gel electrophoresis, (2) fixing the gel after electrophoresis, firstly staining with Coomassie brilliant blue R250 and bleaching, (3) placing the gel after the first staining in a methylene blue dye liquor for re-staining and bleaching until the protein and gamma-polyglutamic acid strips are clear. Compared with the prior art, the present invention solves the problem of simultaneous staining of protein and gamma-polyglutamic acid, can determine molecular weight and purity thereof according to the strip band position and the strip band number of the stained gamma-polyglutamic acid, and is convenient and fast to operate.

The present invention provides a method for testing the general foam protein in the beer barley and the malt. The present invention adopts an optimized albumen extracting method, uses the popular and common Coomassie brilliant blue ration rationed albumen method (Bradford method) to test the change of the general foam protein in the beer barley and malt. The method is simple and quick, and has strong applicability.

The invention discloses a method for extracting and separating total proteins of mice sperms by using two-dimensional electrophoresis. The method comprises the following steps: preparing sperms, precipitating DNAs of the sperms after lysis of the sperms, preparing sperm proteins, preparing a protein extraction sample, carrying out first-dimensional isoelectric focusing electrophoresis, carrying out second-dimensional SDS-polyacrylamide gel electrophoresis, dyeing by using coomassie brilliant blue, preparing gel and scanning by using an image scanner. According to the method, lysis of the sperms is completely performed by Trizol, the proteins are extracted by chloroform, impurities are removed by isopropanol, guanidine hydrochloride and ethyl alcohol, and the proteins are purified by acetone, so that the problems of low content of the mice sperms, collection difficulty, small cell volume, a small amount of cytoplasm and a large amount of acrosomal protease are well solved; the sample prepared by the method is complete to prepare, has a large quantity of protein isolating points, and is high in repeatability and clear in chromatogram; by virtue of detection of PDQuestsoftware, the number of the protein points is greater than 1,000; therefore, the method lays the foundation for further mass spectrometry.

The invention discloses a determination method for soluble protein content in tobacco or a product thereof. The determination method comprises the following steps: weighing a tobacco sample or a sample of its product, dissolving the sample with water and carrying out ultrasonic concussion and centrifugation so as to obtain a sample solution to be detected; dissolving bovine serum albumin in water to prepare a bovine serum albumin stock solution and diluting the bovine serum albumin stock solution to obtain bovine serum albumin standard solutions with different concentrations; dissolving Coomassie brilliant blue G-250 in 90% ethanol, adding phosphoric acid with mass concentration of 850 g / L, allowing a constant volume to be obtained through addition of water and then adding Brij-35 to prepare a Coomassie brilliant blue G-250 standard solution; and combining the bovine serum albumin standard solutions respectively with the Coomassie brilliant blue G-250 standard solution and the sample solution to be detected, sequentially introducing samples of all the reagents by running the program of a continuous flow analyzer and detecting soluble protein content in the sample to be detected under the condition of wave length of 595 nm. The method can directly detect soluble protein content without artificial comparison with a standard curve and has the advantages of simple operation, a high degree of automation, convenient sample recovery and high accuracy and repeatability.

The invention discloses a method for accelerating protein precipitation through saccharose and a micro protein measuring method capable of quickly eliminating interference of a surface active agent based on the method. The method is characterized in that an organic solvent and the saccharose are added to precipitate protein in a solution, the protein and other components such as the surface active agent are effectively separated through a centrifugal method, and the protein precipitation with the other components such as the surface active agent removed is obtained. The protein content of the protein precipitation can be measured through a coomassie brilliant blue G250 method, and the protein precipitation can also be used for SDS-PAGE or HPLC detection. The method is especially suitable for carrying out protein quantification on protein medicine containing the surface active agent. The method has the advantages that the protein recovery rate is high, the detection sensitivity is high, operation steps are simple, and detection is fast.

The invention relates to a kit for the pathologic diagnosis of tumors. The kit comprises a probe for the pathologic diagnosis of the tumors, wherein the probe comprises a gold nanoparticle cluster, an antibody of a tumor specific marker and CBB (Coomassie brilliant blue) in the molar ratio of (1-10):(1-100):(1-100); the gold nanoparticle cluster and the antibody of the tumor specific marker are connected in the manner of click chemistry; and the CBB and the gold nanoparticle cluster connected with the antibody of the tumor specific marker are combined in a static manner. The kit has high accuracy on the pathologic diagnosis of the tumors. In addition, the invention also provides a method for staining tissue sections.

