The invention discloses a liquid-phase chip for the early diagnosis of myocardial infarction, which mainly comprises coated microspheres, wherein, the coated microspheres comprise the microspheres which are coated by CK-MB capture antibodies, the microspheres which are coated by Mb capture antibodies, the microspheres which are coated by cTnI capture antibodies, the microspheres which are coated by GPBB capture antibodies, the microspheres which are coated by H-FABP capture bodies, detection antibodies which are respectively labeled by biotin, and a streptavidin phycoerythrin. The liquid-phase chip for the early diagnosis of the myocardial infarction which is provided by the invention has the advantages of high detection efficiency, a small number of the needed samples, strong specificity, high sensitivity, etc. At the same time, all the myocardial damage markers can be freely combined, thus the usage is convenient. Moreover, all the reactions are in the liquid-phase environment, which is better for keeping the natural conformation of the protein, and leads the reaction of a probe and an object to be detected to be faster and more complete, thus greatly improving the detection sensitivity and the linear range.