564 results about "Biosafety" patented technology

The invention provides a bio-based adhesive and a preparation method thereof. Inspired by a spider thread structure, biomass adhesive interface enhancement based on a tannic acid adhesion group is combined with construction of a differential phase separation structure by biomass-induced polylipoic acid depolymerization, and a brand new strategy of adhesion-cohesion synergistic enhancement of the adhesive strength of the adhesive is realized. According to the invention, tannic acid and carboxymethyl chitosan are introduced into a lipoic acid-based adhesive, so that the purpose of synergistically enhancing the bonding performance of the adhesive through adhesion-cohesion is achieved, and the defects of weak cohesion, low bonding strength, poor water resistance, poor moisture resistance and the like of the conventional biomass adhesive are overcome. Benefited from a new strategy of adhesion-cohesion synergistic enhancement, the biomass adhesive has good adhesive strength in various environments and materials. In addition, the biomass adhesive also has the characteristics of biological safety, antibacterial and mildew-proof properties and recoverability, so that the product has wider application and potential compared with the previous product, and has a good application prospect.

The invention provides a recombinant XVII type collagen and application thereof, and relates to the technical field of protein engineering. The novel recombinant human XVII type collagen is obtained by optimizing and screening collagen structural domain sequences of the human XVII type collagen and then combining the sequences. The recombinant human XVII type collagen is consistent with the corresponding part of the amino acid sequence of the human XVII type collagen, does not generate immunological rejection when being applied to a human body, and can be widely applied to the industries of surgical medical treatment and medical beauty. In addition, the recombinant human XVII type collagen is high in stability, and the problem of protein degradation in the process of expressing recombinant protein by pichia pastoris can be avoided. In addition, the recombinant human XVII type collagen also has the effects of high biological activity, high biological safety, promotion of cell proliferation and differentiation and promotion of hair growth, and can be applied to the field of cosmetics or medicines as a functional component.

The invention discloses a biosafety laboratory airflow risk monitoring method and device, a medium and a product, and relates to the field of data processing. The method comprises the following steps: acquiring airflow sensor data and facility state data, calculating a restraint integrity index according to the two types of data, performing dynamic time warping matching on a real-time change curve of the index and a preset baseline database, outputting a matching degree, and when the matching degree is lower than a preset threshold value, predicting that an airflow restraint barrier has a fracture risk, positioning a risk source according to the data source causing the abnormal matching degree, taking the source as a release point of a virtual aerosol pollutant, executing fluid dynamic simulation in combination with real-time environment data, generating a three-dimensional space-time diffusion trajectory of the pollutant in a laboratory, and extracting a risk quantitative index; and accurate laboratory airflow risk early warning information is generated based on the quantitative indexes. According to the invention, early warning can be carried out before the risk occurs, and the problem of safety hysteresis in airflow risk monitoring of the biosafety laboratory is relieved.

The invention relates to the field of medical instruments, in particular to a heart valve. The invention provides a heart valve. The heart valve comprises a stent body, a skirt edge and developing points. The skirt edge is connected to the bracket body; the skirt edge comprises an inner skirt edge and an outer skirt edge; the inner skirt edge and the outer skirt edge form an interlayer; the developing points are arranged in the interlayer; the number of the developing points is at least two; at least two developing points are in the same or different annular cross sections. Developing points are arranged in an interlayer; the setting mode can physically isolate the developing point, and the direct contact between the bracket and the developing point is eliminated through physical isolation; the galvanic corrosion risk can be effectively avoided, and the service life of instruments is prolonged; the metal ion release risk is reduced, and the biological safety is improved. And the developing points are arranged in the interlayer between the inner skirt edge and the outer skirt edge, so that the overall position of the valve can be reflected more accurately.

The invention discloses an antibiotic slow-release system based on hierarchical porous silicon dioxide and a preparation method of the antibiotic slow-release system, and belongs to the technical field of biological medicines. The antibiotic slow-release system comprises a spherical hierarchical-pore silicon dioxide microsphere carrier, amino functional groups covalently bonded to the outer surface and the inner surface of a pore channel of the spherical hierarchical-pore silicon dioxide microsphere carrier, antibiotic molecules loaded in the pore channel of the spherical hierarchical-pore silicon dioxide microsphere carrier, and a pH-responsive chitosan gating layer. The preparation method comprises the following steps: (1) preparing a hierarchical pore silicon dioxide microsphere carrier; (2) aminating the surface of the carrier; (3) loading antibiotics; and (4) constructing the pH responsive chitosan gating layer. According to the invention, the silicon dioxide carrier with a macroporous-mesoporous hierarchical structure is constructed to realize high drug loading capacity, and the pH-responsive chitosan gating layer is covalently bonded at the orifice of the silicon dioxide carrier to realize intelligent controlled release, so that the problem that high drug loading capacity, low burst release rate and biological safety are difficult to achieve at the same time in the prior art is solved.

