390 results about "Micro electrode" patented technology

The invention relates to the technical field of corrosion monitoring, in particular to an intelligent pipeline corrosion monitoring and protection system which comprises a dynamic potential disturbance measurement module, a local microcell scanning module, a corrosion rate calculation module, a corrosion risk distribution analysis module and an environmental parameter compensation module. According to the invention, accurate measurement is realized, the spatial resolution is improved, and the current response change rate is calculated by measuring the reference potential of the surface of the pipeline, applying instantaneous disturbance, combining current change differential operation, dynamically tracking the corrosion process, improving the real-time response capability and arranging a microelectrode array to cover corrosion hot spots; the corrosion current density is solved by combining the electrochemical characteristics of the material, the corrosion rate is dynamically predicted, regional distribution is analyzed based on the change of the corrosion rate, the corrosion risk assessment precision is optimized, environmental factors are monitored, disturbance parameters are adjusted, interference is compensated, the monitoring accuracy under different working conditions is ensured, and data stability and protection measure optimization are guaranteed.

The invention provides a cognitive function in-vitro simulation method based on a microelectrode array (MEA) and brain organs and application of the cognitive function in-vitro simulation method, and belongs to the crossing field of brain organ in-vitro model construction, neural engineering, brain-like intelligence and the like. In an in-vitro environment, external signal (such as sound) information is converted into electrical stimulation input capable of being perceived by brain organs in an MEA electrical stimulation mode, so that the brain organs gradually learn and identify external signals in repeated electrical stimulation; after elution stimulation for a period of time, the reduction condition of recognition accuracy is observed, namely, the important memory and forgetting process in the cognitive function is simulated. According to the method, the electrophysiological response and the biological mechanism change of the brain organs under external electrical stimulation can be quantitatively evaluated, and a new possibility is provided for exploring the advanced cognitive function by utilizing the brain organs.

The invention relates to the technical field of material detection, in particular to a silver coating adhesive force detection method based on multi-frequency impedance imaging, which comprises the following steps: applying a dual-mode multi-frequency excitation signal to the surface of a silver coating through an attached microelectrode array, synchronously acquiring voltage-current response data, and generating a dual-mode multi-frequency impedance original data set; performing time-varying impedance phase-locked separation on the dual-mode multi-frequency impedance original data set, extracting a dynamic impedance component of a coating-matrix interface region, and reconstructing a three-dimensional sub-interface impedance distribution diagram; and outputting an adhesive force quantized value through an oscillation frequency-adhesive force conversion model according to the spatial oscillation frequency characteristics of the impedance phase in the three-dimensional sub-interface impedance distribution diagram. According to the invention, a double-phase-locked loop system is adopted to realize the time-varying component demodulation of the impedance response of the silver coating-matrix interface, the low-frequency dynamic impedance signal modulated by silver ion migration is effectively extracted, and the weak change of the interface bonding state is sensitively reflected.

The invention provides a system and method for detecting free carbon particles in transformer oil based on a microelectrode array, and the system comprises a sampling module which comprises an oil inlet pipeline and a filtering device, an oil sample is conveyed to a microelectrode array sensor module from a transformer oil tank through the oil inlet pipeline, and the filtering device is located in the oil inlet pipeline; a filter membrane is arranged in the filter device to remove impurities in the oil sample; the microelectrode array sensor module comprises a microelectrode array sensor, the microelectrode array sensor is nanoelectrodes arranged on a silicon substrate, the surface of the microelectrode array sensor is coated with graphene oxide to adsorb free carbon particles in an oil sample, and when the free carbon particles are adsorbed to the surface of the microelectrode array sensor, an adsorption signal is generated; and the signal processing unit is used for extracting the characteristic frequency and intensity of the vibration of the carbon particles in the adsorption signal, and classifying the carbon particles according to the characteristic frequency and intensity of the carbon particles. The device is compact in structure, the maintenance cost is reduced, and the operation process is simplified.

