1826 results about "Magnetic bead" patented technology

The invention discloses a preparation method and application of a universal renewable immunoaffinity magnetic bead, and belongs to the technical field of chemical detection.The preparation method comprises the steps that firstly, an engineered bacteriophage H6G5-M13 is constructed, then a polypeptide module is coupled to the engineered bacteriophage H6G5-M13, the polypeptide module comprises a part A, a part B and a part C from the N end to the C end, the part A is a target object recognition sequence located at the N end, and the part C is a target object recognition sequence located at the C end; the part B is a selectively added connecting sequence, the part C is an LPXTG substrate sequence and is located at the C end, and X refers to any amino acid; finally, the engineered bacteriophage is directionally coupled to the Ni < 2 + >-IDA magnetic bead through the action of Ni-6His, and then the universal and novel immunoaffinity magnetic bead is formed. The universal and replaceable immunoaffinity magnetic bead prepared by the method can be used for preparing a chemical detection reagent, and can also be used for capturing, releasing and sensing detection of cells and extracellular vesicles.

The invention relates to the technical field of nano magnetic bead preparation, and discloses a magnetic bead continuous separation device. The magnetic bead continuous separation device comprises a magnetic bead separator, a magnetic field generating device and a driving device, the driving device is used for driving the magnetic field generating device to be close to or away from the side wall of the magnetic bead separator, the magnetic bead separator is hollow, one end of the magnetic bead separator is provided with a first liquid inlet and a second liquid inlet, and the other end of the magnetic bead separator is provided with a first liquid outlet and a second liquid outlet. According to the invention, a plurality of separation processes can be integrated in the same structure, so that the system configuration is simplified, and the requirements of automation and large-scale continuous treatment in the magnetic bead purification process are effectively met.

The present invention relates to a microfluidic multiplexed immunoassay system based on flow magnet-activated cell sorting, and a method thereof. The system comprises a multiplexed immunoassay magnetic bead preparation module, a flow magnet-activated cell sorting microfluidic chip system, an optical detection module, and an image processing module, and achieves ultra-sensitive and rapid multiplexed protein quantitative detection by means of magnetic bead coding based on different magnetic contents and microfluidic chip decoding based on flow magnet-activated cell sorting. The microfluidic multiplexed immunoassay system of the present invention has the advantages of high detection sensitivity, short detection time, high signal-to-noise ratio, convenient and simple operation and low cost, is particularly suitable for multiplexed ultra-sensitive detection for monomolecular proteins, and has a potential economic value.

The invention relates to the technical field of high-throughput sequencing, and discloses a kit and a library construction method and a target variation detection and analysis method thereof, the kit comprises a pre-library construction reagent and a target region liquid phase hybridization capture reagent; wherein the pre-library construction reagent comprises a repair reagent, a joint, a connection reagent, an amplification reagent and purified magnetic beads; the target area liquid phase hybridization capture reagent comprises a hybridization reagent, a cleaning reagent, an amplification reagent, a purification magnetic bead, a target gene probe and a CNV / LOH probe. The invention provides a rapid, accurate and low-cost construction method of a library containing a targeting gene probe and a CNV / LOH probe and application of the library, through one-time library construction and sequencing, the advantages of a high-throughput targeting sequencing technology and a CNV-Seq technology are integrated; multi-gene point mutation, insertion and deletion, gene copy number variation, large-fragment copy number variation and heterozygosity deletion can be detected at the same time.

The invention discloses a preparation method of sandwich type immunomagnetic beads with high thermal stability, which comprises the following steps: by taking polystyrene microspheres as a template, introducing oil-phase Fe3O4 nanoparticles into the polystyrene microspheres through a swelling method, and then carrying out hydrolytic condensation reaction on the surface of a microsphere assembly by utilizing n-octyltrimethoxysilane OTMS to obtain the sandwich type immunomagnetic beads with high thermal stability. The preparation method comprises the following steps: carrying out surface amination modification on a silicon dioxide thin layer which is grown by hydrolysis of tetraethoxysilane TEOS to realize coating of an outermost organic shell layer, carrying out surface amination modification, and carrying out cross-linking on 1, 4-butanediol diglycidyl ether and erythritol on the surface to form an epoxy polymer layer so as to obtain the sandwich-type immunomagnetic bead with a cross-linked epoxy polymer layer introduced to the outer layer. According to the epoxy group modification process on the surface of the magnetic bead, a direct cross-linking modification process is adopted, so that the operation steps are simplified, and the obtained epoxy group magnetic bead is ensured to show excellent biocompatibility. In addition, the method omits the tedious process of functional group activation, and allows direct coupling with the antibody, thereby greatly improving the efficiency and convenience of the experiment.