The invention belongs to the field of medicines, and discloses application of pyroglutamic acid in preparation of products for inhibiting fat deposition. The invention has the following advantages: 1, obesity induced by high fat diet is prevented; 2, pyroglutamic acid is a natural amino acid derivative, widely exists in animal and plant bodies, and has good biological safety; meanwhile, the invention further discloses a product for inhibiting fat deposition.

The invention relates to the technical field of nano materials and biological medicines, in particular to black phosphorus-loaded monatomic nano enzyme as well as a preparation method and application thereof. According to the monatomic nano-enzyme loaded with the black phosphorus, the monatomic nano-enzyme loaded with the black phosphorus takes a black phosphorus nanosheet as a carrier, and a metal simple substance is anchored on the surface of the carrier; wherein the metal elementary substance comprises any one of iron, cobalt and manganese. A metal active center is anchored on a black phosphorus nano-carrier, the catalytic activity of monatomic nano-enzyme and the photo-thermal conversion performance of a black phosphorus nano-material are effectively combined, the anti-tumor effect is jointly enhanced through chemical kinetic catalysis and photo-thermal, in addition, a loaded black phosphorus degradation product is mainly phosphate and has biological safety, and the application prospect is broad. The toxicity problem of the traditional nano-enzyme can be solved; the wide application is facilitated.

The invention relates to the technical field of biology, and particularly provides a targeting carrier, a targeting drug, a preparation method and application. The targeting carrier comprises (a) a metal-polyphenol compound particle and (b) a targeting structure, and the targeting structure is connected with the outer surface of the metal-polyphenol compound particle. Under the condition that the effectiveness of the LNP based on the cationic lipid and / or the ionizable lipid is not lower than that of the LNP based on the cationic lipid and / or the ionizable lipid, the targeting carrier provided by the invention does not use the cationic lipid and the ionizable lipid, so that the toxicity is greatly reduced, the biological safety is remarkably improved, negative charge drugs are more favorably carried in a living body, the application range is wide, and the targeting carrier can be used for drugs with different sizes. The targeted drug can achieve high expression quantity of nucleic acid drugs, the biological safety is remarkably improved, the targeting property is good, and efficient treatment of various diseases can be achieved.

The invention discloses a composite medical suture and a preparation method thereof, and belongs to the field of biomedicine. In order to solve the problems that an existing medical suture is poor in antibacterial effect and biological safety, the composite medical suture comprises a core layer, a degradation regulation and control layer, a slow release layer and an antibacterial layer which are sequentially arranged from inside to outside; the core layer is prepared from a blend fiber of poly (hydroxybutyrate-hydroxyvalerate) and polycaprolactone-co-polyglycolide; the degradation regulation and control layer comprises a mixture of at least two kinds of polyethylene glycol with different molecular weights; the slow release layer comprises quaternary ammonium salt loaded graphene oxide nanofibers; the antibacterial layer comprises titanium dioxide loaded with silver nanoparticles. Through the synergistic effect of the four layers, the comprehensive optimization of mechanical properties, degradation rate, drug sustained release and antibacterial function is realized; the purposes of improving the slow-release antibacterial performance, reducing damage to the human body, reducing inflammation and the like are achieved, and the antibacterial and degradation effects meet the recovery requirements.

The invention provides a mineralized collagen cartilage repair scaffold and a preparation method and application thereof, and relates to the technical field of biomedical materials.The preparation method comprises the steps that a collagen solution subjected to mineralization treatment is injected into a mold, and a mineralized collagen layer is obtained through deep freezing and primary freeze drying; taking the mineralized collagen layer as a bottom layer, injecting a collagen solution which is not mineralized, and performing deep freezing and first freeze drying to obtain a freeze-dried scaffold; and carrying out crosslinking and secondary freeze drying on the freeze-dried scaffold, and compressing to obtain the mineralized collagen cartilage repair scaffold. A necessary bioactive interface, a continuous interpenetrating structure of a collagenous fiber network and cross-linking treatment are provided for osseointegration, and interlayer separation is avoided; bidirectional regeneration of osteochondral bone is realized, and complexity and risk of clinical application are reduced; in a wet state, good tailorability and enough stiffness are achieved, and meanwhile stable mechanical support is provided; the technical problem that in the prior art, a stent material is insufficient in systematicness of biosecurity of multi-scale structure bionics and interface bonding agents is solved.

