59 results about "3D bioprinting" patented technology

The invention relates to a preparation method of a 3D printing high-density cell tubular vascular graft. The preparation method comprises the following steps: sucking a supernatant obtained by centrifuging a cell suspension away and only remaining bottom pure cells; the preparation method comprises the following steps: uniformly stirring and mixing OMA, pig gelatin and an LAP photoinitiator in a Hank's solution to form a hydrogel precursor solution; extruding the pure cells and the hydrogel precursor solution from a three-axis nozzle by using printing equipment, and immersing the pure cells and the hydrogel precursor solution into a calcium chloride solution; lyase is added to degrade the hydrogel precursor solution, the hydrogel precursor solution is completely degraded, and an independent single-layer pipeline structure with only a remaining pure cell layer is obtained. The invention provides a method for manufacturing high-density pure-cell lumen constructs to mimic native blood vessels by using a self-made microfluidic coaxial 3D bioprinting device. And after the support hydrogel on the inner layer and the outer layer is removed, a single-layer tubular structure of high-density MOVAs only limited to pure cells is obtained. The cells in the pure cell vascular constructs exhibit good cell viability and cell function.

The invention provides a vascularized bone organ and a construction method thereof, and a vascularized bone organ model containing a hollow vascular network is constructed by combining a suspension 3D biological printing method, a cell-carrying printing strategy and a sacrificial biological ink material. The vascularized bone organ model has excellent structural stability, mechanical performance, growth and development capability and osteogenesis capability, selected materials and a manufacturing method are simple in process, engineering stable production can be achieved, cells can be blended for a long time for three-dimensional culture, and the vascularized bone organ model has high applicability and research potential.

The invention discloses a 3D bio-printing sustained-release microneedle patch used after brain glioma surgery and a preparation method thereof.The microneedle patch is prepared by compounding GFD nano-enzyme and a hydrogel base material, the GFD nano-enzyme is gambogic acid-iron-adriamycin nano-enzyme, and the hydrogel base material is a hydrogel base material. The hydrogel base material is composed of photo-crosslinking gelatin (GelMA), methacrylic hyaluronic acid (HAMA), a photoinitiator and an ultraviolet absorbent, the microneedle patch is of a conical microneedle array structure, the interior of the microneedle patch is of a porous structure, and GFD nano-enzyme is evenly distributed in microneedles. The application potential of the existing 3D biological printing and nano-enzyme technology and the technical advantages of 3D biological printing are adopted, by means of the 3D biological printing technology with high-dimensional manufacturing precision, the microneedle array structure is designed and printed in a customized mode according to the irregular shape of the tumor residual cavity of a patient, complete attachment to the wall of the residual cavity is achieved, and the dead cavity effect is solved; meanwhile, the technology can accurately regulate and control the shape, the size and the drug loading capacity of the microneedle, and provides possibility for personalized treatment.

The present invention is concerned with a manufactured article prepared from a biomaterial comprising isolated and purified type I collagen fibrils derived from abalone and a method of preparing such an article. The manufactured article can be prepared by 3D bioprinting, and therefore the present invention also envisages a novel bioink for a 3D bioprinter comprising an isolated and purified type I collagen fibril and cartridges containing such a bioink. The invention additionally relates to a method of isolating type I collagen fibrils from abalone. The manufactured article is useful in a number of biomedical applications including as an implantable device where it can function as a 3D bioprinted hard or soft tissue scaffold for tissue engineering.

The present invention relates to a method for functionalizing a biopolymer having an -OH group or an -NH2 group by reacting the biopolymer with at least one compound having at least one C=C bond or C≡C bond and containing a chromophore that absorbs UV-Vis radiation directly adjacent to this bond. The present invention also relates to a method for obtaining a solid biopolymer material by reversibly crosslinking the functionalized biopolymer obtained by the above method under UV-Vis light irradiation. Furthermore, the present invention relates to the use of functionalized biopolymers as support materials for 3D bioprinting, or as materials for manufacturing structures selected from spheroids, organoids, artificial organs, coatings, and tissue models.

The present disclosure provides a method for bioprinting a 3D structure comprising one or more bio-related materials on a superhydrophobic surface. In one embodiment, the method includes providing a composition having one or more bio-related materials dispersed in a biocompatible medium. A pattern comprising a hydrophilic material is deposited on a defined region of the superhydrophobic surface, the pattern being modeled after a biological structure. A composition having one or more bio-related materials is then bioprinted onto the hydrophilic surface to form a 3D structure, the hydrophilic surface maintaining the 3D structure in a desired position or shape on the superhydrophobic surface.

