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56 results about "Environmental DNA" patented technology

Environmental DNA or eDNA is DNA that is collected from a variety of environmental samples such as soil, seawater, snow or even air rather than directly sampled from an individual organism. As various organisms interact with the environment, DNA is expelled and accumulates in their surroundings. Example sources of eDNA include, but are not limited to, feces, mucus, gametes, shed skin, carcasses and hair. Such samples can be analyzed by high-throughput DNA sequencing methods, known as metagenomics, metabarcoding, and single-species detection, for rapid measurement and monitoring of biodiversity. In order to better differentiate between organisms within a sample, DNA metabarcoding is used in which the sample is analyzed and uses previously studied DNA libraries to determine what organisms are present (e.g. BLAST). The analysis of eDNA has great potential, not only for monitoring common species, but to genetically detect and identify other extant species that could influence conservation efforts. This method allows for biomonitoring without requiring collection of the living organism, creating the ability to study organisms that are invasive, elusive, or endangered without introducing anthropogenic stress on the organism. Access to this genetic information makes a critical contribution to the understanding of population size, species distribution, and population dynamics for species not well documented. The integrity of eDNA samples is dependent upon its preservation within the environment. Soil, permafrost, freshwater and seawater are well-studied macro environments from which eDNA samples have been extracted, each of which include many more conditioned subenvironments. Because of its versatility, eDNA is applied in many subenvironments such as freshwater sampling, seawater sampling, terrestrial soil sampling (tundra permafrost), aquatic soil sampling (river, lake, pond, and ocean sediment), or other environments where normal sampling procedures can become problematic.

Environment DNA identification method for fish community structure researching

The invention relates to an environment DNA identification method for fish community structure researching. The method is characterized by comprising the steps that 1, water samples are collected according to the size of the water area where a fish community is located; 2, the water samples are processed, and total environment DNA of the processed water samples is extracted; 3, a 16s r DNA sequence with nucleotide sequences being SEQ ID NO:1 and SEQ ID NO:2 is adopted as a universal primer to carry out PCR amplification on the total environment DNA, and a PCR product is obtained; 4, gel electrophoresis is carried out on the PCR product, and gel with DNA is obtained; 5, gel cutting and clone sequencing are carried out on the gel with the DNA, and then blast comparison is carried out through GENBANK so as to determine whether the DNA is a fish sequence or not; 6, after it is determined that the DNA is the fish sequence, the next-generation sequencing technology is adopted for analyzing composition conditions of the PCR product on a large scale, and therefore the environment DNA fish composition and community structure are determined. The environment DNA identification method is quite easy, convenient and efficient and has the practical value.
Owner:SHANGHAI OCEAN UNIV

Conjoint analysis method for estimating DNA abundance of fishes based on environment DNA technology

The invention relates to a conjoint analysis method for estimating the DNA abundance of fishes based on an environment DNA technology. The conjoint analysis method includes the steps that the total DNA abundance of the fishes is acquired through timed and quantified PCR; the species composition proportion of the fishes is acquired through 454 GS-FLX Titanium sequencing analysis; conjoint analysis of target species is performed through sequencing data and timed and quantified PCR data; a relative distribution diagram of mitochondrial DNA of the fishes in an environment is drawn through sufer 8.0 software. Based on the environment DNA identification technology, weather species exist and how many species exist can be analyzed just by collecting water samples without depending on fish species capturing, a sampling method is simple, and fish resources can be protected to the maximum degree; meanwhile, for some species which are rarely distributed and difficult to capture, the method is still more effective than traditional fishing investigation, the method can effectively solve the problem that effective species specificity primers are difficult to obtain when multiple closely related species exist in samples, and NDA abundance information of all species can be known just by conducting experiment once respectively through the two technologies.
Owner:SHANGHAI OCEAN UNIV

Method for detecting diversity of large aquatic plants based on environmental DNA technology

