50 results about "3D bioprinting" patented technology

The invention provides a vascularized bone organ and a construction method thereof, and a vascularized bone organ model containing a hollow vascular network is constructed by combining a suspension 3D biological printing method, a cell-carrying printing strategy and a sacrificial biological ink material. The vascularized bone organ model has excellent structural stability, mechanical performance, growth and development capability and osteogenesis capability, selected materials and a manufacturing method are simple in process, engineering stable production can be achieved, cells can be blended for a long time for three-dimensional culture, and the vascularized bone organ model has high applicability and research potential.

The invention discloses a 3D bio-printing sustained-release microneedle patch used after brain glioma surgery and a preparation method thereof.The microneedle patch is prepared by compounding GFD nano-enzyme and a hydrogel base material, the GFD nano-enzyme is gambogic acid-iron-adriamycin nano-enzyme, and the hydrogel base material is a hydrogel base material. The hydrogel base material is composed of photo-crosslinking gelatin (GelMA), methacrylic hyaluronic acid (HAMA), a photoinitiator and an ultraviolet absorbent, the microneedle patch is of a conical microneedle array structure, the interior of the microneedle patch is of a porous structure, and GFD nano-enzyme is evenly distributed in microneedles. The application potential of the existing 3D biological printing and nano-enzyme technology and the technical advantages of 3D biological printing are adopted, by means of the 3D biological printing technology with high-dimensional manufacturing precision, the microneedle array structure is designed and printed in a customized mode according to the irregular shape of the tumor residual cavity of a patient, complete attachment to the wall of the residual cavity is achieved, and the dead cavity effect is solved; meanwhile, the technology can accurately regulate and control the shape, the size and the drug loading capacity of the microneedle, and provides possibility for personalized treatment.

The present invention is concerned with a manufactured article prepared from a biomaterial comprising isolated and purified type I collagen fibrils derived from abalone and a method of preparing such an article. The manufactured article can be prepared by 3D bioprinting, and therefore the present invention also envisages a novel bioink for a 3D bioprinter comprising an isolated and purified type I collagen fibril and cartridges containing such a bioink. The invention additionally relates to a method of isolating type I collagen fibrils from abalone. The manufactured article is useful in a number of biomedical applications including as an implantable device where it can function as a 3D bioprinted hard or soft tissue scaffold for tissue engineering.

The present invention relates to a method for functionalizing a biopolymer having an -OH group or an -NH2 group by reacting the biopolymer with at least one compound having at least one C=C bond or C≡C bond and containing a chromophore that absorbs UV-Vis radiation directly adjacent to this bond. The present invention also relates to a method for obtaining a solid biopolymer material by reversibly crosslinking the functionalized biopolymer obtained by the above method under UV-Vis light irradiation. Furthermore, the present invention relates to the use of functionalized biopolymers as support materials for 3D bioprinting, or as materials for manufacturing structures selected from spheroids, organoids, artificial organs, coatings, and tissue models.

The invention relates to the technical field of biological engineering, and discloses a method for enhancing the urea circulation function of a 3D biological printing liver organ, which comprises the following steps: a cell preparation step: through a genetic engineering technology, enabling mitochondria of liver cells to express a light-driven proton pump, and enabling intracellular expression of a fluorescent probe for reporting ammonia concentration; an organ construction step: mixing the cells with hydrogel to prepare bio-ink, and constructing a three-dimensional liver organ by using a 3D biological printing technology; a system integration step: placing the liver organ in an integrated photoelectric regulation and control system capable of monitoring a fluorescence signal in real time and applying illumination; and a closed-loop enhancement step: the system monitors the ammonia concentration in real time, and when the ammonia concentration exceeds a preset threshold value, illumination is automatically applied to activate the proton pump, so that the urea circulation function is enhanced as required. Through closed-loop light control and a subcellular organelle energy channel, on-demand, efficient and high-temporal-spatial-resolution dynamic enhancement of the liver organ urea circulation function is achieved.

The invention discloses a quorum sensing quenching enzyme immobilization method of a 3D bio-printing self-assembled nanoflower enzyme-loaded scaffold and application of the quorum sensing quenching enzyme immobilization method. A quorum sensing quenching enzyme YtnP from deep sea is immobilized in an alginate matrix through a 3D printing technology to prepare a QQ scaffold, and the characterization, durability and antifouling performance of a 3D printing composite scaffold material are evaluated. The nanoflower enzyme-loaded scaffold has a continuous AHL degradation effect, and the AHL removal rate exceeds 99.98%. After 220 hours, the compound still has a remarkable inhibiting effect on biological pollution of the raw water PVDF membrane. The quorum sensing quenching enzyme 3D printing support carrying the self-assembled nanoflowers can replace a feeding channel grating, and the service life of a filter membrane subjected to biological scaling in a bioreactor can be remarkably prolonged through biodegradation.

