78 results about "Immunofluorescence staining" patented technology

The invention discloses a serum migration body identification method which comprises the following steps: extracting a serum migration body from serum by performing multiple centrifugal treatment on the serum; carrying out immunofluorescence staining treatment on the serum migration body to obtain an immunofluorescence stained serum migration body; and carrying out imaging treatment on the serum migration body subjected to immunofluorescence staining by utilizing a super-resolution fluorescence microscope SIM to identify the serum migration body. Whether an extracellular vesicle in serum is a migration body or not is identified from the two aspects of biochemistry and shape, so that the defect that the serum migration body is identified only from the shape in the prior art is overcome.

The invention relates to a circulating tumor cell enrichment, separation and dyeing integrated system and application thereof.The circulating tumor cell enrichment, separation and dyeing integrated system is composed of a bottom plate module, a platform module, a multi-pipette module, a power module and a shell module; the platform module comprises a sample tube rotating and uniformly mixing module, a magnetic adsorption module, a reagent module, a waste liquid pool module and a gun head module, and the sample tube rotating and uniformly mixing module, the magnetic adsorption module and the reagent module are fixedly mounted on a bottom plate I in sequence. The CTC separation and dyeing integrated system is developed on the basis of the immunomagnetic bead technology, enrichment separation and immunofluorescent dyeing of CTC can be automatically completed by running a program, manual operation errors are effectively avoided through a programmed process, and the CTC separation and dyeing integrated system is particularly suitable for clinical CTC detection. The system is also suitable for other enrichment and separation applications based on an immunomagnetic bead technology, including but not limited to enrichment and separation of biological macromolecules such as cells, exosomes, proteins and the like.

The invention provides an immunofluorescence detection method and system for dynamically detecting mouse uterus structure development based on multiple time points, and immunofluorescence staining comprises the steps of sheet unfolding, dewaxing, repairing, cell membrane punching, sealing, primary antibody treatment and secondary antibody treatment. The system comprises a sample pretreatment control module, a multi-channel confocal microscope imaging module and a data analysis module integrating fluorescence intensity calculation, Pearson co-localization analysis and a Western blot data integration algorithm. Through a multi-time-point and multi-index immunofluorescence detection method, dynamic and systematic data support is provided for mouse uterus development mechanism research, and an application basis is provided for fundamental research of reproductive biology and construction of related disease models.

The invention discloses a rapid degreasing method for a biological tissue sample, which comprises the following steps: sequentially carrying out fixing, tissue dehydration, azeotropic degreasing, tissue rehydration and other treatments on the sample, and the degreasing solution is composed of alcohols and hydrocarbons according to a mass ratio of 1: 1-1: 2. According to the rapid degreasing method provided by the invention, the lipid in the tissue is efficiently extracted through mixed azeotropy of the alcohol and hydrocarbon organic reagents, so that the tissue is transparent and transparent; the problem of poor degreasing in tissues is solved by utilizing the high permeability characteristic of the micromolecular organic reagent; the dissolving capacity to phospholipid and cholesterol is improved by mixing organic reagents; through azeotropy, the degreasing efficiency is improved, the degreasing time is shortened, the internal permeability effect of the tissue is improved, and internal and external uniform permeability of the tissue is realized; by adjusting the components of the organic reagent, boiling rapid degreasing at low temperature is realized, and the retention of a fluorescence signal is improved; the tissue treated by the method is transparent and small in deformation, a fluorescent protein signal can be maintained, and the immunofluorescence staining effect can be improved.

The invention discloses application of 4-isopropyltoluene to improvement of depression-like behaviors of mice. An experiment adopts a chronic constraint stress method to construct a depression model, through behavioral detection such as a sucrose preference test, a tail suspension test and a novel inhibition ingestion test, and in combination with molecular biology means such as immunofluorescent staining and Western blot, the anti-depression effect and potential mechanism of 4-isopropyltoluene are systematically evaluated, and the anti-depression effect and potential mechanism of 4-isopropyltoluene are evaluated. Results show that 4-isopropyltoluene plays a significant anti-depression role by inhibiting neuroinflammation, enhancing glial cell support function and activating AKT signal pathway, and has the potential of being developed into a novel anti-depression candidate drug.

