23 results about "Immunodiagnostics" patented technology

The invention relates to the technical fields of gene engineering, antibody engineering and immunodiagnosis. The invention provides a recombinant humanized anti-Cpn IgM monoclonal antibody. The monoclonal antibody comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are respectively shown as SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5; the heavy chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are shown as SEQ ID NO.6, SEQ ID NO.7 and SEQ ID NO.8 respectively. The invention also provides a method for preparing the monoclonal antibody. The recombinant humanized anti-Cpn IgM monoclonal antibody provided by the invention is expressed in mammalian cells by utilizing a gene recombination technology, has the characteristics of high purity, small batch difference and the like, and can be applied to a Cpn IgM antibody detection kit as a quality control product; as a quality control product, the recombinant humanized anti-Cpn IgM monoclonal antibody has the advantages of high sensitivity, high safety, inter-batch stability and the like.

The present disclosure relates to a diagnostic cartridge for immunodiagnosis, and a reader and a diagnostic system using the same. A diagnostic cartridge, including the diagnostic pad and the color reaction pad, is provided to detect a color reaction and a fluorescence reaction through the single diagnostic cartridge.

The invention discloses an antibody pair, a reagent and a method for detecting helicobacter pylori, and relates to the field of immunodiagnosis. The antibody pair for detecting the helicobacter pylori comprises the first antibody and the second antibody, and the reagent and the detection method based on the antibody pair can accurately detect the existence of the helicobacter pylori.

This invention relates to a high-titer prealbumin antibody purification method and its application, belonging to the field of biotechnology. The method utilizes Protein A affinity chromatography to capture total IgG from antiserum, followed by purification using an affinity chromatography column prepared by targeted conjugation of a specific sequence peptide. The antibody purified by this method achieves a titer of 1:256,000 in ELISA detection, with a half-maximal effective concentration (EC50). 50 The antibody concentration was 3.2 ng / mL, exhibiting extremely high affinity and reactivity. When used in a double-antibody sandwich chemiluminescent immunoassay, it achieved a detection limit of 0.08 µg / mL, a wide dynamic range (0.08–400 µg / mL), and a titer retention rate as high as 88% after accelerated storage at 37°C for 14 days, demonstrating significantly better stability than antibodies prepared by traditional methods. This invention provides a highly active and stable core antibody raw material for the preparation of high-performance immunodiagnostic reagents.

The invention belongs to the technical field of medical immunodiagnostic reagents, and discloses a freeze-dried VB12 rapid detection kit and a VB12 detection method. According to the kit provided by the invention, in the preparation process of a freeze-dried microsphere detection reagent, trehalose, glucose, gelatin, triton, polyethylene glycol, Proclin 950, 5-bromo-5-nitro-1, 3-dioxane, mannitol and glycine in a specific mass ratio are adopted as a freeze-drying solution system; stress damage to VB12 binding protein and a VB12 compound antibody in the freeze-drying process is reduced, the stability of the reagent in the preparation and storage process is improved, meanwhile, quick redissolution of the freeze-drying reagent can be achieved, and a detection system formed by compounding a reagent storage solution, a freeze-drying microsphere redissolution solution and a sample dissociation solution has the excellent anti-interference capacity and can be used for detecting the VB12 binding protein and the VB12 compound antibody in the freeze-drying process. The obtained detection kit has the advantages of excellent detection sensitivity, reliability, stability and the like, and has a very excellent practical application prospect.

The invention discloses differential temperature comparison single molecule detection based on a reversible thermal change renaturation query probe, and relates to the field of single molecule immunodiagnosis. According to the invention, the property of repeated thermal denaturation / renaturation of the query probe is utilized, and the specific binding activity of the query probe is controlled, so that a specific binding molecular signal and a non-specific binding molecular signal of the query probe are distinguished, and a novel high-specificity single-molecule immunodiagnosis method is provided.

The utility model relates to the technical field of immunoassay instruments, in particular to a detection and analysis device with an anti-pollution function for immunodiagnosis, which comprises a fluorescence immunoassay instrument, a kit, a sealing assembly and a cleaning plate, the fluorescence immunoassay analyzer is provided with a detection area, the detection area is rotatably provided with a cover plate, the bottom of the detection area is provided with a placing groove, the placing groove is used for placing a kit, and the detection area is provided with a sliding cavity; a detection sample is linearly placed in the kit; a sealing assembly is arranged above the detection area, the sealing assembly is located above the kit, and when the cover plate closes the detection area, the sealing assembly seals the kit, the kit is sealed through the cover plate and the sealing assembly, so that the airtightness of the kit is guaranteed in the detection process of the fluorescence immunoassay analyzer, and the detection accuracy is improved. The reagent is prevented from volatilizing into the detection area and depositing in the detection area, so that the influence on subsequent light detection analysis of the reagent is avoided.

