141 results about "Bone marrow mesenchymal stem cells" patented technology

The application relates to a cartilage organoid based on a DNA-silk fibroin hybrid hydrogel sustained-release system and a preparation method and application thereof. The DNA-silk fibroin hybrid hydrogel sustained-release system is a hydrogel system after a DNA-silk fibroin hybrid hydrogel sustained-release system premixing solution is printed through a digital light processing system, wherein the DNA-silk fibroin hybrid hydrogel sustained-release system premixing solution contains DNA, silk fibroin, acrylated RGD peptide, acrylated polyethylene glycol NHS ester, glucosamine, TD-198946 and 2,4,6-trimethylbenzoyl lithium phosphate. Compared with the prior art, the glucosamine and TD-198946 introduced in the DNA-silk fibroin hybrid hydrogel sustained-release system can significantly improve the cartilage differentiation efficiency of bone marrow mesenchymal stem cells; and the application can be transplanted to a cartilage defect as a cartilage graft to promote cartilage regeneration and repair.

The application discloses a flavonoid compound in eaglewood as well as a preparation method and application of the flavonoid compound, and relates to the technical field of medicines. The application extracts and separates a new compound from natural eaglewood, and the new compound is used in the preparation of anti-osteoporosis drugs. The compound is detected in vitro to obtain an induction effect of the compound on the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts, so as to affect the formation of osteoclasts. Meanwhile, the compound can promote the bone development of zebra fish through RT-qPCR detection, which indicates that the compound has anti-osteoporosis activity. The separation method of the compound is simple and easy to operate.

This invention discloses a composite microsphere loaded with ZIF-8 nanoparticles and stem cell extracellular vesicles, its preparation method, and its applications. The composite microsphere comprises a hydrogel matrix microsphere, and ZIF-8 nanoparticles and small extracellular vesicles derived from bone marrow mesenchymal stem cells uniformly dispersed and encapsulated within the hydrogel matrix microsphere. This invention integrates ZIF-8 nanoparticles and stem cell-derived sEVs into injectable, photocurable GelMA microspheres, constructing an integrated delivery platform capable of sequential release and multi-stage synergistic effects. This platform successfully integrates and leverages three major functions: immunomodulation, stem cell recruitment, and cartilage-induced differentiation, ultimately achieving high-quality functional healing of the tendon-bone interface at both histological and biomechanical levels, providing a highly promising new strategy for the regeneration and repair of interfacial tissues in the musculoskeletal system.

The invention provides hydrogel, a porous titanium / hydrogel composite implant material and a preparation method of the porous titanium / hydrogel composite implant material. The hydrogel is obtained by photo-crosslinking and curing a hydrogel precursor solution containing acrylated beta-cyclodextrin, acrylated alendronate sodium, gelatin and a chemotactic factor. The hydrogel has a porous network structure, high elastic modulus, high fatigue resistance and excellent biocompatibility, and also has the capability of recruiting bone marrow mesenchymal stem cells (BMSCs) and the capability of promoting osteogenic differentiation of the BMSCs; the porous titanium / hydrogel composite implant material prepared from the hydrogel can effectively promote the growth of new bone tissues, has excellent osseointegration capability, obviously improves the osteogenesis repair effect and the osseointegration effect, provides a new treatment approach for the repair treatment of segmental bone defects, and has important medical significance.

The application provides a preparation method of a high-efficiency piezoelectric coating on a titanium implant surface. The preparation method of the application uses a hydrothermal treatment and an in-situ piezoelectric deposition technology to in-situ synthesize a nano strontium titanate-noble metal piezoelectric coating on the titanium implant surface. The coating can generate a piezoelectric potential and active oxygen through ultrasonic driving to degrade extracellular polymers and kill bacteria. In addition, the piezoelectric coating can also promote the osteogenic differentiation of bone marrow mesenchymal stem cells under piezoelectric stimulation, and finally realize the effects of bioactivity, long-acting drug-resistant bacteria and high-efficiency bone integration in the body. The application can provide a new surface treatment technology and research idea for controlling the drug-resistant bacteria infection on the implant surface, help to break through the limitations of the existing theory and technology in removing bacterial infection on the titanium implant surface, and provide beneficial guidance for the control of bacterial biofilm on other parts and the prevention and treatment of related infectious diseases.

