27 results about "3D bioprinting" patented technology

The present invention relates to a method for functionalizing a biopolymer having an -OH group or an -NH2 group by reacting the biopolymer with at least one compound having at least one C=C bond or C≡C bond and containing a chromophore that absorbs UV-Vis radiation directly adjacent to this bond. The present invention also relates to a method for obtaining a solid biopolymer material by reversibly crosslinking the functionalized biopolymer obtained by the above method under UV-Vis light irradiation. Furthermore, the present invention relates to the use of functionalized biopolymers as support materials for 3D bioprinting, or as materials for manufacturing structures selected from spheroids, organoids, artificial organs, coatings, and tissue models.

The invention relates to the technical field of biological engineering, and discloses a method for enhancing the urea circulation function of a 3D biological printing liver organ, which comprises the following steps: a cell preparation step: through a genetic engineering technology, enabling mitochondria of liver cells to express a light-driven proton pump, and enabling intracellular expression of a fluorescent probe for reporting ammonia concentration; an organ construction step: mixing the cells with hydrogel to prepare bio-ink, and constructing a three-dimensional liver organ by using a 3D biological printing technology; a system integration step: placing the liver organ in an integrated photoelectric regulation and control system capable of monitoring a fluorescence signal in real time and applying illumination; and a closed-loop enhancement step: the system monitors the ammonia concentration in real time, and when the ammonia concentration exceeds a preset threshold value, illumination is automatically applied to activate the proton pump, so that the urea circulation function is enhanced as required. Through closed-loop light control and a subcellular organelle energy channel, on-demand, efficient and high-temporal-spatial-resolution dynamic enhancement of the liver organ urea circulation function is achieved.

The invention discloses a quorum sensing quenching enzyme immobilization method of a 3D bio-printing self-assembled nanoflower enzyme-loaded scaffold and application of the quorum sensing quenching enzyme immobilization method. A quorum sensing quenching enzyme YtnP from deep sea is immobilized in an alginate matrix through a 3D printing technology to prepare a QQ scaffold, and the characterization, durability and antifouling performance of a 3D printing composite scaffold material are evaluated. The nanoflower enzyme-loaded scaffold has a continuous AHL degradation effect, and the AHL removal rate exceeds 99.98%. After 220 hours, the compound still has a remarkable inhibiting effect on biological pollution of the raw water PVDF membrane. The quorum sensing quenching enzyme 3D printing support carrying the self-assembled nanoflowers can replace a feeding channel grating, and the service life of a filter membrane subjected to biological scaling in a bioreactor can be remarkably prolonged through biodegradation.

This invention belongs to the technical field of 3D printing of biological tissues and organs, and discloses a biomimetic valve multi-nozzle 3D bioprinted scaffold and its application. The scaffold, from bottom to top, comprises a firmly connected elastin layer, a polysaccharide layer, and a collagen fiber layer. Each layer is constructed by alternating 3D printing of at least two materials; one of these materials is a photocurable hydrogel material providing mechanical support, and the other is a photocurable hydrogel material mixed with bioactive substances. Furthermore, the bioactive substances used in the elastin layer, polysaccharide layer, and collagen fiber layer are different from each other. This invention improves the structure and composition of the 3D bioprinted scaffold, creating a biomimetic three-layer structure and composition for a valve. The scaffold, designed with a three-layer structure of elastin, polysaccharide, and collagen fiber layers, provides cells with a specific sensory microenvironment, stimulating them to secrete corresponding extracellular matrix and completing extracellular matrix remodeling.

Disclosed herein are 3D printed vascular tissues and compositions and methods for making the 3D printed vascular tissues. The 3D printed vascular tissues can be used to model vascular tissue damage (e.g., vascular leakage) and in screening assays for agents that cause or inhibit or treat vascular tissue damage.

