1067 results about "Magnetic bead" patented technology

The invention relates to the technical field of nano magnetic bead preparation, and discloses a magnetic bead continuous separation device. The magnetic bead continuous separation device comprises a magnetic bead separator, a magnetic field generating device and a driving device, the driving device is used for driving the magnetic field generating device to be close to or away from the side wall of the magnetic bead separator, the magnetic bead separator is hollow, one end of the magnetic bead separator is provided with a first liquid inlet and a second liquid inlet, and the other end of the magnetic bead separator is provided with a first liquid outlet and a second liquid outlet. According to the invention, a plurality of separation processes can be integrated in the same structure, so that the system configuration is simplified, and the requirements of automation and large-scale continuous treatment in the magnetic bead purification process are effectively met.

The multifunctional clamping jaw is installed at the tail end of the mechanical arm through a rotary connecting plate and comprises a clamping jaw body, a first clamping jaw body and a second clamping jaw body, and the clamping jaw body is fixedly connected with the rotary connecting plate and can rotate in the Z-axis direction; the first clamping jaw is arranged on the clamping jaw body and is used for clamping a kit; the second clamping jaw is arranged on the clamping jaw body and used for clamping a magnetic bar sleeve. The utility model provides the multifunctional clamping jaw which is designed according to the magnetic bead purification requirement and can be used for compatibly clamping a kit and a magnetic bar sleeve material, the automatic carrying of the magnetic bead purification material can be realized by mounting the multifunctional clamping jaw at the tail end of a mechanical arm, the automatic carrying and placing of the kit and the magnetic bar sleeve required by the magnetic bead purification can be realized by the multifunctional clamping jaw, and the synchronous carrying can be realized; and meanwhile, materials of multiple specifications are compatible with the same set of robot, and the cost is greatly saved.

The invention relates to a method for improving chemiluminescence immunoassay sensitivity of magnetic particles, a detection kit and application thereof, firstly, magnetic beads are coupled with an antibody, then magnetic beads are sealed to obtain a magnetic bead coated antibody, sealing comprises pre-sealing and second-step sealing, glycine and ethanolamine are added in the pre-sealing process, and in the second-step sealing process, glycine and ethanolamine are added in the second-step sealing process. Casein hydrolysate and dodecyl dimethyl amine ethyl lactone are added; then coupling the antibody with alkaline phosphatase or streptavidin to obtain an alkaline phosphatase labeled antibody or a streptavidin labeled antibody; the magnetic bead coated antibody, the alkaline phosphatase labeled antibody or the streptavidin labeled antibody is used for sample detection. The method is high in sensitivity and wide in detection range, is adaptive to a common immunoassay instrument, does not need expensive equipment, and is also simple to operate; in addition, the AMH concentration with extremely low concentration can be accurately detected.

The invention discloses a fruit and vegetable exosome extraction method based on ligand fishing. The fruit and vegetable exosome extraction method comprises the following steps: suspending amino-functionalized Fe3O4 (at) SiO2 nanoparticles in a glutaraldehyde solution, and adding activated agglutinin to obtain Fe3O4 (at) SiO2-agglutinin nanoparticles; adding a glutaraldehyde solution into agarose gel to obtain a size exclusion chromatographic column filler; the method comprises the following steps: dispersing Fe3O4 (at) SiO2-lectin nanoparticles in a PBS buffer solution to obtain a uniform suspension, adding the uniform suspension into a size exclusion chromatographic column filler to obtain a uniform slurry, filling the size exclusion chromatographic column with the uniform slurry, and flushing with the buffer solution to form a magnetic bead stationary phase; and loading fruit and vegetable supernatant into a size exclusion chromatographic column, passing through the magnetic bead stationary phase, cleaning residual impurities combined on the exosome-magnetic bead compound by using a buffer solution, and dissociating the exosome from the magnetic beads by using a competitive eluent of the exosome to obtain the high-purity fruit and vegetable exosome. The problem that an existing exosome extraction method is difficult to meet the requirements of high efficiency, simplicity, high purity and high activity at the same time is solved.