The multifunctional clamping jaw is installed at the tail end of the mechanical arm through a rotary connecting plate and comprises a clamping jaw body, a first clamping jaw body and a second clamping jaw body, and the clamping jaw body is fixedly connected with the rotary connecting plate and can rotate in the Z-axis direction; the first clamping jaw is arranged on the clamping jaw body and is used for clamping a kit; the second clamping jaw is arranged on the clamping jaw body and used for clamping a magnetic bar sleeve. The utility model provides the multifunctional clamping jaw which is designed according to the magnetic bead purification requirement and can be used for compatibly clamping a kit and a magnetic bar sleeve material, the automatic carrying of the magnetic bead purification material can be realized by mounting the multifunctional clamping jaw at the tail end of a mechanical arm, the automatic carrying and placing of the kit and the magnetic bar sleeve required by the magnetic bead purification can be realized by the multifunctional clamping jaw, and the synchronous carrying can be realized; and meanwhile, materials of multiple specifications are compatible with the same set of robot, and the cost is greatly saved.

The invention relates to a method for improving chemiluminescence immunoassay sensitivity of magnetic particles, a detection kit and application thereof, firstly, magnetic beads are coupled with an antibody, then magnetic beads are sealed to obtain a magnetic bead coated antibody, sealing comprises pre-sealing and second-step sealing, glycine and ethanolamine are added in the pre-sealing process, and in the second-step sealing process, glycine and ethanolamine are added in the second-step sealing process. Casein hydrolysate and dodecyl dimethyl amine ethyl lactone are added; then coupling the antibody with alkaline phosphatase or streptavidin to obtain an alkaline phosphatase labeled antibody or a streptavidin labeled antibody; the magnetic bead coated antibody, the alkaline phosphatase labeled antibody or the streptavidin labeled antibody is used for sample detection. The method is high in sensitivity and wide in detection range, is adaptive to a common immunoassay instrument, does not need expensive equipment, and is also simple to operate; in addition, the AMH concentration with extremely low concentration can be accurately detected.

The invention provides a therapeutic drug monitoring kit and method and system, a magnetic bead extraction method is combined with LC-MS / MS detection to be applied to human blood drug concentration monitoring, a mixed magnetic bead solution containing phospholipid removal magnetic beads and to-be-detected drug adsorption magnetic beads is utilized to perform adsorption and extraction pretreatment on a to-be-detected drug in a sample, automatic treatment equipment is matched, and the concentration of the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug is detected. The pretreatment efficiency is high, the phospholipid content in the supernate is low, and the matrix effect and the chromatographic column washing pressure are greatly reduced. According to the therapeutic drug monitoring method provided by the invention, a unified pretreatment process is used, simultaneous extraction of multiple drugs is realized, and through a magnetic bead automatic extraction method, the pretreatment step of a sample can be rapidly completed, a therapeutic drug monitoring result can be rapidly obtained, the overall detection time is shortened, and the detection efficiency is improved. Therefore, a reliable laboratory examination basis is provided for individualized treatment.

The invention discloses an execution sleeve automatic separation type nucleic acid extraction and purification instrument. The instrument comprises a stirring adsorption magnetic bead execution device, a mounting frame, a linear driving device and a kit, a magnetic bead stirring and adsorbing executing device is mounted on one side of the mounting frame, a linear driving device is mounted below the magnetic bead stirring and adsorbing executing device, a kit is detachably mounted at the output end of the linear driving device, and the linear driving device is used for driving the kit to be aligned with the executing end of the magnetic bead stirring and adsorbing executing device. According to the execution sleeve automatic separation type nucleic acid extraction and purification instrument, the problem that the whole nucleic acid extraction instrument is large in size due to the fact that a loading disc needs to be rotated to switch different kits to be aligned with a magnetic rod in the prior art is solved.

