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94 results about "Environmental DNA" patented technology
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Environmental DNA or eDNA is DNA that is collected from a variety of environmental samples such as soil, seawater, snow or even air rather than directly sampled from an individual organism. As various organisms interact with the environment, DNA is expelled and accumulates in their surroundings. Example sources of eDNA include, but are not limited to, feces, mucus, gametes, shed skin, carcasses and hair. Such samples can be analyzed by high-throughput DNA sequencing methods, known as metagenomics, metabarcoding, and single-species detection, for rapid measurement and monitoring of biodiversity. In order to better differentiate between organisms within a sample, DNA metabarcoding is used in which the sample is analyzed and uses previously studied DNA libraries to determine what organisms are present (e.g. BLAST). The analysis of eDNA has great potential, not only for monitoring common species, but to genetically detect and identify other extant species that could influence conservation efforts. This method allows for biomonitoring without requiring collection of the living organism, creating the ability to study organisms that are invasive, elusive, or endangered without introducing anthropogenic stress on the organism. Access to this genetic information makes a critical contribution to the understanding of population size, species distribution, and population dynamics for species not well documented. The integrity of eDNA samples is dependent upon its preservation within the environment. Soil, permafrost, freshwater and seawater are well-studied macro environments from which eDNA samples have been extracted, each of which include many more conditioned subenvironments. Because of its versatility, eDNA is applied in many subenvironments such as freshwater sampling, seawater sampling, terrestrial soil sampling (tundra permafrost), aquatic soil sampling (river, lake, pond, and ocean sediment), or other environments where normal sampling procedures can become problematic.
Enhancement and release seedling resource class evaluation method based on environmental DNA polymerization analysis
PendingCN121331216AData processing applicationsMicrobiological testing/measurementDNA extractionHigh throughput sequence
The invention discloses a method for evaluating enhancement and release seedling resources based on environmental DNA polymerization analysis. The method comprises the following steps: carrying out gridding partition on a target water area, collecting a water sample through a designed sampling scheme, and carrying out DNA extraction and high-throughput sequencing to obtain species sequence information of each sampling point. Sequencing data is subjected to species identification by using a bioinformatics method, released species are identified, a spatial abundance model is established, and a preliminary distribution map is generated. And establishing a DNA degradation kinetic model in combination with water area environmental parameters, and carrying out reverse correction on abundance distribution. And through a resource inversion model coupled with hydrodynamics, analyzing biomass distribution characteristics and migration laws of the release group, and obtaining a resource evaluation result. And finally, a species environment preference model is constructed based on migration path analysis, an optimal release area is matched in a target water area, a scientific scheme including release point locations, opportunities and quantity is generated, and a whole-process technical support and a decision basis are provided for enhancement and release.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
Real-time certification and lifecycle automation of environmental credits
A system for real-time certification and lifecycle automation of environmental credits. The system comprises: (i) a physical data collection layer equipped with on-land sensors, aerial LiDAR, satellite imagery, environmental DNA (eDNA) sampling, and community-contributed field data; (ii) an Ecological Oracle Engine, which uses an AI protocol to validate, classify, and interpret multi-source ecological data, supported by an adaptive reference library; and (iii) a smart contract protocol deployed on a blockchain network, configured to autonomously convert verified ecological data into financial instruments by issuing, pricing, transferring, freezing, and retiring tokenized environmental credits. The system performs continuous verification of each credit and includes freezing functionality based on ecological performance. It supports multiple credit types, including but not limited to carbon, biodiversity, and water credits. The system may be integrated with external modules for integrates with a Digital Twin of Nature and Web3 ecosystems, including decentralized marketplaces, ReFi platforms, and DAO mechanisms.
