390 results about "Bone marrow mesenchymal stem cells" patented technology

The invention relates to a bone organ constructed based on oxygen-releasing immune hydrogel microspheres and a preparation method and application thereof. An oxygen release material, macrophages and bone marrow mesenchymal stem cells are loaded in the oxygen release immune hydrogel microspheres. And adding the oxygen-releasing immune hydrogel microspheres into a culture medium for culturing to obtain the bone organ constructed based on the oxygen-releasing immune hydrogel microspheres. The oxygen release material (CaO2-HAp) is entrapped in the hydrogel microspheres, so that continuous oxygen supply can be provided in a defect area, the low-oxygen environment is improved, the activity of osteoblasts is improved, osteogenic differentiation is promoted, macrophages are entrapped by the microspheres, the immune microenvironment is regulated and controlled, the release of proinflammatory cytokines is reduced, the osteogenic efficiency is improved, and bone defect repair is accelerated. The micro-fluidic technology is utilized to accurately control the size and shape of the microspheres, so that the microspheres can adapt to irregular bone defects, bone organs are formed through long-term in-vitro culture, and the biological activity and adaptability of the bone repair material are improved.

The invention relates to bionic matrix hydrogel for constructing bone organs and a preparation method of the bionic matrix hydrogel. The bionic matrix hydrogel is prepared from a bone acellular extracellular matrix, a salmon DNA (Deoxyribose Nucleic Acid) network and a calcium phosphate oligomer under the combination of photo-crosslinking and self-assembly physical crosslinking. The acellular extracellular matrix in the bionic matrix hydrogel can simulate organic components in natural bones, the calcium phosphate oligomer can simulate inorganic components in the natural bones, and sufficient phosphate ions can be provided for biomineralization due to introduction of a DNA network. The biomimetic matrix hydrogel can replace Matrigel to act in bone organoid culture, provide a living environment similar to bone tissue for three-dimensional culture cells, enhance substance exchange and signal transduction between cells, promote proliferation, adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells, and further accelerate the process of biomineralization. The invention provides an optional new material for preparing the functional bone organ.

The present invention relates to the field of biomedical materials. Particularly provided is a DNA-silk fibroin composite hydrogel microsphere. The DNA-silk fibroin composite hydrogel microsphere is prepared from DNA and silk fibroin on the basis of the micro-fluidic technology by means of combination of self-assembly physical crosslinking and photo-crosslinking. The introduction of a silk fibroin network into the composite hydrogel microsphere can provide mechanical performance for a DNA hydrogel network and reduce the production cost. RGD can remarkably improve the cell loading capability of the microsphere. The hydrogel microsphere can simulate a microenvironment of cartilage tissue by means of three-dimensional loading of high-density cells, so as to enhance substance exchange and signaling between cells, and more effectively promote the proliferation and adhesion of bone marrow mesenchymal stem cells and regulating the chondrogenic differentiation, thus promoting cartilage regeneration and repair, and providing an available material for preparing medicaments or medical instruments for cartilage defect repair.

The invention provides a bone organ model as well as a construction method and application thereof, and the method comprises the following steps: arranging a biological material with a micropore array in a cell culture plate to obtain a bone organ chip; suspending the initial stem cells in a culture medium, and promoting the cells to form a spherical structure through cell aggregation or by using a non-adhesive culture surface, so as to obtain cell spheres; and culturing the cell spheres in the bone organ chip by adopting the culture medium for constructing the bone organ model to obtain the bone organ model. The skeletal organ is successfully constructed through in-vitro self-organized 3D co-culture initial stem cells including embryonic stem cells, induced pluripotent stem cells, bone marrow mesenchymal stem cells, osteoblasts, bone cells, all levels of osteogenic precursor cells, osteogenic-like cells and MC3T3-E1 cells, and the skeletal organ can become an important tool for researching and treating bone related diseases.

