74 results about "3D bioprinting" patented technology

The invention provides a 3D biological printing osteochondral organ based on dynamic mechanical regulation and control, and a construction method and application thereof. Compared with the prior art, the invention innovatively provides a biphasic scaffold optimization modification strategy: firstly, an osteochondral hydrogel scaffold with an accurate layered structure is prepared by adopting a 3D biological printing technology, then a cell mechanical transduction signal channel is activated by a dynamic pressure stimulation system, and cartilage / bone biphasic differentiation of stem cells is directionally regulated and controlled; and by constructing a bionic mechanical microenvironment, the obtained osteochondral organ not only keeps excellent biocompatibility, but also has gradient mechanical properties and a clear spatial layered structure (cartilage layer-bone layer), the directional cartilage formation / osteogenic differentiation efficiency of the osteochondral organ is remarkably improved, and the osteochondral organ has a good application prospect. The problems of spatial heterogeneity construction and accurate regulation and control of cell fate in osteochondral tissue engineering are effectively solved, and the method has important application value in the fields of osteochondral defect repair treatment and mechanical stimulation mechanism research.

The invention relates to bionic bio-ink as well as a preparation method and application thereof, and relates to the technical field of 3D bio-printing. The preparation method of the bionic bio-ink comprises the following steps: sequentially adding methylacryloylated recombinant human collagen, o-nitrobenzyl modified methylacryloylated hyaluronic acid, elastin-like polypeptide and phenyl-2, 4, 6-trimethylbenzoyl lithium phosphonate into a solvent, stirring and uniformly mixing to obtain an ACEM hydrogel precursor solution, and carrying out ultrasonic treatment on the ACEM hydrogel precursor solution to obtain the bionic bio-ink. Uniformly mixing the ACEM hydrogel precursor solution with a photoresist and seed cells to obtain the bionic biological ink. The sequence of the elastin-like polypeptide is as shown in SEQ ID NO. 1. The biological ink is better in mechanical strength, retains the advantages and functions of natural protein, can be quickly photo-cured into a high-strength and dual-network hydrogel structure, and has excellent printability, biocompatibility and cartilage tissue regeneration promoting capacity.

The invention relates to a preparation method of a 3D printing high-density cell tubular vascular graft. The preparation method comprises the following steps: sucking a supernatant obtained by centrifuging a cell suspension away and only remaining bottom pure cells; the preparation method comprises the following steps: uniformly stirring and mixing OMA, pig gelatin and an LAP photoinitiator in a Hank's solution to form a hydrogel precursor solution; extruding the pure cells and the hydrogel precursor solution from a three-axis nozzle by using printing equipment, and immersing the pure cells and the hydrogel precursor solution into a calcium chloride solution; lyase is added to degrade the hydrogel precursor solution, the hydrogel precursor solution is completely degraded, and an independent single-layer pipeline structure with only a remaining pure cell layer is obtained. The invention provides a method for manufacturing high-density pure-cell lumen constructs to mimic native blood vessels by using a self-made microfluidic coaxial 3D bioprinting device. And after the support hydrogel on the inner layer and the outer layer is removed, a single-layer tubular structure of high-density MOVAs only limited to pure cells is obtained. The cells in the pure cell vascular constructs exhibit good cell viability and cell function.

The invention provides a vascularized bone organ and a construction method thereof, and a vascularized bone organ model containing a hollow vascular network is constructed by combining a suspension 3D biological printing method, a cell-carrying printing strategy and a sacrificial biological ink material. The vascularized bone organ model has excellent structural stability, mechanical performance, growth and development capability and osteogenesis capability, selected materials and a manufacturing method are simple in process, engineering stable production can be achieved, cells can be blended for a long time for three-dimensional culture, and the vascularized bone organ model has high applicability and research potential.

