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Nucleoside ingredient obtained from ocean low-value shellfish through separation and purification and preparation method and application thereof

A low-value shellfish, separation and purification technology, applied in the field of marine low-value shellfish, can solve the problems of low purity, long production cycle, and cumbersome steps in the separation process, and achieve reasonable process design, short production cycle, and strong operability Effect

Active Publication Date: 2016-10-12
浙江海富海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems in the prior art are: the steps of the separation process are cumbersome, the production cycle is long, the production cost is high, and the extraction agents or eluents such as chloroform used in the separation are poor in environmental protection, and the types of nucleoside substances obtained by separation less, less pure, less valuable

Method used

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  • Nucleoside ingredient obtained from ocean low-value shellfish through separation and purification and preparation method and application thereof
  • Nucleoside ingredient obtained from ocean low-value shellfish through separation and purification and preparation method and application thereof
  • Nucleoside ingredient obtained from ocean low-value shellfish through separation and purification and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for separating and purifying nucleoside components from marine low-value shellfish, comprising the following steps:

[0031] (1) Preparation of the alcohol-precipitated supernatant of four-cornered clam: take fresh four-cornered clam soft body, wash and drain, weigh, add 3 times the amount of water to decoct, heat and reflux twice, each time for 45 minutes, filter, and combine the filtrate , concentrated, centrifuged to remove the precipitate, to obtain the supernatant, add ethanol to make the ethanol concentration 80%, let it stand overnight, and suction filter to obtain the supernatant; the supernatant is concentrated until it has no alcohol smell, and is desalted by electrodialysis with a voltage of 30V , the loading concentration is 30g (solid content) / L, and the desalination time is 1.5h.

[0032] (2) Coarse separation of macroporous adsorption resin column: take 1.6 times column volume of the four-cornered clam soft body water extraction alcohol precipita...

Embodiment 2

[0040] A method for isolating and purifying nucleoside components from clam philippines, comprising the following steps:

[0041] (1) Preparation of the ethanol-precipitated supernatant of Philippine clam: take fresh Philippine clam software, wash and drain, weigh, add 3 times the amount of water, heat and reflux twice, each time for 60 minutes, filter, and combine the filtrate , concentrated, centrifuged to remove the precipitate, to obtain a supernatant, added ethanol to make the ethanol concentration 75%, allowed to stand overnight, and filtered to obtain a supernatant. The supernatant was concentrated until there was no alcohol smell, and desalted by electrodialysis with a voltage of 20V, a sample concentration of 20g (solid content) / L, and a desalting time of 2h.

[0042](2) Coarse separation by macroporous adsorption resin column: take 2 times the column volume of the supernatant of philippine clam software prepared in step (1) by water extraction and ethanol precipitati...

Embodiment 3

[0050] A method for separating and purifying nucleoside components from cockles, comprising the following steps:

[0051] (1) Preparation of ethanol-precipitated supernatant of cockles: take fresh cockles soft body, wash and drain, weigh, add 4 times the amount of water, heat and reflux twice, 50min each time, filter, combine filtrates, concentrate, and centrifuge Remove the precipitate to obtain a supernatant, add ethanol to make the ethanol concentration 80%, let stand overnight, and filter with suction to obtain a supernatant. The supernatant was concentrated until it had no alcohol smell, and was desalted by electrodialysis with a voltage of 30V, a sample concentration of 34g (solid content) / L, and a desalination time of 1.5h.

[0052] (2) Coarse separation by macroporous adsorption resin column: take 1.5 times the column volume of the water-extracted and ethanol-precipitated supernatant of the clam software prepared in step (1), put it on the SP207 type macroporous adsorp...

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Abstract

The invention discloses a method for separating and purifying a nucleoside chemical ingredient from ocean low-value shellfish (such as mactra veneriformis, ruditapes philippinarum, meretrix lusoria, anadara subcrenata, cyclina sinensis, solen and surf clam shell). The method comprises the first step of preparation of shellfish water extract and alcohol precipitate supernate, the second step of macroreticular resin column rough separation and the third step of separation and purification of cation exchange resin. The method for separating and purifying the nucleoside chemical ingredient from the ocean low-value shellfish is reasonable in technological design, strong in operability, short in production period, low in cost and high in environmental protection; the prepared nucleoside chemical ingredient is high in purity and has the good effect of protecting liver; mactra veneriformis, ruditapes philippinarum, meretrix lusoria and other low-value shellfish resources can be sufficiently utilized, and the important economic value is obtained.

Description

technical field [0001] The invention relates to a marine low-value shellfish, in particular to a nucleoside obtained by separating and purifying from marine low-value shellfish (Square clam, Philippine clam, Meretrix meretrix, clam, green clam, razor clam, Xishi tongue, etc.) Chemical composition and its preparation method. Background technique [0002] Jiangsu has a vast coastal area and is rich in marine biological resources in the intertidal zone, especially low-value shellfish resources such as four-cornered clams, Philippine clams, and meretrix meretrix. Marine shellfish and clams have a long history of medicinal use, and there are many records of their efficacy in ancient literature. Modern studies have shown that they have immune regulation, anti-tumor, hypoglycemic, and antioxidant effects. Nucleoside components are not only the basic components of RNA and DNA, but also energy substances in biological oxidation and energy metabolism and intermediates of antiviral an...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N21/31
CPCG01N21/31G01N30/02G01N2030/027
Inventor 刘睿吴皓程建明张倩王欣之杨小林邱韵萦
Owner 浙江海富海洋生物科技有限公司
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