The invention belongs to the technical field of nano magnetic beads, and relates to a carboxyl nano magnetic bead and application thereof in DNA capture and DNA release. The invention discloses a preparation method of carboxyl nano magnetic beads, which comprises the following steps: dissolving iminodiacetic acid in a sodium hydroxide solution, stirring in an ice bath, dropwise adding a silane coupling agent, carrying out a first heating reaction, cooling in the ice bath, dropwise adding the silane coupling agent again, carrying out a second heating reaction, and cooling in the ice bath to obtain a carboxyl silane coupling agent; and dispersing the ferroferric oxide / silicon dioxide hydroxyl magnetic compound and a carboxyl silane coupling agent in an organic solvent, and stirring for reaction to obtain the carboxyl nano magnetic beads. The carboxyl nano magnetic bead is a nano-scale magnetic bead, is large in specific surface area, good in suspension property and not easy to settle, and has multiple sites for DNA binding; the method can be used for effectively extracting DNA ladders and cfDNA in nucleic acid capture and nucleic acid release.

The invention discloses a fruit and vegetable exosome extraction method based on ligand fishing. The fruit and vegetable exosome extraction method comprises the following steps: suspending amino-functionalized Fe3O4 (at) SiO2 nanoparticles in a glutaraldehyde solution, and adding activated agglutinin to obtain Fe3O4 (at) SiO2-agglutinin nanoparticles; adding a glutaraldehyde solution into agarose gel to obtain a size exclusion chromatographic column filler; the method comprises the following steps: dispersing Fe3O4 (at) SiO2-lectin nanoparticles in a PBS buffer solution to obtain a uniform suspension, adding the uniform suspension into a size exclusion chromatographic column filler to obtain a uniform slurry, filling the size exclusion chromatographic column with the uniform slurry, and flushing with the buffer solution to form a magnetic bead stationary phase; and loading fruit and vegetable supernatant into a size exclusion chromatographic column, passing through the magnetic bead stationary phase, cleaning residual impurities combined on the exosome-magnetic bead compound by using a buffer solution, and dissociating the exosome from the magnetic beads by using a competitive eluent of the exosome to obtain the high-purity fruit and vegetable exosome. The problem that an existing exosome extraction method is difficult to meet the requirements of high efficiency, simplicity, high purity and high activity at the same time is solved.

The invention relates to an HBV magnetic bead as well as a preparation method and application thereof. The HBV magnetic beads are HBV magnetic beads with molecular tag primer sequences, poly (dT) and N HBV specific capture probes are coupled and modified, and N is a positive integer and is greater than or equal to 1. The HBV specific capture probe is selected from the following groups: (a) a pgRNA probe; (b) an S probe; (c) an X probe; (d) an rcDNA probe; (e) a cccDNA probe; wherein the pgRNA probe targets a positive chain pgRNA region of the HBV, the S probe targets a positive chain S region of the HBV, the X probe targets a positive chain X region of the HBV, the rcDNA probe targets a negative chain rcDNA region of the HBV, and the cccDNA probe targets a negative chain cccDNA region of the HBV. By adopting the novel HBV magnetic bead, the enrichment of HBV sequences can be greatly improved.

The utility model provides a magnetic frame, and relates to the technical field of biology and medical treatment. The magnetic frame comprises a magnetic frame body and a magnetic fixing piece, a placing space is arranged on one side of the magnetic frame body, and one end, away from the magnetic frame body, of the placing space is open; the two ends of the magnetic fixing piece are arranged on the left side and the right side of the opening of the containing space respectively. According to the magnetic frame, an existing magnetic frame structure is thoroughly changed, and one side of the magnetic frame body is provided with the containing space used for containing the magnetic bead incubation structure and the magnetic fixing belt used for fixing the magnetic bead incubation structure in the containing space, so that the magnetic bead incubation structure of a non-centrifugal tube is fixed.