The invention discloses a multi-brain organ electron complex as well as a construction method and application thereof. The multi-brain organ electronic complex comprises a microelectrode array and a fusion organ attached to the surface of a chip electrode of the microelectrode array through a PDMS film, and the fusion organ can grow for more than or equal to 120 days through culture. The PDMS film is attached to the surface of the MEA chip electrode, so that the stability of combination of the MEA chip electrode and the organoid is remarkably enhanced, the construction efficiency of a multi-brain organoid electron complex is improved, and the continuity of neural signal detection is improved; a high-performance model platform is provided for multi-brain organ research, and the method has a wide application prospect.

The invention relates to the technical field of microfluidics, in particular to a high-throughput and high-efficiency cell extrusion microfluidic system and a particle introduction method. And a closed-loop control system integrating real-time cell deformation sensing, dynamic parameter decision and physical extrusion and alternating electric field cooperative application is constructed. The chip is provided with an inlet shunting device, a micro-channel and a channel structure of a microelectrode. The method comprises the steps of real-time deformation image acquisition and analysis and dynamic extrusion parameter decision and cooperative application. The invention provides a high-flux and high-efficiency cell extrusion microfluidic system and a particle introduction method, and aims to solve the problems that the drug loading efficiency and the survival rate are difficult to consider due to heterogeneity in the existing cell extrusion technology and a physical extrusion and electroporation coordinated regulation strategy is lacked, so that personalized precise treatment of cells is realized, and the cell quality is improved. The drug loading efficiency, the cell survival rate and the high throughput are obviously improved.

The invention discloses a collaborative light regulation and control decision method and system, and relates to the technical field of intelligent regulation and control of plant factory light environments, and the system comprises a plant physiology monitoring unit which is provided with a non-invasive microelectrode array and a hyperspectral imager; and the environment sensing unit is used for collecting illumination intensity, spectrum, temperature, humidity and carbon dioxide concentration data. According to the collaborative light regulation and control decision-making method and system, fusion of multi-source heterogeneous data is realized through a pulse time sequence coding mechanism, and the problem that environmental parameters and plant physiological statuses are difficult to collaboratively analyze in the prior art is solved; based on a dynamic optimization architecture of double-alliance Nash equilibrium, autonomous collaborative decision-making of spectrum parameters can be completed in a short time, and compared with a traditional preset light formula, the response speed is improved; decision effectiveness can be synchronously guaranteed on the virtual model and biological entity level, the light suppression misjudgment rate is reduced, and meanwhile the plant light energy utilization rate is stably improved.

The invention discloses a microelectrode structure and a manufacturing method thereof and a sensor, the microelectrode structure comprises a flexible substrate and a microelectrode system arranged on the surface of the flexible substrate, the microelectrode system comprises a counter electrode, a working electrode and a reference electrode, and electrode units are horizontally laid on the upper surface and the lower surface of the flexible substrate. The electrode units on the upper surface and the lower surface of the flexible substrate respectively comprise at least one of a counter electrode, a working electrode and a reference electrode, the electrode units on the upper surface of the flexible substrate form a first microelectrode system, and the electrode units on the lower surface of the flexible substrate form a second microelectrode system; or the electrode units on the upper surface and the lower surface of the flexible substrate jointly form a third microelectrode system. The electrodes are manufactured on the front side and the back side of the flexible substrate to form one or two microelectrode systems, the area of the microelectrodes is maximized on the limited surface of the substrate, and the detection precision is improved; double-channel double-substance monitoring, double-channel single-substance monitoring or single-channel single-substance monitoring can be achieved, and functional diversity is achieved.

The invention discloses a high-linearity dynamic and static pressure flexible sensor based on Gaussian height distribution. The high-linearity dynamic and static pressure flexible sensor comprises a packaging layer, an upper electrode layer, a piezoelectric layer, a common electrode layer, an insulating layer, a lower electrode layer and a substrate layer which are sequentially stacked from top to bottom, the piezoelectric layer comprises a plurality of PVDF micro-columns, the top ends of the PVDF micro-columns are fixedly connected with the upper electrode layer, the bottom ends of the PVDF micro-columns are provided with micro-electrode layers, and the height of the PVDF micro-columns is of a Gaussian distribution structure from the center to the periphery; the common electrode layer is provided with a plurality of micro-column through holes which are completely overlapped with the PVDF micro-columns; the output leads of the upper electrode layer and the common electrode layer output piezoelectric signals corresponding to dynamic pressure, and the output leads of the common electrode layer and the lower electrode layer output capacitance signals corresponding to static pressure. According to the invention, the dynamic pressure is measured by using the piezoelectricity of PVDF, synchronous measurement of static pressure is realized through a Gaussian distribution structure of a plurality of PVDF micro-columns, and high-sensitivity and high-linearity output of dynamic and static pressure measurement signals is realized.