The invention discloses a multi-immunofluorescence staining cell target detection method and device based on environmental information fusion. The method comprises the following steps: 1) constructing an image detection framework combined with environmental information fusion; 2) detecting network anchor point adjustment and positioning; 3) accurately positioning a feature map and generating cells in a classified manner; and 4) performing feature accurate positioning target detection based on environmental information guidance. According to the method, target category image target detection based on environment information conversion is realized through a feature vector accurate positioning form based on environment information of a DAPI image.

The invention discloses an application of a compound fresh bamboo juice in a guinea pig asthma model, and relates to the field of medicines, a Hartley albino guinea pig is adopted to construct an OVA induced asthma model, and the model is verified to be successfully constructed through evaluation indexes; evaluating the efficacy of the successfully constructed guinea pig asthma model by using different doses of compound fresh bamboo juice, and evaluating the efficacy of the guinea pig asthma model by scoring guinea pig capsaicin stimulation cough inducing times, alveolar lavage fluid leukocyte number, inflammatory factor content, lung tissue hydroxyproline content and behavioral signs. And evaluating the treatment effect of the compound fresh bamboo juice through Western blot detection, lung histopathology and immunofluorescence staining indexes. In a guinea pig asthma model, high-dose and medium-dose groups of the compound fresh bamboo juice can be used for remarkably reducing the number of cough, reducing the number of white blood cells in alveolar lavage fluid and the level of inflammatory factors and inhibiting pulmonary fibrosis, and a good treatment effect is shown. The results provide powerful support for potential application of the compound fresh bamboo juice in asthma treatment.

The utility model belongs to the technical field of immunofluorescent staining, and particularly relates to an immunofluorescent staining tool kit which comprises a base, the cleaning module is detachably mounted on the base, and the cleaning module is used for cleaning the used cover glass; the dyeing module is detachably mounted on the cleaning module, and the dyeing module is used for dyeing the cover glass; and the cover plate is detachably mounted on the dyeing module. The utility model is convenient to use and can ensure the dyeing effect. The evaporation of the reagent in the long-time incubation process can be reduced, so that the dyeing effect is more stable. In addition, independent dyeing during simultaneous incubation and transfer of a plurality of cover glass can be guaranteed, and mutual confusion can be avoided. The cleaning module can simultaneously meet different requirements of rapid cleaning and long-time cleaning of the cover glass, reagent adding can be completed at a time, and the cumbersome step of replacing reagents for multiple times is omitted. Meanwhile, due to the detachable design, all the components can be cleaned and dried more easily, and the cover glass can be taken and placed conveniently, and cleaning liquid can be replaced conveniently.

The invention relates to the technical field of antibody detection, in particular to a recombinant antigen and application of a transfected living cell immunofluorescence method of the recombinant antigen in anti-myelin oligodendroglia glycoprotein IgG antibody detection. The method comprises the following steps: constructing a myelin sheath oligodendroglia glycoprotein expression plasmid, with an expression plasmid vector being pcdna3.1-target gene-linker-* 3Flag-P2A-EGFP, an amino acid sequence of the expression plasmid gene being as shown in SEQ ID NO: 1, a base sequence of the expression plasmid gene being as shown in SEQ ID NO: 2, carrying out cell transfection to make the expression plasmid enter a target cell, culturing for 16-28 h to obtain a cell immobilized with the expression plasmid, and purifying the cell to obtain the myelin sheath oligodendroglia glycoprotein. The kit can be used for detecting the anti-myelin oligodendroglia glycoprotein IgG antibody by an immunofluorescence staining method. The problems of antigen spatial conformation change, epitope shielding or cell shrinkage of cells are solved, and the detection sensitivity is improved.

The utility model relates to a staining module and an immunofluorescence staining instrument, and the staining module comprises a mechanical arm module which is provided with a liquid suction and injection needle capable of moving along a preset direction; the glass slide module is provided with a cleaning groove and a plurality of glass slide grooves, the glass slide grooves are used for bearing glass slides, reaction holes are formed in the glass slides, and cleaning liquid is stored in the cleaning groove; when the glass slides are arranged in one or more glass slide grooves, the liquid suction and injection needle can enter the reaction hole and the cleaning groove of each glass slide in the movement process of the liquid suction and injection needle. After the liquid suction and injection needle enters the reaction hole of one glass slide and before the liquid suction and injection needle enters the reaction holes of the other glass slides, the liquid suction and injection needle enters the cleaning tank firstly, and the liquid suction and injection needle is cleaned through the cleaning liquid in the cleaning tank, so that pollution sources possibly carried by the liquid suction and injection needle are removed; the influence on washing among the glass slides is avoided, and the dyeing quality of a sample is improved.

