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166 results about "Environmental DNA" patented technology
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Environmental DNA or eDNA is DNA that is collected from a variety of environmental samples such as soil, seawater, snow or even air rather than directly sampled from an individual organism. As various organisms interact with the environment, DNA is expelled and accumulates in their surroundings. Example sources of eDNA include, but are not limited to, feces, mucus, gametes, shed skin, carcasses and hair. Such samples can be analyzed by high-throughput DNA sequencing methods, known as metagenomics, metabarcoding, and single-species detection, for rapid measurement and monitoring of biodiversity. In order to better differentiate between organisms within a sample, DNA metabarcoding is used in which the sample is analyzed and uses previously studied DNA libraries to determine what organisms are present (e.g. BLAST). The analysis of eDNA has great potential, not only for monitoring common species, but to genetically detect and identify other extant species that could influence conservation efforts. This method allows for biomonitoring without requiring collection of the living organism, creating the ability to study organisms that are invasive, elusive, or endangered without introducing anthropogenic stress on the organism. Access to this genetic information makes a critical contribution to the understanding of population size, species distribution, and population dynamics for species not well documented. The integrity of eDNA samples is dependent upon its preservation within the environment. Soil, permafrost, freshwater and seawater are well-studied macro environments from which eDNA samples have been extracted, each of which include many more conditioned subenvironments. Because of its versatility, eDNA is applied in many subenvironments such as freshwater sampling, seawater sampling, terrestrial soil sampling (tundra permafrost), aquatic soil sampling (river, lake, pond, and ocean sediment), or other environments where normal sampling procedures can become problematic.
Power transmission line forest fire risk assessment and early warning system and method
The invention relates to a power transmission line forest fire risk assessment early warning system and method, and belongs to the technical field of power transmission line risk assessment. The system comprises an environment DNA power transmission line forest fire risk assessment and early warning system, a quantum sensing power transmission line fire remote monitoring system, a power transmission line forest fire early warning emergency training system, a digital twin power transmission line fire risk dynamic management system, and a multi-node power transmission line fire early warning system of an edge AI and a low orbit satellite. The system comprises a thermal imaging and deep learning integrated power transmission line fire risk distribution prediction system, an AI-driven power load dynamic adjustment and fire prevention system, an intelligent fire-fighting equipment linked power transmission line fire spreading path prediction system and a distributed quantum computing power transmission line fire emergency simulation and optimization response system. According to the method, the inflammable vegetation density and biocenosis change can be dynamically analyzed through a plant and microorganism gene sequencing technology.
Owner:DANDONG ELECTRIC POWER SUPPLY COMPANY OF STATE GRID LIAONING ELECTRIC POWER SUPPLY +2
Marine biological diversity protection method based on ecological dynamic model
PendingCN120217450AFinanceDatabase distribution/replicationEnvironmental resource managementEcological data
The invention relates to the technical field of marine organism protection, in particular to a marine organism diversity protection method based on an ecological dynamic model, which comprises the following steps: collecting biological and non-biological data in a marine ecosystem, extracting DNA from a seawater sample by utilizing an environmental DNA technology, and constructing the ecological dynamic model based on the collected ecological data and eDNA analysis result. According to the method, the ecological dynamics model is established, the collected data is stored and verified through the block chain technology, and a marine biological diversity protection scheme is designed and implemented according to the ecological dynamics model prediction result and the block chain verification data. Scientific support is provided for protection decision making, protection measures and management regulations are automatically executed through the intelligent contract technology, the system is allowed to respond immediately when it is detected that key environment variables or biological indexes change, and the flexibility and timeliness of responding to environment changes are greatly improved.