The invention relates to glass powder as well as a preparation method and application thereof. The glass powder is prepared from raw materials including an inorganic mixture and glass clinker, by taking the total mass of the preparation raw materials of the glass powder as 100wt%, the percentage contents of the inorganic mixture and the oxides contained in the glass clinker are as follows: 40wt%-54wt% of SiO2, 5wt%-9wt% of Al2O3, 10wt%-30wt% of SrO, 5wt%-8wt% of B2O3, 5wt%-15wt% of ZrO2, 0wt%-5wt% of CaO, 1wt%-8wt% of MgO, 1wt%-2wt% of Y2O3 and 0wt%-5wt% of a sodium-potassium mixture. The glass powder provided by the invention does not contain barium element, and has good biological safety, X-ray developing capability and low-temperature chemical stability. According to the preparation method provided by the invention, impurities are prevented from entering the glass powder, and the glass powder is high in quality and good in batch stability.

The utility model relates to the technical field of animal harmless treatment, and discloses horizontal high-grade biosafety laboratory animal residue treatment equipment, which comprises a cabin body, a feeding port, a discharging port, a feeding port and a discharging port, the support frame is arranged at the lower part of the cabin body; the separation sealing door is arranged in the cabin body, and the feeding port and the discharging port are located on the left side and the right side of the separation sealing door; the crushing device is arranged above the discharge hole; and the feeding device is used for moving materials from the feeding hole to the crushing device on the other side of the separation sealing door. The separation sealing door is arranged in the cabin body, the feeding device and the smashing device are arranged on the left side and the right side of the separation sealing door, the feeding device can enable animal carcasses to be subjected to high-temperature and high-pressure sterilization treatment and then convey the animal carcasses to the smashing device to be smashed, and safety in the animal carcass treatment process is guaranteed; and the operation complexity is reduced.

The invention relates to the technical field of public health safety, and discloses a biological safety event emergency response virtual simulation training system, which comprises a virtual environment construction module, a practical operation training management module, an intelligent evaluation feedback module, a case teaching engine module, a self-adaptive optimization module and an evaluation report generation module, a light source self-calibration module is arranged, optical response models made of different materials are configured for different protective devices, and the authenticity reference of visual presentation of various protective devices is guaranteed; meanwhile, by comparing the wavelength of the virtual light source with a preset spectrum reference value in real time and dynamically monitoring the illumination color temperature deviation of an operation area, the accuracy of aerosol leakage risk assessment is ensured, the misjudgment risk of emergency training is further reduced, and by calculating the spatial position deviation value of a virtual viewpoint of a user and a pollution core area, the risk assessment accuracy is improved. Whether the observation visual angle deviates from the biosafety observation standard axis or not is judged in real time, so that the system can effectively avoid the situation of key pollution path recognition omission.

The invention relates to the technical field of thrombus mining robots, in particular to a micron robot with an asymmetric cilia structure, a preparation method and application. The micro-robot is of an asymmetric composite structure and is composed of a smooth hemisphere and a cilia-shaped structure, sandwich type layered arrangement of a PLGE cilia-shaped nano structure, an MNPs interlayer and a PS sphere core is formed on the surface and the interior of the micro-robot from outside to inside, and cooperative regulation and control of morphology and functions are achieved by regulating and controlling the material proportion of PS and PLGE. The thrombus mining robot shows an efficient thrombolysis effect in a plurality of in-vitro and in-vivo models, and rapid fibrinolysis, deep thrombus penetration and efficient mining of single red blood cells are realized, so that the limitation of a traditional thrombolysis agent is overcome. In addition, the thrombus digging robot has excellent biocompatibility and biological safety, has no obvious cytotoxicity or damage to main organs, and supports the safety of the thrombus digging robot in clinical application.