The invention provides application of linariin-6-O-glucoside in preparation of a product for preventing and treating osteoporosis, and relates to the field of biological medicine. According to the invention, dyeing experiments of alkaline phosphatase, alizarin red and tartaric acid resistant acid phosphatase prove that the sesaminin-6-O-glucoside has affinity for FPPS receptors, has the capability of promoting bone marrow mesenchymal stem cells to form bone cells, is superior to that of bisphosphate, and can inhibit the generation of osteoclasts; microCT (micro computed tomography) detection results show that the sesaminin-6-O-glucoside has the effect of relieving the osteoporosis and can promote the formation of bone trabecula. According to the application disclosed by the invention, it is proved for the first time that the sesaminin-6-O-glucoside has the effect of resisting osteoclast formation, and meanwhile, the sesaminin-6-O-glucoside also has a better osteogenesis effect, so that a basis is provided for developing the sesaminin-6-O-glucoside as a potential medicine for treating osteoporosis.

The invention provides application of an FZD4 gene. The FZD4 gene is used for regulating osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells. The FZD4 gene in the chicken bone marrow mesenchymal stem cells is over-expressed and is used for promoting osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; or / and the FZD4 gene in the chicken bone marrow mesenchymal stem cells is knocked down to inhibit osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; the nucleotide sequence of the coding region of the FZD4 gene is as shown in SEQ ID NO. 1. According to the invention, the positive regulation effect of the FZD4 gene in osteogenic differentiation of chicken bone marrow mesenchymal stem cells is determined for the first time, the blank of a bone regulation mechanism of the poultry FZD4 gene is filled, and a new biological preparation target is provided for prevention and treatment of poultry bone diseases; the FZD4 gene can also be used as a molecular marker for breeding chicken varieties with high bone strength.

The invention relates to hydrogel for promoting healing of diabetic wounds as well as a preparation method and application of the hydrogel, and belongs to the technical field of hydrogel dressings. The invention discloses an exosome hydrogel, which is characterized in that exosome is loaded in crosslinked aldehyde hyaluronic acid and carboxymethyl chitosan, the aldehyde hyaluronic acid is grafted with L-arginine, and the exosome is derived from bone marrow mesenchymal stem cells. L-arginine can release nitric oxide under the action of a diabetic wound surface or an inflammation microenvironment to achieve the effects of resisting bacteria and promoting wound surface recovery, and the exosome successfully avoids the risks of cell survival defects, vascular embolism, immunological rejection and the like while retaining powerful bioactive components. The product has excellent antibacterial activity, degradability, tissue adhesion, injectable performance and self-repairing performance, and can be suitable for wounds in different shapes.

The invention discloses a surface-enhanced Raman probe as well as a preparation method and application thereof, and belongs to the technical field of biomedical imaging and stem cell therapy. The preparation method comprises the following steps: coating an ultrathin silver shell layer on the surface of gold nanoparticles to obtain Au-coated Ag particles; 4-sulfydryl phenylboronic acid pinacol ester molecules are modified on the surface of Au (at) Ag to form a Raman report layer, then sulfydryl-polyethylene glycol is introduced to realize biological orthogonal coupling, and the modified SERS probe is obtained through bovine serum albumin modification; jointly incubating the modified SERS probe and the azidation-labeled mouse bone marrow mesenchymal stem cells, so as to form an mmol / LSC-SERS composite probe; the obtained probe has good biocompatibility, stability and anti-interference capability and is suitable for a complex physiological environment, and the preparation method is simple and good in repeatability, is completely carried out in a water phase and does not need an organic solvent.

The invention discloses a siRNA molecule for interfering sheep TBXT gene expression in primary culture of sheep bone marrow mesenchymal stem cells and application of the siRNA molecule, and relates to the technical field of molecules. According to the invention, a method for in-vitro targeting sheep TBXT gene interference siRNA transfected cells is established by synthesizing a specific targeting combination sheep TBXT gene small fragment sequence, and the mRNA expression level of the TBXT gene of sheep bone marrow mesenchymal stem cells can be effectively interfered. A further research proves that the proliferation efficiency of the sheep bone marrow mesenchymal stem cells is obviously reduced after the TBXT gene is interfered. The TBXT gene is helpful for researching the biological function of the TBXT gene participating in embryonic caudal vertebra development regulation, and has good application value for researching the molecular mechanism of the sheep tail type character.

