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140 results about "Environmental DNA" patented technology
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Environmental DNA or eDNA is DNA that is collected from a variety of environmental samples such as soil, seawater, snow or even air rather than directly sampled from an individual organism. As various organisms interact with the environment, DNA is expelled and accumulates in their surroundings. Example sources of eDNA include, but are not limited to, feces, mucus, gametes, shed skin, carcasses and hair. Such samples can be analyzed by high-throughput DNA sequencing methods, known as metagenomics, metabarcoding, and single-species detection, for rapid measurement and monitoring of biodiversity. In order to better differentiate between organisms within a sample, DNA metabarcoding is used in which the sample is analyzed and uses previously studied DNA libraries to determine what organisms are present (e.g. BLAST). The analysis of eDNA has great potential, not only for monitoring common species, but to genetically detect and identify other extant species that could influence conservation efforts. This method allows for biomonitoring without requiring collection of the living organism, creating the ability to study organisms that are invasive, elusive, or endangered without introducing anthropogenic stress on the organism. Access to this genetic information makes a critical contribution to the understanding of population size, species distribution, and population dynamics for species not well documented. The integrity of eDNA samples is dependent upon its preservation within the environment. Soil, permafrost, freshwater and seawater are well-studied macro environments from which eDNA samples have been extracted, each of which include many more conditioned subenvironments. Because of its versatility, eDNA is applied in many subenvironments such as freshwater sampling, seawater sampling, terrestrial soil sampling (tundra permafrost), aquatic soil sampling (river, lake, pond, and ocean sediment), or other environments where normal sampling procedures can become problematic.
Marine biological diversity protection method based on ecological dynamic model
PendingCN120217450AFinanceDatabase distribution/replicationEnvironmental resource managementEcological data
The invention relates to the technical field of marine organism protection, in particular to a marine organism diversity protection method based on an ecological dynamic model, which comprises the following steps: collecting biological and non-biological data in a marine ecosystem, extracting DNA from a seawater sample by utilizing an environmental DNA technology, and constructing the ecological dynamic model based on the collected ecological data and eDNA analysis result. According to the method, the ecological dynamics model is established, the collected data is stored and verified through the block chain technology, and a marine biological diversity protection scheme is designed and implemented according to the ecological dynamics model prediction result and the block chain verification data. Scientific support is provided for protection decision making, protection measures and management regulations are automatically executed through the intelligent contract technology, the system is allowed to respond immediately when it is detected that key environment variables or biological indexes change, and the flexibility and timeliness of responding to environment changes are greatly improved.
Owner:FIRST INSTITUTE OF OCEANOGRAPHY MNR
Enhancement and release seedling resource class evaluation method based on environmental DNA polymerization analysis
PendingCN121331216AData processing applicationsMicrobiological testing/measurementDNA extractionHigh throughput sequence
The invention discloses a method for evaluating enhancement and release seedling resources based on environmental DNA polymerization analysis. The method comprises the following steps: carrying out gridding partition on a target water area, collecting a water sample through a designed sampling scheme, and carrying out DNA extraction and high-throughput sequencing to obtain species sequence information of each sampling point. Sequencing data is subjected to species identification by using a bioinformatics method, released species are identified, a spatial abundance model is established, and a preliminary distribution map is generated. And establishing a DNA degradation kinetic model in combination with water area environmental parameters, and carrying out reverse correction on abundance distribution. And through a resource inversion model coupled with hydrodynamics, analyzing biomass distribution characteristics and migration laws of the release group, and obtaining a resource evaluation result. And finally, a species environment preference model is constructed based on migration path analysis, an optimal release area is matched in a target water area, a scientific scheme including release point locations, opportunities and quantity is generated, and a whole-process technical support and a decision basis are provided for enhancement and release.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
Pelteobagrus vachelli population dynamic monitoring system and method
The invention discloses a Pelteobagrus vachelli population dynamic monitoring system and method, particularly relates to the technical field of fishery resource monitoring, and aims to solve the problems of inaccurate juvenile fish survival rate evaluation and poor monitoring timeliness in a benthic hidden environment in the prior art. The method comprises the following steps: arranging a benthic penetration type sonar array to synchronously collect a water body reflection signal and a benthic environment sample, and extracting juvenile fish specific gene fragments by combining an environment DNA (Deoxyribose Nucleic Acid) enrichment technology to generate concentration distribution data; screening juvenile fish distribution data by using an acoustic reflection signal and body length correlation model, and positioning an active hot spot area through space-time overlapping analysis; deploying a directional sound wave excitation device to obtain escape trajectory data, and calculating a survival rate in combination with a density attenuation model; finally, multi-source data and topographic features are fused to generate a real-time population dynamic monitoring result, and high-precision and non-intrusive monitoring of the juvenile fish population in the hidden environment is realized.