The invention discloses a method for simultaneously producing ergothioneine and an enzyme preparation by utilizing trichoderma reesei, and belongs to the technical field of biological engineering. By optimizing the culture medium, a culture medium formula capable of enabling the trichoderma reesei which has almost no ergothioneine production capacity originally to synthesize the ergothioneine is screened out, and the trichoderma reesei can simultaneously produce the ergothioneine and an enzyme preparation. By further increasing the number of ergothioneine gene clusters of the trichoderma reesei, under the condition of not introducing any exogenous gene, an industrial production optimized strain of the ergothioneine and the enzyme preparation conforming to food safety is constructed, the yield of the ergothioneine and the enzyme preparation can be remarkably improved, and the production cost of the ergothioneine and the enzyme preparation is reduced. Meanwhile, the problems of biological safety, transgenosis safety and the like are solved, and technical support is provided for industrial production of ergothioneine and enzyme preparations.

The utility model discloses a biological safety dissecting bag which comprises a bag body. An inflation support frame is wrapped in the bag body, the inflation support frame comprises a plurality of communicated inflation columns, and one inflation column is provided with an inflation valve; a zipper is arranged on the end face and / or the side face of the bag body close to the bottom, sealing strips are arranged on the bag body on the two sides of the zipper, a sealing adhesive tape is arranged on the bottom face of one sealing strip, and the sealing strip is wide enough to cross the zipper to be attached to the top face of the sealing strip on the other side through the sealing adhesive tape. At least one pair of gloves is arranged on each of the front, rear, left and right surfaces of the bag body, the gloves are provided with glove openings, glove openings are formed in the bag body at the joints of the gloves and the bag body, and the glove openings are in sealed connection with the glove openings. The device is suitable for being used in dissecting scenes with biological safety requirements, common dissecting scenes, sites where dissecting is inconvenient and the like for rapidly creating dissecting conditions and other situations where dissecting platforms need to be set up, and is suitable for being used in hospitals, sites where patients are inconvenient to transfer and other situations where operations are carried out.

The invention discloses a synthetic method of photosensitive composite hydrogel and application of the photosensitive composite hydrogel in treatment of periodontitis. The composition expression of the photosensitive composite hydrogel is MHA-B-coated Lipo, and the photosensitive composite hydrogel is prepared by the following steps: entrapping berberine hydrochloride by lipidosome with proton gradient, and then mixing with methacrylated hyaluronic acid, a photoinitiator and a cross-linking agent; according to the liposome with the proton gradient, a citric acid buffer solution is adopted for preparing blank liposome, and then alkali liquor is used for adjusting the pH value of an external phase to be alkalescent, so that the transmembrane gradient is formed. The liposome is used as a drug delivery carrier, berberine is entrapped in the liposome and is combined with the MHA hydrogel to form a composite sustained-release drug, and the composite sustained-release drug has the advantages of strong targeting property, good responsiveness, good biocompatibility and high biological safety; and the hydrogel has injectable and photosensitive cross-linking characteristics, is suitable for clinical minimally invasive operation, has the advantages of accurate immune regulation and control, and has a wide application prospect.

The invention discloses a multi-index fusion-based biotechnology product risk dynamic identification method and system, and relates to the technical field of biological safety management, and the method comprises the steps: S1, obtaining experimental data and environmental data from each stage of a transgenic crop life cycle, constructing a dynamic data set containing a molecular structure sequence, a function performance index and an ecological impact factor, and carrying out the construction of a dynamic data set; extracting the mutation frequency of the molecular structure sequence along with the time change, the abnormal fluctuation of the function expression index and the dynamic change trend of the ecological influence factor; s2, according to molecular structure sequence mutation frequency and functional performance index abnormal fluctuation, extracting a nucleotide replacement mode and a functional abnormality data set of the gene editing product in a research and development stage and an application stage, and determining a nucleotide replacement mode and a functional abnormality association mode; according to the biotechnology product risk dynamic identification method and system based on multi-index fusion, accurate prediction and dynamic management of ecological risks of transgenic crops are realized, and the environmental release safety is remarkably improved.

The invention discloses EGCG-myricetin carrier-free nanoparticles based on hydrogen peroxide oxidation crosslinking as well as a preparation method and application of the EGCG-myricetin carrier-free nanoparticles. The preparation method comprises the following steps: mixing amino acid, EGCG and hydrogen peroxide for reaction to prepare a covalent skeleton of an EGCG-amino acid network; and subjecting myricetin and the covalent skeleton of the EGCG-amino acid network to self-assembly so as to obtain the EGCG-myricetin carrier-free nanoparticles based on hydrogen peroxide oxidation crosslinking. According to the EGCG-myricetin carrier-free nano-particles disclosed by the invention, complex molecular design and tedious preparation schemes of a current delivery system and potential harmful effects of excipients are avoided, the prepared carrier-free nano-particles have good loading effect, stability and biological safety, and meanwhile, the EGCG-myricetin carrier-free nano-particles can obviously improve mouse colitis symptoms induced by DSS; technical support is provided for developing carrier-free nano-particles and relieving inflammatory bowel diseases, and the carrier-free nano-particles have huge clinical development prospects.