The invention relates to a method for detecting the content of proteins in chitosan or a chitosan salt. The method comprises the following steps: (1) preparing a Coomassie brilliant blue G-250 test solution; (2) weighing a chitosan or chitosan salt sample, and preparing a sample solution with a concentration of 5 mg / ml; (3) preparing protein standard solutions with different concentrations, addingthe Coomassie brilliant blue G-250 test solution, and drawing an absorbance-concentration curve; and (4) adding the Coomassie brilliant blue G-250 test solution to the sample solution, measuring theabsorbance of the sample solution at 595 nm, and calculating the content of proteins in the sample. A chitosan / chitosan salt system to be detected undergoes ultrasonic treatment under a condition witha hydrochloric acid concentration of below 2% for 15 min, undergoes 100 DEG C water bath treatment, and is stained with the Coomassie brilliant blue G-250 test solution with a specific concentration,and the absorbance at 595 nm is detected, so the accuracy of the protein detection value is significantly improved.

The invention discloses a determination method for the absorption and transportation amount of six components in rhizoma bletillae in a Caco-2 cell model. The method comprises the steps of: preparing a rhizoma bletillae extract solution, a standard solution serving as a reference substance and an internal standard solution; establishing a human-derived colon adenocarcinoma cell line Caco-2 cell model; preparing a cell suspension through a Caco-2 cell model; determining the content of the six components by UPLC-MS / MS; determining the total protein content according to a Coomassie brilliant blue dye liquor protein determination kit method, and calculating the cell uptake X=the total protein of a to-be-determined substance. The invention adopts UPLC-MS / MS to establish the analysis method for the 6 components in a rhizoma bletillae extract, determines the influence of the rhizoma bletillae extract to absorption and uptake of Caco-2 cells under the conditions of time, concentration, temperature, pH and P-gh inhibitors, preliminarily evaluates the in vivo absorption characteristics of the rhizoma bletillae extract, and provides scientific basis for the research and development of the oral preparation of the rhizoma bletillae extract.

The invention relates to a marine pollutant micro-plastic dyeing method, comprising the steps of mixing Coomassie brilliant blue and methylene blue in an organic solvent to form a dye solution; selecting from marine pollutants, a mixture containing micro=plastic; dyeing the mixture with the dye solution, and keeping vibrating for dyeing for a predetermined period of time. The marine pollution micro-plastic dyeing method has the advantages that Coomassie brilliant blue and methylene blue are mixed in an organic solvent to obtain the dye solution, marine pollutants are dyed via the dye solution,dyeing time is short, dyeing acts fast, and dyeing is convenient and fast and is simple to perform. Compared with traditional high-cost Nile red, Coomassie brilliant blue and methylene blue are lowerin cost and are applicable to wide researches on marine pollutant micro-plastics.

The invention relates to a detection method of lactobacillus micro integral membrane protein active fragments. According to the invention, first, 6 N-terminal deleted or C-terminal deleted mutant proteins are established; mucin marked by HRP is adopted as a marked first antibody, and western bolt (WB) and Coomassie brilliant blue staining detections are carried out; WB results and Coomassie brilliant blue staining results are compared, such that the positions of the active fragments in the lactobacillus micro integral membrane protein sequence are obtained.

The invention discloses a method of preparing near-infrared carbon quantum dots by taking Coomassie brilliant blue as a carbon source. The preparation method comprises the following steps: weighing a certain amount of turmeric extraction residue, placing the turmeric extraction residue in a reaction kettle, adding an appropriate amount of secondary water, placing the turmeric extraction residue in an electrothermal blowing dry oven and reacting, after the reaction is ended, taking out the turmeric extraction residue, naturally cooling to a room temperature, and filtering to obtain a carbon quantum dot solution for later use; preparing a Coomassie brilliant blue solution, uniformly mixing the Coomassie brilliant blue solution with carbon quantum dots, adding an appropriate amount of secondary water, transferring the mixed solution to a hydrothermal reaction kettle, placing the mixed solution in the electrothermal blowing dry oven for reacting, after the reaction is ended, taking out the mixed solution, naturally cooling to the room temperature, and filtering to obtain the near-infrared carbon quantum dots. The product obtained by the method disclosed by the invention is stable in property and good in fluorescence performance, has important application value in the fields of bioimaging, sensing, medicine delivery, photocatalysis, biological living imaging and the like, and uses the turmeric extraction residue as a raw material for realizing waste recycling without the need of a catalyst.

