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3D bioprinting nano cellulose hydrogel ink

A nanocellulose, bioprinting technology, applied in inks, drug delivery, applications, etc., can solve the problem of adsorption and encapsulation of cells, inefficiency in transporting nutrients and excretion of metabolites, mechanical properties of hydrogel inks, and biocompatibility. In order to achieve the effect of good cell adhesion, low price and good biodegradability

Inactive Publication Date: 2019-02-26
深圳市康杰尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are certain disadvantages in the use of existing gel inks. (1) The same type of 3D printing and hydrogel materials used in tissue engineering have both developed to a certain stage, but the integration of the two has not yet been completed. For bioprinted cellulose materials, most of the applied materials are still in the basic research stage. It is necessary to consider the physical, chemical and biological properties of the material, and also to solve the problem of "printing" molding, which is still a long way from practical application; (2 ), the mechanical properties, biocompatibility and biodegradability of existing hydrogel inks need to be optimized, and the efficiency of absorbing and wrapping cells, transporting nutrients and excreting metabolites is not high. Therefore, we propose a 3D ink Nanocellulose hydrogel ink for bioprinting

Method used

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  • 3D bioprinting nano cellulose hydrogel ink

Examples

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Comparison scheme
Effect test

Embodiment 1

[0025] A 3D bioprinting nanocellulose hydrogel ink is made from the following components in parts by weight: 1100 parts of nanocellulose, 900 parts of hemicellulose, 1250 parts of ultrapure water, 380 parts of cell growth factor FGF2, and 175 parts of EGF , 225 copies of VEGF, 315 copies of collagen, 165 copies of fibrinogen, 60 copies of hyaluronic acid, and 700 copies of ink forming agent.

[0026] The preparation method of 3D bioprinting nanocellulose hydrogel ink includes the following steps:

[0027] (1) First, uniformly mix nanocellulose and hemicellulose, and let stand to obtain a mixture, stir with a glass rod, control the temperature at 60 °C, and let stand for 5 hours;

[0028] (2), adding ultrapure water to the mixture obtained in step (1) and soaking, then filtering the mixture, soaking for 1.5h, and controlling the temperature at 23°C;

[0029] (3) After filtering the mixture, add cell growth factors FGF2, EGF, VEGF, collagen, fibrinogen and hyaluronic acid in se...

Embodiment 2

[0032] A 3D bioprinting nanocellulose hydrogel ink is made from the following components in parts by weight: 1100 parts of nanocellulose, 900 parts of hemicellulose, 1250 parts of ultrapure water, 380 parts of cell growth factor FGF2, and 175 parts of EGF , 225 copies of VEGF, 315 copies of collagen, 165 copies of fibrinogen, 60 copies of hyaluronic acid, and 700 copies of ink forming agent.

[0033] The preparation method of 3D bioprinting nanocellulose hydrogel ink includes the following steps:

[0034] (1) First, uniformly mix nanocellulose and hemicellulose, and let stand to obtain a mixture, stir with a glass rod, control the temperature at 65 °C, and let stand for 5 hours;

[0035] (2), adding ultrapure water to the mixture obtained in step (1) and soaking, then filtering the mixture, soaking for 1.5h, and controlling the temperature at 23°C;

[0036] (3) After filtering the mixture, add cell growth factors FGF2, EGF, VEGF, collagen, fibrinogen and hyaluronic acid in tu...

Embodiment 3

[0039] A 3D bioprinting nanocellulose hydrogel ink is made of the following components by weight: 1100 parts of nanocellulose, 900 parts of hemicellulose, 1250 parts of ultrapure water, 380 parts of cell growth factor FGF2, and 175 parts of EGF , 225 copies of VEGF, 315 copies of collagen, 165 copies of fibrinogen, 60 copies of hyaluronic acid, and 700 copies of ink forming agent.

[0040] The preparation method of 3D bioprinting nanocellulose hydrogel ink includes the following steps:

[0041] (1) First, uniformly mix nanocellulose and hemicellulose, and let stand to obtain a mixture, stir with a glass rod, control the temperature at 70 ° C, and let stand for 5 hours;

[0042] (2), adding ultrapure water to the mixture obtained in step (1) and soaking, then filtering the mixture, soaking for 1.5h, and controlling the temperature at 23°C;

[0043] (3) After filtering the mixture, add cell growth factors FGF2, EGF, VEGF, collagen, fibrinogen and hyaluronic acid in turn, mix at...

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Abstract

The invention discloses 3D bioprinting nano cellulose hydrogel ink comprising the following components, by weight, 1100 parts of nano cellulose, 900 parts of hemicellulose, 1250 parts of ultrapure water, 380 parts of cell growth factor FGF2, 175 parts of EGF, 225 parts of VEGF, 315 parts of collagen, 165 parts of fibrinogen, 60 parts of hyaluronic acid and 700 parts of ink forming agent X, a preparation method of the 3D bioprinting nano cellulose hydrogel ink includes the following steps: uniformly mixing the nanocellulose and hemicellulose and performing standing. The 3D bioprinting nano cellulose hydrogel ink has small human immunological rejection and good biodegradability, has suitable mechanical properties, and has complete functions and low price. In practical application, the stability, reliability, convenience, degradability and environmental friendliness can also meet the needs of users and bring better use prospects.

Description

technical field [0001] The invention relates to the field of gel ink, in particular to a 3D bioprinting nanocellulose hydrogel ink. Background technique [0002] 3D bioprinting is a technology that uses a 3D bioprinter to create a cell scaffold, and then seed cells into the scaffold to allow the cells to grow. At present, the commercial 3D bioprinter has two print heads, which can simultaneously print human cells and usable cells. Scaffolding hydrogel ink for cell growth; [0003] Existing gel inks have certain drawbacks in use. (1) The same type of 3D printing and hydrogel materials used in tissue engineering have developed to a certain stage, but the two have not yet been integrated to complete. For bioprinted cellulose materials, most of the applied materials are still in the basic research stage. It is necessary to consider the physicochemical and biological properties of the material, and to solve the problem of "printing" molding, which is still a long way from practi...

Claims

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Application Information

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IPC IPC(8): C09D11/30C09D11/38A61L27/50A61L27/52A61L27/20B33Y70/00
CPCA61L27/20A61L27/50A61L27/52C09D11/30C09D11/38B33Y70/00A61L2400/12C08L1/02
Inventor 王硕程芳陈红波
Owner 深圳市康杰尔生物科技有限公司
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