621 results about "Magnetic bead" patented technology

The invention relates to an HBV magnetic bead as well as a preparation method and application thereof. The HBV magnetic beads are HBV magnetic beads with molecular tag primer sequences, poly (dT) and N HBV specific capture probes are coupled and modified, and N is a positive integer and is greater than or equal to 1. The HBV specific capture probe is selected from the following groups: (a) a pgRNA probe; (b) an S probe; (c) an X probe; (d) an rcDNA probe; (e) a cccDNA probe; wherein the pgRNA probe targets a positive chain pgRNA region of the HBV, the S probe targets a positive chain S region of the HBV, the X probe targets a positive chain X region of the HBV, the rcDNA probe targets a negative chain rcDNA region of the HBV, and the cccDNA probe targets a negative chain cccDNA region of the HBV. By adopting the novel HBV magnetic bead, the enrichment of HBV sequences can be greatly improved.

The utility model provides a magnetic frame, and relates to the technical field of biology and medical treatment. The magnetic frame comprises a magnetic frame body and a magnetic fixing piece, a placing space is arranged on one side of the magnetic frame body, and one end, away from the magnetic frame body, of the placing space is open; the two ends of the magnetic fixing piece are arranged on the left side and the right side of the opening of the containing space respectively. According to the magnetic frame, an existing magnetic frame structure is thoroughly changed, and one side of the magnetic frame body is provided with the containing space used for containing the magnetic bead incubation structure and the magnetic fixing belt used for fixing the magnetic bead incubation structure in the containing space, so that the magnetic bead incubation structure of a non-centrifugal tube is fixed.

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The invention relates to the technical field of medical detection, in particular to a method for detecting aldosterone, cortisol, deoxycorticosterone and cortisone in plasma through LC-MS / MS. The method comprises the following steps: (a) mixing a plasma sample with an internal standard solution to obtain a mixed solution; (b) adding the mixed solution into the magnetic bead suspension, sequentially carrying out adsorption, weak washing, strong washing and elution, and collecting an eluent; (c) carrying out liquid nitrogen blowing and redissolving on the eluent, and then carrying out LC-MS / MS detection; wherein the surfaces of the magnetic beads are modified with steroid adsorption groups, and the target detection objects are aldosterone, cortisol, deoxycorticosterone and cortisone. The detection method is convenient and simple, the detection efficiency is higher than that of a traditional method, and a feasible solution is provided for promoting popularization and application of the LC-MS / MS technology in clinical practice.

The invention discloses a gamma delta T cell efficient amplification method and application, and belongs to the technical field of cell biology. The amplification method comprises the following steps: separating PBMC (peripheral blood mononuclear cells) by adopting a density gradient centrifugation method, carrying out initial culture through a polylysine coated container, carrying out three-stage dynamic stimulation, combining with an X-VIVO 15 culture medium of 5-8% autoserum, and finally carrying out anti-gamma delta TCR magnetic bead separation to obtain high-purity cells. Through collaborative optimization of stepped factor combination, staged container coating and a low-serum system, the amplification multiple of the gamma delta T cells reaches 150-180 times, the purity is larger than or equal to 90% after purification, the cytotoxicity and the survival ability are remarkably improved, the gamma delta T cells can be efficiently used for immunotherapy of tumors and infectious diseases, and stable technical support is provided for clinical transformation of the gamma delta T cells.

The application belongs to the technical field of gene detection, and particularly relates to a biological sample nucleic acid rapid extraction method and kit based on a magnetic bead method. The method comprises the following steps in sequence: S1, liquefying a biological sample by using a liquefying solution; the liquefying treatment is to add 1-4 times the volume of the liquefying solution to the biological sample, shake and mix, and then place at room temperature for 15-30 minutes; the liquefying solution comprises guanidine salt, potassium salt and / or sodium salt, Tris-HCl, EDTA and isopropyl alcohol; S2, lysing the biological sample; adding a lysing solution and magnetic beads to the system obtained in S1, and heating at 90-100 DEG C for 3-20 minutes to lyse the biological sample; S3, cleaning the biological sample; and S4, eluting the nucleic acid. The method does not need centrifugal separation in the operation process, and can realize automatic and efficient extraction of the nucleic acid.