Owner:SYTNYK OLHA
Method for detecting apple snails
PendingJP2025151531ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyPomacea diffusa
To detect apple snails from environmental DNA with a high detection rate.SOLUTION: This method amplifies and detects environmental DNA in a sample with a first primer having a specific base sequence, a second primer having another specific base sequence, and a probe having still another base sequence.SELECTED DRAWING: None
Owner:NAT AGRI & FOOD RES ORG
Primer and method for quantitatively monitoring biomass of sargassum hemiphyllum based on environmental DNA (Deoxyribose Nucleic Acid) technology
ActiveCN121737340AMicrobiological testing/measurementDNA/RNA fragmentationSargassum hemiphyllumDeoxyribose
The invention discloses a primer and a method for quantitatively monitoring the biomass of sargassum hemiphyllum based on an environmental DNA technology, and belongs to the technical field of molecular ecology. The primer probe group comprises an upstream primer, a downstream primer and a fluorescent probe which are specifically targeted to the sargassum hemiphyllum mitochondria COX1 gene, and the sequence is shown as SEQ ID NO.1-3. The kit comprises the primer probe group, a qPCR (quantitative polymerase chain reaction) premixed solution, nuclease-free water and a sargassum hemiphyllum plasmid positive control. The method comprises the following steps: collecting a water sample, enriching eDNA, performing qPCR detection by using the primer probe group after extraction and purification, and realizing qualitative detection and quantitative evaluation of sargassum hemiphyllum through a Cq value or a standard curve. The method disclosed by the invention has the advantages of high sensitivity, strong specificity, no damage to the environment and target organisms, capability of realizing large-scale rapid general survey and the like, and is suitable for early warning of gulfweed blooms, investigation of population distribution and evaluation of ecological influence.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI
Minimum population wild plant identification and early warning method and system
The invention discloses a minimum population wild plant identification and early warning method and system. The system comprises: an environmental DNA monitoring network unit, which analyzes environmental DNA through automatic sampling, evaluates the state of species and triggers a first early warning; the biological voiceprint monitoring and recognition unit is used for monitoring related acoustic activities and illegal intrusion behaviors of the target species by using intelligent recording nodes deployed in a gridding manner and a voiceprint recognition model, and triggering second early warning; and the multi-source information fusion and causal inference unit is used for integrating the environment DNA, the voiceprint and the meteorological data, analyzing the causal relationship among the ecological factors based on a causal inference model, generating protection measures and outputting comprehensive early warning. According to the invention, all-weather, interference-free and precise monitoring of the extremely small population wild plants is realized, the problems of low efficiency, many blind areas, large interference and the like of a traditional monitoring mode are solved, and effective technical support is provided for protection of rare plants.
Owner:陕西省林业科学院
A comprehensive evaluation method and system for multi-source data management and correction of fish resources in watersheds.
This application discloses a comprehensive assessment method and system for multi-source data management and correction of watershed fish resources. The method includes: extracting multi-dimensional features from a first data matrix to obtain a first feature matrix; classifying species data based on anomaly scores, outputting verification results, and correcting the first feature matrix to obtain a second feature matrix; acquiring environmental DNA sequencing data and corresponding environmental data; obtaining a first eDNA dataset from the environmental DNA sequencing data through ecological screening; correcting the species in the first eDNA dataset to obtain a second eDNA dataset; fusing the second eDNA dataset with the second feature matrix; calculating biodiversity indices and analyzing community structure; analyzing long-term trends in population and community structure; and generating a comprehensive assessment report. This application achieves full-process automation and intelligence from data quality control and fusion to assessment, significantly improving the accuracy and efficiency of watershed fish resource assessment.
Owner:WUHAN UNIV
An environmental DNA-based primer probe combination for oncorhynchus masonii and application thereof
The application discloses a primer probe combination for Salmo platycephalus based on environmental DNA and application thereof, and belongs to the technical field of molecular biology and endangered aquatic organism monitoring. The application provides the primer probe combination for Salmo platycephalus, which comprises upstream and downstream primers and a probe P, and the amplified fragment has a length of 179 bp; the primer probe combination for Salmo platycephalus can specifically detect Salmo platycephalus in environmental DNA, can efficiently monitor and protect Salmo platycephalus, and can effectively avoid the interference of related species including Salmo, Oncorhynchus and Leucichthys bampsii; the provided primer probe combination for Salmo platycephalus can be applied to the monitoring of Salmo platycephalus in different water bodies to be detected such as streams, rivers and reservoirs, is easy to popularize, and has important significance for the protection and monitoring of Salmo platycephalus.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Method and system for comprehensive water quality assessment by integrating biotic and abiotic factors
Provided is a method and system for comprehensive water quality assessment by integrating biotic and abiotic factors. The method includes: acquiring abiotic factors of a water body to be tested; constructing a biotic factor indicator library by an environmental DNA technology; determining a biotic-abiotic response relationship-based abiotic factor weight matrix using the abiotic factors and the biotic factor indicator library; acquiring a machine learning-based abiotic factor weight matrix using the abiotic factors and a LightGBM model; determining an abiotic factor comprehensive weight matrix according to the biotic-abiotic response relationship-based abiotic factor weight matrix and the machine learning-based abiotic factor weight matrix; and conducting the comprehensive water quality assessment of the water body to be tested based on the abiotic factor comprehensive weight matrix and the abiotic factors to determine a comprehensive water quality assessment result of the water body to be tested.