Owner:FISHERIES RES INST ANHUI ACAD OF AGRI SCI
A method for predicting river aquatic biodiversity based on remote sensing and environmental DNA
The present invention discloses a method for predicting river aquatic biodiversity based on remote sensing and environmental DNA. The method comprises the following steps: determining the abundance of a first species at each sampling point in the entire river basin based on the environmental DNA of each sampling point; determining the patch corresponding to each sampling point based on the sampling point and the species activity range, and determining the first remote sensing index based on the pre-acquired remote sensing vegetation index and the corresponding first species abundance in the patch; training a first preset model based on the first remote sensing index and the corresponding first species abundance in each patch, and determining the first preset model as a species abundance model when the first preset model reaches a preset standard; dividing the entire river basin into multiple patches, and determining a second remote sensing index in each patch, wherein the second remote sensing index is determined by the remote sensing vegetation index corresponding to the first remote sensing index; inputting the second remote sensing index in each patch into the species abundance model, and determining the second species abundance in the entire river basin, so as to determine the species diversity in the entire river basin.
Owner:GUANGDONG UNIV OF TECH
Real-time certification and lifecycle automation of environmental credits
A system for real-time certification and lifecycle automation of environmental credits. The system comprises: (i) a physical data collection layer equipped with on-land sensors, aerial LiDAR, satellite imagery, environmental DNA (eDNA) sampling, and community-contributed field data; (ii) an Ecological Oracle Engine, which uses an AI protocol to validate, classify, and interpret multi-source ecological data, supported by an adaptive reference library; and (iii) a smart contract protocol deployed on a blockchain network, configured to autonomously convert verified ecological data into financial instruments by issuing, pricing, transferring, freezing, and retiring tokenized environmental credits. The system performs continuous verification of each credit and includes freezing functionality based on ecological performance. It supports multiple credit types, including but not limited to carbon, biodiversity, and water credits. The system may be integrated with external modules for integrates with a Digital Twin of Nature and Web3 ecosystems, including decentralized marketplaces, ReFi platforms, and DAO mechanisms.
Owner:SYTNYK OLHA
Method for detecting apple snails
PendingJP2025151531ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyPomacea diffusa
To detect apple snails from environmental DNA with a high detection rate.SOLUTION: This method amplifies and detects environmental DNA in a sample with a first primer having a specific base sequence, a second primer having another specific base sequence, and a probe having still another base sequence.SELECTED DRAWING: None
Owner:NAT AGRI & FOOD RES ORG
Microbial indicator species screening method supporting water resource scheduling decision
The invention relates to a microbial indicator species screening method for supporting a water resource scheduling decision, which is used for screening representative microbial indicators by combining an environmental DNA (eDNA) technology and monitoring the dynamic change of the microbial indicators so as to monitor and evaluate the diffusion range and process of the indicators and provide comprehensive support for the water resource scheduling decision. The blank of related methods is filled, and the ecological benefit, the environmental benefit and the economic benefit of water resource scheduling are comprehensively improved.
Owner:TAIHU BASIN HYDROLOGY & WATER RESOURCES MONITORING CENT (TAIHU BASIN WATER ENVIRONMENT MONITORING CENT) +1
Phytoplankton spatial distribution prediction method based on environmental DNA and machine learning
PendingCN120354761AEnsemble learningMicrobiological testing/measurementEcological environmentData set
The invention discloses a phytoplankton space distribution prediction method based on environment DNA and machine learning, and belongs to the technical field of ecological environment evaluation and treatment. Comprising the following steps: determining a sampling site, and collecting a phytoplankton eDNA sample; carrying out DNA extraction and treatment on the collected eDNA sample, and calculating a species abundance matrix; screening a training data set for training a prediction model according to the sampling time and the sampling site of the eDNA sample; inputting the training data set as an independent variable of a training set, inputting the species abundance matrix as a dependent variable of the training set, and training a prediction model; and inputting remote sensing image data into the prediction model to obtain a species spatial distribution diagram. Compared with the prior art, the method has the advantages that efficient and high-species-resolution spatial distribution prediction of algae species such as blue-green algae can be achieved, the phytoplankton prediction model is trained through the machine learning model, automatic operation of spatial prediction of various phytoplanktons is achieved, the arrangement density of sampling points is reduced, and the monitoring efficiency is improved.