A lysis capsule for performing a cell lysis procedure includes a hollow body having an open first end and an open second end, a first porous membrane covering the open first end, and a second porous membrane covering the open second end, and the hollow body defines a lysis chamber between the first and second porous membranes. A plurality of non-magnetic beads and at least one magnetic element are disposed within the lysis chamber, and the pores of the first and second porous membranes are sized to retain the non-magnetic beads and the magnetic element within the lysis chamber. An internal control may be contained within the lysis chamber to validate an assay result and / or to validate the effectiveness of the cell lysis procedure. The lysis capsule may be disposed within a sample chamber of a fluidic cartridge.

The invention discloses a construction method and application of a protein fingerprint spectrum of Tilletia foetida teliospore. The method comprises the following steps: firstly, preparing immunomagnetic beads by using a monoclonal antibody for resisting Tilletia foetida teliospore, wherein the immunomagnetic beads are used for specifically enriching target teliospore in a wheat sample; then, the captured magnetic bead-teliospore compound is placed in a formic acid-acetonitrile solution and subjected to efficient cracking through steel ball grinding, and internal protein of the teliospore is released. The released protein is subjected to MALDI-TOF-MS analysis, so that a protein fingerprint spectrum of the protein is obtained, and a characteristic protein fingerprint spectrum database of the fungal spores is constructed. The rapid identification of the Tilletia foetida teliospore is realized by comparing and analyzing the mass spectrum of the sample to be detected and the self-established database. The detection time is shortened to be within 1 h, the sensitivity reaches up to 105 spores per gram of samples, and the method has the advantages of being simple, convenient, accurate and efficient and is particularly suitable for on-site rapid screening in the field of food quality safety.

The invention discloses a 96-hole preassembled plate fat-soluble vitamin pretreatment kit, and belongs to the technical field of in-vitro diagnostic reagents. Comprising magnetic bead suspension liquid, equilibrium liquid, leacheate 1, leacheate 2 and eluent which are carried on a preassembled plate, and the magnetic bead suspension liquid comprises absolute ethyl alcohol and magnetic beads; the preparation process of the magnetic beads comprises the following steps: activating carboxyl of the carboxyl magnetic beads through EDC and NHS; then nitrochlorobenzene and triethylamine are added for a coupling reaction; mixing the coupling reaction product with pyrrolidone, ammonium persulfate and a pore-foaming agent to obtain target magnetic beads; the magnetic beads can be specifically combined with fat-soluble vitamins in human serum or plasma, efficient separation and release of an object to be detected are achieved through a magnetic separation technology, and the magnetic beads are suitable for follow-up mass spectrum quantitative analysis. The kit disclosed by the invention has the characteristics of simplicity and convenience in operation, high extraction efficiency and good stability, the pretreatment time can be remarkably shortened, the detection accuracy is improved, and an efficient solution is provided for clinical detection of fat-soluble vitamins.

The invention discloses a bubble self-stratification digital detection method and system for miRNA quantification of extracellular vesicles, and belongs to the technical field of biomedical engineering and molecular diagnosis. The technical problem to be solved is to provide a simple and sensitive tumor extracellular vesicle miRNA detection method capable of simultaneously detecting various miRNAs. According to the scheme, a to-be-detected sample and immunocapture bubbles coupled with an anti-EpCAM antibody are incubated to enrich tumor extracellular vesicles, a to-be-detected miRNA sample is obtained through in-situ ultrasonic lysis, then the to-be-detected miRNA sample, a fluorescence / DNA double-coding magnetic bead conjugate and the like are used for constructing an enzyme digestion system for enzyme digestion, multifunctional click glass microbubbles are added to capture unreacted magnetic beads, standing is conducted, self-stratification is conducted, bottom magnetic beads are collected, and the EpCAM antibody is obtained. After fluorescence imaging, an image is input into the AI automatic fluorescence counting and decoding module for counting to obtain a result. The method is used for detecting miR-21 and miR-155 in tumor extracellular vesicles, and is suitable for noninvasive early diagnosis of tumors.

The invention discloses a protein quantitative detection method which comprises the following steps: respectively constructing a capture probe for capturing antibody coupling magnetic beads and oligonucleotides and a detection probe for detecting antibody coupling oligonucleotides through a biorthogonal chemical coupling method; then, target protein is captured by using a biorthogonal chemical coupling and magnetic bead sandwich method comprising the steps of capture probe combination, closing, washing, detection probe combination and the like, and accurate quantification of low-abundance protein in a complex sample is realized by combining a subsequent digital PCR (Polymerase Chain Reaction) technology.