The invention provides a circulating tumor cell screening and separating device and a circulating tumor cell detection method, and belongs to the technical field of medical detection.The circulating tumor cell screening and separating device comprises a chamber module, the chamber module is provided with a mixing chamber, a screening chamber and a waste liquid chamber, and the mixing chamber, the screening chamber and the waste liquid chamber are sequentially communicated; the control module is mounted on the cavity module, and the control module is arranged to be capable of controlling whether the liquid is discharged from the mixing cavity to the screening cavity or not according to the motion state of the control module; the temperature control module is installed in the cavity module, and the temperature control module is arranged to be capable of controlling the temperature in the mixing cavity; the magnetic enrichment module is arranged to be capable of forming a magnetic field in the mixing cavity; the CTCs screening and separating device has the advantages that the CTCs screening and separating device based on the immunomagnetic bead separation technology is provided, the device is compact in structure, small in size and convenient to operate, the function of simulating the human body constant-temperature environment to assist in reaction cultivation is achieved, and the reliability and the detection precision during detection are ensured.

The invention relates to the technical field of biological nano materials, in particular to a superparamagnetic strong anion exchange magnetic bead for virus enrichment and a preparation method and application thereof. The invention discloses a superparamagnetic strong anion exchange magnetic bead for virus enrichment and a preparation method of the superparamagnetic strong anion exchange magnetic bead. According to the superparamagnetic strong anion exchange magnetic bead, polyglycidyl methacrylate magnetic microspheres serve as raw materials, firstly, a large number of amino groups are introduced through reaction of active epoxy groups and a multi-amino compound, and then abundant quaternary ammonium salt groups are introduced through reaction of amino groups and epoxy quaternary ammonium salt under the alkaline condition. Through two times of epoxy ring-opening reactions, while enough quaternary ammonium salt groups are introduced, a large number of hydrophilic hydroxyl groups are introduced to the surfaces of the magnetic microspheres, so that the hydrophilicity of the anion exchange magnetic beads is improved, and the anion exchange magnetic beads are high in enrichment efficiency and elution efficiency and good in application prospect when being applied to enrichment of viruses.

The invention relates to the field of biological detection, in particular to a pretreatment kit for a positive sample containing chemical magnetic beads and application of the pretreatment kit. According to the method, the chemical magnetic beads are creatively applied to mass spectrum identification, and the strong adsorption capacity of the chemical magnetic beads to bacteria is utilized, so that the cost can be saved, and the pretreatment time of mass spectrum detection is greatly shortened. Meanwhile, an incubation solution is used for replacing a sample, so that the environment of bacteria is simpler, efficient adsorption of the magnetic beads to the bacteria is more facilitated, a bacteria-chemical magnetic bead compound can be extracted through simple magnetic adsorption, then the bacteria on the bacteria-chemical magnetic bead compound are eluted and detected, the purity of detected mycoprotein is very high, species identification can be achieved through mass spectrum, and the method is suitable for large-scale popularization and application. A new thought is provided for mass spectrum microorganism detection.

The invention relates to the technical field of plasmid extraction, in particular to an automatic extraction method of endotoxin-free large-volume plasmid DNA and a kit. According to the kit provided by the invention, a magnetic bead adsorption technology and a buffer system with a unique separation effect are adopted, and specially modified protein is combined with magnetic beads, so that endotoxin and protein impurities can be adsorbed; an endotoxin removing solution is adopted, so that endotoxin can be efficiently removed; the method comprises the following steps: firstly, preparing plasmid DNA, then, uniquely embedded nucleic acid adsorption magnetic beads have very strong affinity to the plasmid DNA under a certain condition and are not easy to adsorb endotoxin, and when the condition is changed, the nucleic acid adsorption magnetic beads release the adsorbed plasmid DNA, so that the purpose of rapidly separating and purifying the plasmid DNA is achieved. The plasmid DNA extracted by utilizing the kit and the extraction method disclosed by the invention is high in yield, good in purity and extremely low in endotoxin content, and can be suitable for transfecting various cells and various conventional operations including enzyme digestion, PCR (Polymerase Chain Reaction), sequencing, connection and other various downstream experiment requirements.