Owner:YUNNAN UNIV +2
Primer and method for quantitatively monitoring biomass of sargassum hemiphyllum based on environmental DNA (Deoxyribose Nucleic Acid) technology
ActiveCN121737340AMicrobiological testing/measurementDNA/RNA fragmentationSargassum hemiphyllumDeoxyribose
The invention discloses a primer and a method for quantitatively monitoring the biomass of sargassum hemiphyllum based on an environmental DNA technology, and belongs to the technical field of molecular ecology. The primer probe group comprises an upstream primer, a downstream primer and a fluorescent probe which are specifically targeted to the sargassum hemiphyllum mitochondria COX1 gene, and the sequence is shown as SEQ ID NO.1-3. The kit comprises the primer probe group, a qPCR (quantitative polymerase chain reaction) premixed solution, nuclease-free water and a sargassum hemiphyllum plasmid positive control. The method comprises the following steps: collecting a water sample, enriching eDNA, performing qPCR detection by using the primer probe group after extraction and purification, and realizing qualitative detection and quantitative evaluation of sargassum hemiphyllum through a Cq value or a standard curve. The method disclosed by the invention has the advantages of high sensitivity, strong specificity, no damage to the environment and target organisms, capability of realizing large-scale rapid general survey and the like, and is suitable for early warning of gulfweed blooms, investigation of population distribution and evaluation of ecological influence.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI
Minimum population wild plant identification and early warning method and system
The invention discloses a minimum population wild plant identification and early warning method and system. The system comprises: an environmental DNA monitoring network unit, which analyzes environmental DNA through automatic sampling, evaluates the state of species and triggers a first early warning; the biological voiceprint monitoring and recognition unit is used for monitoring related acoustic activities and illegal intrusion behaviors of the target species by using intelligent recording nodes deployed in a gridding manner and a voiceprint recognition model, and triggering second early warning; and the multi-source information fusion and causal inference unit is used for integrating the environment DNA, the voiceprint and the meteorological data, analyzing the causal relationship among the ecological factors based on a causal inference model, generating protection measures and outputting comprehensive early warning. According to the invention, all-weather, interference-free and precise monitoring of the extremely small population wild plants is realized, the problems of low efficiency, many blind areas, large interference and the like of a traditional monitoring mode are solved, and effective technical support is provided for protection of rare plants.
Owner:陕西省林业科学院
A comprehensive evaluation method and system for multi-source data management and correction of fish resources in watersheds.
This application discloses a comprehensive assessment method and system for multi-source data management and correction of watershed fish resources. The method includes: extracting multi-dimensional features from a first data matrix to obtain a first feature matrix; classifying species data based on anomaly scores, outputting verification results, and correcting the first feature matrix to obtain a second feature matrix; acquiring environmental DNA sequencing data and corresponding environmental data; obtaining a first eDNA dataset from the environmental DNA sequencing data through ecological screening; correcting the species in the first eDNA dataset to obtain a second eDNA dataset; fusing the second eDNA dataset with the second feature matrix; calculating biodiversity indices and analyzing community structure; analyzing long-term trends in population and community structure; and generating a comprehensive assessment report. This application achieves full-process automation and intelligence from data quality control and fusion to assessment, significantly improving the accuracy and efficiency of watershed fish resource assessment.
Owner:WUHAN UNIV
Marine benthic ecological environment quality assessment method based on environment DNA
PendingCN120249455AMicrobiological testing/measurementBiostatisticsEnvironmental resource managementEcological environment
The invention belongs to the field of ecological environment evaluation and treatment, and particularly relates to a marine benthic ecological environment quality evaluation method based on an environmental DNA technology. Comprising the following steps: 1) collecting an environmental DNA sediment sample; 2) extracting sediment environment DNA from the environment DNA sediment sample; (3) amplifying macro bar code fragments by taking the environmental DNA extracted in the step (2) as a template, and sequencing; (4) analyzing the species category of the sequencing result of the macro bar code in the step (3), and defining the macrobenthonic animals; and 5) evaluating the quality of the marine benthic ecological environment by combining the environment DNA macro bar code with AMBI. The environment DNA macro bar code technology is applied to marine benthonic habitat quality evaluation, and environment DNA PCR amplification, screening of large benthonic animals in sequence species, AMBI index data calculation and habitat quality evaluation grade technologies are defined; wherein screening of large benthonic animals in an environment DNA macro bar code sequence and introduction of sequence absolute abundance into AMBI calculation are key technologies for influencing a final marine benthonic habitat quality evaluation result.