The application discloses application of microRNA-32-5p in preparation of a medicine for preventing and treating osteoporosis, and a medicine composition taking microRNA-32-5p as an active ingredient. It is proved through experiments that knocking out the microRNA-32-5p gene significantly aggravates osteoporosis induced by estrogen deficiency; the microRNA-32-5p plays a bone protection role by promoting osteogenic differentiation of bone marrow mesenchymal stem cells. Therefore, the microRNA-32-5p can be applied to prevention and treatment of osteoporosis as a microRNA medicine.

The invention belongs to the field of biological medicine, and relates to a curcumin-loaded bone marrow mesenchymal stem cell source exosome which is obtained by the following steps: step 1, culturing BMSCs (bone marrow mesenchymal stem cells): simultaneously adding 10% of fetal calf serum and 1% of myclostreptomycin into an MEM culture medium, culturing at 37 DEG C under 5% CO2, and counting and subculturing when the cell density reaches 90%; 2, dissolving Cur in a DMSO solution, preparing a mother solution with the culture medium, and filtering and diluting to 30-50 [mu] g / mL; adding into BMSCs (bone marrow mesenchymal stem cells) with the cell density of 85-100%, and culturing for 12-36 hours; collecting supernate, and carrying out gradient centrifugation to obtain the mesenchymal stem cell source exosome loaded with curcumin. The invention provides a novel administration route which depends on the self function of the mesenchymal stem cell source exosome and the self function of the curcumin at the same time.

The invention belongs to the technical field of biological medicine, and particularly relates to a small-molecule exosome-loaded composition based on bone marrow mesenchymal stem cells and application of the small-molecule exosome-loaded composition. The invention discloses the treatment effect of squalene on osteoarthritis for the first time, and further prepares the squalene-loaded bone marrow mesenchymal stem cell exosome. An in-vitro cell experiment and a model animal experiment prove that the composition has a synergistic treatment effect on osteoarthritis. In-vitro tests prove that the squalene-loaded mesenchymal stem cell exosome can improve the survival rate of inflammatory cartilage cells, inhibit release of proinflammatory factors and restore expression of repair factors. Model animal experiments prove that the squalene-loaded mesenchymal stem cell exosome has the effects of resisting inflammation, easing pain and protecting cartilage in the treatment of osteoarthritis.

This invention belongs to the field of biomedical technology, specifically relating to the application of mesenchymal stem cells in the preparation of anti-aging drugs. The mesenchymal stem cells are human adipose-derived mesenchymal stem cells (hAMSCs) or mouse bone marrow mesenchymal stem cells (BM-MSCs). This invention utilizes mesenchymal stem cells to regulate senescent stromal cells and the spleen's ECM, improving the thinning stromal state in the tissue microenvironment through multiple targets. Specifically, it improves the aging-related secretory phenotype of stromal cells, enhances mitochondrial activity, strengthens their proliferation capacity, and delays the reduction of collagen secretion. This, in turn, delays the disordered distribution and functional decline of immune cells caused by aging and enhances tissue regeneration capacity, indirectly affecting various other cells in the tissue microenvironment, thus exhibiting an antagonistic effect against systemic organ aging.

The invention discloses a bone targeting forsythiaside A loaded exosome and a preparation method and application thereof, and relates to the technical field of biomedicine.The preparation method comprises the following steps that bone marrow mesenchymal stem cells are separated and cultured, and GLG1 is over-expressed through a molecular cloning technology to obtain GLG1-BMSCs; the exosome GLG1-Exos of the GLG1-BMSCs is collected through an ultracentrifugation method; and loading forsythiaside A into the exosome through an ultrasonic method and an extrusion method to obtain the GLG1-Exos-FTA. The traditional Chinese medicine active ingredient forsythiaside A is loaded, and forsythiaside A is delivered to bone marrow in a targeted manner, so that the effect of preventing and improving diabetic osteoporosis is achieved; due to the targeting effect, the bioavailability of forsythiaside A can be enhanced, and meanwhile, the curative effect of resisting diabetic osteoporosis is improved. Experiments show that the loading rate of forsythiaside A of the GLG1-Exos-FTA reaches 36.9%, the particle size distribution peak value is 122.4 nm, and the GLG1-Exos-FTA has typical exosome characteristics and bone targeting, and can significantly improve the bone microstructure of diabetic osteoporosis mice.