This invention belongs to the technical field of 3D printing of biological tissues and organs, and discloses a biomimetic valve multi-nozzle 3D bioprinted scaffold and its application. The scaffold, from bottom to top, comprises a firmly connected elastin layer, a polysaccharide layer, and a collagen fiber layer. Each layer is constructed by alternating 3D printing of at least two materials; one of these materials is a photocurable hydrogel material providing mechanical support, and the other is a photocurable hydrogel material mixed with bioactive substances. Furthermore, the bioactive substances used in the elastin layer, polysaccharide layer, and collagen fiber layer are different from each other. This invention improves the structure and composition of the 3D bioprinted scaffold, creating a biomimetic three-layer structure and composition for a valve. The scaffold, designed with a three-layer structure of elastin, polysaccharide, and collagen fiber layers, provides cells with a specific sensory microenvironment, stimulating them to secrete corresponding extracellular matrix and completing extracellular matrix remodeling.

Disclosed herein are 3D printed vascular tissues and compositions and methods for making the 3D printed vascular tissues. The 3D printed vascular tissues can be used to model vascular tissue damage (e.g., vascular leakage) and in screening assays for agents that cause or inhibit or treat vascular tissue damage.

This invention discloses a biomimetic decellularized matrix hydrogel and its applications. It relates to the field of biomaterials technology. It comprises the following components: decellularized liver matrix, gelatin, and sodium alginate, and its applications are provided. The therapeutic artificial liver provided by this invention can be mass-produced in vitro using 3D bioprinting technology, exhibiting mature liver functional phenotypes such as ICG uptake / release and drug metabolism in vitro. It was transplanted into a type I tyrosinemia model of liver failure (Fah). ‑ / ‑ It can alleviate liver damage in mice and has the potential for artificial liver transplantation.

Aspects of the disclosure include a fabrication platform for supporting a bioprinted fiber structures during printing, patterning, and / or processing, comprising a frame with a plurality of posts for securing a cross-linkable fiber during printing thereof, and where a continuous length of the cross-linkable fiber is printed around a plurality of posts during the 3D bioprinting process. The fabrication platform enables the cross-linkable fiber to be suspended during one or more of printing, patterning, and / or processing. In this way, the bioprinted fiber structure comprises a uniform outer surface, and can be easily modified and / or further processed after printing and patterning are completed.

The application discloses a kind of 3D biological printing engineering green algae active material and preparation method thereof, active material includes the composite layer of a plurality of laminated settings, the composite layer includes: carrier layer, and its above biological ink layer;The mass ratio of carrier layer and biological ink layer is 1:1-1:2;The biological ink layer includes: hydrogel, culture medium and upconversion nanomaterial modified green algae cell;The emission wavelength of upconversion nanomaterial is in the excitation wavelength range of green algae chloroplast.This 3D biological printing engineering green algae active material of the application contains upconversion nanomaterial modified green algae cell, under near infrared light condition, upconversion nanomaterial can be excited to visible light by near infrared light, for green algae photosynthesis to produce oxygen;Chlorophyll a (photosensitizer) in green algae can be excited to excited state by the above visible light, and react with the above oxygen, produce reactive oxygen species (such as singlet oxygen 1 O2), induce cancer cell death.

The invention relates to the technical field of biomedical engineering, in particular to a pancreatic cancer organoid model and a construction method and application thereof. The construction method comprises the following steps: extracting cancer cells from a pancreatic cancer tissue sample, preparing first printing ink, printing by a liquid drop method, curing and culturing an obtained first printing structural body, and preparing the organoid. And recovering cancer cells in the organoid, preparing second printing ink, printing by a 3D biological printing method, and curing and culturing an obtained second printing structure to prepare the pancreatic cancer organoid model. According to the novel pancreatic cancer organ model construction method provided by the invention, a 3D biological printing technology is introduced, high-flux printing of the pancreatic cancer organ model is realized, and the constructed pancreatic cancer organ model has high fidelity compared with an original tumor tissue; the pancreatic cancer organoid model can be used for accurately predicting the sensitivity of cancer cell-derived individuals to anti-pancreatic cancer drugs.

The invention relates to an aqueous composition comprising thiolated and norbornene-modified cold- water species-derived gelatine. A further aspect of the invention relates to a method to generate a three-dimensional hydrogel object, particularly an in vitro model for disease modelling / drug testing or an implant, comprising providing a composition according to the invention and forming the hydrogel object by bioprinting (extrusion-based or digital light-based (DLP)) or molding / casting to provide the three-dimensional hydrogel object. Also provided are a hydrogel implant obtained by a method according to the invention, and a kit comprising dried thiolated and norbornene-modified cold-water marine species gelatine.