Owner:BEIHAI FORECASTING CENT OF STATE OCEANIC ADMINISTRATION ((QINGDAO MARINE FORECASTING STATION OF STATE OCEANIC ADMINISTRATION) (QINGDAO MARINE ENVIRONMENT MONITORING CENT OF STATE OCEANIC ADMINISTRATION))
Filter assembly, kit and methods
The present invention is directed to a filter assembly for capturing environmental DNA (eDNA), a kit comprising the filter assembly, a method of capturing eDNA using the filter assembly, a method of analysing eDNA captured in the filter assembly, and a method of providing biodiversity data by analysing eDNA collected in the filter assembly.
Owner:NATURE METRICS LTD
An environmental DNA-based primer probe combination for oncorhynchus masonii and application thereof
The application discloses a primer probe combination for Salmo platycephalus based on environmental DNA and application thereof, and belongs to the technical field of molecular biology and endangered aquatic organism monitoring. The application provides the primer probe combination for Salmo platycephalus, which comprises upstream and downstream primers and a probe P, and the amplified fragment has a length of 179 bp; the primer probe combination for Salmo platycephalus can specifically detect Salmo platycephalus in environmental DNA, can efficiently monitor and protect Salmo platycephalus, and can effectively avoid the interference of related species including Salmo, Oncorhynchus and Leucichthys bampsii; the provided primer probe combination for Salmo platycephalus can be applied to the monitoring of Salmo platycephalus in different water bodies to be detected such as streams, rivers and reservoirs, is easy to popularize, and has important significance for the protection and monitoring of Salmo platycephalus.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Method and system for comprehensive water quality assessment by integrating biotic and abiotic factors
Provided is a method and system for comprehensive water quality assessment by integrating biotic and abiotic factors. The method includes: acquiring abiotic factors of a water body to be tested; constructing a biotic factor indicator library by an environmental DNA technology; determining a biotic-abiotic response relationship-based abiotic factor weight matrix using the abiotic factors and the biotic factor indicator library; acquiring a machine learning-based abiotic factor weight matrix using the abiotic factors and a LightGBM model; determining an abiotic factor comprehensive weight matrix according to the biotic-abiotic response relationship-based abiotic factor weight matrix and the machine learning-based abiotic factor weight matrix; and conducting the comprehensive water quality assessment of the water body to be tested based on the abiotic factor comprehensive weight matrix and the abiotic factors to determine a comprehensive water quality assessment result of the water body to be tested.
Owner:PEKING UNIV
Primer group for identifying phthorimaea operculella based on ddPCR and method for identifying phthorimaea operculella by detecting environmental DNA
PendingCN120624682AMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyForward primer
The invention discloses a primer group for identifying phthorimaea operculella based on ddPCR and a method for identifying phthorimaea operculella by detecting environmental DNA, and belongs to the technical field of pest detection. The primer group for identifying the phthorimaea operculella based on the ddPCR comprises a forward primer, a reverse primer and a specific fluorescent probe, the nucleic acid sequences of the forward primer, the reverse primer and the specific fluorescent probe are as shown in SEQ ID NO.1-SEQ ID NO.3; the C basic group at the 3'end of the forward primer, the A basic group at the 3 'end of the reverse primer, and the A basic groups and the T basic groups at the 12th site and the 13th site of the specific fluorescent probe are basic groups modified by locked nucleic acid. The primer group disclosed by the invention has very high specificity and sensitivity to the phthorimaea operculella, can be detected by taking eDNA as a template, can find the phthorimaea operculella earlier, and has an important application prospect in early detection and prevention of the phthorimaea operculella.