Aspects of the disclosure include a fabrication platform for supporting a bioprinted fiber structures during printing, patterning, and / or processing, comprising a frame with a plurality of posts for securing a cross-linkable fiber during printing thereof, and where a continuous length of the cross-linkable fiber is printed around a plurality of posts during the 3D bioprinting process. The fabrication platform enables the cross-linkable fiber to be suspended during one or more of printing, patterning, and / or processing. In this way, the bioprinted fiber structure comprises a uniform outer surface, and can be easily modified and / or further processed after printing and patterning are completed.

The invention relates to an aqueous composition comprising thiolated and norbornene-modified cold- water species-derived gelatine. A further aspect of the invention relates to a method to generate a three-dimensional hydrogel object, particularly an in vitro model for disease modelling / drug testing or an implant, comprising providing a composition according to the invention and forming the hydrogel object by bioprinting (extrusion-based or digital light-based (DLP)) or molding / casting to provide the three-dimensional hydrogel object. Also provided are a hydrogel implant obtained by a method according to the invention, and a kit comprising dried thiolated and norbornene-modified cold-water marine species gelatine.

The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

A controller (300, figure 4) for a 3D bioprinter 10, the controller being operable to control the functioning of the 3D bioprinter housed within an internal area of an enclosure 100, to produce a 3D p

A heterogeneous component free synthetic bio-ink formulation comprising a polymer derived from poly (asparagine) (PASPAm) for 3D bioprinting applications, a method of producing a three-dimensional object using the synthetic bio-ink formulation, and a three-dimensional object obtainable by the method are provided.

This relates to a hydrogel precursor composition and to a bioink obtainable from the hydrogel precursor composition, as well as a method of preparing and using the same. More specifically, This also relates to a bioink that contains a catechol grafted and spontaneously crosslinked hydrogel including cells, and to its use in 3D bioprinting and to a method of preparing the same.

The application discloses a 3D bioprinted radial porous scaffold for staged bone regeneration and a preparation method thereof, the 3D bioprinted radial porous scaffold can provide a biomimetic cell microenvironment to support the infiltration of host repair cells to a defect site, and promote bone integration after implantation, in addition, the sequential release of double growth factors and the sustained release of PO4 3‑ and Ca 2+ in the 3D bioprinted radial porous scaffold can complete the three key steps of staged bone regeneration, can realize efficient bone regeneration, and represents a promising biomimetic staged regeneration strategy.

This invention provides a water-soluble synthetic polymer bio-ink, comprising a dispersed phase and a dispersant mixed together. The dispersant is water, and the dispersed phase includes polymer microspheres, a crosslinking agent, and a UV photoinitiator. The polymer microspheres are made of a synthetic polymer material modified with double bonds at both ends. This water-soluble synthetic polymer bio-ink can be used for 3D bioprinting in a water-soluble system. This invention also provides a method for preparing the water-soluble synthetic polymer bio-ink, which is simple, low-cost, and suitable for industrial production. Furthermore, this invention provides an application of the water-soluble synthetic polymer bio-ink, which allows for 3D bioprinting and solidification in a water-soluble system to obtain a structurally stable and well-formed 3D printed scaffold. Cell fluid can also be added to the water-soluble synthetic polymer bio-ink to print a cell-containing 3D printed scaffold.

A method of rapid constructing human cerebral cortical organoids by 3D bioprinting and an application including preparing microfluidic chips, preparation of hydrogel of human cerebral cortical organoids, and printing of human cerebral cortical organoids. The microfluidic chip comprises a mixed-flow channel layer, liquid pool layer, microporous array layer, human cerebral cortical organoid culture layer, and culture medium recovery layer; the human cerebral cortical organoid hydrogel has gelatin, alginate, and hyaluronic acid; printing directly human cerebral cortical organoids in microfluidic chips by FRESH printing method, obtaining human cerebral cortical organoid chips after packaging. The application directly constructs large-scale human cerebral cortex-like with three layers of mutually connected structures in situ in organ chip through 3D bioprinting, simulates cerebrospinal fluid circulation through perfusion culture.

