159 results about "H&E Staining Method" patented technology

The invention discloses a medical self-sputum smear dyeing device and method instead of artificial operation in the tuberculosis detection technique domain, which comprises the following parts: three-dimensional right-angle coordinate mechanical console, cam, smear operation head, dyeing liquor sprayer, sampling tag box component, sample box location board, slide location rack, pusher box, creeping pump group, and display and operation button. The method comprises the following steps: finishing automatic box opening, shooting and image display; selecting the suspected part of sputum sample artificially; gathering the sample to coat on the slide; dying and drying fractionally; preparing the smear sputum sample to satisfy the microscopic requirement. The invention prevents the detecting personnel from infection and medical pollution, which improves the quality of sputum smear and shortens the sample disposal time.

The invention provides a medical tissue section staining method which comprises the following steps that: olefin is sliced into sheets, dewaxed and hydrated; sliced sheets are put into solution of picric acid, formaldehyde and glacial acetic acid after being heated, taken out and washed by running water; the sliced sheets are put into a 5 percent sodium thiosulfate solution to soak and washed by distilled water; the sliced sheets are put into an alcian blue solution to soak and washed by the running water; the sliced sheets are put into a preheated alkaline ethanol solution to soak and washed by the running water; under the lighttight condition, the sliced sheets are put into a haematoxylin working solution and washed by the running water and the distilled water; the sliced sheets are put into a saffron/ acid fuchsine working solution and washed by the distilled water; the sliced sheets are put into a phosphotungstic acid solution to soaked, then transferred to the glacial acetic acid to soak and washed by the distilled water; the sliced sheets are dehydrated by ethanol; the sliced sheets are put into an ethanol saffron solution to soak, then dehydrated by the ethanol and sealed. The medical tissue section staining method has low cost and vivid staining, shortens the time for a staining flow and also increases the application range.

The invention provides a staining method for aramid fiber. The staining method comprises the steps of preprocessing, preshaping, staining, reduction clearing, color fixing, soft processing, drying, shaping, calendaring and rolling. According to the staining method, DEET is added in to serve as a dye carrier, and the staining effect and efficiency are improved; meanwhile, substitute alkali is adopted to replace a conventional soda substance, and not only is the usage amount decreased, but also the color fixing effect is enhanced to enable the washability of the aramid fiber is further enhanced; the reduction clearing and color fixing processes are added, and therefore the staining fastness is good; green and environmental-friendly non-formaldehyde TCD-R is adopted in the color fixing process, the fiber is dried step by step after color fixing, and therefore the firmness of the fiber is greatly improved; the soft processing process is added, the color fastness and softness of fabric are improved, the roughness of the fabric is reduced, and therefore the fabric is fluffy and rich in elasticity.

The present invention relates to a staining kit and its preparation method, staining method and its application in several detection items for detecting canceration cell, red blood cell, campylobacteriosis, vaginal cleaning degree, estrogen level, garlandosus, trichomoniasis, mycosis, diplococcus gonorrhoeae and chlamydi trachomatis on a cervical smear. Said invented kit is formed from fixatino fluid, staining solution and differentiatino solution. Its staining method includes the steps of fixation, staining, differentiation and water-washing. Its film-making speed is quick, only has need oftwo main, and its detection accuracy rate is high, can be up to 98%.

The invention relates to a dyeing method of a peal, which includes the preparation of a staining agent, the pretreatment of a peal and the dyeing the peal. Firstly, pretreatment is carried out to the staining agent and the peal to lead the bonding force between the staining agent and the peal to be greatly improved and simultaneously the peal is not damaged, thus ensuring that the peal is not easy to fade in a short time and improving the luster of the peal thereby promoting the level of the peal. Furthermore, the dyeing fluid of the peal can be repeatedly used for once or twice, thus decreasing cost and reducing pollution.

The invention discloses a coomassie brilliant blue staining solution and a staining method. The coomassie brilliant blue staining solution comprises 0.1-10 percent by volume of acid, 1-15 percent by volume of ethyl alcohol, 10-50g/L of soluble starch and 20-1000mg/L of coomassie brilliant blue aqueous solution. The staining solution does not contain reagents harmful to human bodies and is environmental friendly. The staining method using the staining solution has the beneficial effects that due to addition of the soluble starch, the staining background of gel can be reduced, a protein band can be specifically stained, the sensitivity of staining is effectively improved, and therefore, the whole staining process of the protein band can be completed within 20 minutes by using the staining method, the steps such as gel fixation, sensitization and destaining in a conventional dyeing process are omitted, operating steps are greatly simplified, and the dyeing time is saved; the staining method has the advantages of short cycle, high sensitivity and small background interference.