Owner:QINGDAO AGRI UNIV
Primer, probe and kit for identifying coreius guichenoti based on environmental DNA
The invention provides a primer, a probe and a kit for identifying coreius guichenoti based on environmental DNA, and a specific primer and a probe designed according to a COI gene sequence in coreius guichenoti mtDNA, namely the primer and the probe can specifically identify coreius guichenoti in numerous fishes, so that the detection time is greatly shortened, the detection can be completed within 6 hours, and the detection efficiency is greatly improved. The method is simple in operation, accurate in monitoring result and high in sensitivity, the lowest DNA concentration of real-time fluorescence PCR detection can reach the pg level, and the method better meets the actual requirements of eDNA field detection in the upstream of the Yangtze River.
Owner:SOUTHWEST UNIV
Method for double-target digital detection of environmental DNA (Deoxyribonucleic Acid) of Yangtze River cowfish and application
ActiveCN121450816AMicrobiological testing/measurementDNA/RNA fragmentationFinless porpoiseMicrobiology
The invention belongs to the technical field of biological detection, and particularly discloses a method for double-target digital detection of environmental DNA (Deoxyribonucleic Acid) of Yangtze River cowfish and application. According to the method, two pairs of specific primer probes are designed, synchronous identification of two target spots and accurate quantification of the copy number in a single reaction system are achieved, compared with a single target, the environment DNA detection rate of the Yangtze River cowfish is increased, and the method has extremely high species specificity, sensitivity and accuracy. Technical support and theoretical basis are provided for investigating the distribution situation of the field Yangtze River cowfish, and the method is of great significance to protection work of the Yangtze River cowfish. The method is strong in specificity and high in detection sensitivity, and can realize accurate capture of trace environmental DNA of the Yangtze globefish. Experimental data show that the lowest detection limit of the method can reach 0.2 copies / [mu] L, the detection rate under the concentration is 100%, and even under the extremely low concentration of 0.1 copies / [mu] L, the detection rate is still not lower than 80%.
Owner:INST OF AQUATIC LIFE ACAD SINICA
A method for monitoring and early warning of red tide algae based on environmental DNA
Owner:DALIAN OCEAN UNIV
Environmental DNA species monitoring method based on Chinese sturgeons
PendingCN120967002AClimate change adaptationMicrobiological testing/measurementBiotechnologyMicrobiology
The invention discloses an environmental DNA species monitoring method based on Chinese sturgeons, and relates to the technical field of biological gene detection.According to the environmental DNA species monitoring method based on the Chinese sturgeons, mitochondrial whole genomes of the Chinese sturgeons are determined through a method combining a second-generation library-building sequencing technology and a third-generation library-building sequencing technology, and the whole genomes of the mitochondria of the Chinese sturgeons are determined through probe design and PCR amplification sequencing. The sequence of the whole mitochondrial genome of the Chinese sturgeons is combined for comparison, and the specificity difference between the Chinese sturgeons and other related sturgeons is distinguished, so that the efficiency of detecting rare and endangered species Chinese sturgeons by an environmental DNA method is improved, and the accuracy of species identification is improved.
Owner:WUHAN UNIV
Primer, kit and method for identifying species of sparrow
PendingCN120683270AMicrobiological testing/measurementDNA/RNA fragmentationEcological environmentDynamic monitoring
The invention discloses a primer, a kit and a method for identifying species of a sparrow. The primer comprises a primer SEQ ID NO.1 and a primer SEQ ID NO.2 of a targeted COI region, a primer SEQ ID NO.1 and a primer SEQ ID NO.2 of a targeted COI region, and a primer SEQ ID NO.2 of a targeted COI region, the primers of the target D-loop region are shown in SEQ ID NO.3 and SEQ ID NO.4. The invention also discloses a kit for detecting the target D The invention also provides a corresponding kit and a method for identifying the species of the sparrow by using the primer. According to the specific PCR primer for the sparrow, a specific gene sequence of a targeted species is adopted, and accurate detection of DNA of the sparrow can be stably realized in a complex environment medium. The method has the advantages of being easy and convenient to operate, high in sensitivity, rapid in detection and the like, is suitable for scenes such as species identification and environmental DNA monitoring, provides a reliable technical means for ecological research, species protection and dynamic monitoring of the ecological environment of the sparrow, and has wide application prospects and practical values.