This invention discloses a high-throughput automated immunofluorescence staining device and its staining method. The device includes a liquid injection motion module, a reaction chamber module, a supply and waste collection module, and a control system. The liquid injection motion module includes an X-axis beam and a pipetting and auxiliary material robotic arm, which are driven in parallel to achieve sequential operation. The reaction chamber module includes a temperature-controlled station, a low-temperature reagent storage unit, and an auxiliary material supply unit. The supply and waste collection module includes a waste removal and cleaning unit and an auxiliary material waste collection unit. This invention achieves a 96-station array through a temperature-controlled reaction station, combined with the dual-drive parallel operation of the pipetting and auxiliary material robotic arms, eliminating action waiting time and significantly improving throughput. The use of a closed semiconductor cooling component linked with an automatic flip-top mechanism ensures uniform reagent activity over a long period. A plate-mounted small vacuum pump drives a rigid pipeline with deep-hole waste suction, combined with high-flow-rate wide-area spraying, to thoroughly remove waste liquid and reduce cross-contamination. This achieves full-process automation, significantly improving staining quality and efficiency.

This application belongs to the field of biomaterials, specifically relating to an antibacterial polymeric derivative of F127 with terminal modification of acetyl bromide, its preparation method, and its application. This application obtains F127AcBr by terminal modification of polymeric F127 with acetyl bromide via an acylation reaction. Its good antibacterial effect and biocompatibility were verified in vitro using plate antibacterial assays against Staphylococcus aureus and Escherichia coli, as well as live / dead staining assays of L929 cells and HUVEC cells. This application optimized the concentration of F127AcBr and loaded it into an MS hydrogel system. Excellent biocompatibility was confirmed in a diabetic mouse wound model using HE staining of mouse organs. Immunofluorescence staining against Staphylococcus aureus further confirmed the good antibacterial activity of F127AcBr.

The invention discloses a method for preparing a vascular organ chip from human umbilical artery stem cells, and belongs to the field of biomedical engineering and tissue engineering. The method comprises the following steps: separating and culturing human umbilical artery stem cells to the third generation; preparing a PDMS micro-fluidic chip containing a liquid inlet channel, a liquid outlet channel, a growth channel and a central culture chamber; a blood vessel precursor aggregate is obtained through cell digestion, aggregate construction, mesoderm induction and blood vessel pedigree induction, the blood vessel precursor aggregate is inoculated into a Coll-1-Matrigel gel modified chip through a two-step method, and dynamic perfusion culture is conducted in combination with a culture medium containing VEGF-A and FGF-2. And finally, the structure and the function of the vascular organ are verified through immunofluorescent staining and fluorescent microbead perfusion. The problems that in the prior art, cell sources are limited, culture conditions are single and the like are solved, the high angiogenesis potential of the human umbilical artery stem cells and a dynamic culture system are utilized to construct a high-bionic-degree vascular network, and the method can be applied to drug screening, vascular pathological model construction and regenerative medicine research.

The invention discloses an immunofluorescence staining method for karyocytes in blood. When immunofluorescence staining is carried out, polylysine is used for treating a cell slide, and then the cell is fixed and smeared and dried, so that the cell and the slide are attached more tightly. The problem that cells fall off in the cleaning process can be effectively avoided. Meanwhile, non-blood cells in the blood sample can be effectively reserved.

The utility model discloses tissue immunofluorescence bleaching and dyeing equipment, which relates to the technical field of immunofluorescence and comprises a bottom plate, a cover plate is arranged on the upper side of the bottom plate, a groove is arranged on the upper side surface of the cover plate, the bottom end of the cover plate penetrates into the groove and is provided with a drain hole, and a water blocking plug is arranged on the upper side surface of the bottom plate and penetrates through the drain hole. According to the utility model, waste of tissues during slicing is avoided, a plurality of samples can be treated at the same time, the tissues can be dyed more fully and thoroughly, and non-specific dyeing can be reduced.

