Coomassie brilliant blue staining solution and staining method

Abstract

The invention discloses a coomassie brilliant blue staining solution and a staining method. The coomassie brilliant blue staining solution comprises 0.1-10 percent by volume of acid, 1-15 percent by volume of ethyl alcohol, 10-50g/L of soluble starch and 20-1000mg/L of coomassie brilliant blue aqueous solution. The staining solution does not contain reagents harmful to human bodies and is environmental friendly. The staining method using the staining solution has the beneficial effects that due to addition of the soluble starch, the staining background of gel can be reduced, a protein band can be specifically stained, the sensitivity of staining is effectively improved, and therefore, the whole staining process of the protein band can be completed within 20 minutes by using the staining method, the steps such as gel fixation, sensitization and destaining in a conventional dyeing process are omitted, operating steps are greatly simplified, and the dyeing time is saved; the staining method has the advantages of short cycle, high sensitivity and small background interference.

Description

technical field [0001] The invention relates to a Coomassie brilliant blue staining solution and a dyeing method using the staining solution, belonging to the field of biotechnology. technical background [0002] In the 1960s, there were reports of staining protein bands after electrophoresis with Coomassie brilliant blue staining solution to observe protein components and protein separation. In the following decades, Coomassie brilliant blue staining has become one of the standard methods for observing protein bands in protein gel electrophoresis in protein science research. This staining method is included in almost all protein research manuals. Both are described. In the classic method of staining protein gels with Coomassie Brilliant Blue, Coomassie Brilliant Blue is usually dissolved in a solvent containing acetic acid, methanol, etc. The steps of staining and decolorization take 1-2 days. It can be seen that the staining process of protein gels not only has many step...

AI Technical Summary

Problems solved by technology

[0008] The present invention solves the problems in the prior art that when staining protein bands in protein gels, the Coomassie Brilliant Blue staining solution used contains reagents harmful to the human body, and the staining steps are numerous and the cycle is lengthy, and further provides An improved Coomassie Brilliant Blue dyeing solution and a dyeing method. The dyeing solution does not contain harmful reagents to the human body and is environmentally friendly. The dyeing method using the dyeing solution can complete the entire dyeing process within 20 minutes, and after dyeing Does not require decolorization treatment, has the advantages of short cycle time, high sensitivity, and low background interference

Images