The invention provides a row magnetic frame. The row magnetic frame comprises an upper plate frame, a base located below the upper plate frame and a magnet holder connected with the upper plate frame and the base. The upper plate frame is provided with two rows of mounting holes which are distributed in an array and are used for placing test tubes, and each row of mounting holes are arranged along the length direction of the magnet retainer; a plurality of magnet mounting grooves which are arrayed in the length direction are formed in the magnet holder, and a magnet assembly is arranged in each magnet mounting groove; each magnet assembly comprises a first magnet and a second magnet which are symmetrically arranged front and back; the third magnet and the fourth magnet are respectively positioned on the left and right sides of the first magnet and the second magnet; the magnetic pole directions of the first magnet and the second magnet are opposite and are perpendicular to the magnetic pole directions of the third magnet and the fourth magnet. Therefore, by improving the magnetic circuit design, the magnetic field intensity of the magnetic frame is improved, so that the magnetic bead adsorption capability is enhanced.

The invention relates to the field of precision casting, and particularly discloses a gas turbine blade centre hole depth measuring method, which comprises the following steps: analyzing a centre hole section structure and a magnetic bead contact state size through UG drawing software, performing sectioning analysis on a gas turbine blade centre hole axis surface, performing tangent constraint on a magnetic bead contour and a centre hole inner contour, and determining the depth of the centre hole; measuring the distance from the center of the magnetic bead to the inner end of the centre hole; putting the magnetic bead into the centre hole of the blade, measuring the center position of the magnetic bead by adopting a three-coordinate measuring instrument, and calculating the actual centre hole depth of the blade by combining the dimension guarantee of the three-coordinate measuring instrument on the profile tolerance of the inner wall of the centre hole; according to the method, by means of the tangent constraint of the magnetic bead and the centre hole in the blade tenon, the hub degree of the magnetic bead is measured through the three-coordinate measuring instrument, the depth of the centre hole is calculated, the depth of the centre hole can be accurate to 0.01 mm, the measurement precision is high, and the precision of follow-up positioning and clamping machining of the gas turbine blade through the centre hole is guaranteed.

The invention discloses pancreatic lipase immobilized composite nano magnetic beads, a preparation method and application, ferroferric oxide is adopted as a magnetic core, dopamine-mediated growth is performed to form a core-shell structure, then the periphery of the core-shell structure is wrapped with a metal organic framework to form the composite nano magnetic beads, and pancreatic lipase is immobilized by using the composite nano magnetic beads as an adsorption carrier. The enzyme loading amount, the repeated utilization rate and the storage stability of the ferroferric oxide nano-particles are remarkably improved, the enzyme immobilization efficiency and the enzyme activity are improved, and meanwhile, the prepared pancreatic lipase immobilized composite nano-magnetic beads have excellent stability and biocompatibility, are green and pollution-free and can be repeatedly used; the composite nano magnetic beads are synthesized by adopting a solvothermal method, the product is easy to separate, the synthesis process is simple, the enzyme immobilization efficiency can be regulated and controlled by regulating and controlling the synthesis process, the structure of an active ligand can be screened and analyzed from natural products with numerous and jumbled components, and the active ligand has a certain lipid-lowering potential and can be used for preparing the nano magnetic beads. The compound can be used as a potential lead compound of lipid-lowering drugs.

The invention relates to the technical field of detection and analysis, and discloses a nanoparticle for detecting the concentration of methotrexate as well as a preparation method and application of the nanoparticle. The nanoparticles disclosed by the invention comprise hairpin DNA which is connected with the gold nanoparticles and is provided with a marking group and aptamer chain DNA which can be specifically recognized by methotrexate, and the aptamer chain DNA and walking chain DNA are hybridized into double-chain DNA which is connected to a magnetic bead, on one hand, the concentration of MTX is measured by marking a DNA chain of a fluorophore and collecting a fluorescence signal; on the other hand, after sodium chloride is added into the gold nanoparticles without DNA protection, the color of the gold nanoparticles changes under a high-salt condition, and the concentration of MTX is measured by collecting ultraviolet values. According to the nanoparticles provided by the invention, all parts interact with one another, signals are amplified, the effect of double detection of MTX concentration is exerted, and a powerful strategy is provided for accurately mastering the drug concentration in real time and improving the treatment safety and effect.