This invention relates to the field of immunodiagnostics and provides recombinant HIV antigens for HIV antibody detection. Specifically, it concerns recombinant HIV antigens, nucleic acid molecules, expression vectors, host cells, reagents, kits, and their use. When recombinant HIV antigen proteins are used as the labeling end, the marker activity of the recombinant HIV antigen can be significantly improved, and nonspecific immunobinding activity can be reduced, thereby increasing sensitivity.

The application discloses an antibody for detecting RhD blood group antigen and application thereof, and relates to the technical field of immunodiagnosis. The antibody for detecting RhD blood group antigen provided by the application comprises a heavy chain complementarity determining region and a light chain complementarity determining region, and the antibody provides an important raw material source for the detection of RhD blood group antigen and has improved low-temperature stability.

The invention provides a recombinant antigen, a kit containing the same and application, and belongs to the technical field of immunodiagnosis. The invention particularly relates to a recombinant antigen containing rraB or MysB as a fusion partner, a recombinant nucleic acid molecule, a recombinant expression vector, a recombinant host cell, a marker conjugate, a kit and application. When the recombinant antigen is used for immunodiagnosis, the activity and specificity of immunodiagnosis can be remarkably improved.

The invention belongs to the technical field of gene engineering, antibody engineering and immunodiagnosis, and discloses a recombinant humanized monoclonal antibody for resisting treponema pallidum membrane protein TP17 and application of the recombinant humanized monoclonal antibody for resisting the treponema pallidum membrane protein TP17. The sequence of a light chain amino acid fragment of the monoclonal antibody is shown as SEQ ID NO.11, and the sequence of a light chain amino acid fragment of the monoclonal antibody is shown as SEQ ID NO.12. The sequence of a light chain amino acid fragment of the monoclonal antibody is shown as SEQ ID NO.13. The sequence of a heavy chain amino acid fragment of the monoclonal antibody is as shown in SEQ ID NO. 12. The invention further discloses the monoclonal antibody, nucleic acid encoded by the monoclonal antibody, a recombinant expression vector carrying the nucleic acid and a host cell of the recombinant expression vector. The monoclonal antibody has high specificity and high detection sensitivity, and can be applied to preparation of a treponema pallidum detection kit and detection of treponema pallidum.

This invention discloses an antibody that binds to a T4-T4 antibody complex and its applications, relating to the field of immunodiagnostics. The antibody that binds to the T4-T4 antibody complex disclosed in this invention includes a heavy chain variable region and a light chain variable region. This antibody exhibits good activity, detection sensitivity, and clinical relevance, providing an important source of raw materials for the detection of T4.

This application relates to the field of tumor immunodiagnostic technology, and particularly to a monoclonal antibody, polynucleotide, preparation method, and application of antiphosphatidylinositol proteoglycan-3; the monoclonal antibody includes a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region includes heavy chain region CDR1 with amino acid sequence as shown in SEQ ID NO:1, heavy chain region CDR2 with amino acid sequence as shown in SEQ ID NO:2, and heavy chain region CDR3 with amino acid sequence as shown in SEQ ID NO:3; the light chain variable region includes light chain region CDR1 with amino acid sequence as shown in SEQ ID NO:4, light chain region CDR2 with amino acid sequence as shown in SEQ ID NO:5, and light chain region CDR3 with amino acid sequence as shown in SEQ ID NO:6; the dissociation constant KD(M) of the monoclonal antibody against GPC3 antigen is 1.21E-11, which is a high-affinity N-terminal antibody, suitable for the detection of GPC3 in clinical serum samples.

Provided herein are compositions comprising protein encapsulated nanoparticles, and methods of making said compositions. In an aspect, provided herein are compositions comprising cytokine encapsulated nanoparticles, and methods of making said compositions. Further provided herein are methods of monitoring an immunophenotype of a subject and associated immunodiagnostic assays and immunodiagnostic compositions.

The present invention relates to an immune reaction analysis device. The analysis device of the present invention comprises: an incubation chamber which has a plurality of slits for accommodating cartridges, and in which an incubation for an immunodiagnostic reaction is performed; a cartridge input driving unit for inputting the cartridge into the slit of the incubation chamber; a determination unit for determining the result of the reaction in the cartridge; a cartridge withdrawal driving unit for withdrawing the cartridge accommodated in the slit of the incubation chamber; a cartridge transfer driving unit for transferring the withdrawn cartridge to the determination unit; and a control unit for controlling the cartridge input driving unit, the cartridge withdrawal driving unit, and the cartridge transfer driving unit. The immune reaction analysis device of the present invention provides excellent ease of use and can accurately perform a good number of incubations.

