64 results about "Specific staining" patented technology

The present invention provides compounds having high affinity for amyloid β-protein which are for the diagnosis of diseases in which amyloid β-protein accumulates, for agents for specifically staining amyloid β-protein, and for the treatment and / or prophylaxis of diseases in which amyloid β-protein accumulates. The present invention also provides probes and staining agents for neurofibrillary tangles.

The invention discloses fluorochrome for cytolysosome positioning and a preparation method and application thereof. The preparation method of the fluorochrome compound includes the steps that a benzophenone derivative containing methyl substituents is reacted under the catalysis of Zn / TiC14, an obtained product and N-bromosuccinimide are subjected to a bromination reaction, then a product is reacted with morpholine, and the tetraphenyl ethylene derivative stain containing morpholine groups is obtained. The preparation method is easy to implement, raw materials are easy to get, and reaction conditions are mild. The obtained fluorochrome contains tetraphenyl ethylene fluorescence groups capable of gathering the activity of inducing fluorescence emission and can be used for performing specific staining on all kinds of cytolysosome. The fluorochrome has the advantages of being small in cytotoxicity, stable in cell metabolism resistance, good in photobleaching resisting effect and the like and hardly affects normal physiological activities of cells, and a new method is provided for observing the morphologic changes of cytolysosome for a long time. The fluorochrome can be widely applied to monitoring the morphology of cytolysosome, observing the apoptosis process and the like.

The invention discloses a method for positioning immune tissues of growth hormone for malus plants and application thereof. The method for positioning immune tissues of growth hormone for malus plants comprises the following steps of: (1) fixing plant tissues to obtain fixed plant tissues; (2) carrying out slice making on the fixed plant tissues on the basis of the step (1) to obtain a plant tissue slice; and (3) immunostaining the obtained plant tissue slice on the basis of the step (2), and determining the distribution condition of hormone in the plant tissues through observing a position of a positive signal. The method for positioning immune tissues of growth hormone for malus plants can eliminate the easily caused influence of nonspecific staining of woody plant tissues, and can identify the positive signal clearly. The method for positioning immune tissues of growth hormone for malus plants verifies that the growth hormone has tissue specificity when being distributed in the tissues of a stem tip or a root tip of an apple tree in a condition of iron deficiency.

The invention discloses a coomassie brilliant blue staining solution and a staining method. The coomassie brilliant blue staining solution comprises 0.1-10 percent by volume of acid, 1-15 percent by volume of ethyl alcohol, 10-50g / L of soluble starch and 20-1000mg / L of coomassie brilliant blue aqueous solution. The staining solution does not contain reagents harmful to human bodies and is environmental friendly. The staining method using the staining solution has the beneficial effects that due to addition of the soluble starch, the staining background of gel can be reduced, a protein band can be specifically stained, the sensitivity of staining is effectively improved, and therefore, the whole staining process of the protein band can be completed within 20 minutes by using the staining method, the steps such as gel fixation, sensitization and destaining in a conventional dyeing process are omitted, operating steps are greatly simplified, and the dyeing time is saved; the staining method has the advantages of short cycle, high sensitivity and small background interference.

The invention discloses a tumor necrosis factor-alpha induced protein 8 L3 (TIPE3) immunohistochemistry detection kit for diagnosing lung cancer. Contents of the kit comprise a reagent which is used for inactivating endogenous peroxidase and biotin in a human tissue section, a non-specific protein blocking agent which is used for blocking non-specific staining of cross protein reaction in the human tissue section, a rabbit anti-human initial antibody which is combined with human tissue antigen, an antibody diluent, a goat anti-rabbit monoclonal connection antibody which can be connected with the initial antibody and is marked by horse radish peroxidase, a developing reagent which can be reacted with the horse radish peroxidase and used for developing, and a reagent which can specifically stain nuclei in tissues. TIPE3 is positively expressed in various kinds of pathological type lung cancer, tumors can be early detected and found, the kit is high in specificity and high in sensitivity and can be applied to early diagnosis of the lung cancer, a patient can be effectively and timely treated, unnecessary medical cost is reduced, the survival quality of the patient is improved, survival time is prolonged, and the survival rate of the patient who suffers from the lung cancer is improved.