The invention discloses a rehmannia neutral homogeneous polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine preparations. The polysaccharide mainly comprises galactose, glucose and fructose, the molar ratio of the galactose to the glucose to the fructose is 2.72: 1.17: 1.11, and the weight-average molecular weight of the polysaccharide is 2152 Da. The rehmannia neutral polysaccharide can play a role in treating osteoporosis by increasing bone mineralization amount and bone mineral density; the proliferation and directional osteogenic differentiation of the bone marrow mesenchymal stem cells BMSCs can be promoted, and the obvious application effect is achieved in the aspect of preparing the bone defect treatment medicine through the stem cells. The rehmannia neutral homogeneous polysaccharide shows a huge application prospect in the aspects of preparation of medicines for treating osteoporosis and stem cell treatment (especially bone regeneration treatment based on mesenchymal stem cells). A new candidate substance and a research direction are provided for developing an efficient and safe bone metabolism disease treatment strategy derived from traditional Chinese medicines.

The invention provides a composite growth factor composition capable of promoting proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells, a method and application. The invention provides the composite growth factor composition by simulating a natural secretion mode of a decalcified bone matrix of the mandible of an SD (Sprague Dawley) rat on the seventh day, the composite growth factor composition comprises BMP-2 (400-600 pg / ml), TGF-beta1 (5-7 ng / ml) and IGF-1 (150-250 ng / ml), and the optimal concentrations are 500 pg / ml, 6 ng / ml and 200 ng / ml respectively. The composition is remarkably superior to a single factor and a high-dosage combination in the aspects of promoting BMSCs proliferation, improving the activity of alkaline phosphatase (ALP) and forming mineralized nodules, meanwhile, the use dosage of growth factors is remarkably reduced, and the risk of side effects such as heterotopic ossification is reduced. The composite growth factor composition has good biocompatibility and safety, and is suitable for the fields of bone tissue engineering, bone defect repair and related regenerative medicine.

The invention provides a composition for delaying cell senescence as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: loading tea polyphenol on a dog bone marrow mesenchymal stem cell exosome, and modifying the surface of the dog bone marrow mesenchymal stem cell exosome with arginine nonamer to prepare a modified tea polyphenol-loaded exosome; the preparation method comprises the following steps of: modifying cholesterol by using arginine nonamer, and preparing lipidosome by using the cholesterol modified by the arginine nonamer, DSPE-PEG2000, phospholipid embedded resveratrol and epigallotein gallate; polysaccharide is extracted from rhizoma polygonati, fructus lycii and ginseng, and rhizoma polygonati / fructus lycii / ginseng polysaccharide is prepared; the preparation method comprises the following steps: mixing an acellular dermal matrix with polygonatum sibiricum / medlar / ginseng polysaccharide, adding lipidosome and a modified tea polyphenol-loaded exosome, co-incubating, and freeze-drying to prepare the composition for delaying cell aging, the composition has a better drug embedding effect, can improve the stability and transdermal absorption effect of the drug, has better antioxidant, antibacterial and anti-inflammatory effects, and can be used for preparing the medicine for delaying cell aging. The effect of delaying skin cell senescence can be better achieved through multiple ways, and safety and degradability are achieved.

The invention relates to a living plant cell-animal cell composite scaffold as well as a preparation method and application thereof. The living plant cell-animal cell composite scaffold comprises a 3D printing hydrogel scaffold used as a muscle repair carrier and a 3D printing biological ceramic scaffold used as a bone repair carrier, wherein the 3D printing hydrogel scaffold and the 3D printing biological ceramic scaffold are assembled by simulating a muscle-skeleton structure; the hydrogel scaffold comprises a polymer matrix component, and functional plant cell components, namely diatom cells and mammalian cell components, namely muscle stem cells, which are distributed in the polymer matrix component, the biological ceramic scaffold comprises a biological ceramic scaffold matrix component and mammalian cell component bone marrow mesenchymal stem cells inoculated and loaded on the biological ceramic scaffold matrix component.

