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Method for establishing hematopoietic stem cells bank by extracting hematopoietic cells from placenta tissues

A technology of hematopoietic stem cells and placental tissue, applied in the direction of blood/immune system cells, animal cells, tissue culture, etc., can solve the problems of high cost and time-consuming, shorten the time, reduce the incidence of pollution, and save lives.

Inactive Publication Date: 2003-04-02
上海厚东生物科技有限公司
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Problems solved by technology

Experiments have proved that umbilical cord blood cells can be expanded tens of thousands of times by using various cell stimulating factors and appropriate culture conditions, but the problem is that this expansion takes time and is expensive, and more importantly, due to the expansion, hematopoietic stem cells Differentiation also occurs, and the results of clinical application show that there is not much difference before and after the expansion of cord blood cells for transplantation

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  • Method for establishing hematopoietic stem cells bank by extracting hematopoietic cells from placenta tissues
  • Method for establishing hematopoietic stem cells bank by extracting hematopoietic cells from placenta tissues
  • Method for establishing hematopoietic stem cells bank by extracting hematopoietic cells from placenta tissues

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Embodiment Construction

[0033] The technical scheme of the present invention includes a method for extracting tissue cells from placental tissue. This method includes (1) how to store data about placenta and cord blood in a systematic and orderly networked computer. (2) How to treat the placenta aseptically to avoid all possible bacteria and mold contamination. (3) How to clear the placenta and umbilical cord blood cells from the mother's blood. (4) How to separate placental tissue cells by mechanical methods. (5) How to separate placental tissue cells by enzymatic digestion. (6) How to use CD34 monoclonal antibody-magnetic beads method to purify placental tissue stem cells. (7) How to freeze placental tissue cells for a long time. And how to use cord blood and placental tissue hematopoietic stem / progenitor cells in combination, from veins, arteries or directly into the bone marrow cavity or corresponding tissues; provide a method for placental tissue cell transplantation that can be derived from allogen...

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Abstract

A process for extracting hemopoietic stem cells from the placenta of healthy puerperant to create hemopoistic stem cell bank features that the mechanical, enzyme digestive and monoclonal antibody-magnetic bead method is used to extract blood stem cells from placenta, and concentrate and purify them. Its advantages are high activity and high concentration.

Description

Technical field [0001] The present invention relates to a method for establishing a hematopoietic stem cell bank belonging to the fields of international patent classification C12N and A61K, in particular to a new method for establishing hematopoietic stem cell bank by extracting hematopoietic stem cells from placental tissue. Background technique [0002] The fertilized egg cells of the human body can develop into embryonic cells in the uterus. These cells have the ability to differentiate into various tissue cells, such as nerve cells, muscle cells, liver cells, blood cells, etc., so this cell is also called pluripotent stem cell . The cells that are directed to differentiate into a certain tissue are also called directed stem cells, such as hematopoietic stem cells and neural stem cells. These cells can continue to differentiate into specific cells or tissues, and blood hematopoietic stem cells can continue to differentiate into red blood cells, white...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/50A61P7/06C12N5/0789
Inventor 周胜利宋剑秋胡玉兰
Owner 上海厚东生物科技有限公司
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