313 results about "Micro electrode" patented technology

The invention relates to the technical field of material detection, in particular to a silver coating adhesive force detection method based on multi-frequency impedance imaging, which comprises the following steps: applying a dual-mode multi-frequency excitation signal to the surface of a silver coating through an attached microelectrode array, synchronously acquiring voltage-current response data, and generating a dual-mode multi-frequency impedance original data set; performing time-varying impedance phase-locked separation on the dual-mode multi-frequency impedance original data set, extracting a dynamic impedance component of a coating-matrix interface region, and reconstructing a three-dimensional sub-interface impedance distribution diagram; and outputting an adhesive force quantized value through an oscillation frequency-adhesive force conversion model according to the spatial oscillation frequency characteristics of the impedance phase in the three-dimensional sub-interface impedance distribution diagram. According to the invention, a double-phase-locked loop system is adopted to realize the time-varying component demodulation of the impedance response of the silver coating-matrix interface, the low-frequency dynamic impedance signal modulated by silver ion migration is effectively extracted, and the weak change of the interface bonding state is sensitively reflected.

The invention relates to the technical field of microfluidics, in particular to a high-throughput and high-efficiency cell extrusion microfluidic system and a particle introduction method. And a closed-loop control system integrating real-time cell deformation sensing, dynamic parameter decision and physical extrusion and alternating electric field cooperative application is constructed. The chip is provided with an inlet shunting device, a micro-channel and a channel structure of a microelectrode. The method comprises the steps of real-time deformation image acquisition and analysis and dynamic extrusion parameter decision and cooperative application. The invention provides a high-flux and high-efficiency cell extrusion microfluidic system and a particle introduction method, and aims to solve the problems that the drug loading efficiency and the survival rate are difficult to consider due to heterogeneity in the existing cell extrusion technology and a physical extrusion and electroporation coordinated regulation strategy is lacked, so that personalized precise treatment of cells is realized, and the cell quality is improved. The drug loading efficiency, the cell survival rate and the high throughput are obviously improved.

The invention discloses a collaborative light regulation and control decision method and system, and relates to the technical field of intelligent regulation and control of plant factory light environments, and the system comprises a plant physiology monitoring unit which is provided with a non-invasive microelectrode array and a hyperspectral imager; and the environment sensing unit is used for collecting illumination intensity, spectrum, temperature, humidity and carbon dioxide concentration data. According to the collaborative light regulation and control decision-making method and system, fusion of multi-source heterogeneous data is realized through a pulse time sequence coding mechanism, and the problem that environmental parameters and plant physiological statuses are difficult to collaboratively analyze in the prior art is solved; based on a dynamic optimization architecture of double-alliance Nash equilibrium, autonomous collaborative decision-making of spectrum parameters can be completed in a short time, and compared with a traditional preset light formula, the response speed is improved; decision effectiveness can be synchronously guaranteed on the virtual model and biological entity level, the light suppression misjudgment rate is reduced, and meanwhile the plant light energy utilization rate is stably improved.

The invention discloses a high-linearity dynamic and static pressure flexible sensor based on Gaussian height distribution. The high-linearity dynamic and static pressure flexible sensor comprises a packaging layer, an upper electrode layer, a piezoelectric layer, a common electrode layer, an insulating layer, a lower electrode layer and a substrate layer which are sequentially stacked from top to bottom, the piezoelectric layer comprises a plurality of PVDF micro-columns, the top ends of the PVDF micro-columns are fixedly connected with the upper electrode layer, the bottom ends of the PVDF micro-columns are provided with micro-electrode layers, and the height of the PVDF micro-columns is of a Gaussian distribution structure from the center to the periphery; the common electrode layer is provided with a plurality of micro-column through holes which are completely overlapped with the PVDF micro-columns; the output leads of the upper electrode layer and the common electrode layer output piezoelectric signals corresponding to dynamic pressure, and the output leads of the common electrode layer and the lower electrode layer output capacitance signals corresponding to static pressure. According to the invention, the dynamic pressure is measured by using the piezoelectricity of PVDF, synchronous measurement of static pressure is realized through a Gaussian distribution structure of a plurality of PVDF micro-columns, and high-sensitivity and high-linearity output of dynamic and static pressure measurement signals is realized.

