91 results about "Immunodiagnostics" patented technology

The invention relates to the field of in-vitro immunodiagnosis detection, in particular to an IgG antibody, an IgM antibody and a method. The invention provides an IgG (immunoglobulin G) antibody specifically bound with coxsackie virus, which is characterized in that amino acid sequences of complementary determining regions CDRH1-CDRH3 of a heavy chain variable region of the IgG antibody are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; the amino acid sequences of the complementary determining regions CDRL1-CDRL3 of the light chain variable region are respectively as shown in SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6. The IgG antibody provided by the invention not only has good specificity and can specifically recognize the coxsackie virus, but also has high sensitivity and stability.

The invention discloses an antibody pair, a reagent and a method for detecting HIV P24, and relates to the field of immunodiagnosis. The antibody pair for detecting the HIV P24 disclosed by the invention comprises the first antibody and the second antibody. The reagent and the detection method based on the antibody pair can accurately detect the existence of the HIV P24.

The invention relates to the technical field of immunodetection and immunodiagnosis, in particular to a chemiluminescence substrate solution, a chemiluminescence detection kit and a detection method. The chemiluminescent substrate solution comprises a substrate solution 1, or comprises the substrate solution 1 and a substrate solution 2, the substrate solution 1 comprises sodium hydroxide, a surfactant and a blue light absorbent for absorbing blue light with the wavelength of 400-500 nm; the substrate solution 2 comprises inorganic acid and hydrogen peroxide. According to the application, the blue light absorbent is added into the chemiluminescent substrate liquid, so that blue light emitted by acridinium ester can be absorbed, a luminescent signal is reduced, and particularly, the luminescent value of a background caused by non-specific adsorption is reduced, so that the signal-to-noise ratio is increased.

The invention relates to the technical fields of gene engineering, antibody engineering and immunodiagnosis. The invention provides a recombinant humanized anti-Cpn IgM monoclonal antibody. The monoclonal antibody comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are respectively shown as SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5; the heavy chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are shown as SEQ ID NO.6, SEQ ID NO.7 and SEQ ID NO.8 respectively. The invention also provides a method for preparing the monoclonal antibody. The recombinant humanized anti-Cpn IgM monoclonal antibody provided by the invention is expressed in mammalian cells by utilizing a gene recombination technology, has the characteristics of high purity, small batch difference and the like, and can be applied to a Cpn IgM antibody detection kit as a quality control product; as a quality control product, the recombinant humanized anti-Cpn IgM monoclonal antibody has the advantages of high sensitivity, high safety, inter-batch stability and the like.

The invention belongs to the technical field of antibodies, and particularly relates to an antibody and an antibody pair for resisting human IL-12 / IL-23p40 protein, and application of the antibody and the antibody pair. The antibody is a first antibody or a second antibody, amino acid sequences of light chains CDR1-3 of the first antibody are respectively shown as SEQ ID NO.3-5, and amino acid sequences of heavy chains CDR1-3 of the first antibody are respectively shown as SEQ ID NO.8-10; the amino acid sequences of light chains CDR1-3 of the second antibody are respectively as shown in SEQ ID NO.13-15, and the amino acid sequences of heavy chains CDR1-3 of the second antibody are respectively as shown in SEQ ID NO.18-20. The two antibodies disclosed by the invention have specific recognition and high-affinity binding capacities on recombinant and natural human IL-12 / IL-23p40 protein, and have important practical values in the fields of immunodiagnosis and immunotherapy taking p40 as a target spot.