The invention relates to the technical field of biological engineering, and discloses a method for enhancing the urea circulation function of a 3D biological printing liver organ, which comprises the following steps: a cell preparation step: through a genetic engineering technology, enabling mitochondria of liver cells to express a light-driven proton pump, and enabling intracellular expression of a fluorescent probe for reporting ammonia concentration; an organ construction step: mixing the cells with hydrogel to prepare bio-ink, and constructing a three-dimensional liver organ by using a 3D biological printing technology; a system integration step: placing the liver organ in an integrated photoelectric regulation and control system capable of monitoring a fluorescence signal in real time and applying illumination; and a closed-loop enhancement step: the system monitors the ammonia concentration in real time, and when the ammonia concentration exceeds a preset threshold value, illumination is automatically applied to activate the proton pump, so that the urea circulation function is enhanced as required. Through closed-loop light control and a subcellular organelle energy channel, on-demand, efficient and high-temporal-spatial-resolution dynamic enhancement of the liver organ urea circulation function is achieved.

The invention discloses a quorum sensing quenching enzyme immobilization method of a 3D bio-printing self-assembled nanoflower enzyme-loaded scaffold and application of the quorum sensing quenching enzyme immobilization method. A quorum sensing quenching enzyme YtnP from deep sea is immobilized in an alginate matrix through a 3D printing technology to prepare a QQ scaffold, and the characterization, durability and antifouling performance of a 3D printing composite scaffold material are evaluated. The nanoflower enzyme-loaded scaffold has a continuous AHL degradation effect, and the AHL removal rate exceeds 99.98%. After 220 hours, the compound still has a remarkable inhibiting effect on biological pollution of the raw water PVDF membrane. The quorum sensing quenching enzyme 3D printing support carrying the self-assembled nanoflowers can replace a feeding channel grating, and the service life of a filter membrane subjected to biological scaling in a bioreactor can be remarkably prolonged through biodegradation.

This invention belongs to the technical field of 3D printing of biological tissues and organs, and discloses a biomimetic valve multi-nozzle 3D bioprinted scaffold and its application. The scaffold, from bottom to top, comprises a firmly connected elastin layer, a polysaccharide layer, and a collagen fiber layer. Each layer is constructed by alternating 3D printing of at least two materials; one of these materials is a photocurable hydrogel material providing mechanical support, and the other is a photocurable hydrogel material mixed with bioactive substances. Furthermore, the bioactive substances used in the elastin layer, polysaccharide layer, and collagen fiber layer are different from each other. This invention improves the structure and composition of the 3D bioprinted scaffold, creating a biomimetic three-layer structure and composition for a valve. The scaffold, designed with a three-layer structure of elastin, polysaccharide, and collagen fiber layers, provides cells with a specific sensory microenvironment, stimulating them to secrete corresponding extracellular matrix and completing extracellular matrix remodeling.

The present invention relates to the field of 3D printing technology, and particularly to a 3D bioprinting system, which includes a driving frame, on which a printing component is slidably installed; the printing component includes a printing driving seat and a lifting driving seat, the lifting driving seat is provided with a print head, the print head includes a feeding pipe and a clamping seat, a heat conducting sleeve is arranged at the bottom of the clamping seat, the heat conducting sleeve is connected with a heat conducting plate, and a first heat dissipation fan is installed on the heat conducting plate; fixing seats are respectively installed at both ends of the two clamping seats, heat dissipation grooves are formed in the fixing seats, and second heat dissipation fans are installed in the heat dissipation grooves; the print head can move in six directions, and when sliding, the linear bearing can provide a stable linear path. A large amount of heat is generated during printing. The clamping seat and the heat conducting sleeve can conduct the heat generated by the feeding pipe, and the first heat dissipation fan and the second heat dissipation fan simultaneously discharge the heat outwards, so as to ensure the stability of the bondable material, and the modeling shape printed by the bondable material has a better effect and higher precision.

Disclosed herein are 3D printed vascular tissues and compositions and methods for making the 3D printed vascular tissues. The 3D printed vascular tissues can be used to model vascular tissue damage (e.g., vascular leakage) and in screening assays for agents that cause or inhibit or treat vascular tissue damage.

This invention discloses a biomimetic decellularized matrix hydrogel and its applications. It relates to the field of biomaterials technology. It comprises the following components: decellularized liver matrix, gelatin, and sodium alginate, and its applications are provided. The therapeutic artificial liver provided by this invention can be mass-produced in vitro using 3D bioprinting technology, exhibiting mature liver functional phenotypes such as ICG uptake / release and drug metabolism in vitro. It was transplanted into a type I tyrosinemia model of liver failure (Fah). ‑ / ‑ It can alleviate liver damage in mice and has the potential for artificial liver transplantation.