The invention relates to a cartilage organ constructed on the basis of a DNA-silk fibroin hybrid hydrogel sustained-release system as well as a preparation method and application of the cartilage organ. The DNA-silk fibroin hybrid hydrogel sustained-release system is a hydrogel system formed by printing a DNA-silk fibroin hybrid hydrogel sustained-release system premix solution through a digital light processing system, and the DNA-silk fibroin hybrid hydrogel sustained-release system premix solution contains DNA, silk fibroin, acryloylated RGD peptide, acryloylated polyethylene glycol NHS ester, glucosamine, TD-198946 and 2, 4, 6-trimethyl-1, 3-pentanediol. The invention discloses a lithium 2, 6-trimethylbenzoyl phosphate. Compared with the prior art, the glucosamine and the TD-198946 introduced into the DNA-silk fibroin hybrid hydrogel sustained-release system disclosed by the invention can be used for remarkably improving and promoting the cartilage differentiation efficiency of the bone marrow mesenchymal stem cells; the cartilage graft can be transplanted to a cartilage defect position as a cartilage graft to promote cartilage regeneration and repair.

Disclosed are methods of treating perianal fistula a subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention relates to an in-situ osteochondral regeneration and repair scaffold and a preparation method thereof, the in-situ osteochondral regeneration and repair scaffold prepared by the method comprises a cartilage layer, a connecting layer and a subchondral bone layer, the porosity of the cartilage layer is not less than 85%, the Young modulus is 6-13KPa, and the aperture is 130-140 [mu] m; the porosity of the connecting layer is not lower than 95%, the Young modulus is 4-19 KPa, and the aperture is 50-120 [mu] m; the porosity of the subchondral bone layer is not lower than 95%, the Young modulus is 4-19 KPa, and the aperture is 300-400 microns. The in-situ osteochondral regeneration and repair scaffold prepared by the method has good biocompatibility, is beneficial to attachment, migration and proliferation of cartilage cells and bone marrow mesenchymal stem cells, does not cause immunological rejection, provides a better scaffold environment for repair after osteochondral implantation, and has a good application prospect in the field of in-situ osteochondral regeneration and repair, and experiments prove that the in-situ osteochondral regeneration and repair scaffold has a good application prospect in the field of in-situ osteochondral regeneration and repair. The in-situ osteochondral regeneration and repair scaffold does not affect cell migration; animal experiments prove that the in-situ osteochondral regeneration and repair scaffold has a good repair effect on rat osteochondral defects.

The invention discloses composite gel for osteochondral repair as well as a preparation method and application of the composite gel. Main raw materials of the composite gel are regenerated silk fibroin (RSF) and recombinant humanized III-type collagen (rhCol-III), and auxiliary raw materials of the composite gel are sodium hyaluronate (HA), fibrinogen and thrombin. The preparation method comprises the following steps: firstly, mixing an RSF solution with an rhCol-III solution, obtaining RSF-rhCol particles through a freeze drying technology and mechanical pulverization, dispersing the particles in an HA solution to obtain composite gel, mixing the gel with a thrombin solution, catalyzing fibrinogen to be split and converted into fibrin by thrombin, and performing freeze drying to obtain the rhCol-RhCol-RhCol-RhCol-RhCol-RhCol-RhCol. And crosslinking the fibrous protein monomer to obtain the injectable composite gel preparation capable of immediately gelling. The prepared RSF-rhCol composite gel can adapt to a defect area, has a certain adhesion effect, has excellent biocompatibility, promotes migration of bone marrow mesenchymal stem cells, induces cell differentiation and promotes cartilage tissue regeneration.