The invention discloses a micro-matrix electrode for biological tissue electric signal mapping, and belongs to the technical field of bioelectrophysiological detection.The micro-matrix electrode comprises a substrate, a matrix micro-needle, a plurality of bonding pads and a plurality of wires, the matrix micro-needle is arranged on the end face of the operation end of the substrate, and the bonding pads are symmetrically arranged on the upper surface and the lower surface of the substrate respectively; the matrix microneedle is composed of a plurality of microneedles which are arranged according to a 4 * 4 square matrix, and each microneedle is connected with the corresponding bonding pad through a wire. The embodiment of the invention also discloses a preparation method of the micro-matrix electrode. The preparation method comprises the following steps: S1, processing the conversion connection part and the operation part; s2, preparing a matrix microneedle; s3, preparing a bonding pad; s4, aerosol is sprayed and deposited to obtain a plurality of wires, one ends of every two wires are connected with the corresponding microneedles, the other ends of every two wires are connected with the corresponding bonding pads, and then the micro-matrix electrode is obtained.

The utility model relates to the technical field of capacitor processing, in particular to a detection tool for capacitor processing, which comprises a supporting mechanism used for supporting the whole device, the supporting mechanism comprises a workbench and two fixing blocks, and the supporting mechanism is provided with a detection mechanism used for applying pressure to different point positions of a capacitor shell. The detection mechanism comprises two electric telescopic rods. According to the utility model, through the arrangement of the detection mechanism, the device can carry out pressure detection on a plurality of point positions of the capacitor shell, the electric telescopic rod drives the detection head a to carry out pressure detection on the upper part of the capacitor shell, and the bidirectional threaded rod drives the two side plates to move oppositely. And meanwhile, a gear is driven to rotate through a micro electrode, so that a bearing circular plate can drive the capacitor shell to rotate, thereby changing a test point position, reducing manual operation and ensuring the detection effect of the device.

The invention discloses a cognitive state monitoring and intervention device and method. The cognitive state monitoring and intervention device comprises a microelectrode drug delivery array module, a wireless transceiving and control circuit module and a power supply module, a microcontroller is embedded in the wireless transceiving and control circuit module; the microelectrode drug delivery array module comprises a hollow drug delivery microneedle, a sensing electrode and a controlled drug delivery electrode; the hollow drug delivery microneedle comprises a hollow microneedle array and a drug storage chamber; the sensing electrode is coupled and fixed on a substrate of the hollow microneedle array, and is attached to the body surface to monitor skin impedance signals when in use, liquid medicine is stored in the medicine storage chamber, and a controlled administration electrode is mounted in the medicine storage chamber; when the wireless transceiving and control circuit module applies current to the controlled dosing electrode, the controlled dosing electrode and the liquid medicine generate electrochemical reaction, and the liquid medicine is pumped into the body surface through the microneedle. According to the invention, the cognitive function state of the body can be continuously monitored, and accurate administration can be automatically realized.

The invention relates to a high-concentration breeding wastewater treatment method, which comprises the following steps of: enabling wastewater to sequentially pass through a coagulation area A, a flocculation area B and a biochemical area C, and respectively carrying out high-speed stirring coagulation, low-speed flocculation and microbiological treatment, so as to construct an integrated wastewater treatment system which is efficient, anti-blocking, compact and high in automation degree. The device comprises a wastewater tank, a multi-stage treatment assembly, a dosing box, a power distribution cabinet and a clear water tank, the multi-stage treatment assembly comprises a supporting frame, a multi-stage treatment box, an aeration assembly and a biological membrane assembly, a stirring assembly can fully stir wastewater, it is ensured that a coagulant and the wastewater are instantly and uniformly mixed, uniform micro-flocs are formed, and bottom sedimentation is avoided; secondly, a unique pulse aggregation environment is created in a microelectrode plate and stirring mode, destabilization and aggregation of colloidal particles can be promoted, and mild conditions are provided for growth and compaction of flocs; and finally, stable and rich microbial communities are formed for the biological membrane assembly, so that the wastewater treatment efficiency is improved.