The invention relates to an HBV magnetic bead as well as a preparation method and application thereof. The HBV magnetic beads are HBV magnetic beads with molecular tag primer sequences, poly (dT) and N HBV specific capture probes are coupled and modified, and N is a positive integer and is greater than or equal to 1. The HBV specific capture probe is selected from the following groups: (a) a pgRNA probe; (b) an S probe; (c) an X probe; (d) an rcDNA probe; (e) a cccDNA probe; wherein the pgRNA probe targets a positive chain pgRNA region of the HBV, the S probe targets a positive chain S region of the HBV, the X probe targets a positive chain X region of the HBV, the rcDNA probe targets a negative chain rcDNA region of the HBV, and the cccDNA probe targets a negative chain cccDNA region of the HBV. By adopting the novel HBV magnetic bead, the enrichment of HBV sequences can be greatly improved.

The utility model provides a magnetic frame, and relates to the technical field of biology and medical treatment. The magnetic frame comprises a magnetic frame body and a magnetic fixing piece, a placing space is arranged on one side of the magnetic frame body, and one end, away from the magnetic frame body, of the placing space is open; the two ends of the magnetic fixing piece are arranged on the left side and the right side of the opening of the containing space respectively. According to the magnetic frame, an existing magnetic frame structure is thoroughly changed, and one side of the magnetic frame body is provided with the containing space used for containing the magnetic bead incubation structure and the magnetic fixing belt used for fixing the magnetic bead incubation structure in the containing space, so that the magnetic bead incubation structure of a non-centrifugal tube is fixed.

The utility model belongs to the field of microorganism extraction equipment, and particularly relates to a microorganism extraction system. Comprising a microorganism extraction container, the microorganism extraction container contains a to-be-extracted solution and magnetic beads for adsorbing microorganisms, and the to-be-extracted solution contains the microorganisms to be extracted; the workbench is provided with a container placing part for placing the microorganism extraction container; the magnetic bead adsorption mechanism comprises a magnetic attraction assembly and a magnetic attraction state switching assembly used for changing the magnetic attraction state, and the magnetic attraction assembly has magnetism and is used for adsorbing magnetic beads located in the microorganism extraction container; and the controller is electrically connected with the magnetic attraction state switching assembly and is used for changing the state of the magnetic attraction assembly or changing the relative state of the magnetic attraction assembly and the microorganism extraction container. The operation is simple and convenient, the automatic operation of microorganism extraction is favorably realized, the difficulty of microorganism extraction is reduced, and the efficiency of microorganism extraction is improved.

The invention relates to the technical field of nano magnetic bead preparation, and discloses a magnetic bead continuous separation system and a control method. The system comprises a magnetic bead separation device which is of a hollow pipe structure, the pipe wall of the magnetic bead separation device can be magnetized and demagnetized, one end of the magnetic bead separation device is provided with a first liquid inlet and a second liquid inlet, and the other end of the magnetic bead separation device is provided with a first liquid outlet and a second liquid outlet; the stock solution tank is connected to the first liquid inlet through a first flow channel; the waste liquid tank is connected to the first liquid outlet through a fifth flow channel, and a first magnetic bead sensor is arranged on the fifth flow channel; a liquid inlet of the product tank is connected to the second liquid outlet through a sixth flow channel, and a second magnetic bead sensor is arranged on the sixth flow channel; a liquid inlet of the stirring kettle is connected to the second liquid outlet through a seventh flow channel, and a liquid outlet of the stirring kettle is connected to the first liquid inlet through a second flow channel; a liquid outlet of the washing liquid tank is connected to the first liquid inlet through a third flow channel and is connected to the second liquid inlet through a fourth flow channel. The system has the advantage of meeting the requirements of continuous, automatic and infinite batch separation and purification of the magnetic beads.

The invention relates to the technical field of new material application, and discloses a magnetic bead material for enriching a sulfydryl peptide fragment and a preparation method thereof.The magnetic bead material prepared from micron carboxyl magnetic beads, dodecane dicarboxylic acid dihydrazide and SPSP has a long chemical reaction connecting arm, so that a reaction group has the better freedom degree, the reaction with sulfydryl is better facilitated, and the magnetic bead material can be used for enriching the sulfydryl peptide fragment. The enrichment efficiency is relatively high. When the magnetic bead material is prepared, micron carboxyl magnetic beads and dodecane dicarboxylic acid dihydrazide are based on hydrazine group and carboxyl condensation, the condensation reaction is more selective and more stable, the reaction is carried out under a milder condition, the reaction product is stable, and the reaction efficiency is higher at a lower reaction temperature. Dodecane dicarboxylic acid dihydrazide and SPSP are based on a reaction of N-succinimide and hydrazine, N-succinimide has high specificity to amino-containing molecules, side reactions are reduced, the reaction is generally carried out at room temperature, strong acid and alkali conditions are not needed, and protection of other sensitive groups is facilitated.