The invention discloses a recombinant protein for detecting an anti-myelin sheath related glycoprotein MAG antibody and a detection reagent of the recombinant protein, and belongs to the technical field of neural immunodiagnostics, five amino acid sites in MAG protein are subjected to specific amino acid point mutation replacement to obtain an MAG protein mutation sequence, the MAG protein mutation sequence is fused with GlcATP enzyme and HNK-1ST enzyme full-length sequences to construct a lentiviral expression vector, and the recombinant protein and the detection reagent are used for detecting the anti-myelin sheath related glycoprotein MAG antibody. According to the present invention, three genes are constructed, and are stably transfected in a host cell so as to stably express the three genes in one cell at the same time, such that the constructed recombinant protein and the wild type MAG protein have similar anti-MAG IgM antibody binding domain, and on the premise of not using GlcATP enzyme and HNK-1ST enzyme small molecule agonists, the sensitivity and the specificity of the detection of the MAG autoimmune antibody by using the CBA method are improved; the method is suitable for preparing related detection reagents for in-vitro detection of the IgM type anti-MAG antibody.

The invention discloses an antibody pair, a reagent and a method for detecting soluble growth stimulation expression gene 2 protein, and relates to the field of immunodiagnosis. The antibody pair for detecting the soluble growth stimulation expression gene 2 protein comprises the first antibody and the second antibody, and the reagent and the detection method based on the antibody pair can accurately detect the existence of the soluble growth stimulation expression gene 2 protein.

The invention belongs to the technical field of in-vitro immunodiagnosis, and particularly relates to a test strip for combined detection of AMH and FSH and a preparation method thereof.The test strip comprises a pretreated combination pad, a pretreated sample pad and a nitrocellulose membrane, and the nitrocellulose membrane is coated with an antibody; the preparation method of the test strip comprises the following steps: pretreating the conjugate pad with a conjugate pad pretreatment solution, drying, and spraying a fluorescent microsphere labeled antibody mixed solution; pretreating a sample pad with a sample pad pretreatment solution, and drying for later use; diluting the antibody by adopting a coating antibody diluent, marking on a nitrocellulose membrane, and drying for later use after marking is finished; the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are sequentially pasted to a PVC bottom plate, and the test strip is cut. The test strip can jointly detect AMH and FSH indexes, is easy to operate, small in harm to the human body, economical and applicable, comprehensively detects multiple ovarian reserve function biological indexes, can more comprehensively and accurately evaluate the female fertility, and has good application prospects. The actual clinical requirements are met.

The invention belongs to the technical field of immunodiagnosis, and relates to a detection card for quantitatively detecting human total IgE antibodies, which comprises a card shell and a test strip, and the test strip is arranged in the card shell; the test strip comprises a sample pad, a combination pad, a reaction film and an absorption pad which are arranged on a bottom plate, wherein an animal anti-human IgE monoclonal antibody marked by lanthanide fluorescent microspheres is arranged on the combination pad; a detection line coated with an animal anti-human IgE polyclonal antibody and a quality control line coated with an animal IgG antibody are arranged on the reaction film. The invention further provides a preparation method, a kit and application of the detection card for quantitatively detecting the human total IgE antibody. The method can effectively eliminate the interference of non-specific fluorescence, greatly improves the analysis sensitivity, and is simple and convenient to operate and low in cost.

The invention discloses an antibody pair, a reagent and a method for detecting influenza A virus, and relates to the field of immunodiagnosis. The antibody pair for detecting the influenza A virus comprises the first antibody and the second antibody, and the reagent and the detection method based on the antibody combination can accurately detect the existence of the influenza A virus.

The present disclosure relates to a diagnostic cartridge for immunodiagnosis, and a reader and a diagnostic system using the same. A diagnostic cartridge, including the diagnostic pad and the color reaction pad, is provided to detect a color reaction and a fluorescence reaction through the single diagnostic cartridge.