The invention relates to a dying solution of transparent dying of timbers or bamboos and a dying method thereof, and the dying solution comprises traditional timber or bamboo special dye and accessoryingredient consisting of methanol and water, wherein each liter of the dying solution has 0.1-10g of the special dye; the water in the accessory ingredient is less than or equal to 90% in volume percent, the water in less than or equal to 70% is preferable, and the water in less than or equal to 35% is more preferable. The dying method in the invention has simple operation and needs no pretreatment on timbers or bamboos such as bleaching and the like.

The invention discloses a preparation method of a mangosteen shell natural dye, application and a staining method thereof. The preparation method of the mangosteen shell dye includes: subjecting a mangosteen shell to washing, air drying and mechanical crushing, then adding the crushed mangosteen shell into an ethanol water solution containing sodium hydroxide, performing reflux extraction at 60-90DEG C for 0.5-2h to obtain an extracted solution, then carrying out filtration and reduced pressure distillation on the extracted solution so as to obtain the concentrated mangosteen shell natural dye. For the ethanol water solution, the mass content of ethanol is 20-40%, per kilogram of the ethanol water solution contains 0.5-1.5g of sodium hydroxide, the crushed mangosteen shell and the ethanol water solution are in a mass ratio of 1:10-30, and the mass of the concentrated mangosteen shell natural dye accounts for 1/6 to 1/2 of the mass of the ethanol water solution. The extraction process of the mangosteen shell natural dye involved in the invention causes no environmental pollution. A dyed fabric has soft color and good color fastness, is safe to wear, does not have carcinogenic and teratogenic effects or cause allergic reaction. With good ecological environmental compatibility, the mangosteen shell natural dye is biodegradable.

The invention provides an eosin staining solution comprising the components of eosin, Biebrich scarlet, flame red, ethanol and water. The invention also provides a preparation method of the eosin staining solution, a HE staining solution containing the eosin staining solution and a staining method of the eosin staining solution. When the eosin staining solution provided by the invention is used for staining slices, the background is clear, the gradation is distinct, the staining effect is good, and the slices can be read easily, cannot fade easily and are convenient to store for a long time.

The present invention provides a protein and gamma-polyglutamic acid simultaneous coloring counterstaining method, which is an electrophoresis staining method belonging to the field of biotechnology, and can allow the protein on gel and gamma-polyglutamic acid to be chromogenic simultaneously. The method is characterized by adopting the following steps: (1) preparing protein and gamma-polyglutamic acid electrophoresis samples and performing polyacrylamide gel electrophoresis, (2) fixing the gel after electrophoresis, firstly staining with Coomassie brilliant blue R250 and bleaching, (3) placing the gel after the first staining in a methylene blue dye liquor for re-staining and bleaching until the protein and gamma-polyglutamic acid strips are clear. Compared with the prior art, the present invention solves the problem of simultaneous staining of protein and gamma-polyglutamic acid, can determine molecular weight and purity thereof according to the strip band position and the strip band number of the stained gamma-polyglutamic acid, and is convenient and fast to operate.

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300.

The invention discloses an immunohistochemical double-molecular-target single staining kit as well as a using method and an application thereof. Based on an expression pattern of a molecular target ina sample to be detected, a cell core type molecular target and a cytoplasm or cell membrane or plasma membrane type molecular target are selected as the to-be-detected double-molecular targets; then,specific to the specific antibody of the double-molecular targets, a kit comprising a mixing primary antibody is formed by mixing, wherein the kit can be used in an immunohistochemical staining method such as an immuno-enzyme linked immunosorbent assay or an immune colloidal gold method; the kit and an enzyme labeled secondary antibody and a chromogenic substrate matched with the kit are adopted,so that synchronous detection of two molecular targets can be completed on one slice through one immunohistochemical staining process. The method is obviously superior to the traditional immunohistochemical and immunohistochemical double staining method, and has the characteristics of low cost and high efficiency, and does not need to increase the additional operation steps.

A deep learning-based digital staining method and system are disclosed that enables the creation of digitally/virtually-stained microscopic images from label or stain-free samples based on autofluorescence images acquired using a fluorescent microscope. The system and method have particular applicability for the creation of digitally/virtually-stained whole slide images (WSIs) of unlabeled/unstained tissue samples that are analyzes by a histopathologist. The methods bypass the standard histochemical staining process, saving time and cost. This method is based on deep learning, and uses, in oneembodiment, a convolutional neural network trained using a generative adversarial network model to transform fluorescence images of an unlabeled sample into an image that is equivalent to the brightfield image of the chemically stained-version of the same sample. This label-free digital staining method eliminates cumbersome and costly histochemical staining procedures and significantly simplifiestissue preparation in pathology and histology fields.