The invention discloses application of a mesenchymal stem cell combined platelet in preparation of a preparation for treating ankle arthritis, the mesenchymal stem cell combined platelet (platelet-rich plasma) is adopted to treat the ankle arthritis, the operation is simple, the side effect is small, the treatment effect is remarkable, the treatment period is short, and the application is safe and reliable. The platelet-rich plasma is rich in growth factors, can promote the regeneration of damaged tissue vessels, increase blood supply and nutrient substances, enhance the biological activity and migration ability of cartilage cells, accelerate the healing of damaged tissues around ankle joints, and effectively promote the growth and reproduction and osteogenic differentiation of bone marrow mesenchymal stem cells (BM-SCs). The method has the advantages that the cartilage differentiation of the mesenchymal stem cells at the joints is promoted, the cell repair and regeneration of the affected parts of the joints are realized, the functional recovery of the joints is promoted, and the ankle arthritis is treated more efficiently.

The invention provides a preparation method of dynamic connection bone extracellular matrix simulation hydrogel and application of the dynamic connection bone extracellular matrix simulation hydrogel in bone defect repair, and belongs to the technical field of medical hydrogel. L-cysteine ethyl ester functionalized hyaluronic acid and allicin are prepared to form a thiol-disulfide bond dynamic cross-linked network; a sacrificial pre-vascularization template is constructed by combining a collagen thread loaded with a vascular endothelial growth factor, a vascularization guide microchannel is formed by utilizing the enzymolysis characteristic of collagen, the polarization of macrophages is regulated and controlled by hyaluronic acid to inhibit inflammatory response, and the adhesion and osteogenic differentiation of mesenchymal stem cells are promoted by virtue of a functional surface, so that the osteogenic differentiation of the mesenchymal stem cells is promoted. The synergistic effect of vascularization, anti-inflammation and osseointegration is achieved, finally, the dynamic connection bone extracellular matrix simulation hydrogel with a porous interconnection structure and biological activity is formed, and the technical problems that a traditional bone defect repair material is insufficient in vascularization, inflammation interference exists, and osseointegration is poor are solved.

The invention discloses an alpha-ketoglutaric acid functionalized hydrogel and application thereof in maxillofacial bone defect repair, and belongs to the technical field of biomedical materials. The hydrogel is constructed by crosslinking four-arm polyethylene glycol-sulfydryl and alpha-ketoglutaric acid through a Fe < 3 + > coordination bond. The hydrogel prepared by the invention has good mechanical properties, self-healing ability and inflammatory microenvironment responsive drug release characteristics, and can synergistically promote osteogenic differentiation of bone marrow mesenchymal stem cells, induce M2 polarization of macrophages and remove active oxygen, thereby optimizing a bone regeneration microenvironment. In-vivo experiments prove that the hydrogel can significantly promote the formation of new bones of a mouse jaw defect model. The invention provides a novel and efficient material solution for regeneration and repair of maxillofacial bone defects.

This invention discloses a microgel-assembled bilayer scaffold for integrated osteochondral repair and its preparation method. The preparation method includes the following steps: (1) synthesizing gelatin modified with carbon-carbon double bonds and dopamine; (2) preparing modified gelatin composite microgels loaded with bioactive glass and chondroitin sulfate respectively; (3) preparing hyaluronic acid modified with carbon-carbon double bonds and phenylboronic acid; (4) suspending the modified gelatin composite microgels loaded with bioactive glass and the modified gelatin composite microgels loaded with chondroitin sulfate in modified hyaluronic acid solutions respectively, and loading them into two different syringes; (5) injecting the two microgel suspensions sequentially into a mold, and constructing the microgel-assembled bilayer scaffold in situ under ultraviolet light. The scaffold of this invention has an open porous structure and good cell compatibility, which can promote the bidirectional differentiation of bone marrow mesenchymal stem cells into chondrogenic and osteogenic cells, and can be used for integrated regeneration and repair of osteochondral tissue defects.

The application discloses a customizable biphasic hydrogel scaffold, which comprises a transparent cartilage layer and a subchondral bone layer, the transparent cartilage layer comprises methacrylated type I recombinant collagen and bone marrow mesenchymal stem cells, and the subchondral bone layer comprises methacrylated type I recombinant collagen and resveratrol-loaded bioactive glass. The application further provides a preparation method and application of the biphasic hydrogel scaffold. The biphasic hydrogel scaffold has the advantages of customization, antioxidation, anti-inflammation, high biocompatibility, promotion of wound healing and bone repair and the like.