The invention relates to the technical field of cashmere blended yarn preparation, and discloses wool mixing equipment and process with antibacterial and antistatic functions for cashmere blended yarn preparation, and the wool mixing equipment comprises a feeding bin, a feeding cylinder fixedly mounted at the upper end of the feeding bin, an opening device fixedly connected with the feeding bin, and an air mixing device fixedly connected with the opening device, according to the wool blending equipment, the motor is used for driving the rotating rod to rotate, the first telescopic rod rotates and extends out of the first sleeve through magnetic repulsive force generated by the magnetic plate on the first magnetic beads, the agglomerated fibers are filtered, and the fibers are uniformly mixed. The air cylinder pushes the second telescopic rods to move to the positions above the agglomerated fibers, the magnetic attraction force of the magnetic blocks to the upper ends of the magnetic rods enables the magnetic rods to turn over, the second telescopic rods are downwards inserted into the agglomerated fibers, then the air cylinder retracts, the fixing rods and the sliding rods retract one by one, and the agglomerated fibers are effectively dispersed through the two corresponding second telescopic rods.

The invention provides a multicolor visual semi-quantitative detection method for miRNA in skin interstitial fluid, which comprises the following steps: extracting a skin interstitial fluid sample in a minimally invasive manner by combining a microneedle patch with a vacuum negative pressure technology; the method comprises the following steps: incubating a skin interstitial fluid sample and a magnetic nanoprobe of which the surface is immobilized with a hairpin probe H1, opening H1 in the presence of target miRNA, triggering a subsequent hybridization chain reaction of a hairpin probe H2 and H3, and constructing a biotin-rich DNA compound on the surface of a magnetic bead; the method comprises the following steps: carrying out enzyme labeling by using an alkaline phosphatase-streptavidin conjugate, catalyzing ascorbic acid phosphate to generate ascorbic acid to reduce potassium iodate, and generating an iodine elementary substance to etch a gold nano bipyramid, so that the color of a solution is changed, and high-sensitivity detection on target miRNA is realized. The multi-color visual detection method provided by the invention is combined with the gold nano bipyramid localized surface plasmon resonance property change, so that near-full spectrum color change can be generated, and the portability of the sensor and the accuracy of visual analysis can be improved.

The invention discloses a method and a device suitable for CUAMP. The invention discloses a method for extracting liriodendron tulipifera cell nucleuses by a Tag technology and application of the liriodendron tulipifera cell nucleuses. Hybridized liriodendron tulipifera calluses are used as materials, and protoplasts are released through enzymolysis solution vacuumizing and mild enzymolysis for 1.5 h; the method comprises the following steps: sequentially purifying by using a W5 solution and mannitol, detecting the activity by FDA, cracking by using an NE buffer solution, and washing by using a Wash buffer solution in two steps, thereby obtaining 2 * 10 < 6 >-3 * 10 < 6 > high-purity and high-integrity cell nucleuses. The obtained core is clean in background and complete in membrane structure, and can be directly used for CUTamp; tag is used for building a library, so that the magnetic bead capturing efficiency is improved by 46%, and protein-DNA interaction high-throughput sequencing under the condition of low sample size is realized. The method disclosed by the invention is simple and convenient to operate and good in repeatability, and provides key technical support for epigenetic research of rare tree species such as liriodendron tulipifera.

The invention provides a magnetic bead extraction reagent, a kit and an extraction method. The magnetic bead extraction reagent is characterized by comprising a magnetic bead liquid I and a magnetic bead liquid II, the magnetic bead liquid I contains magnetic beads I; the magnetic bead liquid II contains magnetic beads II and does not contain an alcohol solvent; the magnetic beads I are used for removing impurities from a to-be-extracted biological sample containing nucleic acid, and the magnetic beads II are used for adsorbing the nucleic acid. By adopting the reagent or the kit disclosed by the invention, the bacterial liquid can be directly extracted without centrifugal treatment, and high-quality nucleic acid can be directly extracted after a bacterial liquid operation program is added. The flux is large, and nucleic acid of 32 samples can be extracted at most at a time. By utilizing the reagent, the kit and the matched procedure, the activity of the hydroxyl magnetic beads is ensured, and high-quality plasmids are extracted.

