100 results about "Ruditapes" patented technology

The invention provides a high-efficient ecological seedling-raising method of blue crabs. The high-efficient ecological seedling-raising method comprises the following steps of: selecting parent crabs after artificial breeding, adopting circulating water to keep the water temperature in an overwintering pond, feeding clam worms, ruditapes philippinarum, squid and other baits for performing intensive culture on the parent crabs, utilizing a single-crab and single-pond mode to perform pond arrangement, and laying disinfection sea mud at the bottom of a larval rearing tank for simulating a sea natural ecological environment. By using the method disclosed by the invention, vertical transmission of diseases can be avoided, the use of coal is reduced, and energy conservation and emission reduction are realized; and the baits can be reasonably matched for promoting the healthy development of embryos, and self-mutilation of larvae can be reduced for improving the yield and the quality of seedlings. According to the method disclosed by the invention, the yield of the seedlings of the blue crabs can be improved by 30-50%, the outdoor farming survival rate can be improved by 10-15%, and the method can be widely applied to offshore sea waters and has significant economic benefits.

The invention relates to a method for realizing ecological larval culture on rapana venosa. The method is characterized by comprising the following steps of: firstly selecting a pond which is convenient in water exchange, takes lithoherm and stone bottom as a substrate and is in the size of 2-5Mu; then placing mature rapana venosa with the length of 7-10cm into the pond in the quantity of 100kg per Mu, mating and spawning after the rapana venosa eats ruditapes and mussel for 2-3 days, when enough oocysts are obtained, removing the rapana venosa away, incubating the oocysts for 20-22 days at the water temperature of 20-25 DEG C to obtain planktonic larva, growing the planktonic larva by eating the planktonic larva in the pond, starting to throwing polyethylene corrugated plate adherence ortile under the condition that the water temperature is 22-25 DEG C after 15-16 days, sticking benthic diatom and the planktonic larva onto the polyethylene corrugated plate or tile, transfigurating the planktonic larva of the rapana venosa into young spiral shell after 5-6 days, growing the transfigurated young spiral shell by eating the planktonic larva and benthic diatom on the corrugated plate, wherein the survival rate of the transfigurated young spiral shell on the corrugated plate can reach more than 50%, and feeding small low-value bivalve to the young spiral shell with the length morethan or equal to 3mm. By applying the method provided by the invention, the defects that the survival rate is low and the larval yield is low as bait conversion is difficult and water is polluted when carnivorous fish mice are used as feed in indoor artificial larval culture can be overcome, and the larval yield is improved by more than 50% compared with the indoor artificial larval culture. Meanwhile, the production cost is saved as no bait is thrown into the pond, thus the method is environment-friendly, and the produced larval is strong.

The invention relates to the technical field of water treatment, in particular to a method for preparation of a heavy metal adsorbent from a marine Psychrobacter strain. The selected marine Psychrobacter strain Psychrobacter sp.GHF2 is separated from the sludge liquid spit by ruditapes philippinarum, the strain is subjected to enlarged culture to prepare a strain solution, and fermentation cultureis carried out to produce abundant exopolysaccharide, then the fermented strain solution is dialyzed to remove part of medium components, and centrifugation is conducted to obtain supernatant, ethanol precipitation purification is carried out twice to obtain a refined extraction product of exopolysaccharide, and the refined extraction product of exopolysaccharide is mixed with agar and starch toprepare the heavy metal adsorbent. The heavy metal adsorbent has high adsorption efficiency, can be used directly in drinking water, and is safe and harmless.

The invention discloses an artificial cultivation method of hemifusus termatamus and relates to shellfish cultivation. The invention provides the artificial cultivation method of hemifusus termatamus, which can effectively reduce cultivation cost and labor intensity, is convenient for daily inspection and has convenient operation and management. The method comprises the following steps: selecting cultivation facilities and water sources; before stocking young hemifusus termatamus, sterilizing, when using a land cultivation pond, laying attaching base at the bottom of the pool for the young hemifusus termatamus to attach, and cleaning the bottom and sucking sewage regularly after stocking the young hemifusus termatamus; after starting to cultivate the young hemifusus termatamus, feeding with bait every day, wherein the bait comprises the following ingredients according to mass ratio: 50-70 of sinonovacula constricta, 30-60 of ruditapes philippinarum, 40-70 of trash fish and 10-30 of laminaria japonica; when cultivating the hemifusus termatamus, cleaning residual bait at the bottom of the pool, on the attaching base or in the cultivation cage every day, when the shell length of the young hemifusus termatamus reaches 1-3cm, the stocking density is 300-600 / m2, when the shell length reaches more than 5cm, carrying out division, and controlling the stocking density of the young hemifusus termatamus to be within 30-60 / m2.