The invention relates to a gallinaceous visfatin gene 9bp indel polymorphism detection method and application thereof. The detection method comprises the following steps of: designing a primer in the position of a gallinaceous visfatin gene 9bp indel locus; then carrying out PCR amplification; and detecting the polymorphism of the gene 9bp indel by the polyacrylamide gel electrophoresis, wherein the gene is of a DD genotype when 9bp is deleted, the gene is of an II genotype when 9bp is inserted, and the heterozygous individual at the locus is of an ID genotype. The correlation analysis combining the detection results and economic characters of chickens is used for the assistant selection and the molecular breeding of chickens. The detection method does not need be subject to the enzyme digestion, thereby saving the cost and the time; the detection is carried out by adopting the polyacrylamide gel electrophoresis, thereby overcoming the limitation of the agarose electrophoresis to the small-fragment nucleotide difference detection. The method has the advantages of high resolution, high detection sensitivity and accurate genotype judgment; and the gel has on requirements for the staining method, and the ethidium bromide (EB) staining and the silver staining are both applicable.

The invention discloses a staining method of ciliates, belongs to the technical field of biology, and provides a staining method of ciliates to solve the problems that a traditional ciliate staining method is long in staining time and fussy in staining step, and cytostome and cilium cannot be clearly displayed. A saturated ethyl alcohol mercuric chloride solution is adopted as a fixing agent to carry out pre-fixation on ciliate bodies. In addition, before the pre-fixation operation, to-be-produced ciliate bodies are attached to a to-be-dyed glass slide; a plurality of steps such as dropwise adding protein glycerinum water and collodion are omitted. The staining method disclosed by the invention is convenient to operate; the sensitivity and the staining effect of silver staining of the ciliate protein are greatly improved; and rapid, efficient and accurate display of infraciliature in morphological classification and cytological studies of the ciliates is achieved.

The invention discloses a staining process for cashmere fiber or cashmere fabric which comprises the steps of, placing the cashmere fiber or cashmere fabric into treatment chamber, lowering the pressure in the treatment chamber to be 10Pa or below, adjusting the pressure in the chamber to be 10-200Pa through non-polymer gas, exerting voltage to the electrode arranged in the processing chamber for low-temperature plasma treatment, and proceeding coloration.

The invention discloses a DNA silver staining method in polyacrylamide gel (PAGE) electrophoresis, which comprises the steps of soaking polyacrylamide gel with 0.1% AgNO3 for 10-15min, washing with distilled water twice, developing with 0.04% Na2CO3, 2mL of 37% HCOH/L and 2% mixed solution for 5-6min, and flushing twice with distilled water to complete the whole dyeing process. The conventional DNA silver staining method needs 5-6 chemical reagents, prepares 4 solutions and spends 40-60min to complete the dyeing step, but the method only needs 4 chemical reagents, prepares 2 solutions and spends 20min to reach the result the same as that of the conventional dyeing method, and in denaturing polyacrylamide electrophoresis (PAGE), the dyeing method can reduce the amount of DNA to 0.44ng; and in non-denaturing polyacrylamide electrophoresis (PAGE), the amount of DNA can be reduced to 3.5ng. Compared with the conventional dyeing method, the method of the invention saves the time, reduces the cost, and increases the detection sensitivity.

The invention discloses a staining method for glass fiber cloth and a multiple-shaft transmission device thereof. The staining method includes the following steps: the glass fiber cloth is tensioned through the multiple-shaft transmission device, and can move in a certain speed; when the glass fiber cloth moves evenly, the glass fiber cloth is painted in a reciprocating mode by using the method of paint spraying at the same time so that the glass fiber cloth can have needed colors; and after being painted, the glass fiber cloth is moved as well as dried, and is packed into a roll through the multiple-shaft transmission device. By means of the staining method for the glass fiber cloth, continuous production of glass fiber cloth staining is achieved, meanwhile, process is simplified, and the production and packing can be carried out smoothly; and the stained glass fiber cloth can be widely used in composite materials and provide people with good visual feelings and appearance performance.

This invention provides a dyeing method and its device for the dyeing liquid dyes the fabric. In this invention, the nip angle between the eject direction of the dyeing liquid and the moving direction of the fabrics is at least 90íÒ, or opposite. Comparing to the present technology, this invention can improve the filter effect of the dyeing liquid in the fabrics.

The invention discloses a tumor cell detecting method, and belongs to the technical field of tumor cytology. The detecting method comprises the following steps of applying alpha fetoprotein with enzyme labeling or alpha fetoprotein heteroplasmon to combine with alpha fetoprotein acceptor heteroplasmon expressed by tumor cell; through the chromogenic reaction between substrate and enzyme, dyeing the tumor cell, and thereby distinguishing the tumor cell and the normal cell. The tumor cell detecting method can specially identify the tumor cell; the method overcomes the shortcomings of the traditional dyeing method, and is applicable to various tissue specimens or cell specimens; therefore, the method has very abroad application prospect.