The utility model relates to the technical field of capacitor processing, in particular to a detection tool for capacitor processing, which comprises a supporting mechanism used for supporting the whole device, the supporting mechanism comprises a workbench and two fixing blocks, and the supporting mechanism is provided with a detection mechanism used for applying pressure to different point positions of a capacitor shell. The detection mechanism comprises two electric telescopic rods. According to the utility model, through the arrangement of the detection mechanism, the device can carry out pressure detection on a plurality of point positions of the capacitor shell, the electric telescopic rod drives the detection head a to carry out pressure detection on the upper part of the capacitor shell, and the bidirectional threaded rod drives the two side plates to move oppositely. And meanwhile, a gear is driven to rotate through a micro electrode, so that a bearing circular plate can drive the capacitor shell to rotate, thereby changing a test point position, reducing manual operation and ensuring the detection effect of the device.

The invention relates to a high-concentration breeding wastewater treatment method, which comprises the following steps of: enabling wastewater to sequentially pass through a coagulation area A, a flocculation area B and a biochemical area C, and respectively carrying out high-speed stirring coagulation, low-speed flocculation and microbiological treatment, so as to construct an integrated wastewater treatment system which is efficient, anti-blocking, compact and high in automation degree. The device comprises a wastewater tank, a multi-stage treatment assembly, a dosing box, a power distribution cabinet and a clear water tank, the multi-stage treatment assembly comprises a supporting frame, a multi-stage treatment box, an aeration assembly and a biological membrane assembly, a stirring assembly can fully stir wastewater, it is ensured that a coagulant and the wastewater are instantly and uniformly mixed, uniform micro-flocs are formed, and bottom sedimentation is avoided; secondly, a unique pulse aggregation environment is created in a microelectrode plate and stirring mode, destabilization and aggregation of colloidal particles can be promoted, and mild conditions are provided for growth and compaction of flocs; and finally, stable and rich microbial communities are formed for the biological membrane assembly, so that the wastewater treatment efficiency is improved.

The invention relates to a graphene hydrogel neural microelectrode and a preparation method thereof.The neural microelectrode comprises a long-strip-shaped electrode body, the electrode body comprises an insulating substrate layer, a conductive layer and an insulating covering layer, and the conductive layer is wrapped and sealed by the insulating substrate layer and the insulating covering layer; a contact window is arranged at one end, close to the electrode body, of the insulating covering layer, a conductive expansion layer made of graphene hydrogel is arranged below the contact window and the insulating covering layer on the periphery of the contact window, the conductive expansion layer is electrically connected with the conductive layer, and the conductive expansion layer can expand after being heated or absorbing water to form an electrode contact protruding outwards from the contact window. Amino multi-walled carbon nanotubes, amino graphene quantum dots and gold nanoparticles are loaded in the graphene hydrogel. The electrode contact has the advantages that after the electrode contact is heated or absorbs water, the conductive expansion layer expands to form the electrode contact protruding out of the contact window, and the electrode contact is good in tissue affinity, capable of making better contact with nervous tissue and more excellent in performance.

A method and system for protecting electrode arrays while implanted on a brain including a flexible electrode array including non-penetrating cortical surface microelectrodes, a ceramic layer covering the electrode array and a polymer layer adjacent the ceramic layer, wherein the ceramic layer and the polymer layer hermetically seal the electrode array. The ceramic layer can be configured to exhibit sufficient flexibility to allow the electrode array to be implanted using minimally invasive surgical techniques, while still hermetically sealing the electrode array from a biological environment.