The invention relates to application of an enzyme-labeling-liquid-based cytology technology to the screening of bladder cancer. In the technology, a cell sample which is tabletted by a liquid-based tabletting method is treated by an acid phosphatase chemical staining method, and a bright red precipitate is formed in cytoplasm of abnormal cells by specific staining to ensure that the abnormal cells have remarkable marks. The invention provides a kit for the detection. The kit mainly comprises an enzyme-labeling-liquid-based cytology staining reagent, enzyme-labeling-liquid-based cytology preserving fluid and settlement buffer solution, and can provide high detection specificity and a good staining effect.

The invention relates to the technical field of cell induction culture, and particularly relates to a culture method for inducing the differentiation of an umbilical cord mesenchymal stem cell into acartilage, and a culture medium used in the method. The culture medium is obtained by at least additionally adding the following components in a basal culture medium: 7 ng / mL to 13 ng / mL of TGF (Transforming Growth Factor)-beta 3, 0.07 mumol / L to 0.13 mumol / L of dexamethasone, 40 mug / mL to 60 mug / mL of ascorbate, 0.7 w / v% to 1.3 w / v% of 1*ITS (Internal Transcribed Spacer), 30 mug / mL to 50 mug / mL of L-proline and 0.7 mmol / L to 1.3 mmol / L of sodium pyruvate. According to the culture medium, through optimizing an induction component and matching a specific culture method, the induction velocity is improved; the hard and compact cartilage can be formed after 7 days; all inoculated mesenchymal stem cells can be differentiated into cartilage cells, and the differentiation rate is improved. An induced cartilage globule is compact in texture, and displayed through specific staining, and all mesenchymal stem cells are sufficiently differentiated into the cartilage cells.

Methods and compositions for chromosome-specific staining are provided. Compositions comprise heterogeneous mixtures of labeled nucleic acid fragments having substantially complementary base sequences to unique sequence regions of the chromosomal DNA for which their associated staining reagent is specific. Methods include methods for making the chromosome-specific staining compositions of the invention, and methods for applying the staining compositions to chromosomes.

The invention belongs to the technical field of biology and particularly relates to a canine parvovirus IgG antibody detection kit. The kit is simple in operation procedure in clinical sample detection, and can complete detection in 3.5 h, result determination only need a common inverted microscope without special instruments, specific staining is visible by naked eyes, and non-specific staining is easy to differentiate, thus facilitating laboratory and clinical on-site detection. Commercial canine parvovirus ELISA antibody detection kits at present are high in cost and need high preparation techniques and production conditions, while the kit is low in material cost and simple in preparation process, and the characteristics of high stability, high sensitivity and high throughput of the kit can be compared favourably with that of the commercial canine parvovirus ELISA antibody detection kits.

The invention relates to a microbial fluorescent staining solution and an application thereof. The microbial fluorescent staining solution comprises lectin, fluorescein, nucleic acid dye, a buffer solution, an anti-quenching agent, a bacteriostatic agent and water; the microbial fluorescent staining solution can be used in microbial dyeing, specific recognition of agglutinin on glycoprotein and specific staining of nucleic acid by nucleic acid dye are utilized, according to the invention, the fungi are blue, green or red, the bacteria are red, the morphology is combined to achieve the detection on the microorganisms in a vaginal secretion sample, a cervical exfoliated cell sample, a skin sample and a sputum sample, the detection result can be visualized, and the microorganism variety can be directly and reasonably judged.