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The invention discloses a magnetic bead continuous separation process and device, and the process comprises the following steps: firstly applying a magnetic field to a magnetic bead separator, then continuously adding a stock solution into the magnetic bead separator, forming a magnetic bead capturing area and a magnetic bead separation area under the action of the magnetic field, capturing magnetic beads to the capturing area, discharging impurities to a waste liquid tank from a liquid outlet, and separating the impurities from the waste liquid tank. The concentration of the magnetic beads at the liquid outlet exceeds a set value; after the magnetic field is relieved, washing liquid is continuously added into the magnetic bead separator, and the magnetic beads are flushed out and collected into an intermediate tank; applying the magnetic field again, and continuously adding the collected liquid into the magnetic bead separator for separation again; after the magnetic field is relieved, washing liquid is injected into an inlet of the magnetic bead capturing area, so that the magnetic beads are flushed out until the concentration of the magnetic beads at a liquid outlet is lower than a set value; and circulating the steps until the treatment is finished. The process is automatically operated through a PLC (Programmable Logic Controller) control system. The magnetic bead separation device has the advantages that automatic feeding and discharging are achieved, multiple operation steps are completed in the same device, and multi-round repeated separation and automatic completion of magnetic beads are achieved.

The invention provides an immunomagnetic bead, a preparation method and a detection reagent. The preparation method comprises the following steps: preparing a modified magnetic bead containing a dibenzocyclooctyne group; protein and a coupling agent containing azide groups are sequentially dissolved in a second coupling buffer solution to react, modified protein containing azide groups is obtained, the final concentration of the reacted protein is 0.5-5 mg / mL, and the final concentration of the coupling agent is 0.05-5 mM; and mixing the modified magnetic beads and the modified protein according to an addition ratio of 1mg: (1-40) nmol, and reacting to obtain the immunomagnetic beads. According to the present invention, the immunomagnetic bead preparation method has advantages of high coupling efficiency, small inter-batch difference, strong specificity and difficult aggregation during the preparation process, such that the accurate detection of the antigen concentration is achieved so as to solve the defects of low coupling efficiency, large inter-batch difference and easy aggregation existing in the immunomagnetic bead preparation method in the related technology.

The invention relates to the technical field of life science, in particular to a full-automatic nucleic acid extraction system and process, and the system comprises a complete machine frame, a sample adding and waste liquid treatment mechanism, an oscillation magnetic suction heating mechanism, a control system and a screen interaction system. The sample adding and waste liquid treatment mechanism realizes three-dimensional accurate positioning through transverse and longitudinal movement guide rails and a vertical movement assembly, comprises a large piston device and a small piston device which are connected in parallel, and can flexibly switch different volumes of pipettes and share a connector; the oscillation magnetic suction heating mechanism integrates oscillation, heating and magnetic suction functions and is matched with multi-specification suction heads and kits to complete sample treatment. According to the extraction process, all the mechanisms are linked through a control system, and full-flow operation such as sample cracking, solid-liquid separation, magnetic bead purification and elution is automatically completed. The nucleic acid extraction device realizes automation and precision of nucleic acid extraction, improves the operation efficiency and the extraction effect, and is suitable for nucleic acid extraction of samples such as plant tissues, animal tissues, blood and plasmids.

The invention relates to the technical field of medical detection, in particular to a method for detecting aldosterone, cortisol, deoxycorticosterone and cortisone in plasma through LC-MS / MS. The method comprises the following steps: (a) mixing a plasma sample with an internal standard solution to obtain a mixed solution; (b) adding the mixed solution into the magnetic bead suspension, sequentially carrying out adsorption, weak washing, strong washing and elution, and collecting an eluent; (c) carrying out liquid nitrogen blowing and redissolving on the eluent, and then carrying out LC-MS / MS detection; wherein the surfaces of the magnetic beads are modified with steroid adsorption groups, and the target detection objects are aldosterone, cortisol, deoxycorticosterone and cortisone. The detection method is convenient and simple, the detection efficiency is higher than that of a traditional method, and a feasible solution is provided for promoting popularization and application of the LC-MS / MS technology in clinical practice.