The invention discloses a method for detecting diversity of large aquatic plants based on an environmental DNA technology. The method for detecting the diversity of the large aquatic plants in a waterarea to be detected comprises the following steps: 1) collecting a surface sediment sample of the water area to be detected; 2) extracting environmental DNA (eDNA) in the sediment sample; 3) amplifying the eDNA sample by using an Rbcl primer to obtain a PCR product, and recovering and purifying the product to obtain a purified PCR product; and 3) sequentially performing database building, sequencing, clustering and annotation on the purified PCR product to determine the species composition and/or the species relative abundance of the large aquatic plants in the water area to be detected, so as to realize the detection of the diversity of the large aquatic plants in the water area to be detected. The method disclosed by the invention has the following advantages: the sampling is simple andfast; the detection is efficient, the precision is high, and the detection result is accurate; and the large aquatic plants do not need to be collected, so that the aquatic plant population is protected, and particularly the protection of rare and endangered species is realized.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI

Environmental DNA macro bar code method for researching macrobenthic animal community structure

The invention discloses an environmental DNA macro bar code method for researching a macrobenthic animal community structure. One technical scheme to be protected by the invention is the application of the primer pair in detecting the community structure of the macrobenthos in the water area to be detected. The primer pair is named as COI and is composed of a primer 1 and a primer 2, and the primer 1 and the primer 2 can be a sequence 1 and a sequence 2 in a sequence table. Compared with a traditional method, the COI primer provided by the invention is used for amplifying an environmental DNAsample of a to-be-detected water area, and sequencing and annotation analysis are carried out to obtain more obvious species, especially benthonic animal groups which are easy to escape, small in sizeand partial in life history in water; and the method can supplement benthonic animal abundance data which are easy to ignore, difficult to find and small in size in a traditional investigation method, effectively improves the identification level, species detection rate and abundance evaluation accuracy of the large benthonic animals, and is an effective and reliable new method for investigatingthe community structure of the large benthonic animals.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI

Method for evaluating integrity of benthonic animals based on environmental DNA macro bar code technology

The invention discloses a method for evaluating biological integrity of benthonic animals of a freshwater ecosystem based on environmental DNA macro bar code monitoring data, and belongs to the technical field of biology. Based on the results of monitoring of the benthonic animals through environmental DNA macro bar codes, the method comprises a series of monitoring and evaluation processes including (1) determining a reference point and a damaged point; (2) collecting an environmental DNA sample; (3) performing biological monitoring on an eDNA macro bar code; (4) defining and calculating candidate biological indexes based on the eDNA macro bar code monitoring; (5) screening the candidate indexes; (6) selecting the biological indexes to calculate the biological integrity score of the benthonic animals; and (7) dividing health levels and the like. The method provides complete and easy-to-operate guidance for the evaluation of the biological integrity of the benthonic animals of the freshwater, and can be widely applied to the evaluation of the integrity of the benthonic animals and the evaluation of the water ecological health condition of freshwater ecosystems such as streams, rivers, lakes and reservoirs.
Owner:NANJING UNIV

Method for monitoring and evaluating fish passage effects based on environmental DNA

The invention discloses a method for monitoring and evaluating fish passage effects based on environmental DNA. The method comprises the following steps: S1, setting three sampling points at an entrance, exit and rest pool of a fish passage, and collecting water samples and performing storage; S2, performing suction filtration on the collected water samples, and then performing extraction of totalenvironmental DNA; S3, using the extracted total environmental DNA as a template, and using designed primers to PCR amplify a 16S rDNA sequence to obtain a PCR product; S4, recovering and purifying the PCR product, and performing high-throughput sequencing to obtain a PCR product sequence; S5, establishing a fish 16S rDNA sequence alignment database; S6, searching for homologous sequences in theNCBI database and the established database through BLAST, and determining fish species information; and S7, counting the obtained fish species information, and completing the monitoring and evaluationof the fish passage effects. Compared with other existing methods, the method does not need to carry out a large number of fishing operations or instrument operations, is simple in sampling, cannot cause harms to fish, and thereby is conducive to the protection of fish.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI

