54 results about "Immunofluorescence staining" patented technology

The invention relates to a circulating tumor cell enrichment, separation and dyeing integrated system and application thereof.The circulating tumor cell enrichment, separation and dyeing integrated system is composed of a bottom plate module, a platform module, a multi-pipette module, a power module and a shell module; the platform module comprises a sample tube rotating and uniformly mixing module, a magnetic adsorption module, a reagent module, a waste liquid pool module and a gun head module, and the sample tube rotating and uniformly mixing module, the magnetic adsorption module and the reagent module are fixedly mounted on a bottom plate I in sequence. The CTC separation and dyeing integrated system is developed on the basis of the immunomagnetic bead technology, enrichment separation and immunofluorescent dyeing of CTC can be automatically completed by running a program, manual operation errors are effectively avoided through a programmed process, and the CTC separation and dyeing integrated system is particularly suitable for clinical CTC detection. The system is also suitable for other enrichment and separation applications based on an immunomagnetic bead technology, including but not limited to enrichment and separation of biological macromolecules such as cells, exosomes, proteins and the like.

The invention provides an immunofluorescence detection method and system for dynamically detecting mouse uterus structure development based on multiple time points, and immunofluorescence staining comprises the steps of sheet unfolding, dewaxing, repairing, cell membrane punching, sealing, primary antibody treatment and secondary antibody treatment. The system comprises a sample pretreatment control module, a multi-channel confocal microscope imaging module and a data analysis module integrating fluorescence intensity calculation, Pearson co-localization analysis and a Western blot data integration algorithm. Through a multi-time-point and multi-index immunofluorescence detection method, dynamic and systematic data support is provided for mouse uterus development mechanism research, and an application basis is provided for fundamental research of reproductive biology and construction of related disease models.

The invention discloses a rapid degreasing method for a biological tissue sample, which comprises the following steps: sequentially carrying out fixing, tissue dehydration, azeotropic degreasing, tissue rehydration and other treatments on the sample, and the degreasing solution is composed of alcohols and hydrocarbons according to a mass ratio of 1: 1-1: 2. According to the rapid degreasing method provided by the invention, the lipid in the tissue is efficiently extracted through mixed azeotropy of the alcohol and hydrocarbon organic reagents, so that the tissue is transparent and transparent; the problem of poor degreasing in tissues is solved by utilizing the high permeability characteristic of the micromolecular organic reagent; the dissolving capacity to phospholipid and cholesterol is improved by mixing organic reagents; through azeotropy, the degreasing efficiency is improved, the degreasing time is shortened, the internal permeability effect of the tissue is improved, and internal and external uniform permeability of the tissue is realized; by adjusting the components of the organic reagent, boiling rapid degreasing at low temperature is realized, and the retention of a fluorescence signal is improved; the tissue treated by the method is transparent and small in deformation, a fluorescent protein signal can be maintained, and the immunofluorescence staining effect can be improved.

The invention discloses an application of a compound fresh bamboo juice in a guinea pig asthma model, and relates to the field of medicines, a Hartley albino guinea pig is adopted to construct an OVA induced asthma model, and the model is verified to be successfully constructed through evaluation indexes; evaluating the efficacy of the successfully constructed guinea pig asthma model by using different doses of compound fresh bamboo juice, and evaluating the efficacy of the guinea pig asthma model by scoring guinea pig capsaicin stimulation cough inducing times, alveolar lavage fluid leukocyte number, inflammatory factor content, lung tissue hydroxyproline content and behavioral signs. And evaluating the treatment effect of the compound fresh bamboo juice through Western blot detection, lung histopathology and immunofluorescence staining indexes. In a guinea pig asthma model, high-dose and medium-dose groups of the compound fresh bamboo juice can be used for remarkably reducing the number of cough, reducing the number of white blood cells in alveolar lavage fluid and the level of inflammatory factors and inhibiting pulmonary fibrosis, and a good treatment effect is shown. The results provide powerful support for potential application of the compound fresh bamboo juice in asthma treatment.