The invention discloses a preparation method and application of a gynostemma pentaphylla exosome. According to the method, a high-activity callus is obtained by optimizing a callus induction culture medium, and a suspension culture system is established to realize biomass accumulation with the dry cell weight of 19.8 + / -0.7 g / L. A sequential progressive composite induction strategy (methyl jasmonate is added after chitosan is pre-induced for 24 hours) is innovatively adopted, so that the saponin content is remarkably increased to 102.6 + / -4.8 mg / g DW. Exosomes (P-Exos) are extracted from induced cells through an enzymolysis-magnetic bead affinity purification technology (WGA modified magnetic bead affinity capture after mild enzymolysis of a cracking buffer solution), the yield reaches 1.45 + / -0.08 mg / g cells, and the extraction efficiency is remarkably improved compared with that of a traditional gradient centrifugation method (C-Exos). Moreover, the gynostemma pentaphyllum exosome prepared by the invention can effectively improve the synthesis of skin collagen, has a better effect, can be applied to medicines for promoting skin wound repair or can be added into anti-aging cosmetics as a functional component, and has a very high application value.

The utility model discloses a magnetic porous reaction plate, relates to a biological medicine research and development device, and is used for life science experiment research using magnetic beads. The reaction plate comprises a perforated plate, a base, a cover plate and a magnet, and the magnet can be embedded between the perforated plate and the base and is matched with the bottom of the perforated plate in shape. According to the utility model, the porous reaction plate can be moved, centrifuged, shaken and the like under the condition of keeping magnetic force, so that more flexible and diversified experiment designs and experiment schemes are realized.

The invention provides a lysosome extraction method. The lysosome extraction method comprises the following steps: (1) extracting cells; (2) tissue extraction; (3) crude separation of lysosome; and (4) separating the lysosome by magnetic beads. Through EDTA-free digestion optimization, lysis solution and buffer solution formula optimization, a filter membrane interception-magnetic bead purification combined technology, a double-antibody synergistic capture strategy and a competitive mild elution system, the four core problems of low purity, large activity damage, tedious operation and strong equipment dependence in a traditional lysosome separation technology are systematically solved. Experimental data show that the lysosome yield and functional integrity of the method are obviously superior to those of a density gradient centrifugation method, and the method is suitable for trace samples and conventional equipment and has high conversion value. In the future, the technical barrier can be further optimized through automation equipment integration and multi-dimensional function verification, and the application of the method in basic research and clinical diagnosis is promoted.

The invention provides a magnetic bead for IgG antibody biotin labeling and purification and an application method, and the magnetic bead is prepared by the following steps: 1) preparing rProtein A and rTurbo ID into a protein solution; (2) mixing the protein solution with the carboxyl magnetic beads to react, so that the protein is coupled to the carboxyl magnetic beads to obtain rProtein A and rTurbo ID protein coated magnetic beads; according to the magnetic bead disclosed by the invention, biotin labeling of an antibody can be realized by applying Turbo ID enzyme, only ATP and common biotin molecules need to be used, and the cost is very low; and the biotin labeling reaction is carried out on the magnetic beads, so that the purification and biotin labeling operation is simplified, and the operation is simple and convenient.

Disclosed herein is an instrument and associated methods for a fully automated bench-top NULISA platform, comprising an X-Y-Z-gantry, a microtiter plate stage, an incubator, a quantitative PCR module, a decontamination cleaner for microtiter plates, a microtiter plate sealer, a magnetic probe and comb assembly for sample mixing and magnetic bead extraction, a storage unit for reagents and supplies, and a controller.

According to the clamping box structure and the nucleic acid extraction clamping box, a clamping box main body is matched with a mounting cavity, an extraction cavity, a buffer cavity, a waste liquid collection cavity, a liquid transfer channel, an eluent input port, a cleaning liquid input port, a combination liquid input port, a lysis solution input port, an extraction pipe interface, a collection pipe interface, a first plunger interface and a second plunger interface; the full-sealing design is achieved, and external pollution is prevented; automatic operation of the clamping box in the nucleic acid extraction instrument is facilitated, complete integration of kit preloading and extraction is realized in the automatic operation process of the clamping box, and the purity and the product concentration of a nucleic acid extraction product are improved; a universal magnetic bead method and a silica gel membrane method are compatible, extracted products can be independently stored, additional liquid transfer operation is not needed, and therefore the method is suitable for various downstream molecular biology experiments; by matching with a plunger structure, air pump equipment such as a vacuum pump does not need to be adopted in the nucleic acid extraction instrument, so that the risk of cross contamination between the interior of the instrument and the interior of the clamping box structure is reduced.