The invention relates to the technical field of biomedical materials, and particularly discloses a preparation method of bionic hydrogel capable of bidirectionally inducing BMSCs (bone marrow mesenchymal stem cells) to promote epiphyseal plate repair through regulation and control mechanics, which comprises the following steps: step 1, mixing 10wt% of methacrylated gelatin, 10wt% of methacrylated decellularized cartilage matrix and 0.5 wt% of phenyl (2, 4, 6-trimethyl-1, 3-pentanediol) according to a ratio of the methacrylated gelatin to the methacrylated The preparation method comprises the following steps: respectively preparing methacrylated gelatin and methacrylated acellular cartilage matrix pre-polymerization solutions according to the concentration of (1, 2, 6-trimethylbenzoyl) lithium phosphate, and carrying out 37 DEG C constant-temperature dark water bath until the methacrylated gelatin and methacrylated acellular cartilage matrix pre-polymerization solutions are completely dissolved for later use; and 2, mixing the 10 wt% methacrylated decellularized cartilage matrix prepolymer solution and the 10 wt% methacrylated gelatin prepolymer solution according to a ratio of 3: 7 to obtain a methacrylated gelatin / methacrylated decellularized cartilage matrix prepolymer solution, carrying out cross-linking under blue light with a wavelength of 365 nm for 5-20 s, and carrying out drying to obtain the methacrylated gelatin / methacrylated decellularized cartilage matrix. The methylacryloylated gelatin / methylacryloylated acellular cartilage matrix composite hydrogel is prepared from the methylacryloylated gelatin and the methylacryloylated acellular cartilage matrix.

The invention relates to a method for constructing an injectable bone organ to realize rapid bone regeneration. The method comprises the following steps: 1, obtaining a water-soluble periosteum acellular matrix by adopting an acellular technology; secondly, compound bone salt is prepared according to the proportion of natural bone salt components, the water-soluble periosteum acellular matrix and the compound bone salt are mixed according to the proportion of natural bone organic and inorganic components to form a bionic matrix component, and then the bionic matrix component is added into gelatin to construct a completely bionic osteogenesis microenvironment; and 3, constructing a bone regeneration material to form a bone organ, firstly preparing a granular gelatin scaffold with a full-bionic osteogenesis microenvironment, then inoculating the bone marrow mesenchymal stem cells, finally performing in-vitro culture, and regulating the differentiation and secretion of the bone marrow mesenchymal stem cells by virtue of the osteogenesis microenvironment of the scaffold to finally form the bone organ.

The invention belongs to the technical field of biomedical materials, and particularly relates to a gradient bionic wood-based collagen bone-cartilage repair scaffold and a preparation method and application thereof.The scaffold comprises a surface layer, a middle layer and a bottom layer, the surface layer is composed of collagen, the middle layer is composed of collagen and nano-hydroxyapatite, and the bottom layer is composed of collagen and nano-hydroxyapatite; the bottom layer is composed of wood, collagen and nano-hydroxyapatite, the scaffold is obtained by stacking the surface layer, the middle layer and the bottom layer and then conducting freeze-drying, the structure of the scaffold is similar to that of natural osteochondral cartilage, the surface layer is of a porous structure communicated with one another, and the bottom layer is of a directionally-arranged pore channel structure; the main composition of a natural bone cartilage tissue can be highly simulated, and the gradient is presented; the bone marrow mesenchymal stem cell prepared by the method has the advantages of high mechanical performance, gradient enhancement from the surface layer to the bottom layer, excellent biocompatibility and biological activity, capability of remarkably promoting cell proliferation and gradient differentiation of bone marrow mesenchymal stem cells, and application to osteochondral tissue regeneration and clinical treatment of osteoarthritis.

Disclosed are methods of improving perioral and face fat graft retention in a subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention belongs to the technical field of biological medicine, and discloses a high-efficiency vascular endothelial cell targeting vesicle system, which is prepared from hemangioma stem cell derived exosome HemSCs-Exos and bone marrow mesenchymal stem cell derived apoptotic small body BMSCs-ABs, and is characterized in that the endothelial cell targeting vesicle system is prepared from the hemangioma stem cell derived exosome HemSCs-Exos and the bone marrow mesenchymal stem cell derived apoptotic small body BMSCs-ABs; meanwhile, the invention further discloses a preparation method of the vesicle system and application of the vesicle system in promoting proliferation and migration of endothelial cells. Different from existing targeting modification, the ingenious targeting relationship between the BMSCs-ABs and the endothelial cells belongs to non-special modification combination, and is simple and efficient; the fusion between the double EVs has the characteristic of realizing '1 + 1gt, 2', not only retains the treatment components of the HemSCs-Exos and the BMSCs-ABs, but also the fused eHEs has remarkable cell specificity distribution, and the fusion mode is simple and stable.