The utility model belongs to the technical field of immunofluorescent staining, and particularly relates to an immunofluorescent staining tool kit which comprises a base, the cleaning module is detachably mounted on the base, and the cleaning module is used for cleaning the used cover glass; the dyeing module is detachably mounted on the cleaning module, and the dyeing module is used for dyeing the cover glass; and the cover plate is detachably mounted on the dyeing module. The utility model is convenient to use and can ensure the dyeing effect. The evaporation of the reagent in the long-time incubation process can be reduced, so that the dyeing effect is more stable. In addition, independent dyeing during simultaneous incubation and transfer of a plurality of cover glass can be guaranteed, and mutual confusion can be avoided. The cleaning module can simultaneously meet different requirements of rapid cleaning and long-time cleaning of the cover glass, reagent adding can be completed at a time, and the cumbersome step of replacing reagents for multiple times is omitted. Meanwhile, due to the detachable design, all the components can be cleaned and dried more easily, and the cover glass can be taken and placed conveniently, and cleaning liquid can be replaced conveniently.

This invention discloses a high-throughput automated immunofluorescence staining device and its staining method. The device includes a liquid injection motion module, a reaction chamber module, a supply and waste collection module, and a control system. The liquid injection motion module includes an X-axis beam and a pipetting and auxiliary material robotic arm, which are driven in parallel to achieve sequential operation. The reaction chamber module includes a temperature-controlled station, a low-temperature reagent storage unit, and an auxiliary material supply unit. The supply and waste collection module includes a waste removal and cleaning unit and an auxiliary material waste collection unit. This invention achieves a 96-station array through a temperature-controlled reaction station, combined with the dual-drive parallel operation of the pipetting and auxiliary material robotic arms, eliminating action waiting time and significantly improving throughput. The use of a closed semiconductor cooling component linked with an automatic flip-top mechanism ensures uniform reagent activity over a long period. A plate-mounted small vacuum pump drives a rigid pipeline with deep-hole waste suction, combined with high-flow-rate wide-area spraying, to thoroughly remove waste liquid and reduce cross-contamination. This achieves full-process automation, significantly improving staining quality and efficiency.

This application belongs to the field of biomaterials, specifically relating to an antibacterial polymeric derivative of F127 with terminal modification of acetyl bromide, its preparation method, and its application. This application obtains F127AcBr by terminal modification of polymeric F127 with acetyl bromide via an acylation reaction. Its good antibacterial effect and biocompatibility were verified in vitro using plate antibacterial assays against Staphylococcus aureus and Escherichia coli, as well as live / dead staining assays of L929 cells and HUVEC cells. This application optimized the concentration of F127AcBr and loaded it into an MS hydrogel system. Excellent biocompatibility was confirmed in a diabetic mouse wound model using HE staining of mouse organs. Immunofluorescence staining against Staphylococcus aureus further confirmed the good antibacterial activity of F127AcBr.

The invention discloses a method for preparing a vascular organ chip from human umbilical artery stem cells, and belongs to the field of biomedical engineering and tissue engineering. The method comprises the following steps: separating and culturing human umbilical artery stem cells to the third generation; preparing a PDMS micro-fluidic chip containing a liquid inlet channel, a liquid outlet channel, a growth channel and a central culture chamber; a blood vessel precursor aggregate is obtained through cell digestion, aggregate construction, mesoderm induction and blood vessel pedigree induction, the blood vessel precursor aggregate is inoculated into a Coll-1-Matrigel gel modified chip through a two-step method, and dynamic perfusion culture is conducted in combination with a culture medium containing VEGF-A and FGF-2. And finally, the structure and the function of the vascular organ are verified through immunofluorescent staining and fluorescent microbead perfusion. The problems that in the prior art, cell sources are limited, culture conditions are single and the like are solved, the high angiogenesis potential of the human umbilical artery stem cells and a dynamic culture system are utilized to construct a high-bionic-degree vascular network, and the method can be applied to drug screening, vascular pathological model construction and regenerative medicine research.