Rare aquatic organismwater sampleenvironment DNA (Deoxyribonucleic Acid) optimization-cultivationwater sample pre-detection method

The invention discloses a rare aquatic organismwater sampleenvironment DNA (Deoxyribonucleic Acid) optimization-cultivationwater sample pre-detection method, which comprises the steps of before carrying out endangered aquatic species distribution investigation, making a perfect implementation plan through pre-detection; through acquiring an aquaculture pond water sample, and by taking detection of a target specie as an aim, analyzing water sample eDNA. Compared with water samples in a laboratory and an aquarium, according to the method, the physicochemical propertyof a water sample of an aquaculture pond constructed around a habitat has higher similarity to a water flow of an inhabiting area of species, so that the aquaculture pond water sample is utilized for simulating a habitat water sample, eDNA analysis process optimization of the target specie is carried out, and the reliability and the practicability of a determined operation flow are higher. According to the rare aquatic organismwater sampleenvironment DNA optimization-cultivationwater sample pre-detection method provided by the invention, the work efficiency of aquatic (semi-aquatic) species distributioninvestigation can be effectively improved, the work consumption is reduced, meanwhile, the reliability and the repeatability of an investigated result are ensured, and the sharing and the compatibility of different-source data are realized.
Owner:SHAANXI INST OF ZOOLOGY NORTHWEST INSTOF ENDANGERED ZOOLOGICAL SPECIES

Procypris rabaudi real-time fluorescent PCR (polymerase chain reaction) amplification primer, probe and detection method based on environmental DNA (deoxyribonucleic acid)

The invention discloses a composition of a primer and a probe for procypris rabaudi real-time fluorescent PCR (Polymerase Chain Reaction) detection based on environmental DNA (Deoxyribose Nucleic Acid), and establishes a real-time fluorescent PCR detection method of procypris rabaudi to achieve the purpose of accurately identifying procypris rabaudi. A specific primer is designed according to the gene sequence of 12SrRNA in mitochondrial DNA of procypris rabaudi, the nucleotide sequence of an upstream primer YYLF1 is as shown in SEQ ID No. 1, the nucleotide sequence of a downstream primer YYLR1 is as shown in SEQ ID No. 2, and a probe YYL-MGB1 has the nucleotide sequence as shown in SEQ ID No. 3. The probe can be applied to qualitative detection of procypris rabaudi in a water body only by collecting a water sample of a water area in which the procypris rabaudi live, and is high in specificity and good in timeliness; and the density of the procypris rabaudi in the environment can be quantitatively detected, and the biomass and time-space dynamics of the procypris rabaudi at different points can be reflected, so that a reference is provided for artificial release effect evaluation and resource management protection of the procypris rabaudi under the large background of procypris rabaudi without fishery in the Yangtze River.
Owner:SOUTHWEST UNIVERSITY

Intelligent collection device for environment DNA in marine water body

The invention provides an intelligent collection device for environmental DNA in a marine water body, and relates to the technical field of DNA collection devices. The intelligent collection device for environment DNA in marine water comprises a remote control ship, the remote control ship is provided with a pull rope and a winding and unwinding assembly used for winding and unwinding the pull rope, one end of the pull rope is connected with the winding and unwinding assembly, and the other end of the pull rope is provided with a sampling barrel; a water storage cavity is formed in the sampling barrel, a first opening communicated with the water storage cavity is formed in the top of the sampling barrel, and a first electromagnetic valve is arranged at the first opening; a second opening communicated with the water storage cavity is formed in the bottom of the sampling barrel, and a second electromagnetic valve is arranged at the second opening; and the intelligent collection device further comprises a processor, a power source and a remote control module, the remote control module is wirelessly connected with the processor, and the retractable assembly, the power source, the first electromagnetic valve and the second electromagnetic valve are all electrically connected with the processor. By the adoption of the collecting device, marine water samples can be conveniently collected, operation is convenient and fast, and manpower is saved.
Owner:HAINAN UNIVERSITY
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