This invention provides a CRISPR / Cas12a fluorescence detection method for FocTR4 based on magnetic bead-ssDNA-HRP probes, belonging to the field of nucleic acid detection technology. This invention involves isothermal amplification of the FocTR4 target sequence using RPA primers; then, a magnetic bead-ssDNA-HRP probe is prepared; and the Cas12a / crRNA complex, RPA amplification product, and probe are co-incubated. The activated Cas12a cleaves the probe, releasing HRP. After magnetic separation, a colorimetric solution containing OPD is added, and the presence and concentration of FocTR4 are determined by detecting the fluorescence signal. This invention combines the high efficiency of CRISPR / Cas12a cleavage with the high sensitivity and strong specificity of RPA technology, achieving highly sensitive and specific detection of FocTR4, with a linear detection range of 10. 2 ~10 7 With a detection limit as low as 70 CFU / mL, it can quickly and accurately detect Foc TR4 in soil samples, enabling rapid on-site detection and providing reliable technical support for the early diagnosis and control of banana wilt disease.

The invention relates to a circulating tumor cell enrichment, separation and dyeing integrated system and application thereof.The circulating tumor cell enrichment, separation and dyeing integrated system is composed of a bottom plate module, a platform module, a multi-pipette module, a power module and a shell module; the platform module comprises a sample tube rotating and uniformly mixing module, a magnetic adsorption module, a reagent module, a waste liquid pool module and a gun head module, and the sample tube rotating and uniformly mixing module, the magnetic adsorption module and the reagent module are fixedly mounted on a bottom plate I in sequence. The CTC separation and dyeing integrated system is developed on the basis of the immunomagnetic bead technology, enrichment separation and immunofluorescent dyeing of CTC can be automatically completed by running a program, manual operation errors are effectively avoided through a programmed process, and the CTC separation and dyeing integrated system is particularly suitable for clinical CTC detection. The system is also suitable for other enrichment and separation applications based on an immunomagnetic bead technology, including but not limited to enrichment and separation of biological macromolecules such as cells, exosomes, proteins and the like.

The utility model discloses a magnetic separation device which comprises a separation bag and a magnetic frame. The separation bag is formed by connecting a plurality of independent separation chambers in series, each separation chamber contains a magnetic bead coupled with a single antibody, and a filter screen of which the aperture is far smaller than that of the magnetic bead is arranged between the adjacent separation chambers; through the adsorption effect of the magnets on the magnetic frame, the purpose of separating different subtype T cells in plasma / serum can be achieved without a solvent, and meanwhile, a slidable clamp is arranged on the magnetic frame to push the plasma.

This invention relates to the field of chemiluminescence immunoassay technology, specifically to a magnetic bead coating method and its application for improving the luminescence level of p-tau 217. This invention optimizes the process of coating p-tau 217 capture antibodies with carboxyl magnetic beads. By gradient adjustment of the input ratio of EDC and NHS, the optimal activator concentration is obtained to enhance the magnetic bead coupling activity, increase the antibody coupling efficiency, and thus improve the signal values ​​of antigen samples and clinical samples. This invention has broad application prospects for the development of highly sensitive detection of low-abundance proteins such as p-tau 217.

A solid-core ring-magnet having one or more cavities is provided. The magnet can have an overall cylindrical shape or a rectangular-prism shape. In either case, a portion of cavity walls of the magnet are ring shaped, causing the magnetic field lines to emanate from the magnet so that the bead formation is in the shape of a ring. A bead separation magnet having a discontinuous or segmented cavity wall is also provided. The segmented cavity wall causes bead formation to form in a segmented or gapped ring to allow for easier manual pipetting. Also provided are systems and kits having the inventive magnets. Methods of purifying a macromolecule using the inventive magnets are also provided.

The application provides a kit for extracting RNA and application thereof. In the application, deoxyribonuclease (DNase) is used for DNA digestion in the process of magnetic bead extraction. The reaction environment can meet the optimal reaction environment requirement of the deoxyribonuclease (DNase), and at the same time, a hydrophobic environment is provided when the magnetic beads are adsorbed, so as to ensure the yield of nucleic acid. In addition, an emulsion of an oil is added to increase the total volume during the extraction operation, so as to facilitate the convenience of automatic extraction operation of the instrument. The extracted RNA has high yield and high purity, and the method can easily realize automatic extraction of the instrument. The requirement of extracting high-purity RNA quickly and conveniently in the process of clinical practical application is solved, the difficulty and cost of extracting high-purity RNA are greatly reduced, and the extracted RNA can be well adapted to subsequent RT-PCR and RNA-seq application.