The invention discloses a 3D bio-printing sustained-release microneedle patch used after brain glioma surgery and a preparation method thereof.The microneedle patch is prepared by compounding GFD nano-enzyme and a hydrogel base material, the GFD nano-enzyme is gambogic acid-iron-adriamycin nano-enzyme, and the hydrogel base material is a hydrogel base material. The hydrogel base material is composed of photo-crosslinking gelatin (GelMA), methacrylic hyaluronic acid (HAMA), a photoinitiator and an ultraviolet absorbent, the microneedle patch is of a conical microneedle array structure, the interior of the microneedle patch is of a porous structure, and GFD nano-enzyme is evenly distributed in microneedles. The application potential of the existing 3D biological printing and nano-enzyme technology and the technical advantages of 3D biological printing are adopted, by means of the 3D biological printing technology with high-dimensional manufacturing precision, the microneedle array structure is designed and printed in a customized mode according to the irregular shape of the tumor residual cavity of a patient, complete attachment to the wall of the residual cavity is achieved, and the dead cavity effect is solved; meanwhile, the technology can accurately regulate and control the shape, the size and the drug loading capacity of the microneedle, and provides possibility for personalized treatment.

The present invention is concerned with a manufactured article prepared from a biomaterial comprising isolated and purified type I collagen fibrils derived from abalone and a method of preparing such an article. The manufactured article can be prepared by 3D bioprinting, and therefore the present invention also envisages a novel bioink for a 3D bioprinter comprising an isolated and purified type I collagen fibril and cartridges containing such a bioink. The invention additionally relates to a method of isolating type I collagen fibrils from abalone. The manufactured article is useful in a number of biomedical applications including as an implantable device where it can function as a 3D bioprinted hard or soft tissue scaffold for tissue engineering.

The present invention relates to a method for functionalizing a biopolymer having an -OH group or an -NH2 group by reacting the biopolymer with at least one compound having at least one C=C bond or C≡C bond and containing a chromophore that absorbs UV-Vis radiation directly adjacent to this bond. The present invention also relates to a method for obtaining a solid biopolymer material by reversibly crosslinking the functionalized biopolymer obtained by the above method under UV-Vis light irradiation. Furthermore, the present invention relates to the use of functionalized biopolymers as support materials for 3D bioprinting, or as materials for manufacturing structures selected from spheroids, organoids, artificial organs, coatings, and tissue models.

The present disclosure provides a method for bioprinting a 3D structure comprising one or more bio-related materials on a superhydrophobic surface. In one embodiment, the method includes providing a composition having one or more bio-related materials dispersed in a biocompatible medium. A pattern comprising a hydrophilic material is deposited on a defined region of the superhydrophobic surface, the pattern being modeled after a biological structure. A composition having one or more bio-related materials is then bioprinted onto the hydrophilic surface to form a 3D structure, the hydrophilic surface maintaining the 3D structure in a desired position or shape on the superhydrophobic surface.

The invention relates to the technical field of biological engineering, and discloses a method for enhancing the urea circulation function of a 3D biological printing liver organ, which comprises the following steps: a cell preparation step: through a genetic engineering technology, enabling mitochondria of liver cells to express a light-driven proton pump, and enabling intracellular expression of a fluorescent probe for reporting ammonia concentration; an organ construction step: mixing the cells with hydrogel to prepare bio-ink, and constructing a three-dimensional liver organ by using a 3D biological printing technology; a system integration step: placing the liver organ in an integrated photoelectric regulation and control system capable of monitoring a fluorescence signal in real time and applying illumination; and a closed-loop enhancement step: the system monitors the ammonia concentration in real time, and when the ammonia concentration exceeds a preset threshold value, illumination is automatically applied to activate the proton pump, so that the urea circulation function is enhanced as required. Through closed-loop light control and a subcellular organelle energy channel, on-demand, efficient and high-temporal-spatial-resolution dynamic enhancement of the liver organ urea circulation function is achieved.