The invention discloses an absolute quantitative detection method for urine exfoliated podocyte, relates to the technical field of biomedical detection, and aims to solve the problem that the urine exfoliated podocyte cannot be accurately and absolutely quantified due to uncertain cell loss in a sample treatment process in the prior art. The method comprises the following steps: before carrying out any physical treatment on a urine sample, adding a known number of internal standard reference substances with physical characteristics similar to those of cells into the urine sample, carrying out co-enrichment treatment on a mixed sample containing target podocytes and the internal standard reference substances, and preparing a cell slide by adopting a standardized slide preparation technology; and carrying out podocyte specific immunofluorescence staining on the slide. Through the design of internal standard preposition and whole-course synchronous calibration, the cell loss error in the operation process is effectively overcome, the traditional semi-quantitative detection is improved into accurate and repeatable absolute quantification, and the reliability and clinical application value of a detection result are remarkably improved.

The application discloses a method for constructing a cell membrane fusion model induced by a novel coronavirus-like particle, and belongs to the technical field of biology. The method comprises the following steps: constructing a virus-like particle containing a SARS-CoV-2 spike protein gene and an RNA packaging sequence PS9, using HEK-293T cells as packaging cells, co-expressing structural proteins M, E, N and S protein, and realizing assembly of VLPs without replication ability. Subsequently, the VLPs are used to infect immortalized type II alveolar epithelial cells, S protein expression is induced and cell-cell membrane fusion is mediated without the need of BSL-3 laboratory conditions. The formation of fused giant cells can be directly observed through ZO-1 protein immunofluorescence staining, the model is stable, has high biological safety, and is repeatable in vitro. The application overcomes the safety risk of live virus infection and the authenticity problem of a transient transfection model, can be used for research on a SARS-CoV-2 virus membrane fusion mechanism, and has a good application prospect.

According to the isolated culture identification and key marker detection method for the down producing goat hair follicle stem cells, trypsin and EDTA are used for jointly digesting skin tissue, and high-activity cells are efficiently obtained; a culture medium containing various growth factors is adopted for culture, and cell proliferation and stemness maintenance are promoted. And identifying and integrating morphological observation, growth curve determination and clone formation experiment to accurately judge the characteristics of the cells. Immunofluorescent staining and real-time fluorescent quantitative PCR are applied to key marker detection, and expression of markers such as CK15, CD34 and beta1 integrin is detected with high sensitivity. The method is high in separation efficiency, accurate in identification and sensitive in marker detection, can provide sufficient high-quality cells for the research on the hair follicle stem cells of the cashmere goat, assists in deeply understanding the biological characteristics and the regulation mechanism of the cells, and is of great significance for improving the yield and quality of cashmere and promoting the development of related biotechnologies.

The present application relates to the technical field of edge detection algorithm, and particularly relates to an end-to-end cell aging state prediction method based on nuclear membrane morphology, which comprises the following steps: step one, collecting a fluorescence image of human fibroblasts subjected to immunofluorescence staining through a fluorescence microscope; step two, performing data enhancement processing on nuclear membrane data, amplifying training data, and improving model generalization capability; step three, generating a prediction of nuclear membrane segmentation mask through a deep neural network, and obtaining mask information; and step four, simultaneously inputting the mask information into an ellipticity module, a smoothness module and a concave degree module for multi-angle calculation and evaluation. The present application evaluates nuclear membrane morphology changes from multiple angles to meet the growing demand for quantification of cell aging state in the field.

The invention relates to a construction method of a bionic endometrial organ, which comprises the following steps: mixing three primary cells to obtain a mixed cell precipitate, and adding the mixed cell precipitate into a bionic organ culture medium; precipitating and resuspending the mixed cells to obtain a cell suspension; and adding Matrigel, and transferring into a constant-temperature cell culture box for culturing, so as to obtain the bionic endometrial organ. The regeneration promoting effect of the bionic endometrial organ provided by the invention is obviously better than that of transplanted pure stromal cells and pure epithelial organ groups, and the endometrial organ of the bionic cells has a huge application value in the aspect of tissue regeneration. According to the construction method of the intrauterine adhesion disease model, a fibrosis inducing factor TGF beta 1 is added into a bionic endometrial organ, immunofluorescent staining of a fibrosis marker alpha SMA is carried out, and the intrauterine adhesion disease model is obtained. By reproducing the pathological change of the intrauterine adhesion inner membrane, the model can be used as an intrauterine adhesion pathological model for disease pathological research or drug screening research.

