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3D bioprinting ink, preparation method of ink, tissue engineering scaffold and preparation method of scaffold

A tissue engineering scaffold and bioprinting technology, applied in the field of medicine, can solve the problems of increasing the cost of preparation and increasing the complexity of the production process, and achieve the effects of reducing treatment costs, relieving patients' pain, and improving strength

Inactive Publication Date: 2020-01-10
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional bone tissue engineering scaffold materials are usually compounded with growth factors or drugs to improve the bone repair effect of implanted scaffold materials, which not only increases the complexity of the production process, but also increases its preparation cost

Method used

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  • 3D bioprinting ink, preparation method of ink, tissue engineering scaffold and preparation method of scaffold
  • 3D bioprinting ink, preparation method of ink, tissue engineering scaffold and preparation method of scaffold

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preparation example Construction

[0042] A preparation method for 3D bioprinting ink, comprising the following steps:

[0043] S1, weighing raw materials

[0044] Weigh the gelatin particles, sodium alginate powder and lithium magnesium silicate powder according to the set amount, and sterilize and disinfect the gelatin particles, sodium alginate powder and lithium magnesium silicate powder;

[0045] S2, preparation of raw materials

[0046] Dissolve the gelatin particles obtained in S1 with sterile deionized water to obtain a gelatin solution, add sodium alginate powder to the gelatin solution, and obtain a mixed prepolymer solution of gelatin-sodium alginate after dissolving, dissolve the lithium magnesium silicate powder in In sterile deionized water, lithium magnesium silicate colloid was obtained;

[0047] S3, mixed

[0048] Mix the gelatin-sodium alginate mixed prepolymer solution obtained in S2 with the lithium magnesium silicate colloid in equal volumes to obtain a nanocomposite hydrogel, and combin...

Embodiment 1

[0060] The formulations of bioactive tissue engineering bone scaffolds listed in this example include nanocomposite hydrogels and human bone marrow mesenchymal stem cells, wherein the nanocomposite hydrogels include gelatin, sodium alginate, nanoscale lithium magnesium silicate and Deionized water; nanocomposite hydrogel including gelatin 70mg / mL~100mg / mL, sodium alginate 10mg / mL~30mg / mL, magnesium silicate lithium 10mg / mL~30mg / mL, among them, human bone marrow mesenchymal stem cells The volume ratio of the suspension to the nanocomposite hydrogel was 1:400.

[0061] Such as figure 1 Shown, as a preferred embodiment of the present invention, specifically carry out according to the following steps:

[0062] Step 1. Preparation of raw materials: First, put the pre-weighed gelatin granules, sodium alginate powder and lithium magnesium silicate powder in a sterile centrifuge tube, sterilize with ethylene oxide, and set aside; add the gelatin granules to the Put a corresponding v...

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Abstract

The invention discloses 3D bioprinting ink, a preparation method of the ink, a tissue engineering scaffold and a preparation method of the scaffold. The ink contains the following five components: gelatin, sodium alginate, nano-scale magnesium lithium silicate, deionized water and human mesenchymal stem cells. The preparation method of the ink comprises the following steps: firstly dissolving thesterile gelatin and the sodium alginate into the sterile deionized water in order to prepare a mixed prepolymer solution of 140-200 mg / ml gelatin and 20-60 mg / ml sodium alginate; dissolving the sterile magnesium lithium silicate into the sterile deionized water to prepare 20-60 mg / mL magnesium lithium silicate colloid; mixing the two gel in equal volume to prepare a nanocomposite hydrogel which can be used for 3D bioprinting; and finally, uniformly mixing the pre-cultured human mesenchymal stem cells and the nano-composite hydrogel to obtain the nano composite bio-ink with a final cell concentration of 3 x 10<6> / mL. The functionalized, biomimetic tissue engineering bone scaffold with osteogenesis inducing ability is prepared by using the bio-ink as a raw material and adopting a squeeze type 3D bioprinter through printing, and has potential clinical application value

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a 3D bioprinting ink and a preparation method thereof, a tissue engineering scaffold and a preparation method thereof. Background technique [0002] Bone loss due to congenital malformation, trauma, surgical resection, etc. usually requires specific grafts to restore its structure and function. This has always been a worldwide problem in the field of orthopedics, and it has not been effectively resolved so far. At present, autologous or allografts are mostly used clinically, but both methods have problems such as limited bone mass at the donor site, more complications at the donor site, immune rejection, and potential risk of disease transmission. Synthetic biomaterials have attracted extensive attention in recent years as a bone substitute, but due to the lack of biomimetic structure of natural bone tissue, it is difficult to integrate with the bone tissue at the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/02A61L27/20A61L27/22A61L27/58B33Y80/00
CPCA61L27/025A61L27/20A61L27/222A61L27/3834A61L27/58A61L2430/02B33Y80/00C08L5/04C08L89/00
Inventor 刘斌毕龙裴国献宋岳张帅帅
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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