The invention relates to a traditional Chinese medicine compound preparation used for treating colpitis and tinea manus and pedis and a preparation method thereof. The preparation is made from oriental bittersweet, Common Cnidium Fruit, Herba Oldenlandiae, kuh-seng, dandelion, angelica dahurica, phellodendron, giant knotweed, Cortex Dictamni, Radix Stemonae, fructus kochiae, radix cyathulae, ginkgo and borneol. The preparation method adopts the process that part of the impurities are precipitated by water cooking and ethanol solution, alkali liquor is used for adjusting pH value, the borneol is added and finally filtration and sterilization are implemented. With the organic combination of the drugs as well as scientific refinement and being directly acting on a part invaded by pathogenic bacteria, the compound preparation can effectively bring the efficacies of clearing away heat and toxic material, dehumidifying, killing insects and relieving itching into play, has notable treatment effect on trichomonas vaginitis, colpomycosis, gonorrhoea and mycetogenous tinea pedis as well as certain effect on tinea manus and pedis with the treatment rate being up to over 90 percent, and is convenient for administration. The preparation method is simple and convenient in operation, and rational in technological design and retains the effective ingredients of drugs to the maximum extent.