The invention belongs to the field of 3D biological printing, and particularly relates to hydrogel with a skin injury repairing effect. Aiming at the problem of functional modification of sodium alginate in the existing 3D biological printing technology, the invention provides a preparation method of Eu < 3 + >-mediated collagen polypeptide / sodium alginate composite hydrogel, and the Eu < 3 + >-mediated collagen polypeptide / sodium alginate composite hydrogel is successfully applied to a 3D printing skin wound dressing. The calcium alginate is used as biological ink, the skin full-thickness injury dressing is prepared through a 3D printing technology, and finally the stability of the calcium alginate is enhanced through coordination between Ca < 2 + > and sodium alginate. The 3D printing skin wound dressing constructed by the invention has a good function of promoting skin full-thickness injury repair, and has application potential in the fields of tissue engineering and regenerative medicine.

The invention discloses a quorum sensing quenching enzyme immobilization method of a 3D bio-printing self-assembled nanoflower enzyme-loaded scaffold and application of the quorum sensing quenching enzyme immobilization method. A quorum sensing quenching enzyme YtnP from deep sea is immobilized in an alginate matrix through a 3D printing technology to prepare a QQ scaffold, and the characterization, durability and antifouling performance of a 3D printing composite scaffold material are evaluated. The nanoflower enzyme-loaded scaffold has a continuous AHL degradation effect, and the AHL removal rate exceeds 99.98%. After 220 hours, the compound still has a remarkable inhibiting effect on biological pollution of the raw water PVDF membrane. The quorum sensing quenching enzyme 3D printing support carrying the self-assembled nanoflowers can replace a feeding channel grating, and the service life of a filter membrane subjected to biological scaling in a bioreactor can be remarkably prolonged through biodegradation.

This invention belongs to the technical field of 3D printing of biological tissues and organs, and discloses a biomimetic valve multi-nozzle 3D bioprinted scaffold and its application. The scaffold, from bottom to top, comprises a firmly connected elastin layer, a polysaccharide layer, and a collagen fiber layer. Each layer is constructed by alternating 3D printing of at least two materials; one of these materials is a photocurable hydrogel material providing mechanical support, and the other is a photocurable hydrogel material mixed with bioactive substances. Furthermore, the bioactive substances used in the elastin layer, polysaccharide layer, and collagen fiber layer are different from each other. This invention improves the structure and composition of the 3D bioprinted scaffold, creating a biomimetic three-layer structure and composition for a valve. The scaffold, designed with a three-layer structure of elastin, polysaccharide, and collagen fiber layers, provides cells with a specific sensory microenvironment, stimulating them to secrete corresponding extracellular matrix and completing extracellular matrix remodeling.

The present invention relates to the field of 3D printing technology, and particularly to a 3D bioprinting system, which includes a driving frame, on which a printing component is slidably installed; the printing component includes a printing driving seat and a lifting driving seat, the lifting driving seat is provided with a print head, the print head includes a feeding pipe and a clamping seat, a heat conducting sleeve is arranged at the bottom of the clamping seat, the heat conducting sleeve is connected with a heat conducting plate, and a first heat dissipation fan is installed on the heat conducting plate; fixing seats are respectively installed at both ends of the two clamping seats, heat dissipation grooves are formed in the fixing seats, and second heat dissipation fans are installed in the heat dissipation grooves; the print head can move in six directions, and when sliding, the linear bearing can provide a stable linear path. A large amount of heat is generated during printing. The clamping seat and the heat conducting sleeve can conduct the heat generated by the feeding pipe, and the first heat dissipation fan and the second heat dissipation fan simultaneously discharge the heat outwards, so as to ensure the stability of the bondable material, and the modeling shape printed by the bondable material has a better effect and higher precision.

Bioprinting is the layer-by-layer construction of synthetic tissues or scaffolds. Described herein is a motorized extruder which can precisely extrude and retract extrudate such as bioinks in a compact and rapidly-loadable form-factor. This includes a compact bioprinter using a stepper motor coupled with a threaded shaft to directly move the plunger of an extruder. This pneumatic-mechanical system obviates the needs for pneumatic tubing, rods, or other complex elements of existing designs. The direct drive design further allows for a lighter, smaller gantry that is capable of more precise fabrication of bioprinted constructions. This includes delicate vasculature systems that are beyond limits of existing bioprinting technologies.