Owner:HAINAN NORMAL UNIV +1
Probe primer and method for monitoring holothuria scabra based on environmental DNA technology
The application discloses a kind of probe primer and method based on environmental DNA technology to the probe of rough sea cucumber monitoring.The sequence of the primer is: Hs-F: 5'-GGAGTAAACTTAACCTTCTTC-3', Hs-R: 5'-GTCTGGGTAGTCTGAATAC-3';The sequence of the probe is: Hs-P: 5'-FAM-ATC(C)CC(G)CT(A)AG(C)CTA-BHQ1-3'.The method of the application determines the copy number of rough sea cucumber in water body under different sea areas environment, compares it with the standard curve of corresponding rough sea cucumber DNA copy number and Ct value and detection lower limit LOD, provides the existence condition of field rough sea cucumber, realizes the purpose of monitoring rough sea cucumber, and provides technical support for field distribution investigation of rough sea cucumber and relevant policy making.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI
Primer probe group and method for monitoring distribution of spotted seal based on environmental DNA (Deoxyribose Nucleic Acid)
The invention discloses a primer probe group and a method for monitoring distribution of spotted seal based on environmental DNA (Deoxyribonucleic Acid), and belongs to the technical field of molecular biology and environmental monitoring. The invention relates to a primer probe group for distribution monitoring of spotted seal based on environmental DNA (deoxyribonucleic acid). The primer probe group comprises a primer P-lar-ND2-F with a sequence as shown in SEQ ID No. 1, a primer P-lar-ND2-R with a sequence as shown in SEQ ID No. 2, and a probe P-lar-ND2-P with a sequence as shown in SEQ ID No. 3. The primer probe group comprises a primer P-lar-ND2-F, a primer P-lar-ND2-R, a primer P-lar-ND2-R, a primer P-lar-ND2-R and a probe P-lar-ND2-P, the method is applied to the aspect of distribution monitoring of the spotted seal, and has the characteristics of high specificity and high sensitivity.
Owner:FIRST INSTITUTE OF OCEANOGRAPHY MNR +1
Identification method for comparing cross-habitat living microorganisms based on environment DNA technology
The invention discloses an identification method for comparing cross-habitat living microorganisms based on an environmental DNA technology, and relates to the technical field of environmental microorganisms and molecular biology. According to the method, through an effective pretreatment method, microorganisms are collected through a filter membrane with the pore size of 0.22 mu m and then subjected to PMA dyeing treatment, the comparability of living microorganisms in the water-soil-sediment habitat is enhanced, and the quality of the extracted DNA meets the requirements of high-throughput sequencing and metagenome sequencing; the research on diversity and ecological functions of living microbiome in the environment, especially cross-habitat propagation and ecological health risk assessment of living pathogenic microorganisms, is assisted. The identification method disclosed by the invention has the advantages of unique features, high efficiency, strong operability, simple and conventional required equipment and great potential in the aspects of large-scale popularization and application, and is expected to become an optimal scheme widely applied to related fields.
Owner:INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
DNA collection and analysis method suitable for coral reef area multi-source environment
The invention discloses a coral reef area multi-source environment DNA collection and analysis method. The method comprises the following steps: 1) collecting environmental samples from different sources in a coral reef area, wherein the environmental samples comprise surface water, near-bottom water, a large water body, pore water, sediments and the like; the method comprises the following steps: adding internal reference DNA (danio rerio DNA) into an environmental sample, establishing a positive control sample containing the internal reference DNA (danio rerio DNA) and external reference DNA (oryzias latipes DNA), and extracting DNA in the environmental sample, the positive control sample and the negative control sample by using an improved environmental DNA extraction method; and 2) performing homogenization treatment on data generated by high-throughput sequencing of the DNA sample extracted in the step 1) to realize parallel comparison or combined analysis of different samples. Through parallel comparison and combined analysis of environment DNAs from different sources, species diversity and distribution conditions of coral reefs are reflected more comprehensively.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI
An environmental DNA-based rare and endangered aquatic organism monitoring and alarming device
This invention relates to the field of biodiversity monitoring technology and discloses a monitoring and alarm device for rare and endangered aquatic organisms based on environmental DNA. The device includes a sampling and enrichment unit, a nucleic acid in-situ extraction and purification unit, a isothermal fluorescence detection unit, an environmental factor monitoring unit, a main control and data transmission unit, a power supply and protection unit, and a remote alarm and control platform. The main control and data transmission unit obtains the pressure change rate based on the digital pressure signal output by the microcomputer-controlled pressure sensor, extracts the high-precision peristaltic pump operating time parameter to calculate the actual filtration volume, and performs a division operation to obtain the environmental DNA calibration concentration. It then calculates the dynamic transmission power value by combining the network signal strength indicator value and transmits the complete communication data packet. The remote alarm and control platform obtains a risk index based on the environmental DNA calibration concentration, maps the spatial coordinate signal to an electronic map module to generate coordinate nodes, and displays the risk index and digital environmental parameters in association.