The invention discloses an ancient tree health state monitoring method based on digital twinning, and relates to the field of ancient tree health monitoring, and the method comprises the steps: collecting point cloud data and texture data of an ancient tree, and constructing an initial three-dimensional model of the ancient tree; a micro ion selective electrode array is deployed in an ancient tree root distribution area, Ca2 and K + ion flow velocity changes are detected through a constant potential rectifier, and the mycorrhizal symbiosis index is calculated in combination with soil temperature and humidity data; uploading the mycorrhizal symbiosis index to a digital twinborn body, and displaying the active state of a root system area in an initial three-dimensional model of the ancient tree in a three-dimensional thermodynamic diagram form; a 16-channel MOX gas sensor electronic nose array is installed on the surface of a trunk of an ancient tree, and volatile organic compound (VOC) concentration data of the ancient tree are collected. According to the invention, a micro electrode array with a 45-degree inclination angle is adopted to non-destructively monitor the ion flow velocity of a root system through the mycorrhizal symbiosis index detection unit, and a three-dimensional thermodynamic diagram is generated in combination with an inverse distance weighted interpolation algorithm, so that dynamic visualization of underground mycorrhizal activity is realized.

The invention discloses a neural microelectrode for electrical stimulation combined with drug release and a preparation method thereof.The neural microelectrode comprises a first flexible substrate layer, a drug-loaded slow-release layer, a second flexible substrate layer, a conductive layer and an insulating covering layer, and the drug-loaded slow-release layer contains chitosan microspheres embedded with drugs; the medicament comprises nerve growth factors and / or anti-inflammatory drugs, a plurality of drug release holes are distributed in the insulating covering layer, an electrode contact is arranged at one end of the conducting layer, a lead connector is electrically arranged at the other end of the conducting layer, and openings for exposing the electrode contact and the lead connector are correspondingly formed in the two ends of the insulating covering layer respectively. According to the neural microelectrode provided by the invention, the drug-loaded slow-release layer is integrated in the neural microelectrode, so that a space for storing a drug does not need to be additionally processed on the membrane layer, and the drug does not need to be additionally loaded, so that the production and processing are relatively convenient and rapid; after the neural microelectrode is implanted into a corresponding tissue in a body, the drug-loaded sustained-release layer can be gradually degraded to slowly release a medicament for repairing nervous tissues or avoiding rejection reaction in a long-acting manner.

The invention relates to the technical field of semiconductor micro-nano manufacturing, and discloses a novel semiconductor test probe manufacturing process which comprises the following steps: preparing an ionic liquid electrolyte containing a high-pressure priority adsorbent and a low-pressure priority adsorbent; a first voltage pulse is applied between the microelectrode and the conductive base, the amplitude of the first voltage pulse enables the high-voltage preferential adsorbent to preferentially adsorb on the surface of the microelectrode, and a flexible probe root and a flexible probe body are formed through deposition; then, a second voltage pulse is applied, and the amplitude of the second voltage pulse is lower than that of the first voltage pulse, so that the low-voltage preferential adsorbent preferentially adsorbs and deposits to form a superhard probe tip. The probe with the functional gradient structure is integrally manufactured by programmed voltage regulation and control in single continuous deposition, high hardness of the needle tip and high flexibility of the needle body are simultaneously realized on a single structure, the manufacturing process is remarkably simplified, and the cost is reduced.

The invention discloses a multifunctional neural electrode for intracranial pressure detection and closed-loop nerve regulation, the multifunctional electrode sequentially comprises a PMUT electrode for intracranial pressure detection and nerve regulation and a macro-micro electrode for electroencephalogram signal detection from top to bottom, and the PMUT electrode is bonded on the macro-micro electrode. The PMUT electrode is bonded to the macro-micro electrode to serve as a multifunctional electrode, the PMUT electrode can serve as a pressure sensor and can also serve as an ultrasonic transducer, the dual functions of intracranial pressure detection and fixed-point ultrasonic nerve stimulation are achieved, the macro-micro electrode can achieve detection of local field potential and peak potential, and the ultrasonic nerve stimulation function is achieved. Therefore, a detection-feedback-regulation closed-loop system is realized.

