A peroxynitrite anion fluorescent probe with AIE effect and a synthesis method and application thereof

Abstract

This invention discloses a peroxynitroso anion fluorescent probe with AIE effect, its synthesis method, and its applications. The near-infrared fluorescent probe TPS was designed and synthesized in this invention. It is nearly colorless under ultraviolet light irradiation but emits bright yellow fluorescence upon reaction with peroxynitroso anions. Detection showed that TPS reacts with ONOO in a HEPES:acetonitrile = 1:1 system. ‑ The response time was 100 min, after adding 120 uL of 0.5×10 ‑3 mol·L ‑1 ONOO ‑ The reaction is complete. TPS exhibits high specificity, reacting only with peroxynitrosoanion among various anions, demonstrating high sensitivity. TPS reacts with ONOO at pH 4-8. ‑ The ion response exhibits good stability, and TPS is effective against ONOO. ‑ The response exhibits good linearity, with a detection limit of 0.08232. µmol·L ‑1 Probe TPS and ONOO ‑ The reaction, after different concentrations of THF, exhibited a typical AIE effect.

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