An integrally-formed three-dimensional (3D) bio-printing system capable of alternate feeding of multiple materials, comprising: an optical imaging unit and a light path conversion unit, wherein the optical imaging unit comprises an image processing unit and a projection unit, the image processing unit segmenting a 3D modeling graphic of a printed subject to form image information, the projection unit converting the image information into one or more optical images, and the light path conversion unit projects the imaged light paths into bio-ink that can be cured by light, so that the projected image can cure the bio-ink by means of the focus of light.

The invention belongs to the technical field of construction of biological models, and particularly relates to application of etomidate as a modeling inducer of a Parkinson's disease model. The invention proves that etomidate can be used as a novel Parkinson's disease modeling inducer through a dual verification system of a humanized neural model and an animal model based on biological 3D printing.

The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

A controller (300, figure 4) for a 3D bioprinter 10, the controller being operable to control the functioning of the 3D bioprinter housed within an internal area of an enclosure 100, to produce a 3D p

The invention relates to the field of organic photocuring materials, in particular to a photoinitiator and a preparation method thereof. The photoinitiator successfully overcomes the problems of single function, limited applicability, lack of antibacterial property and the like in the existing glycosyl modified photoinitiator through a dual modification method. On the whole, according to the technical scheme, the efficient photo-initiation capacity and the excellent photosensitive characteristic of the hydroxyl ketone photoinitiator are kept, and the biocompatibility advantage of glycosyl modification and the antibacterial / antistatic multifunctionality of quaternary ammonium salt modification are combined. The photoinitiator not only can be compatible with photoplatemaking of pattern surfaces in various printing processes, but also can be used for bio-ink to realize 3D bio-printing, and has special significance for use scenes with higher requirements on biocompatibility; the method has great application potential and market promotion value in various high-end fields such as water-based coatings, medical dressings, food contact materials, biomedical hydrogel and the like.

The invention relates to a method for obtaining a dental prosthesis comprising a crown and a root. The method comprises at least one step of guiding undifferentiated cells or keratinocytes and at least one step of three-dimensional printing, preferably 3D bioprinting of the dental prosthesis. Furthermore, the invention relates to a dental prosthesis obtained using said method, as well as to bio-inks suitable for three-dimensional printing (3D), preferably for 3D bioprinting.

A heterogeneous component free synthetic bio-ink formulation comprising a polymer derived from poly (asparagine) (PASPAm) for 3D bioprinting applications, a method of producing a three-dimensional object using the synthetic bio-ink formulation, and a three-dimensional object obtainable by the method are provided.

This relates to a hydrogel precursor composition and to a bioink obtainable from the hydrogel precursor composition, as well as a method of preparing and using the same. More specifically, This also relates to a bioink that contains a catechol grafted and spontaneously crosslinked hydrogel including cells, and to its use in 3D bioprinting and to a method of preparing the same.

The application discloses a 3D bioprinted radial porous scaffold for staged bone regeneration and a preparation method thereof, the 3D bioprinted radial porous scaffold can provide a biomimetic cell microenvironment to support the infiltration of host repair cells to a defect site, and promote bone integration after implantation, in addition, the sequential release of double growth factors and the sustained release of PO4 3‑ and Ca 2+ in the 3D bioprinted radial porous scaffold can complete the three key steps of staged bone regeneration, can realize efficient bone regeneration, and represents a promising biomimetic staged regeneration strategy.

The invention relates to a method for functionalizing a biopolymer, in which a biopolymer having-OH or-NH2 groups is reacted with at least one compound containing at least one C = C or C = C bond and a UV-Vis radiation-absorbing chromophore directly adjacent to the bond. The invention also relates to a process for obtaining a solid biopolymer material, wherein the functionalized biopolymer obtained by the above process undergoes reversible crosslinking upon exposure to light in the UV-VIS range. The invention also relates to the use of the functionalized biopolymer as a support material for 3D bioprinting and to the use of the functionalized biopolymer for producing a structure selected from spheroids, organoids, artificial organs, envelopes and tissue models.

This invention provides a water-soluble synthetic polymer bio-ink, comprising a dispersed phase and a dispersant mixed together. The dispersant is water, and the dispersed phase includes polymer microspheres, a crosslinking agent, and a UV photoinitiator. The polymer microspheres are made of a synthetic polymer material modified with double bonds at both ends. This water-soluble synthetic polymer bio-ink can be used for 3D bioprinting in a water-soluble system. This invention also provides a method for preparing the water-soluble synthetic polymer bio-ink, which is simple, low-cost, and suitable for industrial production. Furthermore, this invention provides an application of the water-soluble synthetic polymer bio-ink, which allows for 3D bioprinting and solidification in a water-soluble system to obtain a structurally stable and well-formed 3D printed scaffold. Cell fluid can also be added to the water-soluble synthetic polymer bio-ink to print a cell-containing 3D printed scaffold.

