12results about How to "The method is simple and fast" patented technology

The application discloses a village feature recognition method and device based on multi-source data, and belongs to the technical field of image recognition and artificial intelligence. The method comprises the following steps: generating a first population outflow characteristic value of a target village based on population census data; generating a second population outflow characteristic value of the target village based on a night light remote sensing image of the target village; generating a third population outflow characteristic value of the target village based on electricity consumption data; calculating a population outflow comprehensive characteristic value by adopting a multi-target linear weighting function method based on the first population outflow characteristic value, the second population outflow characteristic value and the third population outflow characteristic value; and identifying the hollowing degree of the village based on the population outflow comprehensive characteristic value. The application improves the accuracy of the hollowing degree recognition of the village, and the method is simple and fast.

The application discloses a kind of pin type coupler gap detection device and method under analog hitching state, its device includes: hitching hook head, hitching hook head side is equipped with with the hook tongue cavity of the pin type coupler to be detected and with the arc structure of hook tongue cavity adaptation hitching groove;Fixed support frame, with the car coupler body of the pin type coupler to be detected fixed connection and hitching hook head and fixed support frame between sliding connection;Displacement detection device and hitching force simulation device are respectively used to measure the relative displacement amount between two and simulate the interaction between two.The method can detect the gap of pin type coupler or provide guidance for the processing of the tongue of pin type coupler by the above equipment.Compared with prior art, the beneficial effects of the present application are: by being equipped with quantitative visual indication mechanism, can be used with single set of car coupler hook head, i.e.real-time measurement of car coupler hitching gap greatly improves the accuracy and convenience of measuring pin type coupler hitching gap.

The invention discloses a high-flux toxicity pre-evaluation substitution method based on an acetylcholin esterase target. By integrating an SH-SY5Y nerve cell endogenous AChE and recombinant exogenous AChE dual detection system and relying on an automatic screening system constructed by integrating core equipment such as an automatic pipetting workstation, a constant-temperature incubator and a multifunctional microplate reader, a high-flux toxicity pre-evaluation substitution method based on an AChE target is successfully established and is used for evaluating the neurotoxicity of a compound, and the method has the advantages of high sensitivity, high sensitivity, high sensitivity and the like. The method solves the limitation that a single screening method in the prior art cannot evaluate factors such as target inhibition, cell permeability and metabolic transformation at the same time, and the method is simple, convenient, rapid, high in accuracy and suitable for large-scale primary screening of the AChE inhibitor and early warning of neurotoxicity risks.

This invention belongs to the field of immobilized enzyme technology, specifically relating to a method for constructing and applying an immobilized enzyme system based on an artificial antibody-antigen. The method includes the following steps: (1) using Fe3O4 as a magnetic core and modifying it with tetraethyl orthosilicate; (2) grafting double bonds onto its surface using MPS; (3) constructing an artificial antibody material using catechol as a template molecule; (4) preparing a catechol-enzyme complex by reacting the enzyme molecule with catechol via a Schiff base reaction; and (5) adding the catechol-enzyme complex solution to the artificial antibody material to immobilize the enzyme. This invention provides a simple and convenient method that achieves specific and directional immobilization of the enzyme while reducing costs, improving enzyme stability, and exhibiting a high immobilization rate.

The invention belongs to a rapid drawing method, and particularly relates to a rapid drawing method for two extrapolation points, perpendicular to a straight line, of a sandstone type uranium mine plane graph. The method comprises the following steps: S1, setting a ten-digit code of a working area; s2, determining an arbitrary engineering point and an arbitrary engineering straight line in the working area plan; S3, determining an extrapolation distance mode in which the arbitrary engineering point is vertical to the arbitrary engineering straight line in the working area plan; s4, calculating; s5, solving an azimuth angle; and S6, solving an extrapolation point. The method has the advantages that the method is suitable for rapidly drawing the two extrapolation points, perpendicular to the straight line, of all kinds of sandstone type uranium mine plane graphs, and is particularly suitable for compiling, storing and managing electronic and digital ore body horizontal projection graphs, the collected data is reliable in precision, labor intensity is relieved, and the method is simple, rapid, economical and feasible.

The application discloses a chicken TRAF7 gene promoter region SNP marker and application thereof in chicken breeding and belongs to the technical field of molecular genetics. The application finds that a SNP marker exists in the chicken TRAF7 gene promoter region through screening of SNPs between breeds, the nucleotide sequence of the SNP marker is shown as SEQ ID No. 1, and the 122th base from the 5' end of the sequence shown as SEQ ID No. 1 is a SNP site, the base of which is A or T. The SNPs genotype of Jining hundred-day chicken population is firstly correlated with the egg laying trait, and it is found that the individual with the TT ‑896 genotype corresponds to an earlier age of first egg laying; the individual with the TT ‑896 genotype corresponds to a higher maximum number of continuous egg laying, therefore, the molecular marker associated with the SNP site and the egg laying trait is detected, which is not only simple, fast and convenient, but also helps to breed a high-egg-yielding chicken breed and provides favorable help for breeding work.