The invention relates to a two-phase hydrogel system based on space-time gradient regulation and control as well as a preparation method and application of the two-phase hydrogel system. The biphase hydrogel system can also be expressed as a GelMA composite hydrogel scaffold of a porous structure, the whole biphase hydrogel system is of a core-shell structure, the inner layer core mineralizes SilMA hydrogel microspheres, the porous GelMA hydrogel scaffold is used as an outer layer, and the angiogenesis promoting factor dimethyl oxalaminoacetic acid, the human umbilical vein endothelial cells and the bone marrow mesenchymal stem cells are loaded on the outer layer. Wherein the inner-layer core mineralized SilMA hydrogel microspheres realize continuous release of osteogenesis signals by slowly releasing nHAp, and the outer-layer porous GelMA hydrogel stent releases DMOG by rapid degradation to realize early angiogenesis, so that space-time coupling construction of blood vessels and bone tissues is realized. Through differential degradation characteristics of inner and outer layers of hydrogel, precise time sequence cooperative regulation and control of angiogenesis and bone tissue remodeling can be realized, and the bionic property and function integration capability of a vascularized bone organ structure can be improved, so that the clinical transformation potential of the vascularized bone organ structure is improved.

The invention relates to a recombinant collagen bionic artificial bone and a preparation method and application thereof.The recombinant collagen is subjected to cross-linking modification, the molecular weight and viscosity of the recombinant collagen are increased, a fiber network structure is formed, then the calcium-phosphorus ratio of hydroxyapatite is simulated through soluble salts of calcium chloride and sodium dihydrogen phosphate, and the recombinant collagen bionic artificial bone is obtained. The biomimetic artificial bone can replace insoluble hydroxyapatite to mineralize the recombinant collagen, so that the utilization rate of the collagen is improved, and the obtained biomimetic artificial bone can exert a better osteogenesis effect. The obtained bionic artificial bone is high in biological safety, has high bionic characteristics, good biocompatibility and mechanical properties matched with human bones, can induce bone marrow mesenchymal stem cell osteogenic differentiation, and has great potential in the field of bone defect repair.

The invention discloses a method for promoting diabetic fracture healing by using bone marrow mesenchymal stem cell exosomes, which comprises the following steps: separating MSCs (mesenchymal stem cells) from the bone marrow of a diabetic patient, culturing and extracting the exosomes; the method comprises the following steps: extracting an exosome from a bone fracture part, performing quantitative analysis on the extracted exosome, injecting the exosome into the bone fracture part in a manner of intravenous injection, local injection or minimally invasive injection, continuously treating, promoting proliferation and differentiation of osteoblasts by miRNA or mRNA in the exosome through targeting cells, enhancing angiogenesis, and further accelerating fracture healing. According to the exosome, proliferation and differentiation of osteoblasts are promoted through miRNA or mRNA in the exosome, the synthesis capacity of bone matrixes of the osteoblasts is enhanced, so that fracture healing is accelerated, meanwhile, the exosome provides necessary blood supply for fracture healing by adjusting vascular endothelial cells and promoting angiogenesis, and the repair capacity of fracture parts is effectively improved.

The invention relates to the technical field of biological medicine, in particular to a chronic disease treatment composition based on mesenchymal stem cells and a preparation method thereof, and the chronic disease treatment composition comprises the mesenchymal stem cells; the mesenchymal stem cells are adipose mesenchymal stem cells, dental pulp mesenchymal stem cells and bone marrow mesenchymal stem cells. According to the invention, the culture medium of the adipose-derived mesenchymal stem cells is improved, and the prepared adipose-derived mesenchymal stem cells have higher proliferation activity. Meanwhile, through experimental tests, the adipose-derived stem cells can inhibit proliferation of the prostatic hyperplasia epithelium BPH-1 cells and remarkably promote apoptosis of the prostatic hyperplasia epithelium BPH-1 cells, a new direction is provided for treatment of prostatic hyperplasia, and the adipose-derived stem cells have huge application value.

