Method and device for inducing bone marrow mesenchymal stem cell differentiation and proliferation

Abstract

The invention discloses a method and a device for inducing bone marrow mesenchymal stem cell differentiation and proliferation, and belongs to the technical field of biological cell engineering neural re-restoration. The method comprises the following steps of: (1) taking two conducting copper wires, fixing the two conducting copper wires on the two ends of a conducting glass conducting surface, and performing sterilization treatment for use after the fixation; (2) taking a fresh isolated humerus, cleaning the fresh isolated humerus, then, sucking bone marrow mesenchymal stem cells into a centrifugal tube, removing liquid supernatant through centrifugation, adding the cells into a culture medium to be uniformly blown and beaten, and then inducing the cells into a culture bottle to be culture for 3 to 5 generations for use; and (3) inducing the cells cultured in the second step onto conducting glass processed by the first step, performing electrical stimulation on the cells after the culture, and obtaining the differentiation result of the cells in the direction towards neurons through the immunofluorescence staining and the gene expression detection. The low-frequency alternating current electricity harmless on organisms and cells is used; a physical physical stimulation method is used for stimulating the stem cells, so that the stem cells can differentiate towards the neurons; the operation of the method is easy; the electric stimulation on be given at any time; and high controllability is realized.

Description

technical field [0001] The invention belongs to the technical field of biological cell engineering nerve re-repair, and in particular relates to a method and a device for inducing neural differentiation and proliferation of bone marrow mesenchymal stem cells. Background technique [0002] Nerve injury and neurodegenerative disease, especially central nervous system disease, can cause apoptosis or death of a large number of neurons in the lesion area, which is considered to be a difficult point in clinical treatment. Various methods have been tried in the past for the treatment of such diseases, but the effects are not satisfactory. [0003] Currently, cell therapy is an important treatment method, and its development has brought new hope for the repair of nerve damage. As an important part of cell therapy, stem cells are increasingly used in the research of nerve injury because of their multi-directional differentiation potential and can be induced to differentiate into ner...

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the main methods to promote the differentiation of stem cells to nerves are chemical induction and neurotrophic factor induction, but these induction methods have certain limitations: 1) The induction solution is not safe and the cytotoxicity is high

The chemical induction method is to add an appropriate amount of β-mercaptoethanol, dimethyl sulfoxide, butylated hydroxyanisole and other substances to the medium to affect the differentiation of stem cells, but most of these reagents have strong cytotoxicity and cause a large number of cell death

2) Difficult to be controlled: neurotrophic factors have short half-lives in the process of inducing differentiation, and it is difficult to maintain long-term activity in vivo, so this induction method has certain limitations

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