Disclosed herein are 3D printed vascular tissues and compositions and methods for making the 3D printed vascular tissues. The 3D printed vascular tissues can be used to model vascular tissue damage (e.g., vascular leakage) and in screening assays for agents that cause or inhibit or treat vascular tissue damage.

This invention discloses a biomimetic decellularized matrix hydrogel and its applications. It relates to the field of biomaterials technology. It comprises the following components: decellularized liver matrix, gelatin, and sodium alginate, and its applications are provided. The therapeutic artificial liver provided by this invention can be mass-produced in vitro using 3D bioprinting technology, exhibiting mature liver functional phenotypes such as ICG uptake / release and drug metabolism in vitro. It was transplanted into a type I tyrosinemia model of liver failure (Fah). ‑ / ‑ It can alleviate liver damage in mice and has the potential for artificial liver transplantation.

Aspects of the disclosure include a fabrication platform for supporting a bioprinted fiber structures during printing, patterning, and / or processing, comprising a frame with a plurality of posts for securing a cross-linkable fiber during printing thereof, and where a continuous length of the cross-linkable fiber is printed around a plurality of posts during the 3D bioprinting process. The fabrication platform enables the cross-linkable fiber to be suspended during one or more of printing, patterning, and / or processing. In this way, the bioprinted fiber structure comprises a uniform outer surface, and can be easily modified and / or further processed after printing and patterning are completed.

The application discloses a kind of 3D biological printing engineering green algae active material and preparation method thereof, active material includes the composite layer of a plurality of laminated settings, the composite layer includes: carrier layer, and its above biological ink layer;The mass ratio of carrier layer and biological ink layer is 1:1-1:2;The biological ink layer includes: hydrogel, culture medium and upconversion nanomaterial modified green algae cell;The emission wavelength of upconversion nanomaterial is in the excitation wavelength range of green algae chloroplast.This 3D biological printing engineering green algae active material of the application contains upconversion nanomaterial modified green algae cell, under near infrared light condition, upconversion nanomaterial can be excited to visible light by near infrared light, for green algae photosynthesis to produce oxygen;Chlorophyll a (photosensitizer) in green algae can be excited to excited state by the above visible light, and react with the above oxygen, produce reactive oxygen species (such as singlet oxygen 1 O2), induce cancer cell death.

The invention relates to the technical field of biomedical engineering, in particular to a pancreatic cancer organoid model and a construction method and application thereof. The construction method comprises the following steps: extracting cancer cells from a pancreatic cancer tissue sample, preparing first printing ink, printing by a liquid drop method, curing and culturing an obtained first printing structural body, and preparing the organoid. And recovering cancer cells in the organoid, preparing second printing ink, printing by a 3D biological printing method, and curing and culturing an obtained second printing structure to prepare the pancreatic cancer organoid model. According to the novel pancreatic cancer organ model construction method provided by the invention, a 3D biological printing technology is introduced, high-flux printing of the pancreatic cancer organ model is realized, and the constructed pancreatic cancer organ model has high fidelity compared with an original tumor tissue; the pancreatic cancer organoid model can be used for accurately predicting the sensitivity of cancer cell-derived individuals to anti-pancreatic cancer drugs.

An apparatus controls a series of actuators mounted onto inverted microscope assemblies to add 3D bioprinting and pick and place capabilities on a microscopic scale. One or more syringes are also mounted to the apparatus and the plunger of each syringe is independently actuated to extrude or extract fluids from the optically aligned needle. A software package communicates with the microscope x, y stage as well as the on-board motor controller which actuates the syringes and needle to set the x, y, z position and syringe in real time.

Aspects of the present disclosure include a manufacturing platform for supporting bioprinted fibrous structures during printing, patterning and / or processing, the manufacturing platform including a frame having a plurality of columns for securing crosslinkable fibers during printing of the crosslinkable fibers, and wherein a continuous length of the crosslinkable fiber is printed around a plurality of columns during a 3D bioprinting process. The manufacturing platform enables the crosslinkable fibers to be suspended during one or more of printing, patterning, and / or processing. In this manner, the bioprinted fibrous structure includes a uniform outer surface and may be readily tailored and / or further processed after printing and patterning are completed.