The invention discloses a method for breeding ruditapes philippinarum. The method includes steps of selecting individuals with full gonads as parents in ruditapes philippinarum sexual maturity periods; taking gonad samples of the parents; performing microscope examination on the gonad samples in fresh seawater; selecting first certain individuals as female parents and selecting second certain individuals as male parents; placing acquired egg liquid in fresh seawater for 15-45min; activating the egg liquid; placing sperms in the successfully activated egg liquid, performing fertilization in the successfully activated egg liquid, specifically, placing 3-5 sperms around each egg and performing fertilization in a stirring mode; hatching the eggs in a slight inflation manner; cultivating offspring according to a convention process for cultivating the ruditapes philippinarum to acquire large quantities of juvenile mollusks and adults. The first certain individuals have eggs which are microscopically perfectly circular, the eggs are gray black and have complete egg envelopes, the diameters of the eggs are consistent, the diameters of the eggs of northern colonies of the first certain individuals are 70.0+ / -0.5 micrometers, and the diameters of the eggs of southern colonies of the first certain individuals are 60.0+ / -0.5 micrometers. The second certain individuals have the sperms all of which jump microscopically. The method has the advantages of simplicity and convenience in operation, easiness in popularization and the like.

The invention belongs to the technical field of biology, and particularly relates to a Ruditapes philippinarum species real-time fluorescent PCR (polymerase chain reaction) specific detection system and application thereof. The sequences of the primers and probe are as follows: 5'-ggttgaacagtctaccctcc-3' (forward); 5'-gctaacatactactacgcaaaa-3' (reverse); and 5'-tctcttcacgtaggtggtgtctcttcaatt-3' (probe). After the Ruditapes philippinarum species specific detection primers and probe are adopted to perform real-time fluorescent PCR amplification on CoxI gene of Ruditapes philippinarum, a specific amplification curve can be generated, thereby performing specific identification on the Ruditapes philippinarum components. The specific primers and probe are reasonably designed, and have the advantages of favorable specificity and high detection sensitivity when being used for Ruditapes philippinarum species detection. The fluorescent PCR analysis can be performed to accurately identify the Ruditapes philippinarum components, thereby having favorable specificity.

The invention relates to the field of solen grandis spat rearing, in particular to a method for solen grandis spat rearing. In the aspect of a water pretreatment method for spat rearing, biological secondary purification is conducted on seawater obtained after sand filtration by using ruditapes variegate, toxic and harmful substances in the seawater can be further removed, and a good water quality condition is provided for the production of spat. In the aspect of an oviposition method, an oviposition barrel is used for oviposition, movable operation of the parent shellfish is achieved, and the problems that the oviposition density of the solen grandis is too large and the number of sperms is too large, and accordingly egg membrane dissolution or fertilized egg development malformation is caused can be solved. In the aspect of a fertilized egg hatching method, a method for hatching fertilized eggs in an original oviposition pond is used, so that the problems that the fertilized eggs are damaged due to water flow impact, friction and extrusion in the fertilized egg collection process, the fertilized eggs cannot be completely collected, and waste is caused are solved. In the aspect of selection and application of adhering substrates, the two materials including sea mud and fine sand serve as the adhering substrates in different juvenile shellfish stages, harmful organisms are removed easily, and the growth speed and the survival rate of juvenile shellfishes are increased.

The invention discloses a mixed overwintering farming method of Portunus trituberculatus, Takifugu rubripes and Ruditapes philippinarum and belongs to the field of aquaculture. By making use of differences of ecological niches of species, Portunus trituberculatus, Takifugu rubripes and Ruditapes philippinarum are comprehensively overwintered and farmed in an indoor overwintering pond. It is prevented that Portunus trituberculatus preys on Ruditapes philippinarum; a protective net is used in a Ruditapes philippinarum farming zone, can prevent sand in the farming pond from flowing out during water changing and can also protect Ruditapes philippinarum. Ruditapes philippinarum is farmed in the 20% of the farming pond under the farming density of 100 individuals per m<2>; stocking density of Portunus trituberculatus is 5 individuals per m<2>; stocking density of Takifugu rubripes is 10 individuals per m<2>; when daily quantity of feed which is fresh trash fishes accounts for 5% of the weight of Portunus trituberculatus, ecological balance is exactly achieved for the three species in the farming pond; the feed is made full use, little water is changed, and resources are saved.