The invention provides a bone organ scaffold and a manufacturing method thereof, and relates to the technical field of bone organ scaffolds. The bracket comprises a plurality of functional layers which are arranged in sequence; each functional layer comprises a plurality of through holes in the transverse direction; the through holes of different functional layers correspond to one another in the longitudinal direction to form a plurality of first channels penetrating through the bracket; each functional layer comprises an upper part and a lower part in the longitudinal direction; the lower half part is a solid matrix layer; the upper half part is a filling layer; the filling layer comprises a plurality of solid matrix strips which are uniformly distributed; a gap is reserved between every two adjacent solid matrix strips to form a second channel for connecting the periphery of the functional layer with the first channel. According to the scheme, rapid production and manufacturing can be carried out through a 3D biological printing technology, and the manufacturing period of the stent is shortened; the biomimetic mineralization and neovascularization coupled bone organ designed by the scheme can be implanted into a femoral head necrosis area in the early stage of collapse in combination with a medullary decompression technology, and sufficient mechanical support and a sufficient blood supply reconstruction and bone repair environment are provided.

A bioprinting apparatus 100 for 3D printing a model comprises a printing nozzle 115 for dispensing a magnetic or magnetisable bioink for forming the model; a bath 130 containing a model-supporting flu

The invention relates to 3D biological printing, injectable hydrogel, cell suspension culture and three-dimensional amplification, in particular to a preparation method of fusiform bioactive microgel capable of being used for injection, an obtained product and application. The fusiform microgel is synthesized for the first time, the fusiform microgel can be used as a cell adhesion medium after being subjected to surface bioactive group or protein modification, and spreading of cells in a three-dimensional environment can be achieved when the fusiform microgel is mixed with other hydrogels for use. Compared with traditional spherical microgel, the fusiform microgel has a large length-width ratio, namely the long diameter of the microgel is obviously larger than the short diameter of the microgel. The fusiform microgel is dispersed in other polymer solutions to form a microgel dispersion which can be used for extrusion-type hydrogel for biological printing or injection.

The application discloses a 3D bioprinting bacteria-algae symbiotic engineering living material and a preparation method and application thereof. The method takes chlorella as a biological oxygen supply source, takes alkali-producing bacteria as phenol-degrading bacteria, takes sodium alginate and carboxymethyl cellulose as carriers, and respectively prepares bacterial bio-ink and algal bio-ink added with silicon dioxide, so as to build bacteria-algae symbiotic engineering living materials with stripe and layer configurations through a 3D bioprinting mechanism. The bacteria-algae symbiotic engineering living material realizes three-dimensional accurate arrangement and functional partition of bacteria and algae, and exhibits excellent degradation performance and photosynthetic performance under different phenol concentrations, and has application prospects in the biological degradation treatment of organic pollutants.

A bioink formulation for digital light processing bioprinting comprising a mixture of a biocompatible cleavable polymer precursor, a biocompatible non-cleavable polymer precursor, and a photoinitiator is described. Three-dimensional (3D) objects prepared using these bioink formulations are also described. In addition, a method of 3D bioprinting is described. The method includes providing a bioink formulation in a 3D bioprinter vat; repeatedly photoactivating the biocompatible photoactive polymer precursors in the 3D bioprinter vat on a build plate immersed in the vat to form a 3D bioprinted object comprising polymers having a series of predefined shapes across the vertical direction based on a set of sliced images; and treating the 3D bioprinted object with an agent that cleaves chemical bonds within the cleavable polymer.

Synthetic skin constructs are produced using 3D bioprinting and a cleaning process involving supercritical carbon dioxide (ScCO2). The ScCO2 process effectively removes all cellular materials, minimizing the risk of immune rejection and ensuring the skin constructs are safe and optimally effective.

The disclosed concept includes the preparation and use of biocompatible bioinks, as well as techniques and methods therein for bioprinting, preparing and 3D printing primary cells and patient-specific cells and forming functional soft tissue in a three-dimensional construct form that incorporates the cells and mimics native tissues. The biocompatible bioinks include alginate, methylcellulose, and primary or iPSC-derived cells in the form of single cells or aggregates. The bioprinted 3D construct includes one or more of the iPSC organoids, human islets, as well as TEPCs.