The invention relates to a fixing device for loading low-intensity pulsed ultrasound by taking water as a medium and application of the fixing device in promoting osteogenic differentiation of mesenchymal stem cells. According to the device, a double-base type probe fixing structure consisting of a main body fixing base and an accessory fixing base is arranged in a main body structure; and a culture vessel fixing structure on the inner wall of the main body structure is matched, so that the problems that a probe is easy to shift, the heights of culture vessels are inconsistent and the like in the prior art are effectively solved, and a standardized and high-repeatability loading environment is provided for an LIPUS in-vitro experiment. The device is further based on the device, water is used as an ultrasonic conduction medium to be applied to osteogenic differentiation induction of bone marrow mesenchymal stem cells (BMSCs), the efficient and stable osteogenic stimulation effect can be achieved, and the core defects that in the prior art, a probe makes direct contact with a culture vessel, consequently, cells fall off and are curled, morphological damage is caused, and sound field distribution is uneven are overcome; and meanwhile, the stability and repeatability of an experimental result are remarkably improved.

The invention discloses application of keratin in preparation of a bone repair material, and relates to the technical field of biomedical engineering. The preparation method comprises the following steps: extracting bone marrow mesenchymal stem cells, and carrying out osteogenic induction culture under the intervention of keratin with the concentration of 100-800 [mu] g / ml; the culture conditions of the BMSCs are as follows: in a constant-temperature incubator with the temperature of 37 DEG C and 5% CO2, a culture medium contains a precooled complete culture medium; the first liquid changing time of the primary culture of the BMSCs is 48-72 hours, then the liquid is changed once every 2-3 days, and passage is carried out when the cell fusion degree reaches 80%-90%. Keratin is adopted as a natural biological material, has good biocompatibility and biodegradability and can well interact with cells, and immunological rejection is reduced.

The invention belongs to the technical field of cell culture, and particularly relates to a bone marrow mesenchymal stem cell culture medium and a preparation method and application thereof. The mesenchymal stem cell culture medium comprises a basic culture medium, and betaine, glycyrrhizin, insulin, EGF (Epidermal Growth Factor), L-propyl dipeptide and naloxicol which are added into the basic culture medium. The bone marrow mesenchymal stem cell culture medium can effectively improve the multiplication capacity of bone marrow mesenchymal stem cells, delay cell senescence and improve the osteogenic differentiation capacity of the bone marrow mesenchymal stem cells, fetal calf serum is not added, the risk of introducing heterologous serum to carry viruses is avoided, and the safety of clinical application is improved.

This invention belongs to the field of guided bone regeneration materials technology, specifically relating to an arginine carbon dot photoinitiated hydrogel prepolymer, its preparation method, and its application. The arginine carbon dot photoinitiated hydrogel prepolymer of this invention, when used for alveolar bone regeneration, inhibits the proliferation / migration of gingival fibroblasts (hGFs) while simultaneously inducing and promoting the proliferation / migration and osteogenic differentiation of bone marrow mesenchymal stem cells (hBMSCs), thus creating a "reverse-response" cell-selective regulatory effect.

The invention discloses a stem cell-loaded microcapsule, a preparation method and application, the stem cell-loaded microcapsule is composed of hydrogel and stem cells, and the stem cells are wrapped in the stem cell-loaded microcapsule; the stem cells are selected from at least one of umbilical cord mesenchymal stem cells, bone marrow mesenchymal stem cells, adipose-derived stem cells, embryonic stem cells and induced pluripotent stem cells; the number of living cells in the stem cell-loaded microcapsule is 1-10; the particle size of the stem cell-loaded microcapsule is 30-200m, and the dispersion coefficient of particle size distribution is 0.01-20%. According to the preparation method, stem cells and a biological material are mixed to prepare a prepolymerization solution, the biological material provides immune protection for the cells, the in-vivo retention time of the cells is prolonged, and the problem that at present, the stem cells are rapidly removed by an immune system after being transplanted into the body is solved. The capsule can be used as bio-ink to be assembled into a bionic stent through 3D printing or directly injected to an injured part, and has a wide application prospect.

The invention discloses an application of loperamide in preparation of a medicine for treating osteoporosis, and belongs to the field of development of new application of medicines. Loperamide has the effects of inhibiting aging, increasing osteogenic differentiation of bone marrow mesenchymal stem cells and preventing osteoporosis of aged and postmenopausal mice; meanwhile, at the cellular level, loperamide can reduce expression of senescence factors in mouse mesenchymal stem cells, promote senescence cell apoptosis and inhibit cell senescence; and a new choice is provided for the treatment of senescence-related osteoporosis drugs.