Cell potential measuring electrode and measuring apparatus using the same
a cell potential and measuring electrode technology, applied in the field of low impedance cell potential measuring electrode assembly, can solve the problems of difficult to measure plural positions simultaneously, difficult long term measurement of cell potential, damage to cells, etc., and achieve the effect of reducing manufacturing costs, reducing labor intensity and reducing labor intensity
Inactive Publication Date: 2003-02-04
PANASONIC CORP
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Benefits of technology
Preferably the microelectrodes and the reference electrodes are formed from the same material to both simplify the manufacturing process and obtain a cost benefit. Preferably, the microelectrodes and the reference electrodes are formed of layers of nickel plating, gold plating, and platinum black on an indium-tin oxide (ITO) film. After platinization, the impedance of the reference electrodes is preferably between 2 and 3 kilohms.
The insulating substrate (...
Problems solved by technology
Measurement of cell potential by inserting a glass electrode or the like into the cell may damage that cell.
Long term measurement of cell potential is quite difficult.
It is further difficult to measure plural positions simultaneously; there is a limit to the number of electrodes one can place in a measurement electrode array and it is similarly difficult to adequately determine the position of the sample over measurement electrodes.
However, when measuring very low level or micro-potentials such as cell potentials, noise is a problem.
As mentioned...
Method used
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This Example shows the difference in noise level between a system including an integrated multiple electrode such as that discussed above and reference electrodes of 50 microns square and 200 microns square. FIG. 9 and FIG. 10 show those comparative noise levels.
We fabricated integrated multiple electrodes (such as shown in FIG. 4) having, respectively, reference electrodes of 50 microns square and reference electrodes of 200 microns square. The integrated multiple electrodes each had cylindrical members 6. The same culture medium as normally used in tissue culturing was placed inside the cylindrical members 6. To limit the resulting signal to the noise, no cell or tissue sample was placed on the microelectrodes. As shown in FIG. 11, of the 64 microelectrodes, the central seven sites (channels I to 5, 7, 8) were measured.
FIG. 9 shows the noise waveform of the reference electrodes in 50 microns square, and FIG. 10, 200 microns square. In each diagram, the voltage on the axis of ordin...
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Abstract
This invention relates to a low impedance cell potential measuring electrode assembly typically having a number of microelectrodes on an insulating substrate and having a wall enclosing the region including the microelectrodes. The device is capable of measuring electrophysiological activities of a monitored sample using the microelectrodes while cultivating those cells or tissues in the in the region of the microelectrodes. The invention utilizes independent reference electrodes to lower the impedance of the overall system and to therefore lower the noise often inherent in the measured data. Optimally the microelectrodes are enclosed by a physical wall used for controlling the atmosphere around the monitored sample.
Description
FIELD OF THE INVENTIONThis invention relates to a low impedance cell potential measuring electrode assembly typically having a number of microelectrodes on an insulating substrate and having a wall enclosing the region including the microelectrodes. The device is capable of measuring electrophysiological activities of a monitored sample using the microelectrodes while cultivating those cells or tissues in the in the region of the microelectrodes. The invention utilizes independent reference electrodes to lower the impedance of the overall system and to therefore lower the noise often inherent in the measured data. Optimally the microelectrodes are enclosed by a physical wall used for controlling the atmosphere around the monitored sample.BACKGROUND OF THE INVENTIONCell potential measuring apparatus have been developed to measure the activity or electrical potential generated by activity of nerve cells, other cells, or tissues (for example, Japanese Kokai 8-62209) without inserting g...
Claims
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Login to view more IPC IPC(8): C12M1/34G01N27/403
CPCG01N33/4836C12M41/46
Inventor SUGIHARA, HIROKAZUOKA, HIROAKISHIMONO, KENOGAWA, RYUTATAKETANI, MAKOTO
Owner PANASONIC CORP
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