The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

This invention provides a 3D-printed bio-ink, a functional scaffold, and a method for preparing the same for bone defect repair. The 3D-printed bio-ink comprises patient-derived injectable platelet-rich fibrin (iPRF), autologous adipose-derived stem cells (ADSC), gelatin, and sodium alginate. This invention uses polycaprolactone (PCL) and hydroxyapatite (HA) as the load-bearing structural materials of the scaffold, and employs autologous iPRF solution and ADSC as the main active ingredients of the bio-ink. This results in good bioactivity while avoiding immune rejection. Furthermore, the prepared 3D bioprinted bone defect repair scaffold exhibits high mechanical strength and a strong ability to release growth factors, which is beneficial for inducing angiogenesis and osteogenic differentiation of stem cells, making it suitable for large-scale application.

The application discloses a cordyceps culture medium preparation method using yunnan rhizoma polygonati as the culture medium, and relates to the technical field of culture medium preparation.The method comprises the following steps: subjecting yunnan rhizoma polygonati raw materials to enzymolysis treatment to obtain an enzymolysis solution rich in small-molecule nutrients; extracting active components of yunnan rhizoma polygonati to prepare microcapsules with a response release characteristic; respectively using the enzymolysis solution and components containing the microcapsules and yunnan rhizoma polygonati materials without enzymolysis to prepare at least two 3D bioprinting inks; and layer-by-layer constructing a three-dimensional culture medium with a layered or gradient structure by using a 3D printing technology, and performing solidification.The culture medium prepared by the method can realize gradient distribution of nutrients and programmed release of active components, and can provide an optimized microenvironment for growth of cordyceps, so that the culture efficiency, yield and quality of the cordyceps are effectively improved.

The present invention relates to a method for obtaining a denture including a crown and a root. The method comprises a step of inducing at least one undifferentiated or keratinized cell, and a three-dimensional printing step, preferably a 3D bioprinting step of the denture. Furthermore, the present invention relates to a denture obtained by the method, and a bioink suitable for three-dimensional (3D) printing, preferably suitable for 3D bioprinting.

This invention discloses a deep in-situ 3D bioprinting system and method based on spherical standing wave focused ultrasound. It includes a spherical focusing system for generating spherical standing wave focused ultrasound, a motion control system for controlling the focal zone position of the focused ultrasound, and a data processing system for acquiring and analyzing ultrasonic cavitation signals to obtain control signals. The spherical standing wave focused ultrasound provided by this invention has superior focusing performance compared to traveling wave focused ultrasound, enabling spherical focused ultrasound 3D printing while balancing printing depth and accuracy, and exhibiting less focal shift in complex tissues. The sphere is formed by two hemispherical transducers of the same size and opposite positions, creating a spherical standing wave focusing cavity. The focused sound field is composed of sound waves directly radiated from the spherical surface and sound waves reflected multiple times by the spherical surface. When the sound waves directly focused at the center of the sphere and the sound waves reflected in each subsequent reflection are in phase, a very high sound pressure can be generated at the center of the sphere, and the focal zone accuracy is improved.

The application belongs to the technical field of natural polymer material modification and application, and discloses a 3D biological printing matrix material based on corn alcohol-soluble protein and a preparation method and application thereof, and particularly relates to a corn alcohol-soluble protein and a graft of pluronic F127 and a preparation method and application thereof in 3D biological printing. The material is obtained by the reaction of terminal alkyne pluronic F127 and poly-peptide modified corn alcohol-soluble protein. The application provides a 3D biological printing matrix material based on corn alcohol-soluble protein and pluronic F127, which has excellent biocompatibility, and the biological scaffold material prepared by using the material can realize excellent cell adhesion and proliferation; the low gel concentration solves the human body hazard of high concentration of F127; the temperature-sensitive characteristics make the material be in a flowable sol state at room temperature and be in a gel state at body temperature, and the biological scaffold material based on the material is formed and stably exists in a body temperature environment.