The application discloses a method for constructing a cell membrane fusion model induced by a novel coronavirus-like particle, and belongs to the technical field of biology. The method comprises the following steps: constructing a virus-like particle containing a SARS-CoV-2 spike protein gene and an RNA packaging sequence PS9, using HEK-293T cells as packaging cells, co-expressing structural proteins M, E, N and S protein, and realizing assembly of VLPs without replication ability. Subsequently, the VLPs are used to infect immortalized type II alveolar epithelial cells, S protein expression is induced and cell-cell membrane fusion is mediated without the need of BSL-3 laboratory conditions. The formation of fused giant cells can be directly observed through ZO-1 protein immunofluorescence staining, the model is stable, has high biological safety, and is repeatable in vitro. The application overcomes the safety risk of live virus infection and the authenticity problem of a transient transfection model, can be used for research on a SARS-CoV-2 virus membrane fusion mechanism, and has a good application prospect.

According to the isolated culture identification and key marker detection method for the down producing goat hair follicle stem cells, trypsin and EDTA are used for jointly digesting skin tissue, and high-activity cells are efficiently obtained; a culture medium containing various growth factors is adopted for culture, and cell proliferation and stemness maintenance are promoted. And identifying and integrating morphological observation, growth curve determination and clone formation experiment to accurately judge the characteristics of the cells. Immunofluorescent staining and real-time fluorescent quantitative PCR are applied to key marker detection, and expression of markers such as CK15, CD34 and beta1 integrin is detected with high sensitivity. The method is high in separation efficiency, accurate in identification and sensitive in marker detection, can provide sufficient high-quality cells for the research on the hair follicle stem cells of the cashmere goat, assists in deeply understanding the biological characteristics and the regulation mechanism of the cells, and is of great significance for improving the yield and quality of cashmere and promoting the development of related biotechnologies.

The present application relates to the technical field of edge detection algorithm, and particularly relates to an end-to-end cell aging state prediction method based on nuclear membrane morphology, which comprises the following steps: step one, collecting a fluorescence image of human fibroblasts subjected to immunofluorescence staining through a fluorescence microscope; step two, performing data enhancement processing on nuclear membrane data, amplifying training data, and improving model generalization capability; step three, generating a prediction of nuclear membrane segmentation mask through a deep neural network, and obtaining mask information; and step four, simultaneously inputting the mask information into an ellipticity module, a smoothness module and a concave degree module for multi-angle calculation and evaluation. The present application evaluates nuclear membrane morphology changes from multiple angles to meet the growing demand for quantification of cell aging state in the field.

The invention discloses a soft tissue thick slice preparation and high-resolution multicolor fluorescent three-dimensional imaging method. The method comprises the following steps: step 1, carrying out fixation and freezing protection treatment on a sample; step 2, putting the sample treated in the step 1 into an embedding medium, incubating and embedding to form an embedded block, and freezing and slicing the embedded block; the embedding medium comprises gelatin with a mass concentration of 8%, polyvinylpyrrolidone with a mass concentration of 2%, sucrose with a mass concentration of 20% and the balance of a phosphate buffer solution; step 3, unfreezing the slices in the step 2 in a room temperature environment, then carrying out pretreatment, antibody incubation and immunofluorescence staining, and carrying out pretreatment, antibody incubation and cell nucleus labeling in a dark environment; 4, performing three-dimensional imaging on the sample sliced in the step 3 by adopting a turntable confocal microscope to obtain three-dimensional data of tissue fluorescence distribution; however, the polyvinylpyrrolidone inhibits the formation and growth of ice crystals in the freezing process of the embedded block, and ensures the accuracy of the dyeing effect.

The invention belongs to the technical field of antibody detection, and particularly relates to a multi-subtype antigen co-transfected cell immunofluorescence staining method and application. The method is established for the purpose of a CBA detection method, the basic principle is similar to that of an overexpression antigen cell immunofluorescence method for existing anti-GFAP-IgG antibody detection, and the difference is that three GFAP subtypes are adopted for cotransfection design, antigen subtypes are covered, and the problem of leak detection is avoided. According to the invention, the high-quality expression of the antigen is realized by self-constructing the plasmid based on the pCDH lentiviral vector. According to the invention, by optimizing the schemes of fixation, transparent preservation and the like after cell transfection culture, the dyeing performance is enhanced, and the preservation effect is improved.