The invention relates to the technical field of biology, and provides a chip assembly and a preparation method thereof. The chip assembly comprises a substrate, a circuit layer, a culture piece and a microelectrode array. The microelectrode array is located in the culture tank of the culture piece, the culture tank can be used for containing the to-be-monitored organ, and the to-be-monitored organ can make contact with the substrate and make contact with the microelectrode array when located in the culture tank. The substrate and the microelectrode array are both flexible structural members, so that the substrate and the microelectrode array can deform. Therefore, when the organoid is in the culture tank and the organoid is in contact with the microelectrode array, the microelectrode array deforms to be matched with the appearance of the organoid, so that the organoid is in more sufficient contact with the microelectrode array, the signals, collected by the microelectrode array, of the organoid are more accurate, and the damage of the microelectrode array to the organoid can be reduced. The base is also a flexible structural member, and the microelectrode array arranged on the base can move correspondingly by deforming the base so as to adjust the position of the microelectrode array.

The invention discloses an intelligent zinc-aluminum coating quality monitoring method and system for a wind power blade bolt, and solves the problems that internal defects of a coating cannot be found in an early stage and predictive maintenance cannot be realized in the prior art. The method comprises the following steps: acquiring multi-source monitoring data of coating internal defects, interface micro-strain and corrosion states through a terahertz imaging unit, a pre-embedded FBG sensing unit and a microelectrode array ECN acquisition unit which are integrated on an inspection robot; after carrying out local preprocessing by utilizing an edge computing gateway, synchronizing data to a digital twinborn analysis platform deployed with a coating state intelligent evaluation model so as to output a current health state grade and residual life prediction of the coating; finally, a maintenance decision suggestion is automatically generated or an equipment control instruction is triggered based on the result, closed-loop monitoring from state sensing to intelligent execution is formed, and the system comprises a corresponding hardware module and a software platform and is used for achieving the method.

The invention discloses a microelectrode for collecting cell electrophysiological signals and a preparation method thereof.The microelectrode comprises a substrate, an electrode array, a wire array and a bonding pad array, the electrode array, the wire array and the bonding pad array are arranged on the substrate, the electrode array is distributed in a dispersed mode with a preset position as the center, and the wire array is connected with the electrode array and the bonding pad array; the electrode array comprises a first conducting layer, the wire array sequentially comprises a first conducting layer, a second conducting layer and an insulating layer, the bonding pad array sequentially comprises a first conducting layer and a second conducting layer, and the first conducting layer and the second conducting layer are made of different materials. According to the embodiment, the performance of the microelectrode array can be improved, the preparation cost is reduced, and the preparation efficiency is improved. The microelectrode can be widely applied to the technical field of microelectrodes.

The invention discloses a single vesicle particle electrochemical signal identification and classification method and identification system, and the method comprises the steps: S1, preparing a carbon fiber ultramicro electrode, applying a voltage in a patch clamp system constant potential mode, and collecting picoampere-level current response generated by oxidation of a single vesicle content on the surface of the electrode; s2, carrying out smooth filtering on the collected original current signal, and realizing characteristic peak identification and multi-dimensional physical / statistical characteristic extraction by using a multi-parameter peak identification method based on a threshold value; s3, carrying out preprocessing operations including missing value processing, standardization, principal component analysis dimension reduction and category equalization on the extracted peak value characteristic data; and S4, on the basis of the preprocessed feature data, constructing and training at least one machine learning model for automatically identifying the particle size category of the vesicle particles. The method combines the advantages of single-vesicle electrochemical high-sensitivity measurement and intelligent algorithm classification, and has the advantages of high recognition efficiency, good repeatability, wide applicability and the like.

The invention discloses a high-channel clinical microelectrode array intelligent brain function positioning system and method, and belongs to the field of electronic information and the technical field of clinical disease diagnosis and treatment. The system comprises a microelectrode array implanted in the brain, a signal processing and transmission unit and a signal identification unit. The microelectrode array adopts a multi-channel design, so that neural electrophysiological signals and transmitter chemical signals in different depths and directions can be synchronously detected, and the resolution ratio of a single cell level is achieved. The signal processing and transmission unit is responsible for signal acquisition, amplification and transmission and applying electrical stimulation; and the signal identification unit carries out real-time processing on the signal through a convolutional neural network algorithm, rapidly identifies a target nucleus and realizes accurate positioning. The nerve cell discharge and transmitter release can be monitored in real time at different depths and in different directions, rapid and accurate target spot positioning can be achieved by means of an advanced intelligent algorithm, and a more reliable basis is provided for DBS surgery.

