The invention discloses an
environmental DNA macro bar code method for researching a macrobenthic animal
community structure. One technical scheme to be protected by the invention is the application of the primer pair in detecting the
community structure of the macrobenthos in the
water area to be detected. The primer pair is named as COI and is composed of a primer 1 and a primer 2, and the primer 1 and the primer 2 can be a sequence 1 and a sequence 2 in a sequence table. Compared with a traditional method, the COI primer provided by the invention is used for amplifying an environmental DNAsample of a to-be-detected
water area, and sequencing and
annotation analysis are carried out to obtain more obvious species, especially benthonic
animal groups which are easy to escape, small in sizeand partial in
life history in water; and the method can supplement benthonic animal abundance data which are easy to ignore, difficult to find and small in size in a traditional investigation method, effectively improves the identification level,
species detection rate and abundance evaluation accuracy of the large benthonic animals, and is an effective and reliable new method for investigatingthe
community structure of the large benthonic animals.