Anti-vibrio parahaemolyticus chicken yolk antibody, preparation method and application thereof

Abstract

The invention discloses anti-vibrio parahaemolyticus chicken egg yolk antibody and the preparation method. The anti-vibrio parahaemolyticus chicken egg yolk antibody is prepared and obtained through the steps of preparation and inactivation of vibrio parahaemolyticus antigen, immunization of hens, collection of immunized eggs, coarse extraction of anti-vibrio parahaemolyticus chicken egg yolk antibody from the egg yolk liquid of the immunized eggs and purification. The invention also provides the application of the anti-vibrio parahaemolyticus chicken egg yolk antibody in food safety detection reagents and disease immune diagnostic reagents related with the preparation of vibrio parahaemolyticus and medicines and healthy products or feed additives for preventing and curing the related diseases of vibrio parahaemolyticus. The anti-vibrio parahaemolyticus chicken egg yolk antibody (IgY) prepared and provided by the invention has the advantages that the specificity and the purity are high, the anti-vibrio parahaemolyticus chicken egg yolk antibody has good effects when being used for preparing immunology testing reagents and medicines and feed additives for treating the related diseases of vibrio parahaemolyticus, the cost is low, the production volume is high, and the anti-vibrio parahaemolyticus chicken egg yolk antibody is easy to be industrialized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation and purification method and application of an egg yolk antibody product with antibody activity—chicken egg yolk antibody against Vibrio parahaemolyticus. Background technique [0002] Vibrio parahaemolyticus is a halophilic bacterium widely distributed in oceans and salt lakes. It is one of the most common pathogens of food poisoning in summer. In recent years, foodborne diseases caused by Vibrio parahaemolyticus have always It is the primary cause of primary diseases and seriously threatens people's health. At the same time, vibriosis is also one of the common diseases in mariculture industry. Vibrio parahaemolyticus can infect many kinds of fish and shrimps and cause a large number of deaths, resulting in huge economic losses. In recent years, the intensive culture of seawater fish and shrimp, especially cage culture has become the main culture mo...

AI Technical Summary

Problems solved by technology

TDH gene and TRH gene are not single genes, among them, the homology of TDH gene family reaches more than 80%, while the homology of TRH gene and TDH gene is 68%, so a pair of primers cannot detect all TDH and TRH genes

Some scholars found in the study of cases that some pathogenic Vibrio parahaemolyticus urease were positive while KP - , and believed that urease was the main cause of the disease, but related research results showed that most of the urease-positive strains carried TDH and TRH at the same time, so the synergistic effect of TDH and TRH in the pathogenesis of urease-positive Vibrio parahaemolyticus cannot be ruled out

Studies have shown that Vibrio parahaemolyticus in non-clinical samples has only 1% to 2% Kp + parahaemolyticus, but because the toxin-producing conditions of Vibrio parahaemolyticus will be affected by such as the environment of the digestive tract or food matrix, the current health standard does not only use KP as the basis for judgment, but requires that the detection of Vibrio parahaemolyticus should not be detected bacteria

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