The invention discloses a hydrogel modified microelectrode array device and a preparation method thereof, the hydrogel modified microelectrode array device comprises a microelectrode array composite layer, a passivation layer, a hydrogel layer and a cell culture ring, the cell culture ring is arranged on the microelectrode array composite layer, and the passivation layer and the hydrogel layer are located in the cell culture ring; the microelectrode array composite layer comprises a microelectrode array with polydimethylsiloxane as a base material, at least part of the microelectrode array in the cell culture ring is exposed, and at least part of gaps of the exposed microelectrode array are filled with the passivation layer; the hydrogel layer grows on the surface of the polydimethylsilane, and a microgroove is formed in the exposed microelectrode array and passivation layer area to serve as a cell growth area. According to the invention, cell patterning can be realized on the microelectrode array, cell electrophysiological activity can be monitored for a long time, and the microelectrode array has the advantages of remarkable cell patterning promotion capability, high stability and robustness, flexible cell pattern design, excellent biological compatibility and simple process.

The invention relates to a graphene hydrogel neural microelectrode and a preparation method thereof.The neural microelectrode comprises a long-strip-shaped electrode body, the electrode body comprises an insulating substrate layer, a conductive layer and an insulating covering layer, and the conductive layer is wrapped and sealed by the insulating substrate layer and the insulating covering layer; a contact window is arranged at one end, close to the electrode body, of the insulating covering layer, a conductive expansion layer made of graphene hydrogel is arranged below the contact window and the insulating covering layer on the periphery of the contact window, the conductive expansion layer is electrically connected with the conductive layer, and the conductive expansion layer can expand after being heated or absorbing water to form an electrode contact protruding outwards from the contact window. Amino multi-walled carbon nanotubes, amino graphene quantum dots and gold nanoparticles are loaded in the graphene hydrogel. The electrode contact has the advantages that after the electrode contact is heated or absorbs water, the conductive expansion layer expands to form the electrode contact protruding out of the contact window, and the electrode contact is good in tissue affinity, capable of making better contact with nervous tissue and more excellent in performance.

The invention provides a salt spray environment parameter detection micro-nano sensor and a preparation and measurement method thereof, the salt spray environment parameter detection micro-nano sensor comprises a silicon substrate, the upper surface and the lower surface of the silicon substrate are respectively provided with a SiO2 insulation layer and a first Si3N4 film; the first Pt resistor is arranged on the upper surface of the SiO2 insulating layer, a second Si3N4 thin film is deposited on the upper surface of the first Pt resistor, and first Au interdigital electrodes are arranged on the two sides, in the length direction of the silicon substrate, of the first Pt resistor; the second Pt resistor is arranged on the lower surface of the first Si3N4 film; the third Pt resistor is arranged on the upper surface of the adhesion layer on the SiO2 insulating layer, a third Si3N4 thin film is deposited on the surface of the third Pt resistor, a patterned second Au interdigital electrode is arranged on the upper surface of the third Si3N4 thin film, and a PI thin film is deposited on the upper surface of the second Au interdigital electrode. The device has the beneficial effects that the wetting time is shortened through the'hollow-window-flower-like 'interdigital microelectrodes, high-precision measurement of the temperature and heat exchange of the sensor body and salt mist gas can be realized, the influence of temperature and humidity on conductivity detection is eliminated through data compensation, the desorption process is accelerated, and wet stagnation is reduced.

A method and system for protecting electrode arrays while implanted on a brain including a flexible electrode array including non-penetrating cortical surface microelectrodes, a ceramic layer covering the electrode array and a polymer layer adjacent the ceramic layer, wherein the ceramic layer and the polymer layer hermetically seal the electrode array. The ceramic layer can be configured to exhibit sufficient flexibility to allow the electrode array to be implanted using minimally invasive surgical techniques, while still hermetically sealing the electrode array from a biological environment.