A method of rapid constructing human cerebral cortical organoids by 3D bioprinting and an application including preparing microfluidic chips, preparation of hydrogel of human cerebral cortical organoids, and printing of human cerebral cortical organoids. The microfluidic chip comprises a mixed-flow channel layer, liquid pool layer, microporous array layer, human cerebral cortical organoid culture layer, and culture medium recovery layer; the human cerebral cortical organoid hydrogel has gelatin, alginate, and hyaluronic acid; printing directly human cerebral cortical organoids in microfluidic chips by FRESH printing method, obtaining human cerebral cortical organoid chips after packaging. The application directly constructs large-scale human cerebral cortex-like with three layers of mutually connected structures in situ in organ chip through 3D bioprinting, simulates cerebrospinal fluid circulation through perfusion culture.

The invention provides a bone organ scaffold and a manufacturing method thereof, and relates to the technical field of bone organ scaffolds. The bracket comprises a plurality of functional layers which are arranged in sequence; each functional layer comprises a plurality of through holes in the transverse direction; the through holes of different functional layers correspond to one another in the longitudinal direction to form a plurality of first channels penetrating through the bracket; each functional layer comprises an upper part and a lower part in the longitudinal direction; the lower half part is a solid matrix layer; the upper half part is a filling layer; the filling layer comprises a plurality of solid matrix strips which are uniformly distributed; a gap is reserved between every two adjacent solid matrix strips to form a second channel for connecting the periphery of the functional layer with the first channel. According to the scheme, rapid production and manufacturing can be carried out through a 3D biological printing technology, and the manufacturing period of the stent is shortened; the biomimetic mineralization and neovascularization coupled bone organ designed by the scheme can be implanted into a femoral head necrosis area in the early stage of collapse in combination with a medullary decompression technology, and sufficient mechanical support and a sufficient blood supply reconstruction and bone repair environment are provided.

Proposed is a bioink supply system and, more particularly, proposed is a bioink supply system including: a hydrogel storage part; cell storage part; a mixing part configured to receive and mix a hydrogel and a cell solution from the hydrogel storage part and the cell storage part; a sensor part configured to measure a level of bioink inside a syringe; and a controller configured to receive a signal from the sensor part and maintain a constant level of the bioink inside the syringe, in which the mixing part supplies, to the syringe, the bioink prepared by mixing the hydrogel and the cell solution. The bioink supply system can continuously supply an active bioink to a syringe of a bioprinter during 3D bioprinting, and thus can continuously print large-scale biotissue, a plurality of organoids, organ-on-a-chip devices, etc.

The present invention discloses an AML organoid model and a construction method thereof, relating to the field of microfluidic chip technology. The present invention includes an AML organoid model, which is formed by 3D bioprinting and is constructed from top to bottom with a nutrient circulation mechanism, a tumor cell growth mechanism, a cell external stimulation mechanism, and an endothelial cell growth mechanism; a first flow channel is constructed in the nutrient circulation mechanism, and a first test electrode is installed in the first flow channel; a second flow channel is constructed in the tumor cell growth mechanism, and the second flow channel is filled with channel ink and a second test electrode is installed in the second flow channel; a cavity and an air supply channel are interconnected in the cell external stimulation mechanism, and the air supply channel is connected to the cavity; a third flow channel is constructed in the endothelial cell growth mechanism, and the inner wall of the third flow channel is filled with an outer layer of ink; so as to achieve the purpose of constructing a high-throughput drug sensitivity detection platform based on AML organoids by using microfluidics and 3D bioprinting technology for highly heterogeneous blood tumors such as AML.

A bioprinting apparatus 100 for 3D printing a model comprises a printing nozzle 115 for dispensing a magnetic or magnetisable bioink for forming the model; a bath 130 containing a model-supporting flu

The invention relates to 3D biological printing, injectable hydrogel, cell suspension culture and three-dimensional amplification, in particular to a preparation method of fusiform bioactive microgel capable of being used for injection, an obtained product and application. The fusiform microgel is synthesized for the first time, the fusiform microgel can be used as a cell adhesion medium after being subjected to surface bioactive group or protein modification, and spreading of cells in a three-dimensional environment can be achieved when the fusiform microgel is mixed with other hydrogels for use. Compared with traditional spherical microgel, the fusiform microgel has a large length-width ratio, namely the long diameter of the microgel is obviously larger than the short diameter of the microgel. The fusiform microgel is dispersed in other polymer solutions to form a microgel dispersion which can be used for extrusion-type hydrogel for biological printing or injection.