The invention discloses a gamma delta T cell efficient amplification method and application, and belongs to the technical field of cell biology. The amplification method comprises the following steps: separating PBMC (peripheral blood mononuclear cells) by adopting a density gradient centrifugation method, carrying out initial culture through a polylysine coated container, carrying out three-stage dynamic stimulation, combining with an X-VIVO 15 culture medium of 5-8% autoserum, and finally carrying out anti-gamma delta TCR magnetic bead separation to obtain high-purity cells. Through collaborative optimization of stepped factor combination, staged container coating and a low-serum system, the amplification multiple of the gamma delta T cells reaches 150-180 times, the purity is larger than or equal to 90% after purification, the cytotoxicity and the survival ability are remarkably improved, the gamma delta T cells can be efficiently used for immunotherapy of tumors and infectious diseases, and stable technical support is provided for clinical transformation of the gamma delta T cells.

Disclosed herein is an instrument and associated methods for a fully automated bench-top NULISA platform, comprising an X-Y-Z-gantry, a microtiter plate stage, an incubator, a quantitative PCR module, a decontamination cleaner for microtiter plates, a microtiter plate sealer, a magnetic probe and comb assembly for sample mixing and magnetic bead extraction, a storage unit for reagents and supplies, and a controller.

The invention discloses a nucleic acid extraction method. The method comprises the following steps: carrying out impurity removal treatment on a to-be-extracted biological sample containing nucleic acid by adopting first magnetic beads; carrying out adsorption treatment of adsorbing the nucleic acid by adopting a second magnetic bead; wherein the outer surface of the magnetic microsphere body of the first magnetic bead is provided with at least one polymer with a branched chain, and the branched chain contains a functional group. The first magnetic beads adsorb impurities after the bacterial liquid is cracked, so that a biological sample can meet the requirement of subsequent nucleic acid extraction without centrifugation, a manner of needing a centrifugation step in a traditional process is replaced, the method is more efficient and quicker, and the extracted nucleic acid is high in purity and good in quality. The invention further develops a nucleic acid extraction kit which can be suitable for operation of an automatic nucleic acid extraction instrument, and the requirements of automation and high throughput are met.

The invention discloses a multiple nucleic acid detection device and a use method, the multiple nucleic acid detection device comprises a card box and multiple chips which are communicated with each other, multiple detection of the whole process is realized in a form of integrating the extraction card box and the multiple detection chips, the sample transfer step is reduced, the pollution risk is reduced, the detection sensitivity is improved, and the detectable target range is widened. A plurality of independent functional chambers are arranged in the card box, under the cooperation of a near-end flow channel opening and a far-end flow channel opening, a reaction chamber is communicated with other functional chambers and sample injection holes required by nucleic acid reaction, and tedious magnetic bead method nucleic acid extraction, nucleic acid release and removal of impurities in a sample are realized in the card box to obtain pure nucleic acid. Meanwhile, the nucleic acid concentration is improved, the subsequent detection sensitivity can be improved, the internal part of the extraction card box, the internal part of the detection chip and the connection between the card box and the chip are fully sealed, and cross contamination among multiple reaction chambers and aerosol pollution to the environment during amplification are prevented.

The application belongs to the technical field of gene detection, and particularly relates to a biological sample nucleic acid rapid extraction method and kit based on a magnetic bead method. The method comprises the following steps in sequence: S1, liquefying a biological sample by using a liquefying solution; the liquefying treatment is to add 1-4 times the volume of the liquefying solution to the biological sample, shake and mix, and then place at room temperature for 15-30 minutes; the liquefying solution comprises guanidine salt, potassium salt and / or sodium salt, Tris-HCl, EDTA and isopropyl alcohol; S2, lysing the biological sample; adding a lysing solution and magnetic beads to the system obtained in S1, and heating at 90-100 DEG C for 3-20 minutes to lyse the biological sample; S3, cleaning the biological sample; and S4, eluting the nucleic acid. The method does not need centrifugal separation in the operation process, and can realize automatic and efficient extraction of the nucleic acid.