The application discloses a kind of based on non-diffracting airy beam intrahepatic blood vessel three-dimensional imaging method, belong to intrahepatic blood vessel three-dimensional imaging technical field, solve the problem of insufficient accuracy and detection sensitivity of existing detection method, method includes by liver transplantation or biopsy surgery obtains liver tissue, liver tissue is fixed using 4%PFA processing, liver tissue is bleached using H2O2Solution, antigen repair is carried out to liver tissue using FLASH solution, using goat serum blocking solution to the liver tissue after antigen repair blocking treatment;Liver tissue immunostaining treatment, liver tissue dehydration treatment, based on non-diffracting airy beam and high-throughput microscope to the liver tissue after processing imaging, obtain the three-dimensional imaging picture of liver tissue;The application is immunofluorescence staining and transparent treatment to liver tissue, so that laser can smoothly penetrate tissue and carry out three-dimensional imaging, can realize single wide-range image capture.

This invention discloses a monoclonal antibody against Oit3 protein in mouse hepatic sinusoidal endothelial cells or its antigen-binding fragment and its applications, belonging to the fields of biotechnology and medical immunology. The monoclonal antibody or its antigen-binding fragment comprises a heavy chain variable region and a light chain variable region. The heavy chain variable region contains HCDR1, HCDR2, and HCDR3 with amino acid sequences as shown in SEQ ID NO. 3~SEQ ID NO. 5; the light chain variable region contains LCDR1, LCDR2, and LCDR3 with amino acid sequences as shown in SEQ ID NO. 8~SEQ ID NO. 10. This monoclonal antibody and the recombinant fluorescent antibody exhibit high affinity and high specificity, and can be effectively applied to enzyme-linked immunosorbent assay (ELISA), Western blotting, and immunofluorescence detection. They can serve as important antibody tools for basic research on mouse hepatic sinusoidal endothelial cells and for exploring the mechanisms of liver diseases. Based on its variable region sequence, a recombinant fluorescent antibody, Oit3-scFv-GFP, was also constructed. This recombinant fluorescent antibody enables one-step direct immunofluorescence staining, providing a convenient tool for in situ visualization of mouse hepatic sinusoidal endothelial cells.

The application discloses a kind of northern Shaanxi white cashmere goat muscle satellite cell separation and purification method, the present application uses northern Shaanxi white cashmere goat fetus dorsal longest muscle tissue as test material, by aseptic pretreatment, tissue block inversion adherent culture, primary subculture culture Combination of compound technology of hierarchical differential adherent purification, stably obtain the 5th generation muscle satellite cell.The present application optimizes and adapts culture system and purification process, overcomes the defects of low cell yield, serious interference of mixed cells, activity and poor purity of traditional preparation method.At the same time, the present application also adopts RT-PCR molecular identification and immunofluorescence staining combined with multi-level verification method, from gene and protein level Bidirectional verification cell specificity and differentiation potential, accurately determine the biological properties of cell.The present application process is simple, good stability and repeatability, can efficiently prepare northern Shaanxi white cashmere goat muscle satellite cell, provides important technical support for cashmere goat germplasm resource conservation, muscle development mechanism and muscle regeneration related research.

The utility model discloses a portable cleaning and antibody incubation device for floating piece type immunofluorescence staining. The portable cleaning and antibody incubation device comprises a net-shaped accommodating cylinder and an extension part, wherein the accommodating barrel is cylindrical, and the bottom wall of the accommodating barrel is flush with the bottom wall of the pore plate for a certain distance; the extension part is of an annular design and is arranged at the top end of the accommodating cylinder in a winding manner; and the outer cover body is detachably arranged on the outer wall of the containing cylinder in a sleeving manner. According to the portable cleaning and antibody incubation device for floating piece type immunofluorescence staining, the containing cylinder and the outer cover bodies with different specifications are arranged, so that time and labor are saved in the processes of rinsing brain pieces and staining in the immunofluorescence staining, repeated piece picking is not needed, at least 80%-90% of experimental operation time can be saved, and the working efficiency is improved. And the integrity of the brain slice can be ensured.