Owner:YUNNAN ACAD OF ENVIRONMENTAL SCI
A method for detecting living eukaryotic plankton in lakes and reservoirs based on environmental DNA technology and its application
The present invention discloses a method for detecting living eukaryotic plankton in lakes and reservoirs based on environmental DNA technology and its application, relating to the fields of biology, ecology, and environmental science and technology. The method comprises the following steps: S1, determining a sampling site; S2, collecting water samples; S3, filtering the samples; S4, PMA treatment; and S5, storing the samples. The present invention utilizes PMA staining during the eukaryotic plankton sample collection process to obtain information about living eukaryotic plankton in the environment. It is capable of detecting and distinguishing the species and number of both total and living eukaryotic plankton, improving detection efficiency, and thereby rapidly and accurately quantifying the diversity of plankton communities of different particle sizes in different water layers of a water body.
Owner:INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
Intelligent diagnosis method for estuary habitat degradation
The present application relates to the technical field of intelligent diagnosis, in particular to a kind of estuary habitat degradation intelligent diagnosis method, comprising S1: obtaining the in-situ time series monitoring data of estuary key section;S2: extract tidal characteristic phase, carry out tidal phase normalization processing, generate standard ecological dataset;S3: standard ecological dataset and the biodiversity index obtained based on environmental DNA technology are spatiotemporal fusion;The response relationship between the ecological parameters in different tidal characteristic phase interval and biodiversity index is modeled, and the response lag feature and coupling strength of each ecological parameter are extracted;Multi-dimensional local dynamic baseline is constructed;S4: time lag compensation is carried out to each dimension index;Based on the index value after time lag compensation, the standardization deviation degree of each dimension index is calculated, and the comprehensive deviation index is obtained by weighted integration;S5: identify the dominant type leading to habitat degradation.The present application improves the accuracy and efficiency of ecological monitoring and management.
Owner:FIRST INSTITUTE OF OCEANOGRAPHY MNR
Fish community monitoring method based on environmental DNA and application thereof
The invention discloses a fish community monitoring method based on environmental DNA and application thereof, and belongs to the technical field of aquatic ecology monitoring, the monitoring method comprises the following specific steps: I, constructing a three-dimensional hydrological model, identifying fish inhabiting hot spots, dynamically setting sampling points, and collecting water samples at the set sampling points; iI, processing the water sample collected at each sampling point, extracting regional fish environment DNA data, and storing each extracted DNA data; iII, collecting eDNA genes in the DNA data, and performing gene amplification and high-throughput sequencing on the eDNA genes to obtain high-throughput sequencing data; according to the method, direct fishing and interference on fish individuals can be avoided, the data acquisition efficiency is improved, the time-space continuity and accuracy of monitoring results are ensured, the sampling representativeness is improved, the eDNA preservation time is prolonged, the degradation risk is reduced, and the accuracy and reliability of species identification are improved.
Owner:TIBET AUTONOMOUS REGION INST OF PLATEAU BIOLOGY
A collection device for monitoring fish community based on environmental DNA technology
ActiveCN120404239BControl the ups and downsEasy to sampleWaterborne vesselsWithdrawing sample devicesFisheryPropeller
The present application relates to the technical field of robots, and especially relates to a collection device for monitoring fish community based on environmental DNA technology, which comprises a sampling device and a sample collecting device, liquid samples are sucked through the sampling device, the liquid samples are stored in the sample collecting device after being delivered, the floating and sinking state of the collection device is controlled through the floating device, so that the collection device is suspended in water, the expansion state of the collection device is controlled through the shape adjusting device, the suspension height of the collection device in water is adjusted, and the screw propeller assembly is arranged on both sides of the shape adjusting device, the movement of the collection device in water is controlled through the cooperation of the screw propeller assembly and the shape adjusting device. The device can make the whole collection device float on the water surface, sample the water area at the surface shallow position of the water body, and also control the floating and sinking state of the whole collection device, so that the collection device is suspended at different heights in water, sampling in multiple positions and multiple aspects is facilitated, and the diversity of sampling is increased.
Owner:TIBET AUTONOMOUS REGION INST OF PLATEAU BIOLOGY