The invention relates to the field of biological detection, in particular to a positive sample pretreatment kit and application thereof. The method comprises the following steps: providing a simple survival environment for bacteria by utilizing incubation liquid, and then utilizing the interaction of opposite electric properties between chemical magnetic beads and the bacteria, that is, when the bacteria in a positive sample are negatively charged, the chemical magnetic beads are positive magnetic beads; or when the bacteria in the positive sample are positively charged and the chemical magnetic beads are negative electromagnetic beads, the bacteria-magnetic bead compound is obtained, the operation is simple, the cost is low, the enrichment efficiency is high, the impurity interference resistance is high, the activity state of the enriched bacteria is not changed, the method can be used for subsequent mass spectrum identification and metabolic activity analysis, the application range is wide, and the method is suitable for popularization and application. Various in-vitro samples can be universally enriched, and the method has great significance on the development of the IVD field.

The utility model belongs to the field of microorganism extraction equipment, and particularly relates to a microorganism extraction system. Comprising a microorganism extraction container, the microorganism extraction container contains a to-be-extracted solution and magnetic beads for adsorbing microorganisms, and the to-be-extracted solution contains the microorganisms to be extracted; the workbench is provided with a container placing part for placing the microorganism extraction container; the magnetic bead adsorption mechanism comprises a magnetic attraction assembly and a magnetic attraction state switching assembly used for changing the magnetic attraction state, and the magnetic attraction assembly has magnetism and is used for adsorbing magnetic beads located in the microorganism extraction container; and the controller is electrically connected with the magnetic attraction state switching assembly and is used for changing the state of the magnetic attraction assembly or changing the relative state of the magnetic attraction assembly and the microorganism extraction container. The operation is simple and convenient, the automatic operation of microorganism extraction is favorably realized, the difficulty of microorganism extraction is reduced, and the efficiency of microorganism extraction is improved.

The invention relates to the technical field of nano magnetic bead preparation, and discloses a magnetic bead continuous separation system and a control method. The system comprises a magnetic bead separation device which is of a hollow pipe structure, the pipe wall of the magnetic bead separation device can be magnetized and demagnetized, one end of the magnetic bead separation device is provided with a first liquid inlet and a second liquid inlet, and the other end of the magnetic bead separation device is provided with a first liquid outlet and a second liquid outlet; the stock solution tank is connected to the first liquid inlet through a first flow channel; the waste liquid tank is connected to the first liquid outlet through a fifth flow channel, and a first magnetic bead sensor is arranged on the fifth flow channel; a liquid inlet of the product tank is connected to the second liquid outlet through a sixth flow channel, and a second magnetic bead sensor is arranged on the sixth flow channel; a liquid inlet of the stirring kettle is connected to the second liquid outlet through a seventh flow channel, and a liquid outlet of the stirring kettle is connected to the first liquid inlet through a second flow channel; a liquid outlet of the washing liquid tank is connected to the first liquid inlet through a third flow channel and is connected to the second liquid inlet through a fourth flow channel. The system has the advantage of meeting the requirements of continuous, automatic and infinite batch separation and purification of the magnetic beads.

The invention relates to the technical field of new material application, and discloses a magnetic bead material for enriching a sulfydryl peptide fragment and a preparation method thereof.The magnetic bead material prepared from micron carboxyl magnetic beads, dodecane dicarboxylic acid dihydrazide and SPSP has a long chemical reaction connecting arm, so that a reaction group has the better freedom degree, the reaction with sulfydryl is better facilitated, and the magnetic bead material can be used for enriching the sulfydryl peptide fragment. The enrichment efficiency is relatively high. When the magnetic bead material is prepared, micron carboxyl magnetic beads and dodecane dicarboxylic acid dihydrazide are based on hydrazine group and carboxyl condensation, the condensation reaction is more selective and more stable, the reaction is carried out under a milder condition, the reaction product is stable, and the reaction efficiency is higher at a lower reaction temperature. Dodecane dicarboxylic acid dihydrazide and SPSP are based on a reaction of N-succinimide and hydrazine, N-succinimide has high specificity to amino-containing molecules, side reactions are reduced, the reaction is generally carried out at room temperature, strong acid and alkali conditions are not needed, and protection of other sensitive groups is facilitated.