The invention discloses an extraction method and application of a peanut leaf soluble protein. The extraction method comprises the following steps: (1) carrying out sun-drying and pulverization on fresh peanut leaves as a raw material; (2) adding distilled water, adjusting the pH value, and carrying out extraction; (3) centrifuging extracted solution, separating to obtain supernate, and removing precipitates; (4) adjusting the pH value of the obtained supernate; (5) centrifuging the solution after the pH value is adjusted, and precipitating, so as to separate the peanut leaf soluble protein; (6) carrying out redissolution on the obtained precipitates with distilled water, and adjusting the pH value; and (7) after the redissolution is finished, carrying out vacuum freeze drying on the protein solution, so as to obtain peanut leaf soluble protein. According to the extraction method, the peanut leaf soluble protein is extracted by virtue of an alkali solution and acid precipitation method, the extraction rate of the peanut leaf protein is high and is 44.59%, and the extraction method is easy in operation and implementation. A Coomassie brilliant blue analysis and antioxidant activitydetermination result presents that the purity of the peanut leaf soluble protein is 63.83%, and the peanut leaf soluble protein has good antioxidant activity.

Disclosed is a detection method of swiftly detecting protein nitrogen in situ, after the iTAG reagent which adopts the iTAG protein-labeled technique, or the coomassie brilliant blue method dyeing reagent, or the Folin- hydroxybenzene reagent swiftly reacts on the sample to be tested and presents color, the content of the protein nitrogen in the sample can be obtained through comparing the presented color with the standard colourimetric card. The cost of the detecting method is greatly lower than that of large-scale precision instrument, and the operation is simple and swift; although the precision is not enough, the content of protein nitrogen in milk from different batches can be swiftly obtained when food enterprises purchase mass raw material such as milk in situ, according to coloring result of the sample, whether the true protein (protein nitrogen) reaches the standard or not can be directly obtained; thereby being suitable for rough detection of mass sample in situ.

The invention provides a near-infrared spectral discrimination method for mutton adulterated with duck meat and belongs to the technical field of food safety inspection. The invention provides a method for quickly discriminating mutton adulterated with duck meat. The method comprises the following steps: (1) respectively preparing samples of mutton, duck meat and adulterated mutton, and a to-be-tested sample of unknown meat; (2) preparing Coomassie brilliant blue G-250 solution; (3) respectively taking and adding the samples into Coomassie brilliant blue G-250 solution, performing pulping and then sampling near-infrared spectrums of the samples as original spectrums; (4) performing optimized selection of modeling wave bands to the original spectrums, and respectively performing multiplicative scatter correction (MSC), standardized normal variate (SNV), area normalization, Autoscale, smoothing processing, first-order derivative processing and the like to the selected modeling bands to preprocess the original spectrums; (5) establishing adulterated mutton discrimination model by adopting a support vector machine regression modeling method. The method can realize quick, accurate and stable discrimination of mutton adulterated with duck meat.

The invention discloses a method for detecting a trace of albumin in urine, and the method comprises the following steps: adding methanol to a polyvinylidene fluoride or nitric acid cellulose membrane; after adding a series of albumin standard substances and a urine sample to be detected, staining by utilizing a Coomassie brilliant blue R250 staining agent and destaining by utilizing a destaining solution; after drying the membrane, comparing and analyzing the color shade of spots which are formed on the sample to be detected and the standard substances; carrying out the initial qualitative operation by virtue of the naked eyes; shooting or scanning the membrane; preparing a standard curve through software according to the concentrations of the standard substances and the average density of the spots; and obtaining the concentration of the sample to be detected through computing according to the average density of the spots of the sample to be detected, thereby realizing the quantitative analysis. The method disclosed by the invention has the advantages of small needed sample amount, simplicity and convenience in technical operation, reliable result and low cost, does not need special reagents and instruments and can be used for realizing the quantitative detection of a trace of albumin in the urine.