Owner:FIRST INSTITUTE OF OCEANOGRAPHY MNR
Enhancement and release seedling resource class evaluation method based on environmental DNA polymerization analysis
PendingCN121331216AData processing applicationsMicrobiological testing/measurementDNA extractionHigh throughput sequence
The invention discloses a method for evaluating enhancement and release seedling resources based on environmental DNA polymerization analysis. The method comprises the following steps: carrying out gridding partition on a target water area, collecting a water sample through a designed sampling scheme, and carrying out DNA extraction and high-throughput sequencing to obtain species sequence information of each sampling point. Sequencing data is subjected to species identification by using a bioinformatics method, released species are identified, a spatial abundance model is established, and a preliminary distribution map is generated. And establishing a DNA degradation kinetic model in combination with water area environmental parameters, and carrying out reverse correction on abundance distribution. And through a resource inversion model coupled with hydrodynamics, analyzing biomass distribution characteristics and migration laws of the release group, and obtaining a resource evaluation result. And finally, a species environment preference model is constructed based on migration path analysis, an optimal release area is matched in a target water area, a scientific scheme including release point locations, opportunities and quantity is generated, and a whole-process technical support and a decision basis are provided for enhancement and release.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
A digital analysis method for tracing water pollution based on off-site monitoring
PendingCN119780371AGeneral water supply conservationMicrobiological testing/measurementEnvironmental resource managementDigital analysis
The present invention discloses a digital analysis method based on off-site monitoring of water pollution source tracing, comprising the following steps: S1, collecting water samples: collecting water samples at multiple predetermined sampling points in the target water area, S2, environmental DNA extraction: performing environmental DNA extraction operation on the collected water samples, S3, microbial gene detection: performing microbial gene detection on the extracted environmental DNA using high-throughput sequencing technology, S4, data analysis and model construction: performing data analysis based on multiple algorithms, S5, pollution source tracing determination: combining satellite remote sensing data, the water color, area changes, algae distribution, floating objects on the water surface and other information of the water area can be monitored.
Owner:南通普利生物科技有限公司
Pelteobagrus vachelli population dynamic monitoring system and method
The invention discloses a Pelteobagrus vachelli population dynamic monitoring system and method, particularly relates to the technical field of fishery resource monitoring, and aims to solve the problems of inaccurate juvenile fish survival rate evaluation and poor monitoring timeliness in a benthic hidden environment in the prior art. The method comprises the following steps: arranging a benthic penetration type sonar array to synchronously collect a water body reflection signal and a benthic environment sample, and extracting juvenile fish specific gene fragments by combining an environment DNA (Deoxyribose Nucleic Acid) enrichment technology to generate concentration distribution data; screening juvenile fish distribution data by using an acoustic reflection signal and body length correlation model, and positioning an active hot spot area through space-time overlapping analysis; deploying a directional sound wave excitation device to obtain escape trajectory data, and calculating a survival rate in combination with a density attenuation model; finally, multi-source data and topographic features are fused to generate a real-time population dynamic monitoring result, and high-precision and non-intrusive monitoring of the juvenile fish population in the hidden environment is realized.
Owner:FISHERIES RES INST ANHUI ACAD OF AGRI SCI
A method for predicting river aquatic biodiversity based on remote sensing and environmental DNA
The present invention discloses a method for predicting river aquatic biodiversity based on remote sensing and environmental DNA. The method comprises the following steps: determining the abundance of a first species at each sampling point in the entire river basin based on the environmental DNA of each sampling point; determining the patch corresponding to each sampling point based on the sampling point and the species activity range, and determining the first remote sensing index based on the pre-acquired remote sensing vegetation index and the corresponding first species abundance in the patch; training a first preset model based on the first remote sensing index and the corresponding first species abundance in each patch, and determining the first preset model as a species abundance model when the first preset model reaches a preset standard; dividing the entire river basin into multiple patches, and determining a second remote sensing index in each patch, wherein the second remote sensing index is determined by the remote sensing vegetation index corresponding to the first remote sensing index; inputting the second remote sensing index in each patch into the species abundance model, and determining the second species abundance in the entire river basin, so as to determine the species diversity in the entire river basin.
Owner:GUANGDONG UNIV OF TECH
Water environmental DNA collection apparatus
A water environmental DNA collection apparatus, including a lower box body and collection components, where a clear water chamber, a motor chamber, a water pump chamber and a power supply chamber are provided in the lower box body, a motor is provided in the motor chamber, a water pump is provided in the water pump chamber, the lower box body is fixedly connected with a first filtering mechanism, the water pump communicates with the first filtering mechanism, a support column is fixedly connected outside the lower box body, and a turntable is fixedly connected with the support column; and a plurality of groups of collection components are provided, each collection component includes a press filter, a cylinder and a bottom shell, a transverse plate is fixedly connected inside the cylinder, the cylinder is divided into a filter pressing chamber and a filtering chamber through the transverse plate.