Aspects of the disclosure include a fabrication platform for supporting a bioprinted fiber structures during printing, patterning, and / or processing, comprising a frame with a plurality of posts for securing a cross-linkable fiber during printing thereof, and where a continuous length of the cross-linkable fiber is printed around a plurality of posts during the 3D bioprinting process. The fabrication platform enables the cross-linkable fiber to be suspended during one or more of printing, patterning, and / or processing. In this way, the bioprinted fiber structure comprises a uniform outer surface, and can be easily modified and / or further processed after printing and patterning are completed.

The application discloses a kind of 3D biological printing engineering green algae active material and preparation method thereof, active material includes the composite layer of a plurality of laminated settings, the composite layer includes: carrier layer, and its above biological ink layer;The mass ratio of carrier layer and biological ink layer is 1:1-1:2;The biological ink layer includes: hydrogel, culture medium and upconversion nanomaterial modified green algae cell;The emission wavelength of upconversion nanomaterial is in the excitation wavelength range of green algae chloroplast.This 3D biological printing engineering green algae active material of the application contains upconversion nanomaterial modified green algae cell, under near infrared light condition, upconversion nanomaterial can be excited to visible light by near infrared light, for green algae photosynthesis to produce oxygen;Chlorophyll a (photosensitizer) in green algae can be excited to excited state by the above visible light, and react with the above oxygen, produce reactive oxygen species (such as singlet oxygen 1 O2), induce cancer cell death.

The invention relates to the technical field of biomedical engineering, in particular to a pancreatic cancer organoid model and a construction method and application thereof. The construction method comprises the following steps: extracting cancer cells from a pancreatic cancer tissue sample, preparing first printing ink, printing by a liquid drop method, curing and culturing an obtained first printing structural body, and preparing the organoid. And recovering cancer cells in the organoid, preparing second printing ink, printing by a 3D biological printing method, and curing and culturing an obtained second printing structure to prepare the pancreatic cancer organoid model. According to the novel pancreatic cancer organ model construction method provided by the invention, a 3D biological printing technology is introduced, high-flux printing of the pancreatic cancer organ model is realized, and the constructed pancreatic cancer organ model has high fidelity compared with an original tumor tissue; the pancreatic cancer organoid model can be used for accurately predicting the sensitivity of cancer cell-derived individuals to anti-pancreatic cancer drugs.

Aspects of the present disclosure include a manufacturing platform for supporting bioprinted fibrous structures during printing, patterning and / or processing, the manufacturing platform including a frame having a plurality of columns for securing crosslinkable fibers during printing of the crosslinkable fibers, and wherein a continuous length of the crosslinkable fiber is printed around a plurality of columns during a 3D bioprinting process. The manufacturing platform enables the crosslinkable fibers to be suspended during one or more of printing, patterning, and / or processing. In this manner, the bioprinted fibrous structure includes a uniform outer surface and may be readily tailored and / or further processed after printing and patterning are completed.

The invention relates to an aqueous composition comprising thiolated and norbornene-modified cold- water species-derived gelatine. A further aspect of the invention relates to a method to generate a three-dimensional hydrogel object, particularly an in vitro model for disease modelling / drug testing or an implant, comprising providing a composition according to the invention and forming the hydrogel object by bioprinting (extrusion-based or digital light-based (DLP)) or molding / casting to provide the three-dimensional hydrogel object. Also provided are a hydrogel implant obtained by a method according to the invention, and a kit comprising dried thiolated and norbornene-modified cold-water marine species gelatine.

An integrally-formed three-dimensional (3D) bio-printing system capable of alternate feeding of multiple materials, comprising: an optical imaging unit and a light path conversion unit, wherein the optical imaging unit comprises an image processing unit and a projection unit, the image processing unit segmenting a 3D modeling graphic of a printed subject to form image information, the projection unit converting the image information into one or more optical images, and the light path conversion unit projects the imaged light paths into bio-ink that can be cured by light, so that the projected image can cure the bio-ink by means of the focus of light.

The invention belongs to the technical field of construction of biological models, and particularly relates to application of etomidate as a modeling inducer of a Parkinson's disease model. The invention proves that etomidate can be used as a novel Parkinson's disease modeling inducer through a dual verification system of a humanized neural model and an animal model based on biological 3D printing.

The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

A controller (300, figure 4) for a 3D bioprinter 10, the controller being operable to control the functioning of the 3D bioprinter housed within an internal area of an enclosure 100, to produce a 3D p

The invention relates to the field of organic photocuring materials, in particular to a photoinitiator and a preparation method thereof. The photoinitiator successfully overcomes the problems of single function, limited applicability, lack of antibacterial property and the like in the existing glycosyl modified photoinitiator through a dual modification method. On the whole, according to the technical scheme, the efficient photo-initiation capacity and the excellent photosensitive characteristic of the hydroxyl ketone photoinitiator are kept, and the biocompatibility advantage of glycosyl modification and the antibacterial / antistatic multifunctionality of quaternary ammonium salt modification are combined. The photoinitiator not only can be compatible with photoplatemaking of pattern surfaces in various printing processes, but also can be used for bio-ink to realize 3D bio-printing, and has special significance for use scenes with higher requirements on biocompatibility; the method has great application potential and market promotion value in various high-end fields such as water-based coatings, medical dressings, food contact materials, biomedical hydrogel and the like.