The invention provides an anti-RBD immunomagnetic bead as well as a preparation method and application thereof, and belongs to the technical field of immunodiagnosis. The preparation method of the anti-RBD immunomagnetic beads comprises the following steps: washing aminated magnetic beads with a 1 * PBST solution, and removing supernatant after washing; adding 15% glutaraldehyde, activating in a dark place at room temperature, removing the supernatant after activation, adding 1 * PBST solution to wash the magnetic beads, and removing the supernatant after washing; adding a 28D9 antibody, incubating at room temperature in a dark place, and removing supernatant after incubation is finished; adding a 1 * PBST solution containing 5% of BSA (Bovine Serum Albumin) for sealing at room temperature in a dark place, removing supernatant after sealing is finished, washing the magnetic beads by using the 1 * PBST solution, and removing supernatant after washing is finished; and adding a preserving fluid to obtain the anti-RBD immunomagnetic bead. The anti-RBD immunomagnetic bead provided by the invention can be used for rapidly purifying RBD protein, so that the time is greatly saved for the production of new crown protein vaccines.

The invention discloses an antibody pair, a reagent and a method for detecting helicobacter pylori, and relates to the field of immunodiagnosis. The antibody pair for detecting the helicobacter pylori comprises the first antibody and the second antibody, and the reagent and the detection method based on the antibody pair can accurately detect the existence of the helicobacter pylori.

The invention discloses an antibody for detecting RhD blood group antigen and application thereof, and relates to the technical field of immunodiagnosis. The anti-RhD blood group antigen antibody disclosed by the invention comprises a heavy chain complementarity determining region and a light chain complementarity determining region, provides an important raw material source for detection of the RhD blood group antigen, and has improved low-temperature stability.

The present invention relates to the field of antibodies, antigen-binding fragments and immunodiagnosis. Specifically, the present invention relates to recombinant antibodies or antigen-binding fragments for binding thyroid hormone T4 (thyroxine) and halogenated bisphenol A. Furthermore, the present invention relates to isolated nucleic acid molecules comprising nucleotide sequences encoding the recombinant antibodies or antigen-binding fragments of the present invention, as well as expression vectors and host cells comprising the nucleic acid molecules of the present invention. In addition, the present invention relates to methods for producing recombinant antibodies or antigen-binding fragments for binding T4 thyroid hormone and halogenated bisphenol A, test kits and immunoassays comprising the recombinant antibodies or antigen-binding fragments of the present invention, and methods for determining the levels of T4 thyroid hormone and / or halogenated bisphenol A in a sample of a subject. Furthermore, the present invention relates to methods for processing samples, such as immunoaffinity-based sample preparation methods for enriching halogenated bisphenol A from a sample.

The present invention relates to an immune device for discriminating whether a pregnancy or an abnormal pregnancy occurs, and more particularly, to an immune device comprising a novel multi-binding anti-hCG monoclonal antibody capable of jointly recognizing a complete hCG and a beta cf hCG, comprising two detection lines and a quality control line capable of separately detecting the complete hCG and the beta cf hCG contained in a specimen, according to the present invention, it is possible to accurately and quickly distinguish the number of weeks of pregnancy, normal pregnancy, including extrauterine pregnancy, and abnormal pregnancy of a withered egg by numeralizing the ratio of the color development intensity of beta cf hCG (the distribution ratio of beta cf hCG) with respect to the color development intensity of complete hCG and beta cf hCG.

The invention discloses an antibody pair, a reagent and a method for detecting malaria, and relates to the field of immunodiagnosis. The antibody pair for detecting malaria disclosed by the invention comprises the first antibody and the second antibody, and the reagent and the detection method based on the antibody pair can accurately detect the existence of malaria.

This invention relates to a high-titer prealbumin antibody purification method and its application, belonging to the field of biotechnology. The method utilizes Protein A affinity chromatography to capture total IgG from antiserum, followed by purification using an affinity chromatography column prepared by targeted conjugation of a specific sequence peptide. The antibody purified by this method achieves a titer of 1:256,000 in ELISA detection, with a half-maximal effective concentration (EC50). 50 The antibody concentration was 3.2 ng / mL, exhibiting extremely high affinity and reactivity. When used in a double-antibody sandwich chemiluminescent immunoassay, it achieved a detection limit of 0.08 µg / mL, a wide dynamic range (0.08–400 µg / mL), and a titer retention rate as high as 88% after accelerated storage at 37°C for 14 days, demonstrating significantly better stability than antibodies prepared by traditional methods. This invention provides a highly active and stable core antibody raw material for the preparation of high-performance immunodiagnostic reagents.