The invention relates to an improved spectrophotometry method for determining proteins by using Coomassie brilliant blue. For overcoming the shortcoming of poor stability in the conventional Coomassie brilliant blue method for determining proteins, the invention provides a Coomassie brilliant blue method for determining proteins based on increasing stability and sensitivity by adding microemulsion, which employs microemulsion, to effectively improve the stability of a system and the sensitivity of the reaction system. The determination method of the invention is a simple, rapid and quantitative method for determining proteins.

A method for screening the microbial flocculant generating bacteria features that the Congo red (C32H22N6Na2O6S2) and the Coomassie brilliant blue are simultaneously added to the culture medium for selectively screening the flocculant generating bacteria from different specimens.

The invention provides an application of epalrestat in preparation of pancreatic cancer drugs. The pancreatic cancer drugs are used for inhibiting exosome secretion of pancreatic cancer cells. A pharmaceutical composition for treating pancreatic cancer contians epalrestat. The pharmaceutical composition is used for inhibiting exosome secretion of pancreatic cancer cells. The embodiment of the invention also provides a method for verifying the inhibition effect of epalrestat on exosome secretion by pancreatic cancer cells. The method comprises the following steps: extracting cell supernatant exosomes by using a low-temperature ultracentrifugation method; lysing the collected exosomes, using a BCA kit to quantify protein, and using the measured amount of the protein to reflect the amount ofthe exosomes; using a transmission electron microscope to verify a double-layer lipid membrane wrapped cup-shaped structure of the exosomes; detecting exosome protein concentration by protein polyacrylamide gel electrophoresis with Coomassie brilliant blue. The invention provides a novel application of epalrestat, namely the inhibition of exosome secretion. The epalrestat has great application potential in clinical tumor treatment.

The invention discloses a dyeing and decoloration method of two-dimensinal electrophoresis gel Coomassie brilliant blue for screening a saline-alkaline resistant plant material. The method comprises the first step of preparing a staining solution containing Coomassie brilliant blueR250, methyl alcohol, acetic acid and distilled water with a constant volume, uniformly blending the staining solution and still standing for 0.5-1.5 hours, filtering the staining solution and then storing the staining solution at a normal temperature for use; the second step of preparing a destaining solution containing ethyl alcohol, acetic acid and distilled water with a constant volume; the third step of using distilled water to wash two-dimensional SDS gel for 2-3 times, putting the two-dimensional SDS gel in a dyeing box, adding the staining solution into the dyeing box, using a preservative film to seal the dyeing box, and conducting dyeing on the two-dimensional SDS gel on a table concentrator for 2-3 hours at the normal temperature; the fourth step of conducting decoloration, wherein the decoloration comprises the procedures of pouring out the staining solution, using the distilled water to wash the gel for 2-4 times, adding the destaining solution, wherein the dosage of the destaining solution is 1-2 times that of the staining solution, conducting decoloration on the gel on the table concentrator for 2-3 hours at the normal temperature, and using the distilled water to wash the gel for 2-4 times. According to the dyeing and decoloration method, the method is further optimized on the basis of a routine technique method, procedures of pre-immobilization and the like are omitted, the speed of the dyeing and the decoloration is fast, and the effects of the dyeing and the decoloration are good.

The invention relates to a method for detecting content of protein in a fiber. A Coomassie brilliant blue approach is applied to quantitative detection on the content of protein in the fiber. Coomassie brilliant blue G-250 is red under a free state; after the G-250 is combined with an alkaline amino acid and an aromatic amino acid residue of the protein in a dilute acid solution, the color becomes blue, the maximum absorption wavelength becomes 595nm from 465nm, the protein ranges from 1 microgram to 1000 micrograms, the absorbance of a protein-pigment conjugate under the wavelength of 595nm is in direct proportion to the content of protein, and the relationship conforms to the Lambert-Beer law, so that the Coomassie brilliant blue can be used for quantitatively determining the content of protein in the fiber. The detection process comprises the following steps: preparing a protein standard solution, preparing a Coomassie brilliant blue solution, drawing a standard curve, obtaining the equation of linear regression and the linear correlation coefficient r, preparing a test product solution, determining the content of protein in a sample, and calculating and analyzing the result.