The invention relates to a method for preparing
protein-based surfactant by opening
protein disulfide linkage. The method is characterized by converting
disulfide linkage into sulfonic group by oxidizing
disulfide bond in
protein of
peroxide, and opening the folded coiling structure of the protein to represent the surface activity properties so as to prepare the protein-based surfactant which has the functions of emulsification, foaming, defoaming, washing, decontamination, soakage, and the like; by introducing polar group or nonpolar group into a protein
side chain with opened
disulfide bond, the surface activity properties of
peptide fragments can be further improved or increased; moreover, by adopting treatments including acetylization,
succinylation,
acylation or
phosphorylation,
protein surface activity properties such as
surface tension, emulsification, foaming, defoaming, washing, decontamination and soakage can be changed further. The method furthest prevents protein polar group and nonpolar group from being damaged, and maintains the
structural integrity and property naturalness of
protein molecules, thereby converting the protein into natural high-performance surfactant.