The invention discloses an ancient tree health state monitoring method based on digital twinning, and relates to the field of ancient tree health monitoring, and the method comprises the steps: collecting point cloud data and texture data of an ancient tree, and constructing an initial three-dimensional model of the ancient tree; a micro ion selective electrode array is deployed in an ancient tree root distribution area, Ca2 and K + ion flow velocity changes are detected through a constant potential rectifier, and the mycorrhizal symbiosis index is calculated in combination with soil temperature and humidity data; uploading the mycorrhizal symbiosis index to a digital twinborn body, and displaying the active state of a root system area in an initial three-dimensional model of the ancient tree in a three-dimensional thermodynamic diagram form; a 16-channel MOX gas sensor electronic nose array is installed on the surface of a trunk of an ancient tree, and volatile organic compound (VOC) concentration data of the ancient tree are collected. According to the invention, a micro electrode array with a 45-degree inclination angle is adopted to non-destructively monitor the ion flow velocity of a root system through the mycorrhizal symbiosis index detection unit, and a three-dimensional thermodynamic diagram is generated in combination with an inverse distance weighted interpolation algorithm, so that dynamic visualization of underground mycorrhizal activity is realized.

The invention discloses a neural microelectrode for electrical stimulation combined with drug release and a preparation method thereof.The neural microelectrode comprises a first flexible substrate layer, a drug-loaded slow-release layer, a second flexible substrate layer, a conductive layer and an insulating covering layer, and the drug-loaded slow-release layer contains chitosan microspheres embedded with drugs; the medicament comprises nerve growth factors and / or anti-inflammatory drugs, a plurality of drug release holes are distributed in the insulating covering layer, an electrode contact is arranged at one end of the conducting layer, a lead connector is electrically arranged at the other end of the conducting layer, and openings for exposing the electrode contact and the lead connector are correspondingly formed in the two ends of the insulating covering layer respectively. According to the neural microelectrode provided by the invention, the drug-loaded slow-release layer is integrated in the neural microelectrode, so that a space for storing a drug does not need to be additionally processed on the membrane layer, and the drug does not need to be additionally loaded, so that the production and processing are relatively convenient and rapid; after the neural microelectrode is implanted into a corresponding tissue in a body, the drug-loaded sustained-release layer can be gradually degraded to slowly release a medicament for repairing nervous tissues or avoiding rejection reaction in a long-acting manner.

The invention relates to the technical field of semiconductor micro-nano manufacturing, and discloses a novel semiconductor test probe manufacturing process which comprises the following steps: preparing an ionic liquid electrolyte containing a high-pressure priority adsorbent and a low-pressure priority adsorbent; a first voltage pulse is applied between the microelectrode and the conductive base, the amplitude of the first voltage pulse enables the high-voltage preferential adsorbent to preferentially adsorb on the surface of the microelectrode, and a flexible probe root and a flexible probe body are formed through deposition; then, a second voltage pulse is applied, and the amplitude of the second voltage pulse is lower than that of the first voltage pulse, so that the low-voltage preferential adsorbent preferentially adsorbs and deposits to form a superhard probe tip. The probe with the functional gradient structure is integrally manufactured by programmed voltage regulation and control in single continuous deposition, high hardness of the needle tip and high flexibility of the needle body are simultaneously realized on a single structure, the manufacturing process is remarkably simplified, and the cost is reduced.

The invention discloses a multifunctional neural electrode for intracranial pressure detection and closed-loop nerve regulation, the multifunctional electrode sequentially comprises a PMUT electrode for intracranial pressure detection and nerve regulation and a macro-micro electrode for electroencephalogram signal detection from top to bottom, and the PMUT electrode is bonded on the macro-micro electrode. The PMUT electrode is bonded to the macro-micro electrode to serve as a multifunctional electrode, the PMUT electrode can serve as a pressure sensor and can also serve as an ultrasonic transducer, the dual functions of intracranial pressure detection and fixed-point ultrasonic nerve stimulation are achieved, the macro-micro electrode can achieve detection of local field potential and peak potential, and the ultrasonic nerve stimulation function is achieved. Therefore, a detection-feedback-regulation closed-loop system is realized.