Owner:PEKING UNIV
Primer, probe and kit for identifying coreius guichenoti based on environmental DNA
The invention provides a primer, a probe and a kit for identifying coreius guichenoti based on environmental DNA, and a specific primer and a probe designed according to a COI gene sequence in coreius guichenoti mtDNA, namely the primer and the probe can specifically identify coreius guichenoti in numerous fishes, so that the detection time is greatly shortened, the detection can be completed within 6 hours, and the detection efficiency is greatly improved. The method is simple in operation, accurate in monitoring result and high in sensitivity, the lowest DNA concentration of real-time fluorescence PCR detection can reach the pg level, and the method better meets the actual requirements of eDNA field detection in the upstream of the Yangtze River.
Owner:SOUTHWEST UNIV
Method for double-target digital detection of environmental DNA (Deoxyribonucleic Acid) of Yangtze River cowfish and application
ActiveCN121450816AMicrobiological testing/measurementDNA/RNA fragmentationFinless porpoiseMicrobiology
The invention belongs to the technical field of biological detection, and particularly discloses a method for double-target digital detection of environmental DNA (Deoxyribonucleic Acid) of Yangtze River cowfish and application. According to the method, two pairs of specific primer probes are designed, synchronous identification of two target spots and accurate quantification of the copy number in a single reaction system are achieved, compared with a single target, the environment DNA detection rate of the Yangtze River cowfish is increased, and the method has extremely high species specificity, sensitivity and accuracy. Technical support and theoretical basis are provided for investigating the distribution situation of the field Yangtze River cowfish, and the method is of great significance to protection work of the Yangtze River cowfish. The method is strong in specificity and high in detection sensitivity, and can realize accurate capture of trace environmental DNA of the Yangtze globefish. Experimental data show that the lowest detection limit of the method can reach 0.2 copies / [mu] L, the detection rate under the concentration is 100%, and even under the extremely low concentration of 0.1 copies / [mu] L, the detection rate is still not lower than 80%.
Owner:INST OF AQUATIC LIFE ACAD SINICA
A method for monitoring and early warning of red tide algae based on environmental DNA
Owner:DALIAN OCEAN UNIV
Environmental DNA species monitoring method based on Chinese sturgeons
PendingCN120967002AClimate change adaptationMicrobiological testing/measurementBiotechnologyMicrobiology
The invention discloses an environmental DNA species monitoring method based on Chinese sturgeons, and relates to the technical field of biological gene detection.According to the environmental DNA species monitoring method based on the Chinese sturgeons, mitochondrial whole genomes of the Chinese sturgeons are determined through a method combining a second-generation library-building sequencing technology and a third-generation library-building sequencing technology, and the whole genomes of the mitochondria of the Chinese sturgeons are determined through probe design and PCR amplification sequencing. The sequence of the whole mitochondrial genome of the Chinese sturgeons is combined for comparison, and the specificity difference between the Chinese sturgeons and other related sturgeons is distinguished, so that the efficiency of detecting rare and endangered species Chinese sturgeons by an environmental DNA method is improved, and the accuracy of species identification is improved.