This utility model relates to the field of medical diagnostic analysis technology, specifically an RFID-based reagent buffer device. It includes a test tube holder, an electronic tag fixed to one side of the test tube holder, multiple RFID readers mounted on the inner wall of a temporary storage rack, an anti-precipitation component mounted on a cooling module, and a control circuit board fixed to the inner bottom of the temporary storage rack. When the electronic tag on the reagent rack is facing the RFID reader, the RFID reader can decode the electronic tag signal. The anti-precipitation component drives the reagent tubes containing magnetic beads / microspheres to rotate. The cooling module, the extraction motor, the RFID reader, and the anti-precipitation component are all electrically connected to the control circuit board. This utility model can automatically identify the reagent rack, achieving a higher degree of automation, and can also drive the reagent tubes containing magnetic beads / microspheres to rotate, ensuring that the concentration at the bottom of the reagent tube is consistent with the concentration in the middle of the reagent tube, thus guaranteeing the accuracy of the detection results after extraction by the detector's extraction head.

The invention relates to the field of gene mutation detection, and particularly discloses a POCT (point-of-care testing) detection method for V617F mutation of a human JAK2 gene. According to the method, an integrated detection tube is divided into a cell lysis-nucleic acid binding magnetic bead area, a nucleic acid cleaning area and a nucleic acid elution-amplification area from top to bottom in sequence through a plurality of hydrophobic layers, the cell lysis-nucleic acid binding magnetic bead area is pre-filled with a lysis solution, the nucleic acid cleaning area is pre-filled with a nucleic acid cleaning solution, and the nucleic acid elution-amplification area is pre-filled with a nucleic acid elution solution. A nucleic acid elution-amplification system is pre-installed in the nucleic acid elution-amplification area, in the detection process, only a sample needs to be added into the cell lysis-nucleic acid binding magnetic bead area, a corresponding instrument is inserted, and operation is conducted, so that nucleic acid extraction, amplification and result reporting can be completed, and additional manual processing steps are not needed. The method has the advantages of simplicity and convenience in operation, short time consumption, high sensitivity, strong specificity, low pollution risk and the like, can be directly used for whole blood sample detection, meets the rapid diagnosis requirements of clinical outpatient service, and is particularly suitable for auxiliary diagnosis of BCR-ABL negative myeloproliferative tumors.

The present disclosure relates to a method of manipulating magnetic beads in a liquid inside a conduit, wherein the method comprises generating a variable magnetic field using at least one magnet or electromagnet to mix the magnetic beads in the liquid; and wherein a biological or chemical species is attached to the surface of the magnetic beads.

The invention discloses a group of hydroxyl magnetic bead extraction and capture probes for GBSRNA, a kit and an extraction method, and relates to the technical field of nucleic acid extraction. The capture probe provided by the invention comprises five nucleotide sequences, the five nucleotide sequences are loaded on a hydroxyl magnetic bead and are used for preparing the kit for extracting the GBSRNA, the extraction efficiency of the RNA is greatly improved by rapidly splitting bacteria and releasing the RNA and specifically capturing the GBSRNA by utilizing the probe on the magnetic bead, and finally, DNA impurities are removed by utilizing DNase, so that the high-purity RNA is obtained. The technical problem that the extraction efficiency of RNA is relatively low due to relatively high content of impurities in an extract caused by inaccurate extraction is solved.

The utility model provides a reciprocating type driving rod with an electromagnet, which belongs to the technical field of medical detection and comprises a shaft rod and an electromagnet module, the electromagnet module is fixedly connected with the shaft rod and comprises an iron core, a coil and a protective cover, the protective cover is connected with the iron core, and an annular sealing cavity is formed between the protective cover and the iron core. The coil is located in the annular sealing cavity and wound around the iron core with the shaft rod as the center. The device has the beneficial effects that a magnetic field can be generated or disconnected by controlling the on-off of the current of the coil in the enrichment process, and the coil is wound on the iron core by taking the shaft rod as the center, so that when the shaft rod is positioned in the center of the reaction chamber, the magnetic field can be generated or disconnected on the premise of ensuring that the shaft rod and the electromagnet module do not generate position interference; and the magnetic field generated by the electromagnet module is also located at the central position of the reaction chamber, so that the magnetic fields at all positions in the reaction chamber are uniformly distributed, and the magnetic enrichment effect of the CTCs-magnetic bead compound is ensured.