The invention provides full-spectrum high-brightness high-stability fluorescent dye. The dye is prepared by mixing one or more selected from 4-acylamino-substituted naphthalimide dye, bialkoxy-substituted naphthalimide fluorescent dye, diamino-substituted naphthalimide fluorescent dye, 9,10-diamino-substituted perylene bisimide dye, six-membered rhodamine dye, five-membered rhodamine dye, and Si-rhodamine dye according to any ratio. Compared with existing commercial dye, the fluorescent dye provided by the invention is higher in light stability, narrower in half-peak width (25 nm) and insensitive to various external environments such as pH, polarity, temperature and the like. Through introduction of Click groups, protein labels, drug molecules and other active groups, the obtained functionalized fluorescent molecules have very high biocompatibility, and can be used for rapid and specific staining of living cells and living bodies. In addition, due to the improvement of light stabilityand fluorescence brightness, the dye realizes super-resolution fluorescence imaging in various modes such as Storm, STED and SIM.

A semi quantitative detection method of constitutive conversion for sperm nucleoprotein includes adding the binding solution in semen and coating it on staining window of glass slide to make sperm stick on glass slide easily, adding the staining solution in said semen to stain semen, mounting semen and counting out percentage of sperm with blue head from certain amount of sperms. The reagent for realizing the method consists of staining solution, binding solution for sticking semen on glass slide easily and eluent for washing out nonspecific staining after semen being stained.

Application of gold-silver mixed-metal cluster compound in preparing fluorescence staining reagents for cell nucleoli relates to a fluorescence staining reagent. The invention provides an application of the gold-silver mixed-metal cluster compound which has excellent specific staining effect to cell nucleolus area quickly and stably, in preparing fluorescence staining reagents. The gold-silver mixed-metal cluster compound [Au6Ag2(C)(dppy)6](BF4)4 is synthesized using the method in the document: Jian-Hua Jia, Quan-Ming Wang; J. Am. Chem. Soc. 2009, 131, 16634-16635. The dppy is diphenyl-2-pyridylphosphine. The gold-silver mixed-metal cluster compound has fluorescence staining effect to cell nucleolus area, wherein the cell nucleolus fluorescence staining performance of the gold-silver mixed-metal cluster compound reaches or exceeds that of existing like products, so the compound can be used for preparing fluorescence staining reagents for cell nucleoli.

The invention discloses a coomassie brilliant blue staining solution and a staining method. The coomassie brilliant blue staining solution comprises 0.1-10 percent by volume of acid, 1-15 percent by volume of ethyl alcohol, 10-50g / L of soluble starch and 20-1000mg / L of coomassie brilliant blue aqueous solution. The staining solution does not contain reagents harmful to human bodies and is environmental friendly. The staining method using the staining solution has the beneficial effects that due to addition of the soluble starch, the staining background of gel can be reduced, a protein band can be specifically stained, the sensitivity of staining is effectively improved, and therefore, the whole staining process of the protein band can be completed within 20 minutes by using the staining method, the steps such as gel fixation, sensitization and destaining in a conventional dyeing process are omitted, operating steps are greatly simplified, and the dyeing time is saved; the staining method has the advantages of short cycle, high sensitivity and small background interference.

Methods of reducing nonspecific binding of a fluorophore to cells expressing a Fc receptor, for example, CD64, is provided.

The invention discloses a pathological prostate tissue large section kit. The kit comprises an embedding box, an embedding mold and a sample clamp which are arranged in a storage box. The embedding box comprises an embedding box body, a partition plate I, an elastic pressing device I, an embedding box cover and a mounting ring; a bottom plate of the embedding box body, the embedding box cover andthe partition plate I are a plate body structure with meshes, prostate tissues after pathological sampling is arranged between the partition plate I and the bottom plate of the embedding box body, andthe elastic pressing device is arranged between the partition plate I and the embedding box cover; and the embedding mold comprises an embedding mold box body, a partition plate II and an elastic pressing device II. In the invention, integrity and consistency of slide preparation can be effectively improved, a specimen loss is reduced, homogeneity of continuous stepped sections is ensured, various specific staining is carried out on the basis, and reliability of pathological clinical diagnosis evaluation and objectivity of basic research biological information diversity analysis are improvedso that the reliability of pathological detection gold standards is reflected.