Owner:WUHAN UNIV
Probe primer and method for monitoring holothuria scabra based on environmental DNA technology
The application discloses a kind of probe primer and method based on environmental DNA technology to the probe of rough sea cucumber monitoring.The sequence of the primer is: Hs-F: 5'-GGAGTAAACTTAACCTTCTTC-3', Hs-R: 5'-GTCTGGGTAGTCTGAATAC-3';The sequence of the probe is: Hs-P: 5'-FAM-ATC(C)CC(G)CT(A)AG(C)CTA-BHQ1-3'.The method of the application determines the copy number of rough sea cucumber in water body under different sea areas environment, compares it with the standard curve of corresponding rough sea cucumber DNA copy number and Ct value and detection lower limit LOD, provides the existence condition of field rough sea cucumber, realizes the purpose of monitoring rough sea cucumber, and provides technical support for field distribution investigation of rough sea cucumber and relevant policy making.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI
Primer probe group and method for monitoring distribution of spotted seal based on environmental DNA (Deoxyribose Nucleic Acid)
The invention discloses a primer probe group and a method for monitoring distribution of spotted seal based on environmental DNA (Deoxyribonucleic Acid), and belongs to the technical field of molecular biology and environmental monitoring. The invention relates to a primer probe group for distribution monitoring of spotted seal based on environmental DNA (deoxyribonucleic acid). The primer probe group comprises a primer P-lar-ND2-F with a sequence as shown in SEQ ID No. 1, a primer P-lar-ND2-R with a sequence as shown in SEQ ID No. 2, and a probe P-lar-ND2-P with a sequence as shown in SEQ ID No. 3. The primer probe group comprises a primer P-lar-ND2-F, a primer P-lar-ND2-R, a primer P-lar-ND2-R, a primer P-lar-ND2-R and a probe P-lar-ND2-P, the method is applied to the aspect of distribution monitoring of the spotted seal, and has the characteristics of high specificity and high sensitivity.
Owner:FIRST INSTITUTE OF OCEANOGRAPHY MNR +1
DNA collection and analysis method suitable for coral reef area multi-source environment
The invention discloses a coral reef area multi-source environment DNA collection and analysis method. The method comprises the following steps: 1) collecting environmental samples from different sources in a coral reef area, wherein the environmental samples comprise surface water, near-bottom water, a large water body, pore water, sediments and the like; the method comprises the following steps: adding internal reference DNA (danio rerio DNA) into an environmental sample, establishing a positive control sample containing the internal reference DNA (danio rerio DNA) and external reference DNA (oryzias latipes DNA), and extracting DNA in the environmental sample, the positive control sample and the negative control sample by using an improved environmental DNA extraction method; and 2) performing homogenization treatment on data generated by high-throughput sequencing of the DNA sample extracted in the step 1) to realize parallel comparison or combined analysis of different samples. Through parallel comparison and combined analysis of environment DNAs from different sources, species diversity and distribution conditions of coral reefs are reflected more comprehensively.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI
An environmental DNA-based rare and endangered aquatic organism monitoring and alarming device
This invention relates to the field of biodiversity monitoring technology and discloses a monitoring and alarm device for rare and endangered aquatic organisms based on environmental DNA. The device includes a sampling and enrichment unit, a nucleic acid in-situ extraction and purification unit, a isothermal fluorescence detection unit, an environmental factor monitoring unit, a main control and data transmission unit, a power supply and protection unit, and a remote alarm and control platform. The main control and data transmission unit obtains the pressure change rate based on the digital pressure signal output by the microcomputer-controlled pressure sensor, extracts the high-precision peristaltic pump operating time parameter to calculate the actual filtration volume, and performs a division operation to obtain the environmental DNA calibration concentration. It then calculates the dynamic transmission power value by combining the network signal strength indicator value and transmits the complete communication data packet. The remote alarm and control platform obtains a risk index based on the environmental DNA calibration concentration, maps the spatial coordinate signal to an electronic map module to generate coordinate nodes, and displays the risk index and digital environmental parameters in association.
Owner:YUNNAN ACAD OF ENVIRONMENTAL SCI
Intelligent diagnosis method for estuary habitat degradation
The present application relates to the technical field of intelligent diagnosis, in particular to a kind of estuary habitat degradation intelligent diagnosis method, comprising S1: obtaining the in-situ time series monitoring data of estuary key section;S2: extract tidal characteristic phase, carry out tidal phase normalization processing, generate standard ecological dataset;S3: standard ecological dataset and the biodiversity index obtained based on environmental DNA technology are spatiotemporal fusion;The response relationship between the ecological parameters in different tidal characteristic phase interval and biodiversity index is modeled, and the response lag feature and coupling strength of each ecological parameter are extracted;Multi-dimensional local dynamic baseline is constructed;S4: time lag compensation is carried out to each dimension index;Based on the index value after time lag compensation, the standardization deviation degree of each dimension index is calculated, and the comprehensive deviation index is obtained by weighted integration;S5: identify the dominant type leading to habitat degradation.The present application improves the accuracy and efficiency of ecological monitoring and management.