The invention provides application of a deacetylase inhibitor or a derivative thereof and application of a pharmaceutical composition, and belongs to the technical field of biology. The application of the deacetylase inhibitor or the pharmaceutically acceptable derivative thereof comprises the application of the deacetylase inhibitor for preparing a medicine for treating osteoporosis; wherein the deacetylase inhibitor is used for inducing histone acetylation in a cell nucleus. By inducing acetylation of histone in a cell nucleus, the differentiation direction of the bone marrow mesenchymal stem cells is accurately and efficiently controlled, and a treatment scheme for osteoporosis related diseases is developed.

The invention discloses a silk fibroin-hyaluronic acid temperature-sensitive bioactive hydrogel scaffold, a preparation method thereof and application of the silk fibroin-hyaluronic acid temperature-sensitive bioactive hydrogel scaffold in TBI treatment, hyaluronic acid, silk fibroin and N-isopropylacrylamide are used as raw materials to construct temperature-sensitive hydrogel, and bone marrow mesenchymal stem cells (BMSCs) and paeoniflorin (PF) are loaded to form a treatment unit. Hyaluronic acid is one of components of an extracellular matrix (ECM), has high affinity to cells, and can promote proliferation of stem cells. Silk fibroin has high biocompatibility and biodegradability and good mechanical strength and ductility / toughness, and can promote tissue repair and stem cell attachment and proliferation. N-isopropylacrylamide endows the hydrogel with the characteristic of in-situ temperature-sensitive gelling. Therefore, the bioactive hydrogel stent has the advantages of components, combines the BMSCs and the PF, overcomes the obstacle that the medicine is limited by BBB to be transferred into the brain, and is implanted into a brain injury part after a TBI surgical operation to be used for repairing and treating nerve injury.

The invention relates to a time sequence degradable orthopedic implant material which comprises a base material and a biodegradable coating formed on the surface of the base material, the surface of the base material is provided with a nanocone array microstructure, the biodegradable coating takes a biodegradable polymer as a matrix and loads a metal element for promoting bone repair, and the metal element is a metal element for promoting bone repair. And the surface of the biodegradable coating is also connected with an anti-inflammatory active factor. According to the invention, grafted anti-inflammatory active factors are matched with the biodegradable coating loaded with metal elements for promoting bone repair, and the sequential active regulation and control of the biological effect of the micro-morphology on the surface of the base material cope with effector cells in different repair stages; the time sequence regulation and control on the macrophage inflammation phenotype and bone marrow mesenchymal stem cell osteogenic differentiation in different stages of bone repair are realized, so that each stage of bone tissue repair is matched, and bone repair and osseointegration are promoted.

The invention provides a composite hydrogel based on chitosan and an acellular matrix. The composite hydrogel comprises methacrylic acid chitosan and a methacrylic acid modified acellular matrix, the prepared composite hydrogel has good injectability, good degradability and relatively high mechanical properties, and can regulate the aggregation of the bone marrow mesenchymal stem cells (BMSCs), and the bone marrow mesenchymal stem cell composite hydrogel has a remarkable effect of repairing transparent cartilage by transplanting a cartilage defect model.

The invention relates to the technical field of bone marrow mesenchymal stem cell proliferation, and particularly discloses an in-vitro proliferation method of bone marrow mesenchymal stem cells, which comprises the following steps: S1, preparing rat bone marrow mesenchymal stem cells, performing constant-temperature culture on the rat bone marrow mesenchymal stem cells under the condition of 5% CO2 at 36-37 DEG C, and then performing passage; s2, taking third-generation rat bone marrow mesenchymal stem cells, adjusting the cell concentration to 1 * 10 < 7 > cells / L, culturing in the basic culture medium, and adding a mulberry leaf extract into the basic culture medium; and S3, adding a CCK-8 reagent, incubating in a CO2 incubator in a dark place for 3 hours, and detecting the proliferation condition of the rat bone marrow mesenchymal stem cells. When the mulberry leaf extract is applied to a basic culture medium, positive regulation and control of cell proliferation and negative regulation and control of a cell apoptosis process can be realized, so that the mulberry leaf extract is applied to preparation of a medicine for preventing and treating periodontitis; and a new experimental basis and a new theoretical basis are provided for research, development and clinical application of the mulberry leaf extract-based periodontal prevention and treatment medicine.