The invention relates to the technical field of immunodiagnosis, and discloses a preparation method and application of a colloidal carbon labeled antibody for detecting a bladder tumor antigen. The preparation method of the colloidal carbon labeled antibody comprises the following steps: adding polyvinylpyrrolidone into a carbon powder aqueous dispersion, adjusting the pH value to 6.0-6.5, then adding an anti-bladder tumor antigen antibody, and labeling; adding a serum albumin aqueous solution, stirring and mixing, adding a polyethylene glycol aqueous solution, stirring and mixing, adding a cocamidopropyl betaine aqueous solution, stirring and mixing, and separating out a product. The colloidal carbon labeled antibody prepared by the method can improve the detection sensitivity of a colloidal carbon immunochromatography technology on the bladder tumor antigen, and is beneficial to improving the detection accuracy and reducing the possibility of leak detection while realizing the rapid and convenient detection of the bladder tumor antigen.

The application discloses a kind of phenylboronic acid and dopamine grafted chitosan-manganese-based injectable hydrogel immunoadjuvant and its preparation method and application.The hydrogel takes chitosan as base material, constructs modified polymer by chemical grafting dopamine and phenylboronic acid;Manganese ion is loaded to form phenylboronic acid and dopamine grafted chitosan-manganese-based injectable hydrogel immunoadjuvant.The hydrogel is fixed antigen by physical adsorption, has the functions of targeted delivery, immune activation and controllable release, significantly enhances humoral immunity and cellular immunity.The hydrogel has the characteristics of low viscosity, supports simple injection, biodegradable and no local inflammatory reaction.Experiments show that the titer of the adjuvant is equivalent to that of Freund's adjuvant after immunizing mice, successfully used for preparing monoclonal antibody of gladiolus burkholderia, and constructs immune test strip, and the sensitivity reaches 5×10 5 CFU / mL.The application solves the problems of high toxicity, local damage and high cost of traditional adjuvant, and is suitable for vaccine development and immunodiagnosis.

The invention discloses a preparation method and application of a monoclonal antibody of PBP2a, and belongs to the technical field of immunodiagnosis. The monoclonal antibody has the advantage that the comprehensive performance is remarkably improved, and the core is that the anti-PBP2a monoclonal antibody with higher affinity (target gt and 109 M-1) and higher specificity is obtained through optimized antigen design and immune strategy. The detection kit constructed based on the antibody aims to realize a lower detection limit (less than or equal to 103 CFU / mL) and a higher detection speed (5-10 minutes), and provides a'sample-splitting-sample adding 'integrated operation process to improve convenience. Meanwhile, through strict stability testing, the antibody and the kit are ensured to have longer storage periods, so that the clinical requirements on rapid, accurate, convenient and stable MRSA immunodiagnosis are comprehensively met.

The application relates to the field of immunodiagnostic materials, in particular to an amphoteric multi-arm polyethylene glycol derivative of a compound shown in the following general formula and application thereof. The compound provided by the application can block the non-specific adsorption of interfering substances in a determination system, protects the surface of a solid-phase carrier, and can be used in practice as a low-cost substitute for a protein blocking agent.

The invention discloses an antibody, an antibody pair, a reagent and a method for detecting glial fibrillary acidic protein, and relates to the field of immunodiagnosis. The antibody pair for detecting the glial fibrillary acidic protein comprises the first antibody and the second antibody, and the reagent and the detection method based on the antibody and the antibody pair can accurately detect the existence of the glial fibrillary acidic protein.

The application discloses a kind of detection anti-Brucella antibody multi-link B cell epitope recombinant protein and its application, it belongs to the field of biotechnology and immunodiagnosis technique, wherein, multi-link B cell epitope recombinant protein is formed by the B cell epitope of seven key outer membrane proteins Omp10, Omp16, Omp19, Omp25, Omp31, Omp2b, BP26 of Brucella, its amino acid sequence is as shown in sequence table SEQ ID NO:1.The multi-link B cell epitope recombinant protein has good antigenicity and reactivity, and the deer serum indirect ELISA detection system established with it as coating antigen shows excellent detection performance, has high sensitivity, strong specificity and good repeatability, and can accurately detect the level of anti-Brucella antibody in deer serum sample.

Provided are a recombinant antigen, a kit containing same, and a use, belonging to the technical field of immunodiagnostics. Specifically, the present invention relates to a recombinant antigen containing rraB or MysB as a fusion partner, a recombinant nucleic acid molecule, a recombinant expression vector, a recombinant host cell, a labeling conjugate, a kit, and a use. Using the recombinant antigen for immunodiagnosis can significantly improve the activity and specificity of immunodiagnosis.

The invention provides an immunodiagnostic reagent accelerant and a preparation method thereof. Comprising a reinforcing agent, a pH regulator, protective protein, a surfactant, inorganic salt and a bacteriostatic agent. The immunodiagnostic reagent accelerant is used as a matched enhancing reagent of a common immunodiagnostic reagent, the binding efficiency of an antigen and an antibody in the immunodiagnostic reagent can be effectively improved, and a detection signal is increased by improving the binding efficiency, so that the improvement of the detection sensitivity and the reduction of the detection time are realized; the detection efficiency is improved.