Owner:FIRST INSTITUTE OF OCEANOGRAPHY MNR
A collection device for monitoring fish community based on environmental DNA technology
ActiveCN120404239BControl the ups and downsEasy to sampleWaterborne vesselsWithdrawing sample devicesFisheryPropeller
The present application relates to the technical field of robots, and especially relates to a collection device for monitoring fish community based on environmental DNA technology, which comprises a sampling device and a sample collecting device, liquid samples are sucked through the sampling device, the liquid samples are stored in the sample collecting device after being delivered, the floating and sinking state of the collection device is controlled through the floating device, so that the collection device is suspended in water, the expansion state of the collection device is controlled through the shape adjusting device, the suspension height of the collection device in water is adjusted, and the screw propeller assembly is arranged on both sides of the shape adjusting device, the movement of the collection device in water is controlled through the cooperation of the screw propeller assembly and the shape adjusting device. The device can make the whole collection device float on the water surface, sample the water area at the surface shallow position of the water body, and also control the floating and sinking state of the whole collection device, so that the collection device is suspended at different heights in water, sampling in multiple positions and multiple aspects is facilitated, and the diversity of sampling is increased.
Owner:TIBET AUTONOMOUS REGION INST OF PLATEAU BIOLOGY
RPA-CRISPR / Cas12a system and specific primer for detecting cowfish based on environmental DNA (Deoxyribose Nucleic Acid)
PendingCN121737315AMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyHigh activity
The invention discloses an RPA-CRISPR / Cas12a system for detecting a cowfish based on environmental DNA (Deoxyribonucleic Acid) and a specific primer, and belongs to the technical field of biological detection. Aiming at the sequence of a COI fragment of a mitochondrial genome of the Jiangsu, an RPA primer with the species specificity of the Jiangsu is designed so as to amplify a target fragment; designing a high-activity and specific crRNA sequence by searching a Cas12a protein recognition site in a corresponding amplification sequence so as to guide the Cas12a protein to recognize a target fragment and activate the trans-cleavage activity of the Cas12a protein; by designing a molecular probe with stable performance, the probe is stably and efficiently cut by a Cas12a-crRNA active compound, and a detection result can be observed in real time through a luminoscope. The detection method is easy to operate, high in sensitivity and good in specificity, detection can be completed only through constant-temperature heating equipment, and a new technology and method are provided for rapid detection and protection of the cowfish.
Owner:YUNNAN UNIV +1
Primer probe set for detecting leiognathus bindus environment DNA, detection method and application
The invention relates to the technical field of molecular biology, in particular to a primer probe set for detecting leiognathus bindus environment DNA, a detection method and application. The primer probe group comprises a specific primer and a specific fluorescent probe; wherein the specific primer comprises a forward primer NC-F, a reverse primer NC-R and a specific fluorescent probe NC-Taq. The primer probe group provided by the invention can accurately distinguish leiognathus bindus and related species thereof and common fishes in yellow and Bohai Sea, and has strong specificity. According to the invention, a real-time fluorescence qualitative and / or quantitative PCR (qPCR) detection method for the leiognathus bindus environment DNA is established, and high-sensitivity quantitative detection of a trace amount of the leiognathus bindus environment DNA in a water body can be realized. The method can be directly applied to field population distribution investigation and expansion trend tracking of Yellow and Bohai sea expansion species leiognathus bindus, so that theoretical support is provided for dynamic management of fishery resources and early warning of marine ecological risks.
Owner:OCEAN UNIV OF CHINA
Method for biodiversity survey and monitoring based on satellite remote sensing and environmental DNA sequencing
The application discloses a biodiversity investigation and monitoring method based on satellite remote sensing and environmental DNA sequencing, comprising the following steps: S1, obtaining habitat remote sensing data and environmental DNA data; S2, preprocessing the obtained data and comparing with a public gene database to obtain species distribution data; S3, aligning and fusing the habitat remote sensing data and the environmental DNA data in time and space scales to generate a biodiversity feature data set; and S4, constructing a correlation model and a three-dimensional model between the habitat and the species distribution, and visually displaying the dynamic change of biodiversity. The application improves the comprehensiveness, accuracy and timeliness of investigation and monitoring, and provides a scientific basis for biodiversity protection.