The invention provides a ready-to-use antibody and a diluent thereof. An SP (streptavidin-perosidase) method is adopted, a ready-to-use antibody c-erbB-2 monoclonal antibody stock solution is selected and diluted with the diluent in the volume ratio being 1:2, 1:4, 1:8 and 1:16 for IHC (immunohisto chemistry) examination, the optimum dilution concentration of the ready-to-use c-erbB-2 working solution is 1:4, and dyeing effects of weakest background dyeing, largest-intensity specific dyeing and optimum contrast ratio are realized. Ready-to-use antibody dilution is an IHC quality control method worth popularization and use.

The invention belongs to the technical field of molecules and cellular biology, and particularly relates to a constructing method of a mouse intestinal epithelium pit cell line. The method comprises the steps that a single cell suspension of pit epithelium cells is prepared through a special separation method, cellular immortality is conducted after cell adherence. The form of intestinal epithelium primary cells is well maintained by the cell line, and high proliferation capability is achieved. Characteristic organ structures can be formed in three-dimensional culturing conditions, it is indicated by specific staining that cells which form the structure comprise multiple intestinal epithelium differentiated progeny. Pit cells of an established line can only grow in primary culturing conditions still, and the pit cells can be applied to the establishing of a mouse intestinal epithelium in vitro multi-dimensional study model which is used for studying intestinal physiologic functions andpathological mechanisms including infection, carcinogenesis and the like, and tools and platforms are provided for treatment targets and medicine screening and evaluating of intestinal diseases including intestinal cancer.

The invention provides a breast cancer cell dyeing method. The method takes anti-pan cytokeratin antibody (AE1 / AE3) as the primary antibody, and adopts a horseradish peroxidase labeled rabbit antimouse antibody as the second antibody to label breast cancer cells, by means of diaminobenzidine (DAB) and hematoxylin dyeing, breast cancer cells can be dyed specifically. The invention also puts forward application of the dyeing method and a kit for carrying out the method. The method provided by the invention has the advantages of simple operation and good accuracy, can be used for detection of breast cancer micrometastatic cells in bone marrow blood and peripheral blood samples of breast cancer patients, and has important reference significance to prognosis of breast cancer.

The invention discloses an anti-human B7-H1 (PD-L1) monoclonal antibody, an application of the antibody to immunohistochemical detection and an antibody heavy chain and light chain variable region identification method, and relates to an anti-human B7-H1 monoclonal antibody preparation method and an application of the monoclonal antibody to immunohistochemical sample detection and tumor tissue B7-H1 analysis. A heavy chain variable region (mVH) and a light chain variable region (mVL) are extracted from hybridoma cells secreting the anti-human B7-H1 monoclonal antibody, and B7-H1 heavy and light chain variable sequences are verified by sequencing. A eukaryotic cell strain expression antibody is implemented on the basis, the obtained antibody has good binding capacity according to verification, specific staining for tumor specimens is performed by immunologic tissue chemical detection, and the staining effects and specificity of the self-developed B7-H1 monoclonal antibody and a commercial B7-H1 antibody for the same tissue specimen are compared.

The present invention relates to an apparatus (10) for determining cellular composition in one or more tissue sample microscopic images. It is described to provide (210) first image data of at least one tissue sample. The first image data relates to a non-specific staining of a tissue sample of the at least one tissue sample. Second image data of the at least one tissue sample is provided (220). The second image data relates to specific staining of a tissue sample of the at least one tissue sample. Either (i) the tissue sample that has undergone specific staining is the same as the tissue sample that has undergone non-specific staining, or (ii) the tissue sample that has undergone specific staining is different to the tissue sample that has undergone non-specific staining. A non-specific cellular composition cell density map is determined (230) on the basis of the first image data. A specific cellular composition cell density map is determined (240) on the basis of the second image data. Information regarding cellular composition in the at least one tissue sample is determined (250) on the basis of the non-specific cell density map and the specific cell density map.