The invention discloses a soft tissue thick slice preparation and high-resolution multicolor fluorescent three-dimensional imaging method. The method comprises the following steps: step 1, carrying out fixation and freezing protection treatment on a sample; step 2, putting the sample treated in the step 1 into an embedding medium, incubating and embedding to form an embedded block, and freezing and slicing the embedded block; the embedding medium comprises gelatin with a mass concentration of 8%, polyvinylpyrrolidone with a mass concentration of 2%, sucrose with a mass concentration of 20% and the balance of a phosphate buffer solution; step 3, unfreezing the slices in the step 2 in a room temperature environment, then carrying out pretreatment, antibody incubation and immunofluorescence staining, and carrying out pretreatment, antibody incubation and cell nucleus labeling in a dark environment; 4, performing three-dimensional imaging on the sample sliced in the step 3 by adopting a turntable confocal microscope to obtain three-dimensional data of tissue fluorescence distribution; however, the polyvinylpyrrolidone inhibits the formation and growth of ice crystals in the freezing process of the embedded block, and ensures the accuracy of the dyeing effect.

The invention belongs to the technical field of antibody detection, and particularly relates to a multi-subtype antigen co-transfected cell immunofluorescence staining method and application. The method is established for the purpose of a CBA detection method, the basic principle is similar to that of an overexpression antigen cell immunofluorescence method for existing anti-GFAP-IgG antibody detection, and the difference is that three GFAP subtypes are adopted for cotransfection design, antigen subtypes are covered, and the problem of leak detection is avoided. According to the invention, the high-quality expression of the antigen is realized by self-constructing the plasmid based on the pCDH lentiviral vector. According to the invention, by optimizing the schemes of fixation, transparent preservation and the like after cell transfection culture, the dyeing performance is enhanced, and the preservation effect is improved.

The invention relates to the technical field of deep learning, and discloses a prediction method and system for a liver cancer immunotherapy effect. The method comprises the following steps: carrying out multiple immunofluorescence staining pretreatment on a clinical sample, and collecting forward scattering data, lateral scattering data and multi-channel fluorescence data of mononuclear cells to obtain a cell expression initial data matrix; performing standardization processing to obtain a multi-dimensional feature matrix expressed by the immune factors; performing Bayesian network analysis, calculating a conditional probability relation and a mutual information value between immune cell factors, and constructing an immune factor association network; performing anomaly detection to obtain an immune factor expression anomaly detection result; and performing minimum tail boundary analysis on the immune factor expression anomaly detection result, solving an optimal risk boundary through a semi-definite programming model, and outputting immune factor anomaly quantitative evaluation data, thereby realizing quantitative risk evaluation of the prediction result and refined grading of the prediction result.

The application discloses a kind of based on non-diffracting airy beam intrahepatic blood vessel three-dimensional imaging method, belong to intrahepatic blood vessel three-dimensional imaging technical field, solve the problem of insufficient accuracy and detection sensitivity of existing detection method, method includes by liver transplantation or biopsy surgery obtains liver tissue, liver tissue is fixed using 4%PFA processing, liver tissue is bleached using H2O2Solution, antigen repair is carried out to liver tissue using FLASH solution, using goat serum blocking solution to the liver tissue after antigen repair blocking treatment;Liver tissue immunostaining treatment, liver tissue dehydration treatment, based on non-diffracting airy beam and high-throughput microscope to the liver tissue after processing imaging, obtain the three-dimensional imaging picture of liver tissue;The application is immunofluorescence staining and transparent treatment to liver tissue, so that laser can smoothly penetrate tissue and carry out three-dimensional imaging, can realize single wide-range image capture.

This invention discloses a monoclonal antibody against Oit3 protein in mouse hepatic sinusoidal endothelial cells or its antigen-binding fragment and its applications, belonging to the fields of biotechnology and medical immunology. The monoclonal antibody or its antigen-binding fragment comprises a heavy chain variable region and a light chain variable region. The heavy chain variable region contains HCDR1, HCDR2, and HCDR3 with amino acid sequences as shown in SEQ ID NO. 3~SEQ ID NO. 5; the light chain variable region contains LCDR1, LCDR2, and LCDR3 with amino acid sequences as shown in SEQ ID NO. 8~SEQ ID NO. 10. This monoclonal antibody and the recombinant fluorescent antibody exhibit high affinity and high specificity, and can be effectively applied to enzyme-linked immunosorbent assay (ELISA), Western blotting, and immunofluorescence detection. They can serve as important antibody tools for basic research on mouse hepatic sinusoidal endothelial cells and for exploring the mechanisms of liver diseases. Based on its variable region sequence, a recombinant fluorescent antibody, Oit3-scFv-GFP, was also constructed. This recombinant fluorescent antibody enables one-step direct immunofluorescence staining, providing a convenient tool for in situ visualization of mouse hepatic sinusoidal endothelial cells.