Owner:CHINESE RES ACAD OF ENVIRONMENTAL SCI
Guided fence installation area derivation system through analysis of vulnerability to harmful birds and animals, and guided fence installation area derivation method using same
The present invention relates to a technology capable of efficiently deriving a place to install an induction fence intended for blocking the invasion of harmful birds and animals, wherein an optimal location may be calculated in such a manner as to calculate, with respect to animal species and inhabitant species shown in an area where the harmful birds and animals appear, an index for vulnerability based on image data and information resulting from analyzing environmental DNA, and to match LiDAR scanning data based on the vulnerability index.
Owner:SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION
Method for synchronously and quantitatively determining epithelial cells of Atlantic salmon, karenia mikimotoi and vibrio parahaemolyticus in seawater
PendingCN120888644AMicrobiological testing/measurementMicroorganism based processesEpitheliumVibrio parahaemolyticus
The invention relates to a method for synchronously and quantitatively determining Atlantic salmon epithelial cells, Karenia mikimotoi and Vibrio parahaemolyticus in seawater, and belongs to the technical field of detection. Respectively adding the extracted eDNA into a reaction tube of an LAMP reaction system filled with a targeted Atlantic salmon marker gene Cytb, a Karenia mikimotoi marker gene ITS2 and a Vibrio parahaemolyticus marker gene tlh, and then putting the reaction tube into a full-automatic electronic gene amplification analyzer to obtain slopes kf, ka and kb of a linear fitting equation corresponding to a kinetic curve exponential phase, and substituting the Atlantic salmon epithelial cells, the Karenia mikimotoi and the vibrio parahaemolyticus into formulas LogNf = 52.96 kf + 0.27, LogNa = 88.13 ka-0.54 and LogNb = 70.79 kb < 2 > + 35.67 kb + 0.70, and respectively calculating the numbers Nf, Na and Nb of the Atlantic salmon epithelial cells, the Karenia mikimotoi and the vibrio parahaemolyticus in the detected seawater sample. According to the method, the number of target animal cells, algae cells and microbial cells can be synchronously obtained within 2 hours.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Environmental DNA tracer agent and tracing method for hydrological survey and pollutant traceability
The invention discloses an environmental DNA tracer for hydrological survey and pollutant traceability and a tracing method, and belongs to the technical field of environmental monitoring. The tracer agent comprises a carrier matrix and free DNA encapsulated in the carrier matrix, and the carrier matrix is selected from one of silicon dioxide microspheres and sodium alginate-chitosan composite hydrogel microspheres according to drainage basin water diversion tracing scene requirements; the free DNA is a specific double-stranded DNA fragment which is artificially designed and synthesized, and meets the uniqueness and detectability of the sequence. The tracer agent is environmentally friendly, high in specificity and high in sensitivity, the tracing method is easy and convenient to operate, the water flow migration track and the pollutant diffusion range can be accurately analyzed, and the tracer agent is suitable for tracing monitoring under the complex hydrological condition.
Owner:NANJING HYDRAULIC RES INST +2
Underwater in-situ environment DNA sampling and water quality monitoring integrated device and sampling method
PendingCN121917731ABioreactor/fermenter combinationsBiological substance pretreatmentsAutomatic controlFiltration
According to the underwater in-situ environment DNA sampling and water quality monitoring integrated device and the sampling method provided by the invention, in-situ filtration is taken as a core, and water sample carrying is reduced. The device can be immersed in water, and water is directly taken and filtered in a ship-borne winch, a fish raft, a net cage or a near-shore scene; an ex-situ mode is also supported, a transferred water sample is filtered in a laboratory or a ship, and the universality of equipment is improved. The independent detachable filter membrane box is adopted, pipelines are shortened, and the risk of cross contamination is reduced. A water quality sensor is integrated at the front end, a controller synchronously records water quality parameters, and environment DNA is matched with environment background data. The system has an automatic control function, starts and stops the water pump according to a preset program, estimates the filtering volume, and automatically records sampling time, depth, position and water quality data; and the GNSS module is combined to obtain longitude and latitude, so that the space traceability of an environment DNA result is enhanced.