A lysis capsule for performing a cell lysis procedure includes a hollow body having an open first end and an open second end, a first porous membrane covering the open first end, and a second porous membrane covering the open second end, and the hollow body defines a lysis chamber between the first and second porous membranes. A plurality of non-magnetic beads and at least one magnetic element are disposed within the lysis chamber, and the pores of the first and second porous membranes are sized to retain the non-magnetic beads and the magnetic element within the lysis chamber. An internal control may be contained within the lysis chamber to validate an assay result and / or to validate the effectiveness of the cell lysis procedure. The lysis capsule may be disposed within a sample chamber of a fluidic cartridge.

The embodiment of the present application provides a nucleic acid processing method, and relates to the technical field of biomolecule detection. The nucleic acid processing method comprises the following steps: in the extraction process of the nucleic acid, the magnetic beads can be stirred, the magnetic beads can be scattered in liquid vortex, the magnetic beads can be suspended under the action of a magnetic field, and the magnetic beads cannot fall into other channels after moving away from the magnetic field, so that the loss of the magnetic beads is reduced, the nucleic acid extraction can be completed, the accuracy of inspection is improved, high-precision nucleic acid extraction is realized, and high-precision nucleic acid amplification can also be realized.

The invention discloses a construction method and application of a protein fingerprint spectrum of Tilletia foetida teliospore. The method comprises the following steps: firstly, preparing immunomagnetic beads by using a monoclonal antibody for resisting Tilletia foetida teliospore, wherein the immunomagnetic beads are used for specifically enriching target teliospore in a wheat sample; then, the captured magnetic bead-teliospore compound is placed in a formic acid-acetonitrile solution and subjected to efficient cracking through steel ball grinding, and internal protein of the teliospore is released. The released protein is subjected to MALDI-TOF-MS analysis, so that a protein fingerprint spectrum of the protein is obtained, and a characteristic protein fingerprint spectrum database of the fungal spores is constructed. The rapid identification of the Tilletia foetida teliospore is realized by comparing and analyzing the mass spectrum of the sample to be detected and the self-established database. The detection time is shortened to be within 1 h, the sensitivity reaches up to 105 spores per gram of samples, and the method has the advantages of being simple, convenient, accurate and efficient and is particularly suitable for on-site rapid screening in the field of food quality safety.

The invention discloses a 96-hole preassembled plate fat-soluble vitamin pretreatment kit, and belongs to the technical field of in-vitro diagnostic reagents. Comprising magnetic bead suspension liquid, equilibrium liquid, leacheate 1, leacheate 2 and eluent which are carried on a preassembled plate, and the magnetic bead suspension liquid comprises absolute ethyl alcohol and magnetic beads; the preparation process of the magnetic beads comprises the following steps: activating carboxyl of the carboxyl magnetic beads through EDC and NHS; then nitrochlorobenzene and triethylamine are added for a coupling reaction; mixing the coupling reaction product with pyrrolidone, ammonium persulfate and a pore-foaming agent to obtain target magnetic beads; the magnetic beads can be specifically combined with fat-soluble vitamins in human serum or plasma, efficient separation and release of an object to be detected are achieved through a magnetic separation technology, and the magnetic beads are suitable for follow-up mass spectrum quantitative analysis. The kit disclosed by the invention has the characteristics of simplicity and convenience in operation, high extraction efficiency and good stability, the pretreatment time can be remarkably shortened, the detection accuracy is improved, and an efficient solution is provided for clinical detection of fat-soluble vitamins.

The invention discloses a bubble self-stratification digital detection method and system for miRNA quantification of extracellular vesicles, and belongs to the technical field of biomedical engineering and molecular diagnosis. The technical problem to be solved is to provide a simple and sensitive tumor extracellular vesicle miRNA detection method capable of simultaneously detecting various miRNAs. According to the scheme, a to-be-detected sample and immunocapture bubbles coupled with an anti-EpCAM antibody are incubated to enrich tumor extracellular vesicles, a to-be-detected miRNA sample is obtained through in-situ ultrasonic lysis, then the to-be-detected miRNA sample, a fluorescence / DNA double-coding magnetic bead conjugate and the like are used for constructing an enzyme digestion system for enzyme digestion, multifunctional click glass microbubbles are added to capture unreacted magnetic beads, standing is conducted, self-stratification is conducted, bottom magnetic beads are collected, and the EpCAM antibody is obtained. After fluorescence imaging, an image is input into the AI automatic fluorescence counting and decoding module for counting to obtain a result. The method is used for detecting miR-21 and miR-155 in tumor extracellular vesicles, and is suitable for noninvasive early diagnosis of tumors.