The invention provides a method for high-throughput rapid screening of food fermentation strains. The method comprises the following steps: mixing a to-be-detected bacterial sample with a culture medium without a yeast product, injecting an obtained mixed bacterial liquid and an oil phase into a liquid drop generation chip, generating liquid drops, and collecting the liquid drops for culture; injecting the cultured liquid drops, the oil phase and SYTOTM16 fluorescent dye liquid into a liquid drop micro-injection chip, collecting the generated liquid drops, injecting the liquid drops and the oil phase into a micro-fluidic sorting chip, carrying out fluorescence detection, collecting target liquid drops, and carrying out demulsification treatment and coating culture. The method can realize rapid and accurate high-throughput screening of target strains, and especially can directly quantify the growth activity of microorganisms under the stress of acid and salt, thereby realizing efficient and accurate screening of acid-resistant and salt-resistant strains. Moreover, the method does not need to carry out gene modification on the strain, avoids the problems of biological safety and technical complexity possibly caused by gene manipulation, retains the natural characteristics of the strain, is suitable for large-scale high-throughput bacterium screening, and has high application value.

The invention discloses a clinical laboratory specimen full-process tracking and biosafety early warning management system, and relates to the technical field of medical biosafety management, and the system comprises a multi-modal data collection module which integrates various devices and comprehensively collects specimens and related data; the intelligent identification and verification module adopts an advanced algorithm to process the image, completes compliance judgment and traceability verification, and outputs a comprehensive data packet; the dynamic risk assessment module calculates a risk coefficient and matches a five-color early warning level; the grading early warning and linkage disposal module determines an early warning grade, quantifies disposal intensity, and realizes visual management and control and closed loop; according to the method, accurate determination of specimen compliance and full-process traceability verification are realized through deep analysis of an algorithm, data quality and management accuracy are improved, meanwhile, a dynamic risk assessment mechanism is constructed, risks are graded accurately, disposal intensity is quantified, risk visual management and control and closed-loop management are realized, and normalization and controllability of biological safety management are enhanced.

The utility model discloses a high-fidelity quick sampling device of a high-grade biological safety dissecting room, which relates to the technical field of biological sampling devices, and comprises a barrel, a first end of the barrel is provided with a cavity, an inlet communicated with the cavity is arranged at the first end of the barrel, scale marks are arranged outside the barrel, a first cutting part is arranged at the first end of the barrel, and a second cutting part is arranged at the second end of the barrel. The first cutting part has a cutting state and an idle state; the operation part is connected with the cylinder body, the operation part and the inlet are arranged in an avoiding manner, and a barrier layer is arranged between the operation part and the cylinder body; the blocking layer enables substances such as pathogens not to be attached to the operation part, the operation part is detachably connected with the second end of the cylinder body and the blocking layer, and therefore the situation that the pathogens attached to the cylinder body and the blocking layer are attached to the next biological tissue, errors are caused to follow-up identification and analysis of the next biological tissue can be avoided as far as possible. And high fidelity during sampling of different biological tissues is realized.

The invention relates to the technical field of biology, in particular to an MDCK cell strain and application of the MDCK cell strain in preparation of influenza vaccines. The invention provides an MDCK cell strain, the preservation number of which is CCTCC NO: C202504. The cell strain not only can maintain high sensitivity and high yield to influenza viruses and ensure the yield and quality of vaccines, but also can remarkably improve the biological safety of cell matrix influenza vaccines due to elimination of tumorigenicity risk, and has a good application prospect. And a key technical support is provided for promoting domestic research and development and marketing of influenza vaccines based on cell culture.

The invention discloses a DNA molecular marker rapid detection system and method based on imaging spectrum light splitting, and belongs to the technical field of molecular biological detection. The system comprises an exciting light array 1, a receiving and transmitting integrated detection light path 2, an optical fiber transmission array 3, a light splitting module 4, spectral data processing software 5 and a push-broom structure 6. The excitation light array 1 realizes excitation of different labeled samples; the receiving and transmitting integrated detection light path 2 reflects and focuses the array exciting light to a sample and converges fluorescence generated by the sample to an optical fiber input end; the optical fiber transmission array 3 realizes single-row multi-sample fluorescence transmission; the light splitting module 4 is used for dispersing exciting light and fluorescent light of multiple samples in a single row; the spectral analysis processing software 5 is used for acquiring and preprocessing data; the push-sweeping mechanism 6 realizes row-by-row detection of multiple rows of samples. According to the technology, high-throughput, high-sensitivity and rapid detection of the DNA molecular marker is realized. The method is suitable for the fields of fake material evidence identification, food and biological safety and the like, and has the advantages of simplicity and convenience in operation, strong anti-interference performance and the like.