Owner:CHINESE RES ACAD OF ENVIRONMENTAL SCI
Remote autonomous environmental DNA sampler and analyzer
Systems and methods for operating an aquatic robot. The methods comprise: autonomously propelling the aquatic robot through a body of water to a location where a water sample is to be obtained; and performing operations by the aquatic robot to autonomously collect the water sample, cause the water sample to flow through a filter that retains eDNA, lyses and releases the eDNA to a create a lysate, process the lysate to obtain a product for eDNA sequencing, generate eDNA sequencing data using the product, and communicate the eDNA sequencing data to a remote external device.
Owner:UT BATTELLE LLC
Water quality evaluation method based on data analysis
PendingCN119624228Aaccurate analysisimprove accuracyEnsemble learningGeneral water supply conservationParticulatesOrganism
The invention relates to the technical field of water quality analysis, in particular to a water quality evaluation method based on data analysis, which comprises the following steps: based on a satellite remote sensing technology, analyzing water spectral characteristics by adopting a deep neural network algorithm, and generating water characteristic data. According to the method, the satellite remote sensing technology and the deep neural network are combined, the accuracy of water body spectral feature analysis is improved, particulate matter in water is classified through image recognition, the analysis speed is increased, the result accuracy is improved, environmental DNA sampling is used for evaluating the biological diversity, and basic data are generated for microbial activity analysis; a micro-fluidic chip and a fluorescence labeling method are used for improving the activity analysis sensitivity of microorganisms and algae, a support vector machine algorithm is used for improving the water quality detection accuracy, an autoregression integral moving average model and a random forest algorithm are combined for deep water quality change analysis, and a comprehensive risk assessment model is used for comprehensively assessing the water quality risk. And a basis is provided for accurate water quality management.
Owner:CHINA GEOLOGICAL SURVEY XINING NATURAL RESOURCES COMPREHENSIVE SURVEY CENT
Real-time certification and lifecycle automation of environmental credits
A system for real-time certification and lifecycle automation of environmental credits. The system comprises: (i) a physical data collection layer equipped with on-land sensors, aerial LiDAR, satellite imagery, environmental DNA (eDNA) sampling, and community-contributed field data; (ii) an Ecological Oracle Engine, which uses an AI protocol to validate, classify, and interpret multi-source ecological data, supported by an adaptive reference library; and (iii) a smart contract protocol deployed on a blockchain network, configured to autonomously convert verified ecological data into financial instruments by issuing, pricing, transferring, freezing, and retiring tokenized environmental credits. The system performs continuous verification of each credit and includes freezing functionality based on ecological performance. It supports multiple credit types, including but not limited to carbon, biodiversity, and water credits. The system may be integrated with external modules for integrates with a Digital Twin of Nature and Web3 ecosystems, including decentralized marketplaces, ReFi platforms, and DAO mechanisms.
Owner:SYTNYK OLHA
Method for detecting apple snails
PendingJP2025151531ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyPomacea diffusa
To detect apple snails from environmental DNA with a high detection rate.SOLUTION: This method amplifies and detects environmental DNA in a sample with a first primer having a specific base sequence, a second primer having another specific base sequence, and a probe having still another base sequence.SELECTED DRAWING: None
Owner:NAT AGRI & FOOD RES ORG
Probe method-based kit and method for tracking behavior trajectory of acipenser dabryanus dumeril released upstream of yangtze river
PCT designated stage expiredWO2025077329A1Microbiological testing/measurementDNA/RNA fragmentationFluoProbesZoology
Disclosed are a probe method-based kit and method for tracking a behavior trajectory of Acipenser dabryanus Dumeril released upstream of the Yangtze River. By analyzing mitochondrial DNA sequences of Acipenser dabryanus Dumeril and common fish species in the Yangtze River basin, a pair of specific primers ND5-F4 / ND5-R4 capable of specifically amplifying mitochondrial DNAs of Acipenser dabryanus Dumeril and a Taqman fluorescent probe ND5 Probe 4 are designed and screened. A kit composed of the specific primers, the Taqman fluorescent probe, and a ddPCR master mix is used to carry out ddPCR testing on environmental DNA samples collected before and after release, so that a behavior trajectory of the released Acipenser dabryanus Dumeril can be tracked and monitored, achieving "non-contact" tracking of the behavior trajectory of the released Acipenser dabryanus Dumeril, avoiding damage to fish bodies in the tracking process, leading to reduced tracking costs and improved accuracy of overall tracking.