This disclosure provides an image detection method, apparatus, electronic device, and storage medium. The image detection method includes: acquiring a multiplex immunofluorescence image to be tested, wherein the multiplex immunofluorescence image is imaging data after multiplex immunofluorescence staining of a tissue sample to be tested; performing tissue segmentation based on the multiplex immunofluorescence image to obtain a tissue region image; and performing image sharpness detection on the tissue region image to obtain a detection result. The image detection method provided by this application can effectively eliminate background noise interference in multiplex immunofluorescence images caused by staining at different locations in the image, greatly reducing the proportion of background in the input image for subsequent image detection, and improving the accuracy and reliability of the image detection results.

The invention relates to the technical field of immunofluorescence detection, and particularly discloses a multiple immunofluorescence staining kit based on a multi-antibody stable premix solution as well as a preparation method and application of the multiple immunofluorescence staining kit. The kit comprises: a multi-antibody stable premix solution containing four fluorescence labeled antibodies targeting different antigens, each antibody being respectively coupled with a fluorescent dye of which the wavelength does not interfere with each other, and mixing the antibodies in a unified buffer system according to a preset proportion; the stable buffer system contains a phosphate buffer solution with the pH value of 7.2-7.6, trehalose, glycerol, bovine serum albumin and a nonionic surfactant; the antibody pretreatment unit comprises an antibody buffer replacement solution, a cross adsorbent and an Fc segment blocking antigen; and the sealing subpackaging pipe is used for low-temperature freeze-drying or liquid nitrogen cryopreservation. The kit can realize one-step co-staining of multi-target tissue slices, reduces operation steps, improves staining stability and signal specificity, and is suitable for pathological diagnosis, immune microenvironment analysis and biomedical research.

The invention discloses a method for researching the action mechanism of saikoside D on paclitaxel-induced peripheral neuropathy, and relates to the technical field of disease mechanism research and drug development. Comprising the following steps: performing continuous administration through intraperitoneal injection of paclitaxel, and establishing a paclitaxel-induced peripheral neuropathy animal model; a von Frey cellosilk experiment and a cold and hot plate experiment are adopted for behavioral detection, and mechanical pain threshold and cold and hot pain sense changes are evaluated; the morphological structure change of the sciatic nerve myelin sheath is observed through Luxol Fast Blue staining; and the expression level of the myelin sheath basic protein in the sciatic nerve is detected by using immunofluorescence staining and Western blotting technologies. According to the invention, a closed-loop research system is formed from animal model construction to molecular mechanism verification; based on behavioral detection, histological analysis and multiple dimensions of a molecular biological technology, the data reliability is ensured, and the curative effect of saikoside D can be comprehensively evaluated.

The invention provides a separation method and an identification method of bactrian camel testis interstitial cells, and belongs to the technical field of cell separation. The method comprises the following steps: mixing 0.08-0.12% of collagenase IV solution and 0.23-0.27% of trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) according to a volume ratio of 1: 2, digesting a bactrian camel testis tissue block, then carrying out hypotonic treatment purification to remove redundant germ cells, carrying out differential adhesion purification to remove a large amount of supporting cells, carrying out percoll density gradient centrifugation purification to remove a small amount of supporting cells, and carrying out freeze drying to obtain a finished product. And transferring the suspension with the percoll gradient of 34-60% into a new culture bottle to obtain the interstitial cells with the purity of more than or equal to 95%, and completing identification by combining morphological observation, immunofluorescence staining and secretion function detection. The separated bactrian camel testis interstitial cells can be used for double-hump reproductive performance research, and a foundation is laid for the reproductive performance of bactrian camels.