The application relates to a cartilage organoid based on a DNA-silk fibroin hybrid hydrogel sustained-release system and a preparation method and application thereof. The DNA-silk fibroin hybrid hydrogel sustained-release system is a hydrogel system after a DNA-silk fibroin hybrid hydrogel sustained-release system premixing solution is printed through a digital light processing system, wherein the DNA-silk fibroin hybrid hydrogel sustained-release system premixing solution contains DNA, silk fibroin, acrylated RGD peptide, acrylated polyethylene glycol NHS ester, glucosamine, TD-198946 and 2,4,6-trimethylbenzoyl lithium phosphate. Compared with the prior art, the glucosamine and TD-198946 introduced in the DNA-silk fibroin hybrid hydrogel sustained-release system can significantly improve the cartilage differentiation efficiency of bone marrow mesenchymal stem cells; and the application can be transplanted to a cartilage defect as a cartilage graft to promote cartilage regeneration and repair.

The invention belongs to the technical field of biological medicines, and particularly relates to a recombinant polypeptide for improving the activity of bone marrow mesenchymal stem cells and application of the recombinant polypeptide. The amino acid sequence of the recombinant polypeptide is as shown in SEQ ID NO.3. The recombinant polypeptide is obtained by integrating an extracellular matrix amino acid sequence and a heparin amino acid sequence. The recombinant polypeptide provided by the invention can promote the expression of the Ki67 gene and improve the proliferative activity of the bone marrow mesenchymal stem cells, also has the effect of inhibiting the replicative aging of the bone marrow mesenchymal stem cells, and has a wide application prospect.

The application discloses a flavonoid compound in eaglewood as well as a preparation method and application of the flavonoid compound, and relates to the technical field of medicines. The application extracts and separates a new compound from natural eaglewood, and the new compound is used in the preparation of anti-osteoporosis drugs. The compound is detected in vitro to obtain an induction effect of the compound on the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts, so as to affect the formation of osteoclasts. Meanwhile, the compound can promote the bone development of zebra fish through RT-qPCR detection, which indicates that the compound has anti-osteoporosis activity. The separation method of the compound is simple and easy to operate.

The utility model discloses a centrifugal device for extracting mesenchymal stem cells, and relates to the technical field of mesenchymal stem cell analysis, the centrifugal device comprises a device body, a feed inlet and a lifting rod, the inner side of the device body is provided with a lifting plate, and the middle position above the lifting plate is provided with a driving motor; the output end of the driving motor is connected with a centrifugal box through a driving shaft, test tubes are installed above sponge pads, the outer walls of the two sides of the device body are provided with installation holes, ice boxes are installed on the inner sides of the installation holes, and fans are installed above the ice boxes. According to the test tube cooling device, ice blocks are placed in the ice box, then the ice box is installed in the installation hole, the draught fan works to blow air to the ice blocks in the ice box, formed cold air is exhausted through the multiple air outlet pipes, the cold air is exhausted into the device body, test tubes can be cooled conveniently, and the problem that the test tubes are inconvenient to cool is solved.