3D printing assembly 10, suitable for bioprinting, the assembly including an enclosure 100 defining an enclosure internal area 101, a printer 200 enclosed within the internal area, the printer compris

The present invention provides a device for 3D bioprinting in vivo, the device comprising an elongated hollow tube configured to be insertable into a living body. A feed tube within the hollow tube delivers the liquid polymerizable biological material to an extrusion nozzle positioned at a target site. A light guide within the hollow tube conducts polymerized light from a light source to polymerize the biological material that has been extruded at the target site. Arthroscopic procedures employing the device enable attachment of tissue to bone or other tissue in vivo, or replacement of missing tissue or bone volume.

The invention relates to an aqueous composition comprising thiolated and norbornene-modified cold- water species-derived gelatine. A further aspect of the invention relates to a method to generate a three-dimensional hydrogel object, particularly an in vitro model for disease modelling / drug testing or an implant, comprising providing a composition according to the invention and forming the hydrogel object by bioprinting (extrusion-based or digital light-based (DLP)) or molding / casting to provide the three-dimensional hydrogel object. Also provided are a hydrogel implant obtained by a method according to the invention, and a kit comprising dried thiolated and norbornene-modified cold-water marine species gelatine.

An integrally-formed three-dimensional (3D) bio-printing system capable of alternate feeding of multiple materials, comprising: an optical imaging unit and a light path conversion unit, wherein the optical imaging unit comprises an image processing unit and a projection unit, the image processing unit segmenting a 3D modeling graphic of a printed subject to form image information, the projection unit converting the image information into one or more optical images, and the light path conversion unit projects the imaged light paths into bio-ink that can be cured by light, so that the projected image can cure the bio-ink by means of the focus of light.

A method for manufacturing a patient-customized wound dressing integrated with microneedles, and a patient-customized wound dressing integrated with microneedles, manufactured through same, are provided. A wound dressing is manufactured through 3D bioprinting by using a photopolymerizable biocompatible biodegradable bioink, and then microneedles are formed on the wound dressing through two-photon polymerization (2PP) printing by using the photopolymerizable biocompatible biodegradable bioink, and thus the patient-customized wound dressing integrated with microneedles is manufactured.

The invention relates to a composition for preparing a 3D scaffold for culturing cells and tissue, such as human cells and tissue. In particular embodiments, the present invention relates to a composition comprising a biocompatible polymer suitable for the preparation of a hydrogel, and a modified extracellular matrix (ECM) protein that is unreactive towards the biocompatible polymer, such that, after preparation of a hydrogel, the modified ECM protein is not covalently bound to the hydrogel. Compositions of the invention are suitable for use in 3D bioprinting, tissue engineering, drug screening, disease modelling and methods of treatment such as tissue regeneration.

The invention belongs to the technical field of construction of biological models, and particularly relates to application of etomidate as a modeling inducer of a Parkinson's disease model. The invention proves that etomidate can be used as a novel Parkinson's disease modeling inducer through a dual verification system of a humanized neural model and an animal model based on biological 3D printing.

The invention relates to a 3D biological printing manufacturing device for multi-process composite forming. The 3D biological printing manufacturing device mainly comprises a rack, a spray head printing system and a forming platform system. The printing system can achieve extrusion biological printing and electrostatic spinning and mainly comprises a Y-direction sliding rail, a sliding block, a gantry, an X-direction sliding rail, a magnetic suction cup, an electrostatic spinning spray head, an extrusion printing spray head, a DLP light source and a DLP light source suspension. The forming platform system can move in the Z-axis direction and mainly comprises a table top, a material groove, a Z-direction sliding rail, a receiving platform, a platform clamp, a Z-axis sliding block, a driving device, a square hole and a positioning hole. Compared with a single forming mode, the multi-process composite forming biological manufacturing device can achieve multi-material and multi-scale integrated forming printing of a complex tissue system.

The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.