Owner:CHINESE STURGEON RES INST OF CTG
Microbial indicator species screening method supporting water resource scheduling decision
The invention relates to a microbial indicator species screening method for supporting a water resource scheduling decision, which is used for screening representative microbial indicators by combining an environmental DNA (eDNA) technology and monitoring the dynamic change of the microbial indicators so as to monitor and evaluate the diffusion range and process of the indicators and provide comprehensive support for the water resource scheduling decision. The blank of related methods is filled, and the ecological benefit, the environmental benefit and the economic benefit of water resource scheduling are comprehensively improved.
Owner:TAIHU BASIN HYDROLOGY & WATER RESOURCES MONITORING CENT (TAIHU BASIN WATER ENVIRONMENT MONITORING CENT) +1
Phytoplankton spatial distribution prediction method based on environmental DNA and machine learning
PendingCN120354761AEnsemble learningMicrobiological testing/measurementEcological environmentData set
The invention discloses a phytoplankton space distribution prediction method based on environment DNA and machine learning, and belongs to the technical field of ecological environment evaluation and treatment. Comprising the following steps: determining a sampling site, and collecting a phytoplankton eDNA sample; carrying out DNA extraction and treatment on the collected eDNA sample, and calculating a species abundance matrix; screening a training data set for training a prediction model according to the sampling time and the sampling site of the eDNA sample; inputting the training data set as an independent variable of a training set, inputting the species abundance matrix as a dependent variable of the training set, and training a prediction model; and inputting remote sensing image data into the prediction model to obtain a species spatial distribution diagram. Compared with the prior art, the method has the advantages that efficient and high-species-resolution spatial distribution prediction of algae species such as blue-green algae can be achieved, the phytoplankton prediction model is trained through the machine learning model, automatic operation of spatial prediction of various phytoplanktons is achieved, the arrangement density of sampling points is reduced, and the monitoring efficiency is improved.
Owner:YUNNAN UNIV +2
Rapid sampling device for environment DNA (deoxyribonucleic acid)
ActiveCN222529022UQuick disassembly for cleaningWithdrawing sample devicesStationary filtering element filtersSuction forceEnvironmental engineering
The utility model discloses an environmental DNA quick sampling device, which relates to the technical field of water body environmental DNA sampling, and comprises a sampling box, one side of the surface of the sampling box is fixedly connected with a support plate, the top surface of the support plate is fixedly connected with a water suction pump, the water outlet end of the water suction pump is fixedly connected with one side of the sampling box, and the water outlet end of the water suction pump is fixedly connected with the other side of the sampling box. The water inlet end of the water suction pump is fixedly connected with a water inlet pipe, and one end of the water inlet pipe is provided with a filtering assembly which is used for filtering impurities in a water body when the water suction pump pumps samples so as to prevent the impurities from blocking the water suction pump. Suction force is provided through the water suction pump, then the sampling assembly is used for sampling a water body, and the sampled water body is filtered through the filtering assembly to block impurities in the water, so that the problem that the impurities in the water enter the water suction pump during sampling, the water pump is blocked, and the sampling speed of the water suction pump is influenced is solved; meanwhile, the filtering assembly can be quickly disassembled and cleaned by utilizing the limiting assembly and the auxiliary assembly.
Owner:南通普利生物科技有限公司
Primer and method for quantitatively monitoring biomass of sargassum hemiphyllum based on environmental DNA (Deoxyribose Nucleic Acid) technology
ActiveCN121737340AMicrobiological testing/measurementDNA/RNA fragmentationSargassum hemiphyllumDeoxyribose
The invention discloses a primer and a method for quantitatively monitoring the biomass of sargassum hemiphyllum based on an environmental DNA technology, and belongs to the technical field of molecular ecology. The primer probe group comprises an upstream primer, a downstream primer and a fluorescent probe which are specifically targeted to the sargassum hemiphyllum mitochondria COX1 gene, and the sequence is shown as SEQ ID NO.1-3. The kit comprises the primer probe group, a qPCR (quantitative polymerase chain reaction) premixed solution, nuclease-free water and a sargassum hemiphyllum plasmid positive control. The method comprises the following steps: collecting a water sample, enriching eDNA, performing qPCR detection by using the primer probe group after extraction and purification, and realizing qualitative detection and quantitative evaluation of sargassum hemiphyllum through a Cq value or a standard curve. The method disclosed by the invention has the advantages of high sensitivity, strong specificity, no damage to the environment and target organisms, capability of realizing large-scale rapid general survey and the like, and is suitable for early warning of gulfweed blooms, investigation of population distribution and evaluation of ecological influence.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI
Minimum population wild plant identification and early warning method and system
The invention discloses a minimum population wild plant identification and early warning method and system. The system comprises: an environmental DNA monitoring network unit, which analyzes environmental DNA through automatic sampling, evaluates the state of species and triggers a first early warning; the biological voiceprint monitoring and recognition unit is used for monitoring related acoustic activities and illegal intrusion behaviors of the target species by using intelligent recording nodes deployed in a gridding manner and a voiceprint recognition model, and triggering second early warning; and the multi-source information fusion and causal inference unit is used for integrating the environment DNA, the voiceprint and the meteorological data, analyzing the causal relationship among the ecological factors based on a causal inference model, generating protection measures and outputting comprehensive early warning. According to the invention, all-weather, interference-free and precise monitoring of the extremely small population wild plants is realized, the problems of low efficiency, many blind areas, large interference and the like of a traditional monitoring mode are solved, and effective technical support is provided for protection of rare plants.
