30 results about "Minimum free energy" patented technology

The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.

A method and device for locating an emitting source of which measurements of emission propagation at locations different from that of the emitting source can be obtained from those locations, lacking space perception, using a sensor mobile along a self-generated path. After having obtained an emission propagation measurement from the mobile sensor at the mobile sensor location, a free energy variation for moving the sensor from its current location to each of plural possible next locations of the mobile sensor is computed, the free energy being computed as a function of a standardized projected probability field of the location of the diffusing source based on previous emission propagation measurements obtained along the self-generated path. A minimum free energy variation value amongst the computed free energy variations is determined and the location associated with the determined minimum free energy variation is identified as being the next location of the sensor.

The invention discloses an acquiring method for a regular second-order structure surface liquid drop contact angle. The method comprises steps of deduction of a fundamental assumption of any three-dimensional structural model thermodynamic analysis and a system relative free energy function, sweeping from the top to the bottom end of a second-order structure to elicit relative free energy function of a system under different segments according to differences of an infiltration model and infiltration depth of a liquid drop along a depth direction of the second-order structure, programming a second-order structure volume geometric constraint condition and the relative free energy function via Matlab software, seeking relative free energy of the system under different infiltration models and infiltration depths in a segmental way, and determining a system minimum free energy and a stable wetting state to achieve a relative balance contact angle. The acquiring method solves deficiencies of redundant test measuring process of solid surface contact angle and great result error in the practical application.

The invention discloses a solid propellant formulation energy optimization design method based on a genetic algorithm. The method comprises a first step of modeling, and building an energy characteristic calculation model of solid propellant according to a minimum free energy principle; a second step of setting and storing initial parameters, inputting component species used by the solid propellant, and a chemical formula and a quality proportion range of each component, and inputting species of combustion products produced by the solid propellant after being combusted, and chemical formulas and relative molecular mass of all the combustion products or selecting all the combustion products in a combustion product data base; and a third step of calling a genetic algorithm module by a data processor, and conducting optimization design to quality proportion of the designed solid propellant. The solid propellant formulation energy optimization design method based on the genetic algorithm is simple in steps, reasonable in design, convenient to achieve, good in using effect, capable of fast obtaining an optimum proportion of highest specific impulse of the solid propellant and effectively overcoming the defects, existing in an existing solid propellant compound design process, of being high in energy characteristic test cost, long in period, large in test dose, and the like.

The invention relates to a ployglutamine pathogenesis analyzing method which includes the following steps of S1, selecting a protein model which is a two-dimensional integral HP model; S2, determining an energy function; S3, searching for a protein two-dimensional structure with the minimum free energy, namely, searching for a two-dimensional structure corresponding to protein with the minimum energy by the adoption of MPI parallel algorithm of a peer-to-peer mode. Compared with the prior art, individual temperatures of the structure are determined through the sequence of individuals in a polyglutamine protein structure group, higher temperature is distributed to the inferior individuals so that the inferior individuals are more likely to change into the superior individuals, and meanwhile, by means of cooling factors, convergence speed is increased in a parallel mode.

Provided is a reliable semiconductor device with a layered interconnect structure that may develop no trouble of voids and interconnect breakdowns, in which the layered interconnect structure comprises a conductor film and a neighboring film as so layered on a semiconductor substrate that the neighboring film is contacted with the conductor film. In the device, the materials for the conductor film and the neighboring film are so selected that the difference between the short side, ap, of the rectangular unit cells that constitute the plane with minimum free energy of the conductor film and the short side, an, of the rectangular unit cells that constitute the plane with minimum free energy of the neighboring film, {|ap−an| / ap}×100=A (%) and the difference between the long side, bp, of the rectangular unit cells that constitute the plane with minimum free energy of the conductor film and the long side, bn, of the rectangular unit cells that constitute the plane with minimum free energy of the neighboring film, {|bp−bn| / bp}×100=B (%) satisfy an inequality of {A+B×(ap / bp)}<13. In this, the diffusion of the conductor film is retarded.

The present invention provides a kind of prediction method and device of the ribonucleic acid (RNA) pseudoknot structure based on k stem, and prediction method comprises the following steps: input a section of ribonucleic acid base sequence; Define pseudoknot, k (k≥1) stem; From Search RNA bases and k stems from left to right, and mark all k stems found out; according to the characteristics of k stems crossing to form pseudoknots, find pseudoknots; calculate the minimum structure of ribonucleic acid pseudoknots containing k stems Free energy; pseudoknot structure for export of ribonucleic acid. The method involved in the present invention has fast search speed, high correct rate, sensitivity and specificity, and is superior to other related algorithms, such as PKNOTS algorithm. This method is more effective than the PKNOTS algorithm in the prediction of planar pseudoknots.