SARS-CoV-2 neutralizing antibody detection kit

Abstract

The invention relates to an SARS-CoV-2 neutralizing antibody detection kit. The SARS-CoV-2 neutralizing antibody detection kit comprises a solid phase carrier, an S protein antigen of SARS-CoV-2 and acompetitive substance. The competitive substance is marked with a signal substance and can be specifically combined with the new coronavirus S protein antigen. Whether a tested person is infected bythe new coronavirus or not and whether infection risks exist or not are judged by detecting a neutralizing antibody through an immunodiagnosis technology, and the method is reliable in theory, practical and feasible and can be completed only in a secondary biosafety laboratory.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular, to a SARS-CoV-2 neutralizing antibody detection kit. Background technique [0002] The coronavirus family is divided into 4 genera, alpha, beta, gamma, and delta. The coronaviruses of the alpha and beta genera can infect mammals and humans. Severe acute respiratory syndrome coro-navirus 2 (SARS-CoV-2) is a new type of coronavirus belonging to the genus β. Similar to SARS-CoV that appeared in 2002, SARS-CoV-2 belongs to the subgenus Sarbecovirus (Coronaviridae, genus Coronaviridae), and both rely on the ACE2 protein on the surface of human cells as a cell entry receptor. Protein sequence analysis found that the amino acid similarity of 7 conserved non-structural proteins between SARS-CoV-2 and SARS-CoV was 94.6%. [0003] The existing detection methods for SARS-CoV-2 mainly include nucleic acid detection and antibody detection. The main methods are fluorescent PCR, enzyme-linke...

AI Technical Summary

Problems solved by technology

[0007] Currently established coronavirus neutralizing antibody detection methods include enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA), plaque reduction neutralization test (PRNT), pseudovirus neutralizing antibody detection (PBNA), etc. Among them, both ELISA and IFA detect specific binding antibodies, which cannot be directly applied to the detection of neutralizing antibodies against the new coronavirus; although PRNT detects neutralizing antibodies, since this method is based on live viruses, it needs to be tested The BSL-3 laboratory is completed, and the PRNT detection cycle is too long, and the error of plaque counting is too large, which brings difficulties to many researchers

Although the counterfeit virus neutralization test uses a safer fake virus to replace it, although it does not need to be completed in a BSL-3 laboratory, the detection cycle is also longer and takes 2-4 days, which cannot meet the needs of public screening.

The detection of neutralizing antibodies to the new coronavirus is generally based on the above methods, and there is no simpler and more reliable method to establish