Owner:陕西省林业科学院
A comprehensive evaluation method and system for multi-source data management and correction of fish resources in watersheds.
This application discloses a comprehensive assessment method and system for multi-source data management and correction of watershed fish resources. The method includes: extracting multi-dimensional features from a first data matrix to obtain a first feature matrix; classifying species data based on anomaly scores, outputting verification results, and correcting the first feature matrix to obtain a second feature matrix; acquiring environmental DNA sequencing data and corresponding environmental data; obtaining a first eDNA dataset from the environmental DNA sequencing data through ecological screening; correcting the species in the first eDNA dataset to obtain a second eDNA dataset; fusing the second eDNA dataset with the second feature matrix; calculating biodiversity indices and analyzing community structure; analyzing long-term trends in population and community structure; and generating a comprehensive assessment report. This application achieves full-process automation and intelligence from data quality control and fusion to assessment, significantly improving the accuracy and efficiency of watershed fish resource assessment.
Owner:WUHAN UNIV
Marine benthic ecological environment quality assessment method based on environment DNA
PendingCN120249455AMicrobiological testing/measurementBiostatisticsEnvironmental resource managementEcological environment
The invention belongs to the field of ecological environment evaluation and treatment, and particularly relates to a marine benthic ecological environment quality evaluation method based on an environmental DNA technology. Comprising the following steps: 1) collecting an environmental DNA sediment sample; 2) extracting sediment environment DNA from the environment DNA sediment sample; (3) amplifying macro bar code fragments by taking the environmental DNA extracted in the step (2) as a template, and sequencing; (4) analyzing the species category of the sequencing result of the macro bar code in the step (3), and defining the macrobenthonic animals; and 5) evaluating the quality of the marine benthic ecological environment by combining the environment DNA macro bar code with AMBI. The environment DNA macro bar code technology is applied to marine benthonic habitat quality evaluation, and environment DNA PCR amplification, screening of large benthonic animals in sequence species, AMBI index data calculation and habitat quality evaluation grade technologies are defined; wherein screening of large benthonic animals in an environment DNA macro bar code sequence and introduction of sequence absolute abundance into AMBI calculation are key technologies for influencing a final marine benthonic habitat quality evaluation result.
Owner:BEIHAI FORECASTING CENT OF STATE OCEANIC ADMINISTRATION ((QINGDAO MARINE FORECASTING STATION OF STATE OCEANIC ADMINISTRATION) (QINGDAO MARINE ENVIRONMENT MONITORING CENT OF STATE OCEANIC ADMINISTRATION))
Environmental DNA extraction kit based on magnetic nanoparticles as well as extraction method and application of environmental DNA extraction kit
The invention discloses an environmental DNA (deoxyribonucleic acid) extraction kit based on magnetic nanoparticles as well as an extraction method and application of the environmental DNA extraction kit. The environment DNA extraction kit comprises a nanoparticle solution, pH test paper, a Tris solution, nuclease-free water and diluted hydrochloric acid. By using the extraction kit, enrichment and extraction are carried out according to the designed magnetic nanoparticle dosage and eluent volume, the environmental DNA in the water body can be enriched and extracted, the process steps are simple and convenient, the required field sampling equipment is less, the use of toxic organic reagents is avoided, meanwhile, the method also has the advantage of easy realization of automation, eDNA can be obtained from the water body on a large scale, and the method is suitable for industrial production. And the extraction effect is good. The quality and purity of the eDNA extracted by the method are superior to those of the eDNA extracted by the traditional method, the extraction effect is good, and reliable theoretical and technical bases are provided for optimization of an environmental DNA technology and establishment of a unified standard.
