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Ready-to-use antibody and application thereof

An antibody, general-purpose technology, used in measurement devices, instruments, scientific instruments, etc., can solve problems such as false positives, background coloration of tissue sections, etc.

Inactive Publication Date: 2015-11-18
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the operation, we found that the c-erbB-2 ready-to-use antibody caused background staining or false positives in tissue sections, and false positive reactions caused by improper antibody dilution

Method used

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  • Ready-to-use antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A total of 60 breast cancer resection specimens were randomly selected from the operating room of Renhe Hospital of China Three Gorges University. Each specimen was standardized and fixed, dehydrated, transparent, dipped in wax and embedded.

[0019] Reagent

[0020] The ready-to-use rabbit anti-human c-erbB-2 monoclonal antibody (Catalog#JY-0044) and antibody diluent were purchased from Thermo Company, and the chromogenic system was purchased from DAKO Company.

[0021] instrument

[0022] Water-proof electric heating constant temperature incubator (HENGZI, Shanghai Yuejin).

[0023] Dyeing step

[0024] IHC staining adopts SP method. All reagents were prepared and used during the experiment. Four gradient ratios of 1:2, 1:4, 1:8 and 1:16 were used for the re-diluted primary antibody. The primary antibody or ready-to-use primary antibody described herein refers to the ready-to-use antibody c-erbB-2 or the mixture of the ready-to-use antibody c-erbB-2 and antibody...

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Abstract

The invention provides a ready-to-use antibody and a diluent thereof. An SP (streptavidin-perosidase) method is adopted, a ready-to-use antibody c-erbB-2 monoclonal antibody stock solution is selected and diluted with the diluent in the volume ratio being 1:2, 1:4, 1:8 and 1:16 for IHC (immunohisto chemistry) examination, the optimum dilution concentration of the ready-to-use c-erbB-2 working solution is 1:4, and dyeing effects of weakest background dyeing, largest-intensity specific dyeing and optimum contrast ratio are realized. Ready-to-use antibody dilution is an IHC quality control method worth popularization and use.

Description

technical field [0001] The invention provides a ready-to-use antibody c-erbB-2 and its dilution, as well as an application in quality control in immunohistochemical staining. Background technique [0002] Immunohistochemical technology (ImmunohistoChemistry, IHC) applies the basic principle of the specific combination of antigen and antibody, and through chemical reaction, the chromogenic agent (such as isotope, fluorescein, metal ion and enzyme, etc.) of the labeled antibody can be used to locate, qualitatively and quantitatively display the tissue The process of intracellular antigens. IHC is currently the most commonly used auxiliary detection method for diagnosis of diseases and difficult diagnosis cases in pathology departments, and is the main reference index for personalized treatment of tumors, prognosis judgment and drug selection. Doing a good job in the quality control of IHC is the foundation to guide the diagnosis and treatment of diseases. [0003] Many facto...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/574
CPCG01N33/577G01N33/57415
Inventor 王晓星易慕华方捷迪张梦玲
Owner CHINA THREE GORGES UNIV
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