The invention relates to a method for obtaining a dental prosthesis comprising a crown and a root. The method comprises at least one step of guiding undifferentiated cells or keratinocytes and at least one step of three-dimensional printing, preferably 3D bioprinting of the dental prosthesis. Furthermore, the invention relates to a dental prosthesis obtained using said method, as well as to bio-inks suitable for three-dimensional printing (3D), preferably for 3D bioprinting.

A heterogeneous component free synthetic bio-ink formulation comprising a polymer derived from poly (asparagine) (PASPAm) for 3D bioprinting applications, a method of producing a three-dimensional object using the synthetic bio-ink formulation, and a three-dimensional object obtainable by the method are provided.

This relates to a hydrogel precursor composition and to a bioink obtainable from the hydrogel precursor composition, as well as a method of preparing and using the same. More specifically, This also relates to a bioink that contains a catechol grafted and spontaneously crosslinked hydrogel including cells, and to its use in 3D bioprinting and to a method of preparing the same.

The application discloses a 3D bioprinted radial porous scaffold for staged bone regeneration and a preparation method thereof, the 3D bioprinted radial porous scaffold can provide a biomimetic cell microenvironment to support the infiltration of host repair cells to a defect site, and promote bone integration after implantation, in addition, the sequential release of double growth factors and the sustained release of PO4 3‑ and Ca 2+ in the 3D bioprinted radial porous scaffold can complete the three key steps of staged bone regeneration, can realize efficient bone regeneration, and represents a promising biomimetic staged regeneration strategy.

The present invention provides a 3D bioprinting ink and a method for preparing a cell culture scaffold. Among them, the 3D bioprinting ink uses nano-starch as a functional factor, greatly enhancing the 3D printing properties of the hydrogel under low-temperature conditions and significantly promoting the adhesion and proliferation of fish myoblasts. The cell culture scaffold printed based on this ink can effectively promote the adhesion and proliferation of myoblasts compared with ordinary gelatin scaffolds. The nano-starch, gelatin, and sodium alginate of the present invention are all food-grade raw materials and have great application prospects in the field of cultured meat.

The invention relates to a method for functionalizing a biopolymer, in which a biopolymer having-OH or-NH2 groups is reacted with at least one compound containing at least one C = C or C = C bond and a UV-Vis radiation-absorbing chromophore directly adjacent to the bond. The invention also relates to a process for obtaining a solid biopolymer material, wherein the functionalized biopolymer obtained by the above process undergoes reversible crosslinking upon exposure to light in the UV-VIS range. The invention also relates to the use of the functionalized biopolymer as a support material for 3D bioprinting and to the use of the functionalized biopolymer for producing a structure selected from spheroids, organoids, artificial organs, envelopes and tissue models.

This invention provides a water-soluble synthetic polymer bio-ink, comprising a dispersed phase and a dispersant mixed together. The dispersant is water, and the dispersed phase includes polymer microspheres, a crosslinking agent, and a UV photoinitiator. The polymer microspheres are made of a synthetic polymer material modified with double bonds at both ends. This water-soluble synthetic polymer bio-ink can be used for 3D bioprinting in a water-soluble system. This invention also provides a method for preparing the water-soluble synthetic polymer bio-ink, which is simple, low-cost, and suitable for industrial production. Furthermore, this invention provides an application of the water-soluble synthetic polymer bio-ink, which allows for 3D bioprinting and solidification in a water-soluble system to obtain a structurally stable and well-formed 3D printed scaffold. Cell fluid can also be added to the water-soluble synthetic polymer bio-ink to print a cell-containing 3D printed scaffold.

A method of rapid constructing human cerebral cortical organoids by 3D bioprinting and an application including preparing microfluidic chips, preparation of hydrogel of human cerebral cortical organoids, and printing of human cerebral cortical organoids. The microfluidic chip comprises a mixed-flow channel layer, liquid pool layer, microporous array layer, human cerebral cortical organoid culture layer, and culture medium recovery layer; the human cerebral cortical organoid hydrogel has gelatin, alginate, and hyaluronic acid; printing directly human cerebral cortical organoids in microfluidic chips by FRESH printing method, obtaining human cerebral cortical organoid chips after packaging. The application directly constructs large-scale human cerebral cortex-like with three layers of mutually connected structures in situ in organ chip through 3D bioprinting, simulates cerebrospinal fluid circulation through perfusion culture.