Owner:OCEAN UNIV OF CHINA
Filter assembly, kit and methods
The present invention is directed to a filter assembly for capturing environmental DNA (eDNA), a kit comprising the filter assembly, a method of capturing eDNA using the filter assembly, a method of analysing eDNA captured in the filter assembly, and a method of providing biodiversity data by analysing eDNA collected in the filter assembly.
Owner:NATURE METRICS LTD
An environmental DNA-based primer probe combination for oncorhynchus masonii and application thereof
The application discloses a primer probe combination for Salmo platycephalus based on environmental DNA and application thereof, and belongs to the technical field of molecular biology and endangered aquatic organism monitoring. The application provides the primer probe combination for Salmo platycephalus, which comprises upstream and downstream primers and a probe P, and the amplified fragment has a length of 179 bp; the primer probe combination for Salmo platycephalus can specifically detect Salmo platycephalus in environmental DNA, can efficiently monitor and protect Salmo platycephalus, and can effectively avoid the interference of related species including Salmo, Oncorhynchus and Leucichthys bampsii; the provided primer probe combination for Salmo platycephalus can be applied to the monitoring of Salmo platycephalus in different water bodies to be detected such as streams, rivers and reservoirs, is easy to popularize, and has important significance for the protection and monitoring of Salmo platycephalus.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Method and system for comprehensive water quality assessment by integrating biotic and abiotic factors
Provided is a method and system for comprehensive water quality assessment by integrating biotic and abiotic factors. The method includes: acquiring abiotic factors of a water body to be tested; constructing a biotic factor indicator library by an environmental DNA technology; determining a biotic-abiotic response relationship-based abiotic factor weight matrix using the abiotic factors and the biotic factor indicator library; acquiring a machine learning-based abiotic factor weight matrix using the abiotic factors and a LightGBM model; determining an abiotic factor comprehensive weight matrix according to the biotic-abiotic response relationship-based abiotic factor weight matrix and the machine learning-based abiotic factor weight matrix; and conducting the comprehensive water quality assessment of the water body to be tested based on the abiotic factor comprehensive weight matrix and the abiotic factors to determine a comprehensive water quality assessment result of the water body to be tested.
Owner:PEKING UNIV
Homogenizing mechanism applicable to environmental DNA enricher
ActiveCN222882408UBioreactor/fermenter combinationsBiological substance pretreatmentsBiologyMechanical engineering
The utility model relates to the technical field of environmental monitoring, in particular to a homogenizing mechanism applicable to environmental DNA enrichers, which comprises an adapter used for being connected with a vortex grinding device and also used for placing a plurality of environmental DNA enrichers; the gland is detachably connected with the adapter, and the gland is used for fixing a plurality of environmental DNA enrichers placed on the adapter. A plurality of environmental DNA enrichers can be installed through the adapter, the enrichers can be pressed and fixed through the gland, and the homogenizing mechanism and the vortex grinding device can act synchronously after being connected, so that the homogenizing and grinding functions are realized.
Owner:NANJING YIJINUO ENVIRONMENTAL PROTECTION TECH CO LTD
Primer group for identifying phthorimaea operculella based on ddPCR and method for identifying phthorimaea operculella by detecting environmental DNA
PendingCN120624682AMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyForward primer
The invention discloses a primer group for identifying phthorimaea operculella based on ddPCR and a method for identifying phthorimaea operculella by detecting environmental DNA, and belongs to the technical field of pest detection. The primer group for identifying the phthorimaea operculella based on the ddPCR comprises a forward primer, a reverse primer and a specific fluorescent probe, the nucleic acid sequences of the forward primer, the reverse primer and the specific fluorescent probe are as shown in SEQ ID NO.1-SEQ ID NO.3; the C basic group at the 3'end of the forward primer, the A basic group at the 3 'end of the reverse primer, and the A basic groups and the T basic groups at the 12th site and the 13th site of the specific fluorescent probe are basic groups modified by locked nucleic acid. The primer group disclosed by the invention has very high specificity and sensitivity to the phthorimaea operculella, can be detected by taking eDNA as a template, can find the phthorimaea operculella earlier, and has an important application prospect in early detection and prevention of the phthorimaea operculella.