The invention relates to the technical field of biology, and provides a chip assembly and a preparation method thereof. The chip assembly comprises a substrate, a circuit layer, a culture piece and a microelectrode array. The microelectrode array is located in the culture tank of the culture piece, the culture tank can be used for containing the to-be-monitored organ, and the to-be-monitored organ can make contact with the substrate and make contact with the microelectrode array when located in the culture tank. The substrate and the microelectrode array are both flexible structural members, so that the substrate and the microelectrode array can deform. Therefore, when the organoid is in the culture tank and the organoid is in contact with the microelectrode array, the microelectrode array deforms to be matched with the appearance of the organoid, so that the organoid is in more sufficient contact with the microelectrode array, the signals, collected by the microelectrode array, of the organoid are more accurate, and the damage of the microelectrode array to the organoid can be reduced. The base is also a flexible structural member, and the microelectrode array arranged on the base can move correspondingly by deforming the base so as to adjust the position of the microelectrode array.

The invention discloses an intelligent zinc-aluminum coating quality monitoring method and system for a wind power blade bolt, and solves the problems that internal defects of a coating cannot be found in an early stage and predictive maintenance cannot be realized in the prior art. The method comprises the following steps: acquiring multi-source monitoring data of coating internal defects, interface micro-strain and corrosion states through a terahertz imaging unit, a pre-embedded FBG sensing unit and a microelectrode array ECN acquisition unit which are integrated on an inspection robot; after carrying out local preprocessing by utilizing an edge computing gateway, synchronizing data to a digital twinborn analysis platform deployed with a coating state intelligent evaluation model so as to output a current health state grade and residual life prediction of the coating; finally, a maintenance decision suggestion is automatically generated or an equipment control instruction is triggered based on the result, closed-loop monitoring from state sensing to intelligent execution is formed, and the system comprises a corresponding hardware module and a software platform and is used for achieving the method.

The invention discloses a microelectrode for collecting cell electrophysiological signals and a preparation method thereof.The microelectrode comprises a substrate, an electrode array, a wire array and a bonding pad array, the electrode array, the wire array and the bonding pad array are arranged on the substrate, the electrode array is distributed in a dispersed mode with a preset position as the center, and the wire array is connected with the electrode array and the bonding pad array; the electrode array comprises a first conducting layer, the wire array sequentially comprises a first conducting layer, a second conducting layer and an insulating layer, the bonding pad array sequentially comprises a first conducting layer and a second conducting layer, and the first conducting layer and the second conducting layer are made of different materials. According to the embodiment, the performance of the microelectrode array can be improved, the preparation cost is reduced, and the preparation efficiency is improved. The microelectrode can be widely applied to the technical field of microelectrodes.

The invention discloses a neural microelectrode based on a silicon nanostructure and a preparation method of the neural microelectrode. The neural microelectrode comprises a silicon substrate with the silicon nanostructure, and a first insulating layer, a conductive layer and a second insulating layer which are sequentially stacked on the silicon substrate, wherein the silicon nanostructure is a silicon nanocolumn structure, and the second insulating layer partially covers the conductive layer, so that a stimulation site, a recording site and a welding site of the conductive layer are exposed. According to the invention, the silicon substrate with the silicon nanostructure is used as a substrate material of the neural microelectrode, the silicon nanostructure is a high-density three-dimensional nanostructure formed by the silicon nanorod array, the diameter and height of the silicon nanorod can be flexibly adjusted, and the silicon nanorod has a relatively large specific surface area, so that the overall effective surface area of the neural microelectrode is increased; furthermore, the impedance of the microelectrode is reduced and the electrochemical performance of the microelectrode is improved.