The invention discloses a method for screening a non-natural nucleic acid aptamer of targeted thrombin based on streptavidin magnetic beads and application, and belongs to the technical field of biology. According to the method, an initial screening library is efficiently constructed through a primer extension technology, and a chemical modification SELEX technical process is simplified; a streptavidin magnetic bead separation technology is introduced, so that the screening specificity and efficiency are improved, the screening period is remarkably shortened, and the cost is reduced; by selecting non-natural nucleic acid modification sites and adopting a strategy of combining reverse screening and forward screening, high specificity and affinity of the aptamer are ensured, and limitation of an existing screening method is overcome; the screened non-natural nucleic acid aptamer shows high affinity and excellent stability, the aptamer with a specific structure and function is successfully obtained by optimizing the proportion of nucleoside triphosphate monomers and extension reaction conditions, and a wide application prospect is provided for the fields of drug development, biosensor preparation and the like. A new path is opened up for research and application of the nucleic acid aptamer.

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The utility model belongs to the technical field of cables, and particularly relates to a shielded cable, which comprises a conductor, the conductor is formed by mutually twisting a plurality of wires, an insulating layer is wrapped outside the conductor, and a filling layer is wrapped outside the insulating layer. The outer side of the filling layer is wrapped with shielding layers layer by layer. According to the utility model, the shielding net is arranged in the shielding layer, the plurality of rows of magnetic beads are arranged in the magnetic bead mounting layer in a surrounding manner, and the conductive coating is coated between the shielding layer and the magnetic bead mounting layer through the conductive coating, so that the influence of a magnetic field effect generated in a cable electrifying process on peripheral cables, equipment and instruments is effectively prevented; the use efficiency and the service life of peripheral related equipment are relatively improved, particularly for high-precision instruments, the accuracy of the cable is further guaranteed, and the situation that the cable is vivid by external electromagnetic waves is also avoided; and the anti-interference capability of the cable can be improved.

The invention discloses a magnetic bead continuous separation process and device, and the process comprises the following steps: firstly applying a magnetic field to a magnetic bead separator, then continuously adding a stock solution into the magnetic bead separator, forming a magnetic bead capturing area and a magnetic bead separation area under the action of the magnetic field, capturing magnetic beads to the capturing area, discharging impurities to a waste liquid tank from a liquid outlet, and separating the impurities from the waste liquid tank. The concentration of the magnetic beads at the liquid outlet exceeds a set value; after the magnetic field is relieved, washing liquid is continuously added into the magnetic bead separator, and the magnetic beads are flushed out and collected into an intermediate tank; applying the magnetic field again, and continuously adding the collected liquid into the magnetic bead separator for separation again; after the magnetic field is relieved, washing liquid is injected into an inlet of the magnetic bead capturing area, so that the magnetic beads are flushed out until the concentration of the magnetic beads at a liquid outlet is lower than a set value; and circulating the steps until the treatment is finished. The process is automatically operated through a PLC (Programmable Logic Controller) control system. The magnetic bead separation device has the advantages that automatic feeding and discharging are achieved, multiple operation steps are completed in the same device, and multi-round repeated separation and automatic completion of magnetic beads are achieved.

The embodiment of the invention discloses a bipolar electrochemical luminescence micro-fluidic chip and application thereof. The bipolar electrochemical luminescence micro-fluidic chip comprises an ITO (Indium Tin Oxide) electrode 1, a PDMS (Polydimethylsiloxane) chip main body and an ITO electrode 2 from top to bottom. According to the invention, horseradish peroxidase (HRP) is used as a signal label to be coupled with a labeled antibody (Ab2), magnetic beads are coupled with a coated antibody (Ab1), during detection, a coupled reagent and a sample to be detected are injected into the chip through an injection pump at the same time, the antibody and a detection substance AFP are mixed in the chip through a spiral microchannel to form an immune sandwich compound, and the immune sandwich compound is used for detecting the AFP in the chip. The electrochemical luminescence sensor is used for detecting the AFP, then is captured on the surface of the ITO electrode in the detection cavity under the action of the electromagnet, and finally outputs an electrochemical luminescence signal through electrical stimulation to realize detection, so that the AFP can be rapidly and simply detected at low cost, and the electrochemical luminescence sensor can be widely applied to the screening and diagnosis process of HCC.