Owner:QINGDAO AGRI UNIV
Primer, probe and kit for identifying coreius guichenoti based on environmental DNA
The invention provides a primer, a probe and a kit for identifying coreius guichenoti based on environmental DNA, and a specific primer and a probe designed according to a COI gene sequence in coreius guichenoti mtDNA, namely the primer and the probe can specifically identify coreius guichenoti in numerous fishes, so that the detection time is greatly shortened, the detection can be completed within 6 hours, and the detection efficiency is greatly improved. The method is simple in operation, accurate in monitoring result and high in sensitivity, the lowest DNA concentration of real-time fluorescence PCR detection can reach the pg level, and the method better meets the actual requirements of eDNA field detection in the upstream of the Yangtze River.
Owner:SOUTHWEST UNIV
Method for double-target digital detection of environmental DNA (Deoxyribonucleic Acid) of Yangtze River cowfish and application
ActiveCN121450816AMicrobiological testing/measurementDNA/RNA fragmentationFinless porpoiseMicrobiology
The invention belongs to the technical field of biological detection, and particularly discloses a method for double-target digital detection of environmental DNA (Deoxyribonucleic Acid) of Yangtze River cowfish and application. According to the method, two pairs of specific primer probes are designed, synchronous identification of two target spots and accurate quantification of the copy number in a single reaction system are achieved, compared with a single target, the environment DNA detection rate of the Yangtze River cowfish is increased, and the method has extremely high species specificity, sensitivity and accuracy. Technical support and theoretical basis are provided for investigating the distribution situation of the field Yangtze River cowfish, and the method is of great significance to protection work of the Yangtze River cowfish. The method is strong in specificity and high in detection sensitivity, and can realize accurate capture of trace environmental DNA of the Yangtze globefish. Experimental data show that the lowest detection limit of the method can reach 0.2 copies / [mu] L, the detection rate under the concentration is 100%, and even under the extremely low concentration of 0.1 copies / [mu] L, the detection rate is still not lower than 80%.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Primer group and kit for detecting invading sunfish species based on environmental DNA (Deoxyribose Nucleic Acid)
PendingCN119710020AMicrobiological testing/measurementInvasive species monitoringMicrobiologyGel electrophoresis
The invention belongs to the technical field of biology, and particularly discloses a primer combination, a kit and a method for detecting an invasive species sunfish in water by utilizing multiple PCR (Polymerase Chain Reaction). According to the invention, a primer design method is innovated, a dominant sunfish multiple PCR primer combination is screened out, and the sunfish DNA can be detected under the background of various types of fishes in combination with an optimized PCR reaction system and conditions, so that the problem of environmental eDNA detection is solved, multiple PCR primers can be combined with environmental eDNA detection, and the diffusion trend of the sunfish can be monitored more accurately. The detection method can effectively save time and cost, has high sensitivity, strong specificity, high efficiency and low professional requirements, has a good detection rate when the concentration of a target species is low, and can directly obtain a result through gel electrophoresis.
Owner:HONGMA (SHANGHAI) BIOTECHNOLOGY CO LTD +1
A method for monitoring and early warning of red tide algae based on environmental DNA
Owner:DALIAN OCEAN UNIV
Environmental DNA species monitoring method based on Chinese sturgeons
PendingCN120967002AClimate change adaptationMicrobiological testing/measurementBiotechnologyMicrobiology
The invention discloses an environmental DNA species monitoring method based on Chinese sturgeons, and relates to the technical field of biological gene detection.According to the environmental DNA species monitoring method based on the Chinese sturgeons, mitochondrial whole genomes of the Chinese sturgeons are determined through a method combining a second-generation library-building sequencing technology and a third-generation library-building sequencing technology, and the whole genomes of the mitochondria of the Chinese sturgeons are determined through probe design and PCR amplification sequencing. The sequence of the whole mitochondrial genome of the Chinese sturgeons is combined for comparison, and the specificity difference between the Chinese sturgeons and other related sturgeons is distinguished, so that the efficiency of detecting rare and endangered species Chinese sturgeons by an environmental DNA method is improved, and the accuracy of species identification is improved.
Owner:WUHAN UNIV