The invention discloses a bone immunoregulation osteogenesis promoting method of PLL-MgBGs / SF frozen gel and an osteogenesis capability detection method of the PLL-MgBGs / SF frozen gel. The bone immunoregulation osteogenesis promoting method comprises the following steps: preparing a magnesium-doped bioactive substance MgBGs; the preparation method comprises the following steps: preparing PLL-MgBGs nano particles; pLL-MgBGs / SF frozen gel is prepared; a sterile PLL-MgBGs / SF frozen gel leaching solution is prepared; the method comprises the following steps: inoculating macrophages (RAW264.7) into a 48-pore plate, culturing by using a complete culture medium containing LPS (100 ng / mL), and then culturing by using a complete culture medium containing a PLL-MgBGs / SF frozen gel leaching solution. The PLL-MgBGs / SF freezing gel has the advantages that the prepared PLL-MgBGs / SF freezing gel is excellent in water response shape memory ability, capable of releasing various active ions such as Mg < 2 + >, Ca < 2 + > and SiO4 < 4->, remarkable in potential in immunoregulation and bone repair, capable of being implanted into bone defects in an injection mode to fill the bone defects, capable of being rapidly matched with irregular bone defects and capable of improving the bone repair effect. According to the present invention, the problem of insufficient matching of the traditional bone scaffold and the irregular defect site is solved, the excellent mechanical stability and the excellent structure retention can be represented during the degradation process, the good supporting ability is provided in the bone defect region, the polarization of the macrophage can be adjusted to be the M2 type, and the collection, the proliferation and the osteogenic differentiation of the bone marrow mesenchymal stem cells (BMSCs) can be promoted.

Use of extracellular vesicles of Rhizoma Drynariae in the preparation of a medicine for treating orthopedic diseases. The extracellular vesicles are extracted from the traditional Chinese medicine Rhizoma Drynariae, so that proliferation and differentiation of the bone marrow mesenchymal stem cells can be promoted, and the bone targeting property is achieved; in addition, various functional components which are the same as those of Rhizoma Drynariae are contained, so that the potential of treating orthopedic diseases is proved. Therefore, the extracellular vesicles of Rhizoma Drynariae can be applied to the preparation of a medicine for treating orthopedic diseases.

The invention discloses a directional osteogenesis induction skull patch based on an MXene dual-electrode plate and a pulsed direct-current electric field and a preparation method of the directional osteogenesis induction skull patch. The method specifically comprises the following steps: 1) performing intercalation on MXene by using a bacterial cellulose solution as an intercalation agent, and combining a layer-by-layer blade coating and laminating process to obtain a composite MXene electrode plate; (2) preparing a porous GelMA pre-solution loaded with bone marrow mesenchymal stem cells; 3) cutting the composite MXene electrode plate into a proper shape to obtain an upper-layer electrode plate and a lower-layer electrode plate, tiling the lower-layer electrode plate as a substrate, dispensing a cell-loaded porous GelMA pre-solution on the surface of the lower-layer electrode plate, covering the upper-layer electrode plate, and performing gelation by combining lateral UV curing to obtain a double-layer electrode plate composite gel material; and 4) carrying out in-vitro culture on the double-layer electrode plate composite gel material for several days, including electrical stimulation culture and non-electrical stimulation culture, and then stripping the upper electrode plate and the lower electrode plate to obtain the skull patch. According to the skull patch, in-vivo skull defect repair can be achieved, and a brand-new skull regeneration and repair strategy is created.

The invention relates to the technical field of biomedical materials, and discloses a 3D printing stent for bone marrow mesenchymal stem cell culture and application of the 3D printing stent, a zinc-magnesium alloy is used as a base material for laser 3D printing, and then fluorinated coating and fiber coating treatment are performed, so that the biocompatibility and the biological activity of the 3D printing stent are remarkably improved, and the 3D printing stent is suitable for being used for bone marrow mesenchymal stem cell culture. Adhesion and growth of bone marrow mesenchymal stem cells are promoted; the magnesium metal and the zinc metal have mechanical properties similar to those of natural bones, can release magnesium ions and promote osteogenic reaction after being implanted into a body, can be completely degraded into non-toxic substances to be discharged out of the body, and are proved to be potential bone substitute materials; the corrosion resistance of the stent is enhanced by the fluorinated coating, the service life is prolonged, and certain antibacterial and anti-inflammatory characteristics are endowed; sodium alginate and collagen components in the fiber coating layer are beneficial to simulation of an extracellular matrix environment, and cell differentiation and osteogenic reaction are further promoted.