The invention discloses a method for positioning immune tissues of growth hormone for malus plants and application thereof. The method for positioning immune tissues of growth hormone for malus plants comprises the following steps of: (1) fixing plant tissues to obtain fixed plant tissues; (2) carrying out slice making on the fixed plant tissues on the basis of the step (1) to obtain a plant tissue slice; and (3) immunostaining the obtained plant tissue slice on the basis of the step (2), and determining the distribution condition of hormone in the plant tissues through observing a position of a positive signal. The method for positioning immune tissues of growth hormone for malus plants can eliminate the easily caused influence of nonspecific staining of woody plant tissues, and can identify the positive signal clearly. The method for positioning immune tissues of growth hormone for malus plants verifies that the growth hormone has tissue specificity when being distributed in the tissues of a stem tip or a root tip of an apple tree in a condition of iron deficiency.

The present invention relates to an apparatus (10) for determining cellular composition in one or more tissue sample microscopic images. It is described to provide (210) first image data of at least one tissue sample. The first image data relates to a non-specific staining of a tissue sample of the at least one tissue sample. Second image data of the at least one tissue sample is provided (220). The second image data relates to specific staining of a tissue sample of the at least one tissue sample. Either (i) the tissue sample that has undergone specific staining is the same as the tissue sample that has undergone non-specific staining, or (ii) the tissue sample that has undergone specific staining is different to the tissue sample that has undergone non-specific staining. A non-specific cellular composition cell density map is determined (230) on the basis of the first image data. A specific cellular composition cell density map is determined (240) on the basis of the second image data. Information regarding cellular composition in the at least one tissue sample is determined (250) on the basis of the non-specific cell density map and the specific cell density map.

The present invention concerns detection of specific tissue objects within thin sections of tissue samples as imaged in a brightfield microscope, such as a predetermined type of immune cells, without using a chromogenic stain that is specific to those tissue objects. The invention uses fluorescent stain and fluorescence imaging to detect these tissue objects. By combining a brightfield and a fluorescence image of the same tissue sample, it is possible to automatically identify objects in the brightfield image that have been stained for in the fluorescence image. The fluorescence stain does not affect the appearance of the tissue sample under the brightfield microscope. Therefore, the invention is ideal to automate the collection of training data for machine learning systems that are to be trained to detect these specific tissue objects in brightfield images of tissue that has not been stained to specifically highlight these tissue objects.

The present invention provides a new anti-LSP1 (Leukocyte specific protein 1) antibody. This new antibody allows the specific staining of inflammatory dendritic cells and can be used in diagnosis methods or as a medicament when conjugated to a drug.

The invention discloses a next generation sequencing platform-based noninvasive target mitochondrion sequencing method. The method comprises the following steps of preparing a sample slice and performing staining; capturing mitochondria through laser microdissection, wherein the number of mitochondrion tissues captured through the laser microdissection is 1-200; performing DNA extraction; performing PCR amplification and product detection; performing library construction and quality inspection; and performing computer sequencing and result analysis. Through a specific staining technology, the mitochondrion tissues are stained; by utilizing a laser microdissection technology, mitochondrial DNAs are obtained in a targeted way; through micro DNA extraction and amplification technologies, the mitochondrial DNAs are obtained and used for downstream library construction and detection; and the experimental design is rigorous, the experimental process is simple, a detection result is accurate and reliable, the scientific research and clinical detection demands can be met to varying degrees, the method is suitable for quick detection of mitochondrion-related mutation in scientific research and clinic, and correct diagnosis and classification of clinical diseases are facilitated.

The invention discloses a rhodamine dye containing a water-soluble substituent and a functional derivative (a zinc ion probe and an antibody labeled dye) of the rhodamine dye. The rhodamine dye has a structure as shown in a general formula I. After the N-site of a parent nucleus of the dye is connected with different water-soluble substituents, the biocompatibility of the parent nucleus of the dye is greatly improved; and specifically, non-specific staining in cells is remarkably reduced, cell apoptosis signals do not appear any more, phototoxicity is remarkably reduced (the zinc ion probe), labeling efficiency is improved, observation time is prolonged (the antibody labeled dye) and the like. The fluorescent dye disclosed by the invention can be applied to the fields of observing insulin release of in-vitro live pancreas islets, immunofluorescence labeling and the like.