Owner:XIAMEN UNIV
Blocking agent of environmental DNA for eliminating human pollution and detection method of environmental DNA
PendingCN121344174AMicrobiological testing/measurementDNA/RNA fragmentationDNA ContaminationBio informatics
The invention discloses a blocking agent for eliminating environmental DNA (Deoxyribose Nucleic Acid) of human pollution and a detection method of the environmental DNA, and the blocking agent plays a role in inhibiting amplification of a human source sequence by specifically hybridizing with a human source template and blocking primer annealing or DNA polymerase extension. The invention further provides application of the CRISPR-Cas12a technology to in-vitro removal of human-derived DNA pollution, the method can reduce the sequencing cost and the time cost of bioinformatics processing without affecting detection of target biological groups, and the method is mainly used for treating human pollution in marine environment DNA. And the method can also be used for treating human-derived DNA pollution in similar molecular biology research and application.
Owner:GUANGDONG UNIV OF TECH
An environmental DNA filtering filter element, enricher, enrichment device and sampling method
ActiveCN120173711BSemi-permeable membranesBioreactor/fermenter combinationsEnvironmental DNAEnvironmental monitoring
The application belongs to the technical field of environmental monitoring, and relates to an environmental DNA filtering filter core, an enriching device, an enriching device and a sampling method. In view of the technical problem that the filtering device in the prior art has a small volume of environmental DNA enrichment, low enrichment efficiency and easy sample pollution, and thus a small number of detected species, the application provides an environmental DNA filtering filter core, which comprises filter layers and two head end covers, the filter layers are located between the two head end covers and connected with the two head end covers, the filter layers are folded for multiple times, so that the cross section of the filter layers is arranged in a wave shape, and the number of folding layers of the filter layers is 40-65. The enrichment rate of the sample is increased, a high elution rate can be maintained, and the number of detected species is increased. The application further provides an environmental DNA enriching device and an enriching device, which realize multi-channel, large-capacity and rapid simultaneous enrichment of samples, and reduce sample pollution. The application further provides an environmental DNA sampling method, which adopts continuous underway sampling and is suitable for large-basin water environment.
Owner:YUNNAN UNIV +2
Method for double-target digital detection of Yangtze finless porpoise environmental DNA and application
ActiveCN121450816BMicrobiological testing/measurementDNA/RNA fragmentationCurrent distributionFinless porpoise
This invention belongs to the field of biodetection technology, specifically disclosing a method and application for dual-target digital detection of environmental DNA of the Yangtze finless porpoise. This method, through the design of two pairs of specific primer probes, achieves simultaneous identification and precise copy number quantification of two targets in a single reaction system. Compared with single-target detection, it increases the detection rate of Yangtze finless porpoise environmental DNA, exhibiting extremely high species specificity, sensitivity, and accuracy. This invention provides technical support and theoretical basis for conducting surveys on the current distribution status of wild Yangtze finless porpoises, and is of great significance to the conservation of the Yangtze finless porpoise. This method has high specificity and high detection sensitivity, enabling the precise capture of trace amounts of Yangtze finless porpoise environmental DNA. Experimental data show that the limit of detection of this method can reach 0.2 copies / μL, and the detection rate at this concentration is 100%. Even at extremely low concentrations of 0.1 copies / μL, the detection rate is still not less than 80%.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Dynamic monitoring and early warning system and method for diversity of aquatic organisms in watershed
The invention provides a watershed aquatic organism diversity dynamic monitoring and early warning system and method, and belongs to the field of watershed ecological protection, the watershed aquatic organism diversity dynamic monitoring and early warning system comprises a data acquisition module used for acquiring a watershed surface water sample to obtain environmental DNA sampling data; the data analysis module is used for carrying out recognition by adopting a kernel principal component analysis method to obtain a nonlinear ecological relationship among species, and constructing a multi-scale ecological response coupling model to obtain a multi-scale ecological response characteristic and environmental factor association relationship of aquatic organisms in the watershed; the analysis and prediction module is used for calculating a basin biological integrity index and an accumulated pressure index, and performing time sequence analysis and trend prediction to obtain a basin aquatic organism diversity prediction result; and the early warning judgment module is used for establishing a classification early warning threshold calculation system and carrying out comprehensive early warning state judgment based on the basin biological integrity index and the accumulated pressure index. According to the invention, the accuracy, the timeliness and the coverage range of the dynamic monitoring of the diversity of the aquatic organisms in the watershed can be improved.
Owner:CHINA HYDROELECTRIC ENGINEERING CONSULTING GROUP CHENGDU RESEARCH HYDROELECTRIC INVESTIGATION DESIGN AND INSTITUTE +1