The invention discloses a multi-nucleic acid probe rapid modification and multi-target detection device based on electric field regulation and control. An instrument main body in the device is integrated with an automatic liquid path system and a micro electrochemical workstation module, and is used for realizing programmable reagent and sample conveying and multi-channel electrochemical measurement. The detachable sensing chip is formed by bonding a microelectrode array chip and a micro-fluidic chip, and is switched through a PCB (Printed Circuit Board) to realize plug and play. According to the present invention, the positive suction-negative discharge strategy of the electric field is utilized, the preferred positive potential is applied to the target electrode to efficiently attract the probe, and the preferred negative potential is applied to the non-target electrode to inhibit the non-specific adsorption so as to achieve the spatial selective rapid fixation and the accurate density regulation and control of the multi-nucleic acid probe in the extremely short time; the device can be used for quantitatively detecting various body fluid disease markers in a plurality of urine samples at the same time in single operation, and an efficient, accurate and portable multi-marker comprehensive analysis solution is provided for early screening and instant diagnosis of tumors.

The present application relates to the technical field of microelectrode preparation, and particularly relates to a transparent microelectrode array chip preparation method, comprising the following steps: S1. pretreating a glass substrate to improve the substrate surface cleanliness and adsorbability of the electrode film; S2. evaporating an ITO film on the surface of the pretreated glass substrate by using a magnetron sputtering process, as a transparent electrode; S3. spin-coating a photoresist on the surface of the ITO film and performing a patterning treatment, and then etching the ITO film to form a preset electrode structure by using a dry etching process, and then removing the photoresist and cleaning and drying; S4. coating an insulating layer on the surface of the substrate by using a PECVD process, patterning the insulating layer and etching by using a dry etching process to realize windowing, and then removing the photoresist and cleaning and drying; and realizing photoelectric synchronous detection. Avoiding metal residue interference: photoresist masks are used throughout the etching process to replace traditional metal hard masks.

A three-dimensional (3D) microelectrode array device for in vitro electrophysiological applications includes a substrate and micro vias extending from the bottom face to the top face of the substrate. A microneedle at each micro via extends from the bottom face upward beyond the top face and forms a hypodermic microneedle array on the top face. Metallic traces on the bottom face interconnect the hypodermic microneedles to form the 3D microelectrode array. A microheater is positioned on the bottom face of the substrate. Microfluidic ports may be formed at the substrate. Interdigitated electrodes may be formed at the substrate.

The present disclosure is directed to neural interface devices and methods that accesses the subarachnoid space to enable minimally invasive modulation and recording of neural structures. Exemplary embodiments may comprise an implantable pulse generator and a microelectrode catheter. In particular embodiments, the microelectrode catheter comprises one or more stimulating and recording electrodes. Exemplary embodiments may also include methods comprising performing a lumbar puncture to access the spinal subarachnoid space and advancing microcatheter through the spinal subarachnoid space.

The present application relates to the technical field of semiconductor electrostatic chuck, in particular to an electrostatic chuck ceramic plate with microelectrode and a processing method. In the processing, the electrode forming surface of the ceramic layer is first determined, photoresist is coated and dried, and the photoresist reserved area and the exposed area are formed through exposure and development. The exposed area is subjected to metal plating, and then the photoresist in the reserved area is removed. After cleaning, the exposed area is plated with a metal layer to form a microelectrode. The electrostatic, temperature sensing and heating layer plates are independently manufactured into microelectrodes through the process, and then are bonded to form a ceramic plate. The present application replaces the traditional process with the precision process of photoetching and development combined with plating, improves the forming precision and layout density of the microelectrode, realizes independent manufacturing and high-density integration of the microelectrodes of the three types of layer plates, solves the problem of integration of functional electrodes in the prior art, can realize accurate control of multiple temperature zones, has a mature and coherent process, guarantees the forming quality of the microelectrode, improves the chucking and temperature control performance, meets the needs of semiconductor process, and has practicality and mass production value.