This invention discloses a fully automated immunofluorescence staining system and method, relating to the field of staining machine technology. The system includes an operating table equipped with a central control system and, through signal interaction with the central control system, a sample processing module, a reagent loading module, a cleaning needle module, a mounting module, a fluorescence imaging module, and an antigen loading module. The sample processing module is configured with several independent sample needle modules, each equipped with an independent Z-axis drive mechanism and an independent Y-axis drive mechanism. The central control system coordinates the operation of all modules, forming a fully automated closed loop from sample scanning, dilution, incubation, cleaning, secondary antibody loading to mounting, fluorescence imaging, and preliminary interpretation. This completely eliminates the limitations of traditional manual operation and the fragmented functionality of existing equipment, eliminating the need for manual coordination between experimental steps, significantly reducing reliance on manual labor, and effectively improving the detection efficiency of large batches of samples.

The application relates to the field of health assessment, and particularly discloses a ZNF281-based gastric cancer prognosis value evaluation system, which comprises a ZNF281 expression quantification module, which is used for detecting the protein / RNA expression level of ZNF281 in tumor tissue through immunohistochemistry, real-time fluorescent quantitative PCR or Western blot technology; a stemness feature and signal path analysis module, which is used for acquiring the protein expression or cell positive rate of stemness genes CD44 and SOX9; then evaluating the activation state of an IL6 / JAK / STAT3 path, including Western blot detection of a p-STAT3 / STAT3 ratio, ELISA determination of IL6 and CXCL5 cytokine levels; then analyzing the cancer-associated fibroblast infiltration in a tumor microenvironment, quantifying the CAF density through alpha-SMA immunofluorescence staining, and combining single-cell sequencing analysis of the interaction strength of a CXCL5 / CXCR2 signal shaft. The technical scheme of the application can integrate the expression amount of ZNF281, signal path activity and clinical pathological data, and precisely stratify the chemotherapy sensitivity and prognosis of gastric cancer patients.

The invention discloses a lymphatic endothelial cell cyst fluid extraction method for lymphatic deformity, and belongs to the technical field of biological cell extraction. Comprising the following steps: S1, sample collection: collecting 5-10 mL of cystic fluid of a patient with macrocystic lymphangial malformation, and placing the collected cystic fluid in a sterile container; s2, primary centrifugation: centrifuging at a low speed of 1000rpm for 10 minutes, and retaining cell precipitates; s3, red blood cell lysis: adding a red blood cell lysis solution, placing at 4 DEG C for 3-5 minutes, centrifuging, and washing and purifying with PBS (Phosphate Buffer Solution); s4, cell culture: resuspending the cells in a culture medium containing an endothelial growth factor replenishing liquid, culturing at 37 DEG C in 5% CO2, and changing the liquid for 24 hours to remove non-adherent cells; and S5, cell identification: carrying out immunofluorescence staining identification 48-72 hours after cell adherence. The method is simple, convenient and efficient to operate, does not need complex digestion, can enrich high-purity and high-activity lymphatic endothelial cells, provides a stable cell model for related pathogenesis research and drug screening, and has important scientific research and clinical value.

The present invention belongs to the field of biomedical technology, and specifically relates to a new application for piR-hsa-164586 and MYH9, based on the interaction between piR-hsa-164586 and MYH9: piR-hsa-164586 regulates the expression of MYH9 and promotes the metastasis of NSCLC, piR-hsa-164586 and MYH9 can be used as a therapeutic target for NSCLC, and have become a potential therapeutic drug, wherein the MYH9 is obtained by RNA pulldown assay and verified by protein mass spectrometry, molecular docking and RIP assays, the piR-hsa-164586 can positively regulate the expression of MYH9, and verified by performed qRT-PCR, western blotting and tissue immunofluorescence staining assays on the piR-hsa-164586 NC group, piR-hsa-164586 KD group, and piR-hsa-164586 OE group. The interaction between piR-hsa-164586 and MYH9 can up-regulate the expression of MYH9 in NSCLC cell lines, the verification experiment process was confirmed by Transwell experiment after knockdown of piR-hsa-164586 and MYH9 respectively.