The present invention belongs to the field of biomedicine and provides radioactive silica microspheres coated with biomedical polymer materials, as well as a preparation method and application thereof. The method comprises the following steps: S1, mixing silica microspheres, water, and a radioactive nuclide to obtain a radioactive silica microsphere mixture; S2, mixing the radioactive silica microsphere mixture with an alkaline solution and reacting to obtain radioactive silica microspheres; S3, mixing the radioactive silica microspheres with a biomedical polymer solution and coating the mixture to obtain radioactive silica microspheres coated with biomedical polymer materials. The preparation method provided by the present invention is simple, and the prepared radioactive silica microspheres coated with biomedical polymer materials have a high radionuclide labeling rate, a low escape rate, good radiostability, and excellent biosafety, and can be used for the embolization treatment of all solid tumors.

Disclosed herein is a waste encapsulation method comprising: (i) placing an adhesive composition into a compactor; (ii) placing one or more flexible containers containing waste material into the compactor; (iii) heating the binder composition to liquefy the binder composition and embedding the waste into the liquefied binder composition to form a coated mixture; and (iv) compacting the coating mixture and forming a hydrophobic composite article containing the waste material.

This invention discloses an antimicrobial peptide PA targeting multidrug-resistant Gram-negative bacteria and its application. The amino acid sequence of the antimicrobial peptide PA is shown in SEQ ID NO.1. The antimicrobial peptide PA provided by this invention has a significant antibacterial effect against multidrug-resistant Gram-negative bacteria, and has a rapid bactericidal onset, no hemolysis or cytotoxicity, does not cause nephrotoxicity after treatment, and has good biosafety. It can be used for the treatment of bacterial infections and can also be applied to other scenarios that require bactericidal or antibacterial growth inhibition.

This invention provides a negative pressure isolation live microscope experimental box. The experimental box, through its closed negative pressure design, ensures the biosafety of experiments with pathogens of BSL-2 level and above, meeting the requirements of the safety committee review. At the same time, its innovative box-type integrated window structure eliminates the vibration torque caused by animal movement in traditional suspended windows from a mechanical design perspective, providing unparalleled stability for high-precision imaging. Ultimately, it makes it possible to safely and stably conduct live microscopic studies of highly pathogenic infection models in a conventional laboratory.

The present application relates to the technical field of intelligent identification system of crop pests, in particular to a plant field pest fine-grained identification method, system, device and storage medium based on deep learning. The identification method provided by the present application is specialized in high-precision identification of real field scenes, and can provide technical support for important work such as future development of field inspection robot, automatic identification and monitoring system of field pests and the like. In addition to pest monitoring, the field biological safety test of genetically modified plants is gradually carried out at present, and by using the identification method provided by the present application, the dynamic change of farmland insect community can be quickly and accurately identified and predicted, so that the efficiency and accuracy of ecological investigation are greatly improved.

The invention is applicable to the field of genetic engineering, and provides a mycobacterium tuberculosis Mce1A-LS protein nanoparticle, a preparation method and application thereof, the mycobacterium tuberculosis Mce1A-LS protein nanoparticle is formed by sequence fusion of mycobacterium tuberculosis Mce1A protein and LS protein, and the amino acid sequence of the mycobacterium tuberculosis Mce1A-LS protein nanoparticle is shown as SEQ ID NO: 2 in a sequence table. The Mce1A-LS protein nanoparticle provided by the invention is high in immunogenicity, the protein Mce1A with high immunogenicity can induce to generate a synergistic immune effect and is high in safety, and the expressed fusion protein is non-toxic and harmless, so that the biosafety is high, in addition, the immune effect of the Mce1A-LS protein nanoparticle is good, and after the Mce1A-LS protein nanoparticle is used for immunizing a mouse, the immunogenicity of the Mce1A-LS protein nanoparticle is greatly improved. Strong body fluid and / or cellular immune response can be generated, which indicates that after immunization, a specific immune effect can be generated in a mouse body.