The application discloses an electronic tuning type multifunctional sensor and a preparation method thereof. The sensor comprises a quartz crystal, a composite electrode and an oscillation circuit; the first end of the composite electrode is connected with the first end of the oscillation circuit, the second end of the oscillation circuit is connected with the first end of the quartz crystal, the second end of the quartz crystal is connected with the second end of the composite electrode, and the second end of the oscillation circuit is an output end. The composite electrode comprises a substrate, a planar inductive microelectrode and a planar capacitive microelectrode which are arranged on two sides and are connected in series through a via hole; a nano-magnetic iron oxide film is deposited on the inductive side, a hydroxylated ultra-high specific surface area disordered porous carbon film is deposited on the capacitive side, and a perfluorosulfonic acid protective layer is coated on the surfaces of the two films. The application forms a frequency selection network by connecting the double-sided electrode and the quartz crystal in series, and outputs an anti-interference frequency signal; the protective layer is insulated and prevents short circuit on the inductive side, and improves the humidity response on the capacitive side; manual overturning can switch the detection of metal damage, non-metal defects and environmental humidity, and the sensor has the advantages of high stability and low cost.

The invention discloses a method for identifying logging interpretation electrical standard parameters, and solves the problems of inaccurate difference and low efficiency caused by the influence of subjective factors on the manual identification result of the logging interpretation electrical standard parameters in the prior art. The method comprises the following steps: S1, establishing a benchmarking array for a well section to be identified; s2, matching the target well data on the apparent resistivity curve A based on the established benchmarking array; obtaining optimal matching data; s3, determining a reference layer identified by the electrical standard; s4, identifying a microsphere standard, a sound wave calcium judgment standard and a deep three lateral standard; s5, identifying a microelectrode standard; and S6, identifying a micro-gradient calcium judgment standard. The device for realizing the automatic identification of the logging interpretation electrical standard parameters comprises an establishing unit; a matching unit; a determination unit; a first identification unit; a second identification unit; and a third identification unit. According to the method, logging interpretation electrical standard parameters can be rapidly identified, and quality improvement and efficiency improvement of logging interpretation reservoir thickness division work are assisted.

The present invention discloses a microfluidic bio-interconnected neural network chip and a preparation method thereof. The chip consists of a microelectrode array, a microfluidic structure, and a cell culture ring structure. The microelectrode array can detect and stimulate the activity of neurons; the microfluidic structure limits the growth direction of axons between cell culture partitions by setting cell culture partitions, microfluidic channel dimensions, microfluidic channel shapes, and fillet distribution, thereby achieving controllable low-power transmission of neuronal electrical information; the cell culture ring structure provides nutrients for cells in the cell culture chamber. The present invention provides a method for manufacturing a microfluidic bio-interconnected neural network chip, using fully exposed positive photoresist as a sacrificial layer for microfluidic structure peeling, thereby solving the problem of difficult demolding of thin-film microfluidic structures. The chip can be used to explore the response characteristics of neuronal networks to external stimuli, study the internal information transmission mode of neuronal networks, and other fields.

The invention belongs to the technical field of beauty equipment, and particularly relates to a beauty light zirconium ion excitation device which comprises a device shell, the head of the device shell is provided with an ion excitation head, the ion excitation head comprises an insulating base and an excitation electrode group fixed on the insulating base, and the excitation electrode group is formed by arranging a plurality of microelectrodes in an array form; the device shell is internally provided with an ion excitation circuit, the ion excitation circuit comprises a high-voltage generation module and an electrode driving module, the high-voltage generation module is electrically connected with the electrode driving module, and the electrode driving module is respectively connected with each microelectrode in the excitation electrode group through a wire. According to the invention, the electrode group formed by arranging a plurality of independent microelectrodes in a specific array is arranged on the ion excitation head, the electrode driving module is respectively connected with the microelectrodes, and the array formed by the plurality of microelectrodes can physically form an ion excitation source with wider coverage and more dense distribution; and the generated ion current is milder and more stable.