The invention discloses application of Dectin-1 as a biomarker in preparation of a renal fibrosis diagnostic reagent or kit, and belongs to the field of biological medicine. And Dectin-1 is a member of a pattern recognition receptor C-type lectin family. According to the invention, Dectin-1 immunofluorescent staining is carried out on kidney tissues of patients with chronic kidney diseases, and observation shows that the number of Dectin-1 + cells in fibrosis kidneys is more. Dectin-1 immunofluorescence staining is further carried out in the kidney tissue of the patient with the IgA nephropathy, and it is found that the number of Dectin-1 + cells in the kidney tissue of the patient with the IgA nephropathy is in positive correlation with the renal fibrosis degree. The results show that the reagent for detecting the expression level of the Dectin-1 in the kidney cells can be used for preparing the kit for diagnosing the kidney fibrosis, and the diagnosis result has high sensitivity and specificity.

The application discloses a kind of northern Shaanxi white cashmere goat muscle satellite cell separation and purification method, the present application uses northern Shaanxi white cashmere goat fetus dorsal longest muscle tissue as test material, by aseptic pretreatment, tissue block inversion adherent culture, primary subculture culture Combination of compound technology of hierarchical differential adherent purification, stably obtain the 5th generation muscle satellite cell.The present application optimizes and adapts culture system and purification process, overcomes the defects of low cell yield, serious interference of mixed cells, activity and poor purity of traditional preparation method.At the same time, the present application also adopts RT-PCR molecular identification and immunofluorescence staining combined with multi-level verification method, from gene and protein level Bidirectional verification cell specificity and differentiation potential, accurately determine the biological properties of cell.The present application process is simple, good stability and repeatability, can efficiently prepare northern Shaanxi white cashmere goat muscle satellite cell, provides important technical support for cashmere goat germplasm resource conservation, muscle development mechanism and muscle regeneration related research.

The utility model discloses a portable cleaning and antibody incubation device for floating piece type immunofluorescence staining. The portable cleaning and antibody incubation device comprises a net-shaped accommodating cylinder and an extension part, wherein the accommodating barrel is cylindrical, and the bottom wall of the accommodating barrel is flush with the bottom wall of the pore plate for a certain distance; the extension part is of an annular design and is arranged at the top end of the accommodating cylinder in a winding manner; and the outer cover body is detachably arranged on the outer wall of the containing cylinder in a sleeving manner. According to the portable cleaning and antibody incubation device for floating piece type immunofluorescence staining, the containing cylinder and the outer cover bodies with different specifications are arranged, so that time and labor are saved in the processes of rinsing brain pieces and staining in the immunofluorescence staining, repeated piece picking is not needed, at least 80%-90% of experimental operation time can be saved, and the working efficiency is improved. And the integrity of the brain slice can be ensured.

This disclosure provides an image detection method, apparatus, electronic device, and storage medium. The image detection method includes: acquiring a multiplex immunofluorescence image to be tested, wherein the multiplex immunofluorescence image is imaging data after multiplex immunofluorescence staining of a tissue sample to be tested; performing tissue segmentation based on the multiplex immunofluorescence image to obtain a tissue region image; and performing image sharpness detection on the tissue region image to obtain a detection result. The image detection method provided by this application can effectively eliminate background noise interference in multiplex immunofluorescence images caused by staining at different locations in the image, greatly reducing the proportion of background in the input image for subsequent image detection, and improving the accuracy and reliability of the image detection results.

The invention provides application of MCT2 protein as a marker in preparation of products for ovarian cancer stem cell identification and treatment effect evaluation, and belongs to the technical field of biomedicine. According to the invention, primary ovarian cancer cells are obtained, an OCSC model is established by screening and culturing, an inhibitor with MCT2Ki specificity is adopted for intervention, an OCSC marker is identified by adopting flow cytometry and qRT-PCR, and the expression level and cell localization of MCT2 are detected by adopting a qRT-PCR method and an immunofluorescence staining method. A stem cell balling experiment and a matrigel migration chamber experiment are adopted to verify the influence of the MCT2 inhibitor on the dryness and invasiveness of the OCSC, and verify that the MCT2 is in a high expression state in the primary OCSC, and the dryness and invasiveness of ovarian cancer cells can be reduced by inhibiting the MCT2, so that the MCT2 can become a new target for treating high-grade ovarian cancer.