The invention relates to the technical field of immunofluorescent staining, in particular to an adipose tissue whole-tissue immunofluorescent staining method based on tabletting treatment. According to the method, tissue acquisition and fixation, tissue tabletting, membrane rupture and sealing, degreasing and autofluorescence removal, fluorescent staining, sheet sealing and imaging are sequentially carried out. According to the invention, a complete tabletting-degreasing-fluorescent staining integrated process is established, a set of systematic process which is standardized, high in repeatability and adaptive to conventional experimental conditions is provided, and tabletting whole tissue staining is realized in adipose tissues for the first time. The core of the invention lies in that the thickness of the tissue is reduced by a tabletting treatment means, and a set of optimized antibody permeation, sealing, degreasing and anti-autofluorescence treatment flow is matched, so that the immunofluorescent staining of the adipose tissue is effectively realized on the premise of not depending on complex tissue transparency and special imaging equipment, and the immunofluorescent staining effect is greatly improved. The method is especially suitable for detecting deep or low-abundance targets such as nerve markers.

This invention relates to a rapid screening method for bone-repairing surface structures, belonging to the field of bone repair materials technology. It aims to solve the technical problems of low efficiency and low individual / group adaptability of traditional screening methods. This invention constructs a titanium dioxide nanotube array with a continuous gradient diameter of 0-200 nm using a four-electrode bipolar electrochemical method. Immunofluorescence staining of key bone repair factors BMP-2, RUNX2, OPN, and OCN, combined with panoramic scanning analysis, is used to normalize the average fluorescence intensity of each of the four factors and then sum them to obtain a comprehensive osteogenic coefficient. Based on this coefficient, the optimal bone-repairing surface structure corresponding to bone marrow mesenchymal stem cells (BMSCs) from different age groups or different pathological conditions (such as osteoporosis, osteoarthritis, etc.) can be precisely located. This invention achieves high-throughput screening covering multiple surface structures in a single experiment, providing an efficient technical path for the precise and personalized design of bone repair materials, and has significant clinical translational value.

The invention discloses an integrated micro-fluidic platform for preclinical evaluation of a radiotherapy nano material and an evaluation method thereof, the platform comprises a G chip and a T chip which are cascaded, the G chip generates uniform matrigel liquid drops for encapsulating tumor cells through a flow focusing orifice, and tumor spheres with uniform size and high activity are formed through solidification and fluid perfusion culture; the T chip anchors a tumor sphere through an array type fixing structure, and forms a concentration gradient of a radiotherapy nanometer material through the regulation and control of a double-fluid channel, thereby achieving the precise pretreatment of the tumor sphere. The platform analyzes the activity and proliferation condition of tumor spheres through fluorescence imaging and immunofluorescence staining, and can quickly and reliably evaluate the curative effect of the radiotherapy nano material. The problems that a traditional 2D cell culture model is low in fidelity and an animal model is high in cost are solved, the method has the advantages of being easy and convenient to operate, low in cost and high in fidelity of simulated in-vivo tumor microenvironment, and a new scheme is provided for preclinical evaluation of radiotherapy nanometer materials.

The invention relates to the technical field of biomedicine, in particular to an automatic analysis and risk assessment system and method for peripheral blood endothelial progenitor cells, and the method comprises the following steps: collecting a peripheral blood sample and clinical data of a subject; the method comprises the following steps: enriching CD34 + cells from a peripheral blood sample based on microfluidic-immunomagnetic bead enrichment, carrying out VEGFR2 and DAPI immunofluorescent staining on the enriched cells, and obtaining a multi-channel fluorescent digital image; analyzing the multi-channel fluorescent digital image, identifying and counting VEGFR < 2 + > and DAPI < + > double-positive cells to obtain an EPC counting result; inputting the EPC counting result and clinical data of the subject into a multi-modal AI risk assessment model to obtain a risk assessment result; the health management suggestion information is formulated based on the risk assessment result, so that the model can have better suitability with the target subject, and the accuracy of risk assessment is improved.