The invention discloses an antibody pair, a reagent and a method for detecting CA15-3, and relates to the field of immunodiagnosis. The antibody pair for detecting the CA15-3 disclosed by the invention comprises the first antibody and the second antibody, and the reagent and the detection method based on the antibody combination can accurately detect the existence of the CA15-3.

The present invention discloses a recombinant protein for detecting anti-myelin associated glycoprotein (MAG) antibody and a detection reagent thereof, belonging to the technical field of neuro-immunodiagnosis. By performing specific amino acid point mutation substitutions at five amino acid positions in the MAG protein, a MAG protein mutant sequence is obtained, and then it is fused with the full-length sequences of GlcATPase and HNK-1ST enzyme to construct a lentiviral expression vector, which is stably transfected into host cells, enabling the simultaneous stable expression of the three genes in one cell. The constructed recombinant protein has a similar anti-MAG IgM antibody binding domain to the wild-type MAG protein, and without using small molecule agonists of GlcATPase and HNK-1ST enzyme, it improves the sensitivity and specificity of detecting MAG autoantibodies by the CBA method, and is suitable for preparing related detection reagents for in vitro detection of IgM-type anti-MAG antibodies.

The invention discloses an antibody pair, a reagent and a method for detecting malaria, and relates to the field of immunodiagnosis. The antibody pair for detecting malaria disclosed by the invention comprises the first antibody and the second antibody, and the reagent and the detection method based on the antibody combination can accurately detect the existence of malaria.

The invention discloses a small molecule hapten detection reagent and a detection method, and relates to the technical field of immunodiagnosis. The detection reagent disclosed by the invention comprises a small-molecule hapten, an anti-small-molecule hapten antibody, a compound antibody and a signal marker, the detection method includes detecting at least a first signal and a second signal. According to the small-molecule hapten detection scheme, a more accurate detection reagent is provided for small-molecule hapten detection, and meanwhile, a novel more accurate detection method is provided for small-molecule hapten detection.

The invention discloses an antibody pair, a reagent and a method for detecting respiratory syncytial virus, and relates to the field of immunodiagnosis. The antibody pair for detecting the respiratory syncytial virus comprises the first antibody and the second antibody, and the reagent and the detection method based on the antibody pair can accurately detect the existence of the respiratory syncytial virus.

The invention belongs to the technical field of medical immunodiagnostic reagents, and discloses a freeze-dried VB12 rapid detection kit and a VB12 detection method. According to the kit provided by the invention, in the preparation process of a freeze-dried microsphere detection reagent, trehalose, glucose, gelatin, triton, polyethylene glycol, Proclin 950, 5-bromo-5-nitro-1, 3-dioxane, mannitol and glycine in a specific mass ratio are adopted as a freeze-drying solution system; stress damage to VB12 binding protein and a VB12 compound antibody in the freeze-drying process is reduced, the stability of the reagent in the preparation and storage process is improved, meanwhile, quick redissolution of the freeze-drying reagent can be achieved, and a detection system formed by compounding a reagent storage solution, a freeze-drying microsphere redissolution solution and a sample dissociation solution has the excellent anti-interference capacity and can be used for detecting the VB12 binding protein and the VB12 compound antibody in the freeze-drying process. The obtained detection kit has the advantages of excellent detection sensitivity, reliability, stability and the like, and has a very excellent practical application prospect.