The invention belongs to the technical field of signal detection, and relates to a three-dimensional integrated cell electrophysiology platform and a preparation method and application thereof.The three-dimensional integrated cell electrophysiology platform is composed of microelectrodes and a three-dimensional micro-scaffold, and the microelectrodes comprise a two-dimensional plane microelectrode and a three-dimensional microelectrode; wherein the microelectrode is used for applying stimulation to cells in a two-dimensional plane and a three-dimensional space and collecting electrophysiological signals; the three-dimensional micro-scaffold is used for simulating a three-dimensional microenvironment of normal physiological activities of cells; the average diameter of the two-dimensional planar microelectrode and the average diameter of the three-dimensional microelectrode are each independently 1 [mu] m or less. The three-dimensional integrated cell electrophysiology platform provided by the invention has the functions of high-precision processing, high structural design flexibility and high integration, and is suitable for various scenes such as cell culture, cell detection, cell excitation and the like.

The present invention discloses an in-situ testing system for sediment pore water chemical parameters, comprising a docking fixture, an electric push rod, a guide rail, a connecting rod, a probe, a perforated rubber cover, a large end cap, a microelectrode connector, a small end cap, a copper tube, a water collection funnel, a permeable ceramic, a microelectrode assembly, a fixing sleeve, a flange, an electronics compartment, a connecting block, a submersible pump, and a three-way valve. Based on microelectrode detection technology, the present invention employs an electric push rod to achieve stable vertical displacement of the probe, passively collects pore water through the permeable ceramic, and replaces pore water samples through a pump and valve. The system is used to continuously detect chemical parameters such as pH, H2S, and Eh in marine sediment pore water. The present invention has the advantages of a simple and reliable structure, low cost, strong environmental adaptability, low disturbance, easily replaceable parts, high precision, and high efficiency.

The invention discloses an in-vitro biological neural network accurate regulation and control method and system based on a microelectrode array, and the method comprises the steps: S1, culturing and planting neuronal cells on a microelectrode array chip, and forming an in-vitro neural network with spontaneous activity; s2, preprocessing the in-vitro neural network to enable the in-vitro neural network to enter a stable function state capable of being effectively activated; s3, recording an electrophysiological signal, sampling the structure and function connection of the neural network, and dynamically selecting an optimal stimulation site; s4, applying electrical stimulation to the selected stimulation sites according to a preset regulation and control target, collecting neural network response signals in real time, and constructing a closed-loop regulation and control loop; and S5, performing quantitative analysis on the neural network based on the multi-dimensional evaluation index to form a comprehensive evaluation result, and feeding back the comprehensive evaluation result to the step S4 to optimize a subsequent stimulation strategy. According to the method, organic unification of sensing, intelligent decision making, closed-loop intervention and multi-dimensional quantitative evaluation of a neural network structure and a function state can be realized.

The application provides a wide stress full-linearity flexible ion-dissociation sensor and a preparation method thereof, which comprises a heat-shrinkable film material layer, a flexible AgNWs / MXene electrode, and a dielectric layer, the dielectric layer is attached between the two flexible AgNWs / MXene electrodes, and the two flexible AgNWs / MXene electrodes are provided with corresponding heat-shrinkable film material layers. The electrode of the application adopts the AgNWs / MXene composite electrode with good mechanical properties, the AgNWs microelectrode provides a conductive framework, and the MXene plays a role in protecting the AgNWs from oxidation and corrosion and providing a pseudo-capacitance; the application is based on the ion-dissociation effect of the ion gel film, uses the ion gel film with a porous microstructure as the dielectric layer, so that the dielectric layer has a larger deformable surface area and provides a larger deformation capacity, is thousands of times of the traditional solid-state similar sensor, and makes the ion-dissociation sensor have better linearity, higher sensitivity and faster response speed.