The application belongs to the technical field of soybean molecular genetics and breeding, and discloses a KASP marker for detecting a SNP site related to a soybean petiole angle and application thereof. The SNP site is located at position 4589285 on chromosome 10 of a soybean reference genome Glycine max Wm82.a4.v1, and a C / T base substitution occurs at the site. Three KASP primers are developed according to the SNP site, and the primers are shown as SEQ ID NO. 2-4, respectively. The primers are used for PCR amplification and genotyping of a soybean to be tested, and if the detection result shows that the genotyping of the marker position is 1 / 1, it is determined that the petiole angle of the soybean variety is large; if the detection result is 0 / 0, it is determined that the petiole angle is small. The SNP molecular marker can be used for molecular marker assisted selection of the petiole angle trait of soybean, and has important theoretical and practical guiding significance for accelerating genetic improvement of compact plant type of soybean and improving breeding selection efficiency. The marker can quickly identify the petiole angle of soybean, the method is simple and fast, the identification result is accurate, and the marker has a good application prospect.

The invention discloses a method for treating sewage generated by a cage rough washing process, and belongs to the technical field of industrial sewage treatment. Sewage generated in a cage rough washing process is subjected to first-time filter pressing after being treated by polyacrylamide and polyaluminum chloride, then a flocculating agent and water are mixed and put into a liquid medicine cylinder, the agent and the sewage in the liquid medicine cylinder are synchronously conveyed into a stirring cylinder for first-time stirring, then an oil removing agent is added, and second-time stirring is continued; and carrying out secondary filter pressing on the treated water, feeding the water into a precipitation cylinder, finally adding an oxidizing agent, and standing to obtain the treated water. According to the method, by adding polyacrylamide, polyaluminum chloride, a flocculating agent and a deoiling agent, metal ions, oily components and other impurities in sewage generated in the rough washing process are rapidly removed, the impurity content and the COD value in the sewage are reduced, the treated water can be applied to the rough washing process again, and the utilization rate of water resources is increased.

The application discloses a method for removing pyrrolizidine alkaloids in plant extracts, which is characterized by comprising the following steps: S1. dissolving the plant extract with water, and then performing solid-liquid separation to obtain a clear liquid; S2. adjusting the pH of the clear liquid to 8.0-9.0, and then taking supernatant after centrifugation; then performing extraction with an organic solvent, and retaining the water phase, namely the water layer liquid; S3. performing vacuum concentration on the water layer liquid, and then performing ion exchange resin elution on the water layer liquid, and collecting the eluate; the elution operation is to first wash with water until the effluent is clear, and then perform elution with acidic ethanol and collection; and S4. performing vacuum concentration and spray drying on the eluate. The removal method significantly improves the total saponin content in some plant extracts to more than 41%, and the removal efficiency of PAs reaches more than 99%. The method can also protect the structure of bioactive substances, and improves environmental protection and economy.

This invention discloses a method related to chicken egg production. GTF2A1 This invention relates to molecular markers of SNPs in intron regions of genes and their applications, belonging to the field of molecular genetics. Specifically, it focuses on SNPs on chicken chromosome 5. GTF2A1 Four SNP loci, 5_41037360, 5_41038069, 5_41044660, and 5_41044663, were detected in the 4th, 7th, and 8th intron regions of the gene. Detecting these four molecular markers associated with egg production is not only a simple and quick method, but also helps in the breeding of early-maturing, high-producing egg-laying chicken breeds, providing valuable assistance for breeding work.

The application belongs to the field of surface treatment of metal materials, and particularly discloses a method for preparing a super-hydrophobic aluminum oxide coating on a magnesium alloy surface. The method comprises the following steps: placing the magnesium alloy into an aluminum chloride solution, heating in a water bath, and then blowing dry under an argon atmosphere for standby; combining high-temperature heating to cause a dehydration reaction; and finally using a polydimethylsiloxane (PDMS) vapor deposition surface modification method to prepare the super-hydrophobic aluminum oxide coating. The coating surface has a static contact angle of 163.6° with respect to water, and a rolling angle of 3.5°. The coating has mechanical chemical stability and corrosion resistance. The method is simple and convenient, low in cost, and has a large-scale application prospect.

The application relates to the research field of artificial intelligence assisted nanoparticle property prediction and design, and discloses a nanoparticle reverse design method based on Quasi-SMILES and a recurrent neural network, which designs a generation module and a prediction module: the generation module mainly utilizes a recurrent neural network to learn structure information of the nanoparticle, a nuclear species, a shape, a particle size, a ligand and a ligand number, and automatically generates a brand-new nano structure; the prediction module predicts numerical values of nanoparticle hydrophilicity and cell uptake through a model constructed by a classical machine learning algorithm; finally, researchers can select nanoparticles with required properties for synthesis and experimental verification according to the predicted values. Through the method, researchers can reasonably design nanoparticles with required properties and biological effects according to the model constructed by the researchers without constructing a huge compound library with prior knowledge.