The invention discloses an antibody pair, a reagent and a method for detecting NT-proBNP, and relates to the field of immunodiagnosis. The antibody pair for detecting the NT-proBNP disclosed by the invention comprises the first antibody and the second antibody. The reagent and the detection method based on the antibody pair can accurately detect the existence of the NT-proBNP.

The invention discloses an antibody pair, a reagent and a method for detecting malaria, and relates to the field of immunodiagnosis. The antibody pair for detecting malaria disclosed by the invention comprises the first antibody and the second antibody, and the reagent and the detection method based on the antibody pair can accurately detect the existence of malaria.

The present disclosure relates to an immunodiagnostic device for determining normal and abnormal pregnancy, and more particularly, to an immunodiagnostic device including a multi-binding anti-hCG monoclonal antibody that may recognize Intact hCG and βcf hCG in common, wherein Intact hCG and βcf hCG contained in the sample may be separately detected, including two detection lines and control lines, and the ratio of the color development intensity of βcf hCG to the color development intensity of Intact hCG and βcf hCG (the distribution ratio of βcf hCG) may be numerically quantified to determine the number of weeks of pregnancy, normal pregnancy, and abnormal pregnancy including ectopic pregnancy and blighted ovum accurately and quickly.

The invention discloses a recombinant peanut allergen fusion protein and application thereof, the amino acid sequence of the recombinant peanut allergen fusion protein is as shown in SEQ ID NO.1, and the corresponding coding nucleotide sequence of the recombinant peanut allergen fusion protein is as shown in SEQ ID NO.2. The recombinant peanut allergen fusion protein comprises an Ara h 8 protein 1-157aa sequence fragment, an Ara h 9 protein 1-116aa sequence fragment and an Ara h 2 protein 22-172aa sequence fragment, the three proteins are sequentially connected through a Linker sequence to obtain the peanut allergen fusion protein, and a recombinant fusion expression method of the fusion protein and a detection kit based on the protein are provided. The fusion protein and the kit thereof can be applied to peanut desensitization treatment, can also improve the positive detection rate in immunodiagnosis application and eliminate cross interference caused by homologous sequences of other allergens, and can improve the performance of a peanut allergy diagnostic reagent as a raw material.

The present invention provides a dual immunodiagnostic chip. The dual immunodiagnostic chip can be used to observe changes in color and electrical signal, through a single sample injection. Thus, the presence or absence of a target material can be rapidly and accurately detected. The dual immunodiagnostic chip of the present invention comprises: an injection unit (100) into which a composite nanostructure in which a sample, nanoparticles, and a primary bioreceptor are conjugated; a reaction unit (200) coated with a chromogenic reagent that develops color by reacting with the composite nanostructure; an electrode unit (300) on which a secondary bioreceptor capable of binding to a target material is immobilized and which generates an electrical signal; and a microfluidic channel unit (400) which is connected to the injection unit (100), the reaction unit (200), and the electrode unit (300) and provides a passage through which the composite nanostructure injected into the injection unit (100) can flow into the reaction unit (200) and the electrode unit (300).

The invention discloses a vitamin B12 detection kit and a preparation method thereof, and relates to the technical field of immunodiagnosis, the vitamin B12 detection kit comprises a releasing agent solution, a neutralizer solution, a solid phase coating reagent solution, a liquid phase labeling reagent solution and a transcobalamin antibody solution. The releasing agent solution comprises a releasing agent, and the releasing agent comprises at least one of sodium hydroxide and potassium hydroxide; the solid-phase coating reagent solution comprises a goat anti-mouse IgG coated with carboxyl magnetic beads and a mouse vitamin B12 monoclonal antibody compound; the liquid phase labeling reagent solution comprises vitamin B12-BSA labeled by acridinium ester. According to the invention, the transcobalamin site obtained after the vitamin B12-transcobalamin compound is released is closed by using the transcobalamin antibody, so that the vitamin B12 and the transcobalamin are prevented from forming a compound again, and the value measurement accuracy of the vitamin B12 detection kit is improved.