The invention discloses a single vesicle particle electrochemical signal identification and classification method and identification system, and the method comprises the steps: S1, preparing a carbon fiber ultramicro electrode, applying a voltage in a patch clamp system constant potential mode, and collecting picoampere-level current response generated by oxidation of a single vesicle content on the surface of the electrode; s2, carrying out smooth filtering on the collected original current signal, and realizing characteristic peak identification and multi-dimensional physical / statistical characteristic extraction by using a multi-parameter peak identification method based on a threshold value; s3, carrying out preprocessing operations including missing value processing, standardization, principal component analysis dimension reduction and category equalization on the extracted peak value characteristic data; and S4, on the basis of the preprocessed feature data, constructing and training at least one machine learning model for automatically identifying the particle size category of the vesicle particles. The method combines the advantages of single-vesicle electrochemical high-sensitivity measurement and intelligent algorithm classification, and has the advantages of high recognition efficiency, good repeatability, wide applicability and the like.

The invention discloses a high-channel clinical microelectrode array intelligent brain function positioning system and method, and belongs to the field of electronic information and the technical field of clinical disease diagnosis and treatment. The system comprises a microelectrode array implanted in the brain, a signal processing and transmission unit and a signal identification unit. The microelectrode array adopts a multi-channel design, so that neural electrophysiological signals and transmitter chemical signals in different depths and directions can be synchronously detected, and the resolution ratio of a single cell level is achieved. The signal processing and transmission unit is responsible for signal acquisition, amplification and transmission and applying electrical stimulation; and the signal identification unit carries out real-time processing on the signal through a convolutional neural network algorithm, rapidly identifies a target nucleus and realizes accurate positioning. The nerve cell discharge and transmitter release can be monitored in real time at different depths and in different directions, rapid and accurate target spot positioning can be achieved by means of an advanced intelligent algorithm, and a more reliable basis is provided for DBS surgery.

The invention discloses a multi-nucleic acid probe rapid modification and multi-target detection device based on electric field regulation and control. An instrument main body in the device is integrated with an automatic liquid path system and a micro electrochemical workstation module, and is used for realizing programmable reagent and sample conveying and multi-channel electrochemical measurement. The detachable sensing chip is formed by bonding a microelectrode array chip and a micro-fluidic chip, and is switched through a PCB (Printed Circuit Board) to realize plug and play. According to the present invention, the positive suction-negative discharge strategy of the electric field is utilized, the preferred positive potential is applied to the target electrode to efficiently attract the probe, and the preferred negative potential is applied to the non-target electrode to inhibit the non-specific adsorption so as to achieve the spatial selective rapid fixation and the accurate density regulation and control of the multi-nucleic acid probe in the extremely short time; the device can be used for quantitatively detecting various body fluid disease markers in a plurality of urine samples at the same time in single operation, and an efficient, accurate and portable multi-marker comprehensive analysis solution is provided for early screening and instant diagnosis of tumors.

The invention discloses a transparent culture device supporting cell orientation and electrophysiological signal in-situ monitoring and a preparation method. The transparent culture device comprises a transparent substrate, a microelectrode structure is prepared on the transparent substrate, oriented biological fibers are prepared on the microelectrode structure, and a cell culture cavity used for containing cell culture fluid is formed above the oriented biological fibers and the microelectrode structure; the directional biological fiber and microelectrode structure arrangement mode is designed according to requirements and is printed and formed, and a transparent substrate material is selected according to requirements; according to the invention, cell orientation and electrophysiological signal in-situ monitoring are supported on the transparent culture surface through electrostatic printing, and normal cell observation is not affected.

The present application relates to the technical field of microelectrode preparation, and particularly relates to a transparent microelectrode array chip preparation method, comprising the following steps: S1. pretreating a glass substrate to improve the substrate surface cleanliness and adsorbability of the electrode film; S2. evaporating an ITO film on the surface of the pretreated glass substrate by using a magnetron sputtering process, as a transparent electrode; S3. spin-coating a photoresist on the surface of the ITO film and performing a patterning treatment, and then etching the ITO film to form a preset electrode structure by using a dry etching process, and then removing the photoresist and cleaning and drying; S4. coating an insulating layer on the surface of the substrate by using a PECVD process, patterning the insulating layer and etching by using a dry etching process to realize windowing, and then removing the photoresist and cleaning and drying; and realizing photoelectric synchronous detection. Avoiding metal residue interference: photoresist masks are used throughout the etching process to replace traditional metal hard masks.