The utility model provides a magnetic bead adding device which is characterized in that a pen tube is detachably connected with the lower end of a pen container, a movable cylinder is movably arranged in the pen container, the upper end of the movable cylinder extends out of the upper end of the pen container, the lower end of the movable cylinder is a closed end, the movable cylinder can move between a first position and a second position, and a pen cap is arranged at the top of the movable cylinder. A movable hole is formed in the first end of the movable cylinder, the guide piece is arranged in the pen tube and provided with a first through hole communicated with the magnetic bead guide cylinder, the first end of the magnetic bead guide rod is connected with the first end of the movable cylinder, and a containing groove is formed in the second end of the magnetic bead guide rod. Due to the fact that separation and adding of the magnetic beads can be achieved through the movable cylinder, the magnetic bead guide rod, the magnetic bead guide cylinder and the guide piece, compared with an existing magnetic bead adding device, the magnetic bead adding device is simple in structure and smaller in size, operation of workers is greatly facilitated, and the use range of the magnetic bead adding device is greatly expanded.

A fluid sample is dispensed into a sample chamber of a fluidic cartridge, the sample chamber containing non-magnetic beads, a magnetic element, and an internal control reagent contained within an internal control pellet including an internal control for validating an assay result and / or to validate the effectiveness of a cell lysis procedure. The magnetic element is exposed to a magnetic field, thereby causing movement of the magnetic element, which causes movement of the non-magnetic beads. Movement of the non-magnetic beads causes cells contained within the fluid sample to lyse and release nucleic acids. The internal control reagent dissolves in the presence of the fluid sample or movement of the non-magnetic beads disintegrates the internal control pellet, thereby releasing the internal control into the fluid sample. Movement of the magnetic element and the non-magnetic beads causes the internal control to be distributed within the fluid sample.

The invention discloses an aptamer-based oxytetracycline fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit as well as a preparation method and a detection method thereof, and relates to the technical field of analysis and detection.The detection method comprises the following steps: mixing and incubating a sample to be detected, an oxytetracycline specific aptamer, a complementary chain and a magnetic bead curing capture probe; magnetically separating out the mixed and incubated magnetic beads, curing and capturing the probe, and washing to obtain a magnetic compound; adding PCR reaction liquid containing the primer pair and a TaqMan probe into the magnetic compound, carrying out fluorescent quantitative PCR amplification, and monitoring a fluorescent signal in real time to obtain a Ct value; establishing a standard curve between the oxytetracycline concentration and the Ct value of the fluorescent quantitative PCR, and determining the oxytetracycline concentration in the sample to be detected according to the standard curve. Based on an indirect competitive mechanism, through signal conversion design, combination of oxytetracycline and the aptamer is converted into quantitative analysis of free sDNA, and through integration of three technologies of aptamer free recognition, magnetic bead targeted capture and qPCR signal amplification, the sensitivity and anti-interference capability of oxytetracycline detection are improved.

This utility model relates to the field of life science technology, and in particular to a nucleic acid extraction device. The device includes a frame, a sample loading and waste liquid treatment mechanism, a oscillating magnetic heating mechanism, a control device, and a screen interaction system. The sample loading and waste liquid treatment mechanism achieves precise three-dimensional positioning through horizontal and vertical motion guides and vertical moving components. It includes parallel large and small pistons, allowing for flexible switching between different volume pipetting and sharing a common connector. The oscillating magnetic heating mechanism integrates oscillation, heating, and magnetic attraction functions, and works with various sizes of pipette tips and reagent kits to complete sample processing. The extraction process is automated through the control device, linking all mechanisms to automatically complete the entire process, including sample lysis, solid-liquid separation, magnetic bead purification, and elution. This application automates nucleic acid extraction, improving operational efficiency and extraction results, and is suitable for nucleic acid extraction from plant tissues, animal tissues, blood, plasmids, and other samples.